JPS5548391A - Preparation of cytochrome oxidase - Google Patents
Preparation of cytochrome oxidaseInfo
- Publication number
- JPS5548391A JPS5548391A JP12280078A JP12280078A JPS5548391A JP S5548391 A JPS5548391 A JP S5548391A JP 12280078 A JP12280078 A JP 12280078A JP 12280078 A JP12280078 A JP 12280078A JP S5548391 A JPS5548391 A JP S5548391A
- Authority
- JP
- Japan
- Prior art keywords
- bodies
- enzyme
- heat
- crushed
- strains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To obtain the title heat-resistant enzyme useful as medical enzyme formulas and reagents for biochemical studies, industrially advantageously, by crushing bacterium bodies of Thermus thermophilus HB8 strains cultured in a medium, and by separating and collecting the enzyme.
CONSTITUTION: Thermus thermophilus HB strains, which belong to the genus Thermus, are cultured in a medium containing 0.5% of peptone, 0.4% of a yeast extract, 0.2% of NaCl, and 0.1% of glucose at 75°C for about 6hr, and collected in the latter logarithmic growth phase. The bacterium bodies thus obtained are crushed by sound waves, etc. The crushed bodies are purified by centrifugation, dialysis, ion exchange resin treatment, ultrafiltration, etc. to give the desired cytochrome oxidase, having the heat resistance and a residual activity of about 75% after heat-treatment at 78°C for 20min.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12280078A JPS5548391A (en) | 1978-10-05 | 1978-10-05 | Preparation of cytochrome oxidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12280078A JPS5548391A (en) | 1978-10-05 | 1978-10-05 | Preparation of cytochrome oxidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5548391A true JPS5548391A (en) | 1980-04-07 |
JPS6125352B2 JPS6125352B2 (en) | 1986-06-14 |
Family
ID=14844939
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12280078A Granted JPS5548391A (en) | 1978-10-05 | 1978-10-05 | Preparation of cytochrome oxidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5548391A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60263394A (en) * | 1984-06-08 | 1985-12-26 | Sharp Corp | Memory refresh method |
WO2002033057A3 (en) * | 2000-10-16 | 2002-10-03 | Basf Ag | Cytochrome p450 monooxygenases consisting of thermophilic bacteria |
-
1978
- 1978-10-05 JP JP12280078A patent/JPS5548391A/en active Granted
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60263394A (en) * | 1984-06-08 | 1985-12-26 | Sharp Corp | Memory refresh method |
WO2002033057A3 (en) * | 2000-10-16 | 2002-10-03 | Basf Ag | Cytochrome p450 monooxygenases consisting of thermophilic bacteria |
CN1330761C (en) * | 2000-10-16 | 2007-08-08 | 巴斯福股份公司 | Cytochrome P450 monooxygenases consisting of thermophilic bacteria |
Also Published As
Publication number | Publication date |
---|---|
JPS6125352B2 (en) | 1986-06-14 |
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