JPS5786752A - Measuring method for enzyme activity by electrode method - Google Patents
Measuring method for enzyme activity by electrode methodInfo
- Publication number
- JPS5786752A JPS5786752A JP55163536A JP16353680A JPS5786752A JP S5786752 A JPS5786752 A JP S5786752A JP 55163536 A JP55163536 A JP 55163536A JP 16353680 A JP16353680 A JP 16353680A JP S5786752 A JPS5786752 A JP S5786752A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- way valve
- electrode
- enzyme activity
- detected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To measure activity of an enzyme, by a method wherein a lactic acid is prepared in the presence of an enzyme to be measured, and the lactic acid is detected by an enzyme electrode in which a glycolic acid oxidase is fixed. CONSTITUTION:A 3-way valve 4 is mounted at a buffer liquid flow path 3 ranging from a buffer liquid tank 1 to a waste liquid tank 2, and one side of the 3- way valve 4 is coupled to a sample tank 5 in which a sample to be measured is stored. An enzyme electrode 6, in which a glycolic acid oxidase enzyme is fixed as a reaction catalyst and a porous high-molecular film is formed, and a micropump 7 are installed at the downstream side of the 3-way valve 4. The reduction in oxygen concentration is detected as a change in current by the enzyme electrode 6, and the signal is sent to a diffenrentiator 9. A differential value, obtained from the differentiator 9, represents an enzyme activity and is recorded in a recorder 10.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55163536A JPS5786752A (en) | 1980-11-19 | 1980-11-19 | Measuring method for enzyme activity by electrode method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55163536A JPS5786752A (en) | 1980-11-19 | 1980-11-19 | Measuring method for enzyme activity by electrode method |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5786752A true JPS5786752A (en) | 1982-05-29 |
Family
ID=15775739
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55163536A Pending JPS5786752A (en) | 1980-11-19 | 1980-11-19 | Measuring method for enzyme activity by electrode method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5786752A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60119459A (en) * | 1983-11-29 | 1985-06-26 | Agency Of Ind Science & Technol | Method for measuring concentration of adenosine 5'-diphosphate and enzyme activity of enzyme forming adenosine 5'-diphosphate |
-
1980
- 1980-11-19 JP JP55163536A patent/JPS5786752A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60119459A (en) * | 1983-11-29 | 1985-06-26 | Agency Of Ind Science & Technol | Method for measuring concentration of adenosine 5'-diphosphate and enzyme activity of enzyme forming adenosine 5'-diphosphate |
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