JPS5497491A - Measuring method of enzyme activity - Google Patents
Measuring method of enzyme activityInfo
- Publication number
- JPS5497491A JPS5497491A JP463878A JP463878A JPS5497491A JP S5497491 A JPS5497491 A JP S5497491A JP 463878 A JP463878 A JP 463878A JP 463878 A JP463878 A JP 463878A JP S5497491 A JPS5497491 A JP S5497491A
- Authority
- JP
- Japan
- Prior art keywords
- coenzyme
- electrode
- measuring
- sample
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To enable measurement of good accuracy to be made because of low activity of coenzyme itself and solve problems such as short working life by covering the end of measuring electrode with the fixed coenzyme film comprising fixing coenzyme to a porous high polymer material.
CONSTITUTION: A reference electrode block 1 provided with a reference elecrode 15 consisting of a porous member 17, electrolyte 18, Ag-AgCl electrode 19 being disposed in an opening part 16 and a working electrode 21 consisting of a copper plate 22, glassy carbon plate 23, fixed coenzyme film 24 are provided and are opposed to the working electrode block 2. A voltage is then applied thereto. The measuring sample and substrate-containing buffer solution in tanks 9, 10 are passed through the sample chamber 3 by a metering pump 12. The enzyme in the sample promotes reaction of substrate and coenzyme and lets the coenzyme to change to oxidized or reduced form but this coenzyme changes again to the original form owing to electrochemical circulating action. The change in the current of this time is measured with a measuring instrument 26 connected to the electrodes 15, 21 by lead wires 27.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53004638A JPS6052375B2 (en) | 1978-01-19 | 1978-01-19 | How to measure enzyme activity |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53004638A JPS6052375B2 (en) | 1978-01-19 | 1978-01-19 | How to measure enzyme activity |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5497491A true JPS5497491A (en) | 1979-08-01 |
JPS6052375B2 JPS6052375B2 (en) | 1985-11-19 |
Family
ID=11589532
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53004638A Expired JPS6052375B2 (en) | 1978-01-19 | 1978-01-19 | How to measure enzyme activity |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6052375B2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56107154A (en) * | 1979-12-04 | 1981-08-25 | Technicon Instr | Produced articles and enzyme activity measuring method* enzyme reaction control method* analyzer* reactor* automatic electrochemical analyzer* and thin film enzyme measuring sensor |
JPS6126851A (en) * | 1984-07-17 | 1986-02-06 | Snow Brand Milk Prod Co Ltd | Method and instrument for automatic measurement of glucose |
-
1978
- 1978-01-19 JP JP53004638A patent/JPS6052375B2/en not_active Expired
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56107154A (en) * | 1979-12-04 | 1981-08-25 | Technicon Instr | Produced articles and enzyme activity measuring method* enzyme reaction control method* analyzer* reactor* automatic electrochemical analyzer* and thin film enzyme measuring sensor |
JPS6355025B2 (en) * | 1979-12-04 | 1988-11-01 | Technicon Instr | |
JPS6126851A (en) * | 1984-07-17 | 1986-02-06 | Snow Brand Milk Prod Co Ltd | Method and instrument for automatic measurement of glucose |
JPH0462022B2 (en) * | 1984-07-17 | 1992-10-02 | Snow Brand Milk Prod Co Ltd |
Also Published As
Publication number | Publication date |
---|---|
JPS6052375B2 (en) | 1985-11-19 |
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