JPS5786040A - Method for determination of biochemical material by electrode method - Google Patents

Method for determination of biochemical material by electrode method

Info

Publication number
JPS5786040A
JPS5786040A JP55162283A JP16228380A JPS5786040A JP S5786040 A JPS5786040 A JP S5786040A JP 55162283 A JP55162283 A JP 55162283A JP 16228380 A JP16228380 A JP 16228380A JP S5786040 A JPS5786040 A JP S5786040A
Authority
JP
Japan
Prior art keywords
enzyme
immobilized
electrode
film
lactic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55162283A
Other languages
Japanese (ja)
Inventor
Koichi Takizawa
Kunio Kaede
Satoshi Nakajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Omron Corp
Original Assignee
Tateisi Electronics Co
Omron Tateisi Electronics Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tateisi Electronics Co, Omron Tateisi Electronics Co filed Critical Tateisi Electronics Co
Priority to JP55162283A priority Critical patent/JPS5786040A/en
Publication of JPS5786040A publication Critical patent/JPS5786040A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Abstract

PURPOSE:To determine lactic acids by using an enzyme electrode contg. an immobilized enzyme membrane immobilized with lactic acid oxidase as a reaction catalyst. CONSTITUTION:An enzyme electrode 6 contains a housing 61, and holes through which a buffer flow passage 3 can penetrate are formed to the housing 61. An immobilized enzyme membrane 66 is also provided thereto. This membrane 66 contains a porous high polymer thin film 661 consisting of, for example, acetylcellulose or the like, and lactic acid oxidase as a reaction catalyst is immobilized in this thin film 661. One of surface sides of the film 661 is coated with a porous film 662 of polycarbonate or the like and the other surface side is coated with a permeable film 663 of a fluororesin or polyethylene permitting the permeation of only the enzyme that is measured by a working electrode 63 and a reference electrode 64. The conc. of lactic acid is determined by detecting the electric current between the electrodes changing in accordance with enzyme reaction.
JP55162283A 1980-11-17 1980-11-17 Method for determination of biochemical material by electrode method Pending JPS5786040A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55162283A JPS5786040A (en) 1980-11-17 1980-11-17 Method for determination of biochemical material by electrode method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55162283A JPS5786040A (en) 1980-11-17 1980-11-17 Method for determination of biochemical material by electrode method

Publications (1)

Publication Number Publication Date
JPS5786040A true JPS5786040A (en) 1982-05-28

Family

ID=15751531

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55162283A Pending JPS5786040A (en) 1980-11-17 1980-11-17 Method for determination of biochemical material by electrode method

Country Status (1)

Country Link
JP (1) JPS5786040A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2705687A1 (en) * 1993-05-28 1994-12-02 Inst Oenologie Identification, cloning and expression of the malolactic enzyme
CN104198556A (en) * 2014-08-11 2014-12-10 南京普朗医疗设备有限公司 Preparation method of electrode membrane based on lactate oxidase and product prepared by the method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2705687A1 (en) * 1993-05-28 1994-12-02 Inst Oenologie Identification, cloning and expression of the malolactic enzyme
CN104198556A (en) * 2014-08-11 2014-12-10 南京普朗医疗设备有限公司 Preparation method of electrode membrane based on lactate oxidase and product prepared by the method

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