JPS5722551A - Method for measuring methane by microbiological electrode - Google Patents

Method for measuring methane by microbiological electrode

Info

Publication number
JPS5722551A
JPS5722551A JP9697280A JP9697280A JPS5722551A JP S5722551 A JPS5722551 A JP S5722551A JP 9697280 A JP9697280 A JP 9697280A JP 9697280 A JP9697280 A JP 9697280A JP S5722551 A JPS5722551 A JP S5722551A
Authority
JP
Japan
Prior art keywords
electrode
methane
microbiological
gas
oxygen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP9697280A
Other languages
Japanese (ja)
Inventor
Motohiko Hikima
Takeo Yasuda
Masao Karube
Shuichi Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP9697280A priority Critical patent/JPS5722551A/en
Publication of JPS5722551A publication Critical patent/JPS5722551A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Abstract

PURPOSE:To measure methane concentration quickly by contacting the microbiological electrode, in which methane utilizing bacteria are sealed, with gas to be measured, and sensing the reduction in dissolved oxygen in a solidified microbiological layer as an amount of reduction or reducing speed of the output current of the electrode. CONSTITUTION:In an outer tube 9, are contained platinum cathode 5, an alminum anode 6, and KCl elctrolyte 7. The microbiological electrode 10 is provided between a separating film 4 of an oxygen electrode and a dialysis film or gas transmitting film 3, and attached to a measuring cell 12. The methane utilizing bacteria 1 which oxidize methane and consume oxygen are sealed in the microbiological electrode 10. Air is sent into the cell 12. The gas to be measured is injected from an injecting port 16 for 1-5min at every 4--20min interval, dilluted by air, and contacted with the electrode 10. Methane is diffused in the bacteria 1, and oxidized by the bacteria 1. Oxygen is consumed and the concentration of the dissolved oxygen in the vicinity of the separating film 4 is reduced. Therefore the output current of the oxygen electrode is decreased and recorded in a recorder 13. The relationship between the methane concentration of the gas to be measured and the reduced value of the current has been obtained for standard methane gas, and the methane concentration is obtained from the height of the peak.
JP9697280A 1980-07-16 1980-07-16 Method for measuring methane by microbiological electrode Pending JPS5722551A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9697280A JPS5722551A (en) 1980-07-16 1980-07-16 Method for measuring methane by microbiological electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9697280A JPS5722551A (en) 1980-07-16 1980-07-16 Method for measuring methane by microbiological electrode

Publications (1)

Publication Number Publication Date
JPS5722551A true JPS5722551A (en) 1982-02-05

Family

ID=14179128

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9697280A Pending JPS5722551A (en) 1980-07-16 1980-07-16 Method for measuring methane by microbiological electrode

Country Status (1)

Country Link
JP (1) JPS5722551A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63250182A (en) * 1987-04-06 1988-10-18 Nec Corp Photo-interrupter
JP2009258024A (en) * 2008-04-18 2009-11-05 Yazaki Corp Gas sensor
JP2013513125A (en) * 2009-12-08 2013-04-18 カンブリアン イノベーション エルエルシー Microorganism-based sensor for environmental monitoring
EP3284829A1 (en) * 2009-06-16 2018-02-21 Cambrian Innovation, Inc. Systems and devices for treating and monitoring water, wastewater and other biodegradable matter
US11150213B2 (en) 2011-06-14 2021-10-19 Cambrian Innovation Inc. Biological oxygen demand sensors

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63250182A (en) * 1987-04-06 1988-10-18 Nec Corp Photo-interrupter
JP2009258024A (en) * 2008-04-18 2009-11-05 Yazaki Corp Gas sensor
EP3284829A1 (en) * 2009-06-16 2018-02-21 Cambrian Innovation, Inc. Systems and devices for treating and monitoring water, wastewater and other biodegradable matter
JP2013513125A (en) * 2009-12-08 2013-04-18 カンブリアン イノベーション エルエルシー Microorganism-based sensor for environmental monitoring
US9046478B2 (en) 2009-12-08 2015-06-02 Cambrian Innovation Inc. Microbially-based sensors for environmental monitoring
EP2510345A4 (en) * 2009-12-08 2015-07-08 Cambrian Innovation Inc Microbially-based sensors for environmental monitoring
JP2015158520A (en) * 2009-12-08 2015-09-03 カンブリアン イノベーション エルエルシー Microbially-based sensors for environmental monitoring
US9551685B2 (en) 2009-12-08 2017-01-24 Cambrian Innovation Inc. Microbially-based sensors for environmental monitoring
US11150213B2 (en) 2011-06-14 2021-10-19 Cambrian Innovation Inc. Biological oxygen demand sensors

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