JPS56126758A - Enzyme electrode - Google Patents

Enzyme electrode

Info

Publication number
JPS56126758A
JPS56126758A JP2983080A JP2983080A JPS56126758A JP S56126758 A JPS56126758 A JP S56126758A JP 2983080 A JP2983080 A JP 2983080A JP 2983080 A JP2983080 A JP 2983080A JP S56126758 A JPS56126758 A JP S56126758A
Authority
JP
Japan
Prior art keywords
electrode
measured
liquid
enzyme
film
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2983080A
Other languages
Japanese (ja)
Other versions
JPS6257941B2 (en
Inventor
Hisayuki Ikeda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yokogawa Electric Corp
Original Assignee
Yokogawa Hokushin Electric Corp
Yokogawa Electric Works Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yokogawa Hokushin Electric Corp, Yokogawa Electric Works Ltd filed Critical Yokogawa Hokushin Electric Corp
Priority to JP2983080A priority Critical patent/JPS56126758A/en
Publication of JPS56126758A publication Critical patent/JPS56126758A/en
Publication of JPS6257941B2 publication Critical patent/JPS6257941B2/ja
Granted legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE:To prepare a simple low-cost enzyme electrode not requiring a pump for supplying oxygen, etc. by providing negative and positive electrode parts holding an electrolytic-solution retaining part between them and engaging with each other, a fixed-enzyme film on one side of the electrode and a hydrophobic processing film on the other side of the electrode through the intermediary of a porous substrate. CONSTITUTION:The anode 21 of Pt or the like and the cathode 23 of Ag or the like are arranged, holding the electrolytic-solution retaining part 22 impregnated with electrolytic solution between them and engaging with each other, and are connected with lead wires 20a and 20b respectively. On one side of the above electrode part is provided the fixed-enzyme film 26 and on the other side thereof are formed the porous substrate 24 of Al2O3, etc. and the hydrophobic processing film 25 prepared by silicone rubber coating or other measure, sequentially. The enzyme electrode 33 thus prepared is put in a vessel for the liquid 34 to be measured, provided with a part 35 for supplying the liquid to be measured and a part 36 for discharging the liquid to be measured, and the concentration of the matrix in the liquid 34 to be measured is measured by a measuring apparatus having a power source 31 and an ammeter 32. Since resolved O2 in the liquid 34 permeates through the film 25 and is supplied into the enzyme electrode, the pump for supplying O2, etc. becomes unnecessary.
JP2983080A 1980-03-11 1980-03-11 Enzyme electrode Granted JPS56126758A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2983080A JPS56126758A (en) 1980-03-11 1980-03-11 Enzyme electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2983080A JPS56126758A (en) 1980-03-11 1980-03-11 Enzyme electrode

Publications (2)

Publication Number Publication Date
JPS56126758A true JPS56126758A (en) 1981-10-05
JPS6257941B2 JPS6257941B2 (en) 1987-12-03

Family

ID=12286938

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2983080A Granted JPS56126758A (en) 1980-03-11 1980-03-11 Enzyme electrode

Country Status (1)

Country Link
JP (1) JPS56126758A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59166852A (en) * 1983-03-11 1984-09-20 Matsushita Electric Ind Co Ltd Biosensor
JPS61262652A (en) * 1985-05-17 1986-11-20 Nok Corp Oxygen sensor
JPS6232351A (en) * 1985-08-06 1987-02-12 Nok Corp Enzyme sensor
JP2002296217A (en) * 2001-03-29 2002-10-09 Matsushita Electric Ind Co Ltd Biosensor
JP4517498B2 (en) * 2000-11-24 2010-08-04 パナソニック株式会社 Biosensor
JP4649767B2 (en) * 2001-05-07 2011-03-16 パナソニック株式会社 Biosensor

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59166852A (en) * 1983-03-11 1984-09-20 Matsushita Electric Ind Co Ltd Biosensor
JPH046907B2 (en) * 1983-03-11 1992-02-07 Matsushita Electric Ind Co Ltd
JPS61262652A (en) * 1985-05-17 1986-11-20 Nok Corp Oxygen sensor
JPS6232351A (en) * 1985-08-06 1987-02-12 Nok Corp Enzyme sensor
JP4517498B2 (en) * 2000-11-24 2010-08-04 パナソニック株式会社 Biosensor
JP2002296217A (en) * 2001-03-29 2002-10-09 Matsushita Electric Ind Co Ltd Biosensor
JP4649767B2 (en) * 2001-05-07 2011-03-16 パナソニック株式会社 Biosensor

Also Published As

Publication number Publication date
JPS6257941B2 (en) 1987-12-03

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