JPS5786752A - Measuring method for enzyme activity by electrode method - Google Patents

Measuring method for enzyme activity by electrode method

Info

Publication number
JPS5786752A
JPS5786752A JP55163536A JP16353680A JPS5786752A JP S5786752 A JPS5786752 A JP S5786752A JP 55163536 A JP55163536 A JP 55163536A JP 16353680 A JP16353680 A JP 16353680A JP S5786752 A JPS5786752 A JP S5786752A
Authority
JP
Japan
Prior art keywords
enzyme
way valve
electrode
enzyme activity
detected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55163536A
Other languages
Japanese (ja)
Inventor
Koichi Takizawa
Tomoo Kaede
Satoshi Nakajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Omron Corp
Original Assignee
Tateisi Electronics Co
Omron Tateisi Electronics Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tateisi Electronics Co, Omron Tateisi Electronics Co filed Critical Tateisi Electronics Co
Priority to JP55163536A priority Critical patent/JPS5786752A/en
Publication of JPS5786752A publication Critical patent/JPS5786752A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Abstract

PURPOSE:To measure activity of an enzyme, by a method wherein a lactic acid is prepared in the presence of an enzyme to be measured, and the lactic acid is detected by an enzyme electrode in which a glycolic acid oxidase is fixed. CONSTITUTION:A 3-way valve 4 is mounted at a buffer liquid flow path 3 ranging from a buffer liquid tank 1 to a waste liquid tank 2, and one side of the 3- way valve 4 is coupled to a sample tank 5 in which a sample to be measured is stored. An enzyme electrode 6, in which a glycolic acid oxidase enzyme is fixed as a reaction catalyst and a porous high-molecular film is formed, and a micropump 7 are installed at the downstream side of the 3-way valve 4. The reduction in oxygen concentration is detected as a change in current by the enzyme electrode 6, and the signal is sent to a diffenrentiator 9. A differential value, obtained from the differentiator 9, represents an enzyme activity and is recorded in a recorder 10.
JP55163536A 1980-11-19 1980-11-19 Measuring method for enzyme activity by electrode method Pending JPS5786752A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55163536A JPS5786752A (en) 1980-11-19 1980-11-19 Measuring method for enzyme activity by electrode method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55163536A JPS5786752A (en) 1980-11-19 1980-11-19 Measuring method for enzyme activity by electrode method

Publications (1)

Publication Number Publication Date
JPS5786752A true JPS5786752A (en) 1982-05-29

Family

ID=15775739

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55163536A Pending JPS5786752A (en) 1980-11-19 1980-11-19 Measuring method for enzyme activity by electrode method

Country Status (1)

Country Link
JP (1) JPS5786752A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60119459A (en) * 1983-11-29 1985-06-26 Agency Of Ind Science & Technol Method for measuring concentration of adenosine 5'-diphosphate and enzyme activity of enzyme forming adenosine 5'-diphosphate

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60119459A (en) * 1983-11-29 1985-06-26 Agency Of Ind Science & Technol Method for measuring concentration of adenosine 5'-diphosphate and enzyme activity of enzyme forming adenosine 5'-diphosphate

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