JPS5766351A - Measuring method for nitrous acid - Google Patents
Measuring method for nitrous acidInfo
- Publication number
- JPS5766351A JPS5766351A JP55141468A JP14146880A JPS5766351A JP S5766351 A JPS5766351 A JP S5766351A JP 55141468 A JP55141468 A JP 55141468A JP 14146880 A JP14146880 A JP 14146880A JP S5766351 A JPS5766351 A JP S5766351A
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- electrode
- oxygen
- nitrous acid
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Abstract
PURPOSE:To improve stability and reliability, by a method wherein a microorganism electrode is formed such that a nitrification bacillus, selectively oxidizing only nitrous acid into nitric acid, is attached to a diaphragm of a dissolved oxygen electrode, and the outside thereof is covered with a gas permeable film, and the microorganism electride is used. CONSTITUTION:A microorganism electrode 11 consists of a bacteriim laye 1 of microorganism, supporter 2, covering the bacterium layer 1 and supporting bacterium microorganism, a gas permeable film 3, a diaphragm 4 of an oxygen electrode, a platinum cathode 5, an aluminum anode 6, a potassium chloride electrolytic liquid 7 and the like. A microorganism bacterium is what is called a nitrification bacillus, and is nitric acid preparing bacterium, e.g., nitrobacter agiles ATCC 14123, which oxidizes nitrous acid. The way to measure nitrous acid by means of the electrode is that a pH of liquid to be measured is set below 5.0, and concentration of dissolved oxygen is set into a saturation or a state near a saturation by blowing oxygen or air. The microorganism electrode is brought into contact with the liquid to be measured. Under the coexistence of oxygen, the nitrification bacillus oxidizes the nitrous acid and consumes oxygen, which results in reducing the output current of the microorganism electrode.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55141468A JPS5766351A (en) | 1980-10-09 | 1980-10-09 | Measuring method for nitrous acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55141468A JPS5766351A (en) | 1980-10-09 | 1980-10-09 | Measuring method for nitrous acid |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5766351A true JPS5766351A (en) | 1982-04-22 |
Family
ID=15292578
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55141468A Pending JPS5766351A (en) | 1980-10-09 | 1980-10-09 | Measuring method for nitrous acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5766351A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109001364A (en) * | 2018-09-26 | 2018-12-14 | 华东师范大学 | A kind of binary channels sampling atmosphere HONO measurement system and method |
-
1980
- 1980-10-09 JP JP55141468A patent/JPS5766351A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109001364A (en) * | 2018-09-26 | 2018-12-14 | 华东师范大学 | A kind of binary channels sampling atmosphere HONO measurement system and method |
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