JPS5548393A - Preparation of c-type cytochrome - Google Patents

Preparation of c-type cytochrome

Info

Publication number
JPS5548393A
JPS5548393A JP12279978A JP12279978A JPS5548393A JP S5548393 A JPS5548393 A JP S5548393A JP 12279978 A JP12279978 A JP 12279978A JP 12279978 A JP12279978 A JP 12279978A JP S5548393 A JPS5548393 A JP S5548393A
Authority
JP
Japan
Prior art keywords
crushed
bodies
cytochrome
measured
strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12279978A
Other languages
Japanese (ja)
Other versions
JPS6212998B2 (en
Inventor
Yasuo Oshima
Yasuyoshi Motonami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP12279978A priority Critical patent/JPS5548393A/en
Publication of JPS5548393A publication Critical patent/JPS5548393A/en
Publication of JPS6212998B2 publication Critical patent/JPS6212998B2/ja
Granted legal-status Critical Current

Links

Abstract

PURPOSE: Cytochrome C555, 549 having a molecular weight of about 10,000, obtained by separating and purifying the crushed bacterium bodies of Thermus thermophilus HB8 strains cultured in a medium.
CONSTITUTION: Thermus thermophilus HB8 strains which belong to the genus Thermus are cultured in a medium containing 0.5% of peptone, 0.4% of a yeast extract, 0.2% of NaCl, and 0,1% of glucose at 75°c for about 6hr, and collected in the latter logarithrmic growth period. The bacterium bodies thus obtained are crushed by sound wave treatment, etc. The crushed bodies are purified by centrifugation, dialysis, ion exchange resin treatment, ultrafiltration, etc. to give the cytochrome C555, 549 having a moecular weight of 11,000 measured by sedimentation equilibrium and 10,000 measured by electrophoresis.
COPYRIGHT: (C)1980,JPO&Japio
JP12279978A 1978-10-05 1978-10-05 Preparation of c-type cytochrome Granted JPS5548393A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP12279978A JPS5548393A (en) 1978-10-05 1978-10-05 Preparation of c-type cytochrome

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12279978A JPS5548393A (en) 1978-10-05 1978-10-05 Preparation of c-type cytochrome

Publications (2)

Publication Number Publication Date
JPS5548393A true JPS5548393A (en) 1980-04-07
JPS6212998B2 JPS6212998B2 (en) 1987-03-23

Family

ID=14844914

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12279978A Granted JPS5548393A (en) 1978-10-05 1978-10-05 Preparation of c-type cytochrome

Country Status (1)

Country Link
JP (1) JPS5548393A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007259557A (en) * 2006-03-22 2007-10-04 Nissan Motor Co Ltd Laminated core structure for rotary electric machines

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63180999U (en) * 1987-05-13 1988-11-22

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007259557A (en) * 2006-03-22 2007-10-04 Nissan Motor Co Ltd Laminated core structure for rotary electric machines

Also Published As

Publication number Publication date
JPS6212998B2 (en) 1987-03-23

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