JPS5648891A - Preparation of polysaccharide - Google Patents
Preparation of polysaccharideInfo
- Publication number
- JPS5648891A JPS5648891A JP12262679A JP12262679A JPS5648891A JP S5648891 A JPS5648891 A JP S5648891A JP 12262679 A JP12262679 A JP 12262679A JP 12262679 A JP12262679 A JP 12262679A JP S5648891 A JPS5648891 A JP S5648891A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- polysaccharide
- methanol
- added
- cultivating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
PURPOSE: To prepare a polysaccharide in high yield, by cultivating a new strain, Pseudomonas sp. GD-96, belonging to the genus Pseudomonas, in a bacterium cultivating medium comprising methanol as a carbon source.
CONSTITUTION: Methanol as a main carbon source is added to a medium comprising a nitrogen source, e.g., NH4NO3, KH2PO4, MgSO4, CaCl2, vitamin, etc. The strain Pseudomonas sp. GD-96(FERM-P 5194) is inoculated on a medium to which methanol is added, and cultivated usually under aerobic conditions. For example, the cultivation is carried out by cultivating under agitation or agitation with aeration in a pH of the medium 6.5W7.5. After cultivation, the culure solution is diluted by 10W20 times, the strain and solid substance are removed by filtration or centrifugation, double amount of acetone is added to give a white fibrous crude polysaccharide, which is purified and dried under freezing to give a white spongy purified polysaccharide.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12262679A JPS5648891A (en) | 1979-09-26 | 1979-09-26 | Preparation of polysaccharide |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12262679A JPS5648891A (en) | 1979-09-26 | 1979-09-26 | Preparation of polysaccharide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5648891A true JPS5648891A (en) | 1981-05-02 |
Family
ID=14840612
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12262679A Pending JPS5648891A (en) | 1979-09-26 | 1979-09-26 | Preparation of polysaccharide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5648891A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0231585A2 (en) * | 1986-02-06 | 1987-08-12 | Celgene Corporation | Biosynthesis of heteropolysaccharides |
EP0232582A2 (en) * | 1986-02-06 | 1987-08-19 | Celgene Corporation | Novel species of facultative methylotrophic bacterium |
-
1979
- 1979-09-26 JP JP12262679A patent/JPS5648891A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0231585A2 (en) * | 1986-02-06 | 1987-08-12 | Celgene Corporation | Biosynthesis of heteropolysaccharides |
EP0232582A2 (en) * | 1986-02-06 | 1987-08-19 | Celgene Corporation | Novel species of facultative methylotrophic bacterium |
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