JPS5791191A - Production of cell bodies of high content of pyruvate kinase - Google Patents
Production of cell bodies of high content of pyruvate kinaseInfo
- Publication number
- JPS5791191A JPS5791191A JP16513780A JP16513780A JPS5791191A JP S5791191 A JPS5791191 A JP S5791191A JP 16513780 A JP16513780 A JP 16513780A JP 16513780 A JP16513780 A JP 16513780A JP S5791191 A JPS5791191 A JP S5791191A
- Authority
- JP
- Japan
- Prior art keywords
- pyruvate kinase
- atcc
- dilution
- cell bodies
- cultivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: The continuous cultivation of a thermophilic bacterium such as bacillus stearothermophilus is conducted under a specified dilution to produce cell bodies of high pyruvate kinase content per unit body.
CONSTITUTION: A thermophilic bacterium in bacillus capable of producing pyruvate kinase, e.g., bacillus stearothermophilus ATCC 7953, ATCC 8005, ATCC 10149 or NCA 1503, is cultured wherein the dilution D is kept so as to satisfy the equation[D is dilution (1/hr); μmax is the maximum growth rate of the bacterium under the conditions of the continuous cultivation (1/hr)]. The culture medium is a usual enriched one containing glucose, meat essence and other nutritients and the cultivation is effected aerobically at about 40W75°C. After the cells are crushed, the resultant enzyme solution is purified by fractionation and adsorption to isolate pyruvate kinase.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16513780A JPS5791191A (en) | 1980-11-21 | 1980-11-21 | Production of cell bodies of high content of pyruvate kinase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16513780A JPS5791191A (en) | 1980-11-21 | 1980-11-21 | Production of cell bodies of high content of pyruvate kinase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5791191A true JPS5791191A (en) | 1982-06-07 |
Family
ID=15806593
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP16513780A Pending JPS5791191A (en) | 1980-11-21 | 1980-11-21 | Production of cell bodies of high content of pyruvate kinase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5791191A (en) |
-
1980
- 1980-11-21 JP JP16513780A patent/JPS5791191A/en active Pending
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