JPH11225797A - 血漿および／または血清中の高ウイルス濃度をポリメラーゼ連鎖反応を用いて検出する方法 - Google Patents
血漿および／または血清中の高ウイルス濃度をポリメラーゼ連鎖反応を用いて検出する方法Info
- Publication number
- JPH11225797A JPH11225797A JP10336431A JP33643198A JPH11225797A JP H11225797 A JPH11225797 A JP H11225797A JP 10336431 A JP10336431 A JP 10336431A JP 33643198 A JP33643198 A JP 33643198A JP H11225797 A JPH11225797 A JP H11225797A
- Authority
- JP
- Japan
- Prior art keywords
- dna
- parvovirus
- pcr
- amplification
- chain reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000003752 polymerase chain reaction Methods 0.000 title claims abstract description 32
- 241000700605 Viruses Species 0.000 title claims abstract description 14
- 238000001514 detection method Methods 0.000 title claims abstract description 11
- 210000002966 serum Anatomy 0.000 title claims abstract description 8
- 210000002381 plasma Anatomy 0.000 title abstract 3
- 241000125945 Protoparvovirus Species 0.000 claims abstract description 23
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 18
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 18
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 18
- 230000003321 amplification Effects 0.000 claims abstract description 16
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 16
- 230000035945 sensitivity Effects 0.000 claims abstract description 11
- 238000000137 annealing Methods 0.000 claims abstract description 6
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 33
- 239000000523 sample Substances 0.000 claims description 16
- 239000007850 fluorescent dye Substances 0.000 claims description 9
- 239000000975 dye Substances 0.000 claims description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 230000001717 pathogenic effect Effects 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims description 2
- 230000002616 endonucleolytic effect Effects 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims 1
- 241000702617 Human parvovirus B19 Species 0.000 abstract description 9
- 108020004414 DNA Proteins 0.000 description 19
- 239000000047 product Substances 0.000 description 8
- 108091034117 Oligonucleotide Proteins 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 238000012937 correction Methods 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 4
- 102000004506 Blood Proteins Human genes 0.000 description 3
- 108010017384 Blood Proteins Proteins 0.000 description 3
- 108020005202 Viral DNA Proteins 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 description 1
- 101150033828 NS1 gene Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000007859 qualitative PCR Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 229940016590 sarkosyl Drugs 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19752898A DE19752898A1 (de) | 1997-11-28 | 1997-11-28 | Verfahren zum Nachweis hoher Vierenkonzentrationen im Blutplasma und/oder Blutserum mittels der Polymerasekettenreaktion |
| DE19752898:8 | 1997-11-28 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006200332A Division JP2006335764A (ja) | 1997-11-28 | 2006-07-24 | 血漿生成物の製造方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11225797A true JPH11225797A (ja) | 1999-08-24 |
Family
ID=7850168
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10336431A Withdrawn JPH11225797A (ja) | 1997-11-28 | 1998-11-27 | 血漿および／または血清中の高ウイルス濃度をポリメラーゼ連鎖反応を用いて検出する方法 |
| JP2006200332A Pending JP2006335764A (ja) | 1997-11-28 | 2006-07-24 | 血漿生成物の製造方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006200332A Pending JP2006335764A (ja) | 1997-11-28 | 2006-07-24 | 血漿生成物の製造方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6183999B1 (enExample) |
| EP (1) | EP0922771B1 (enExample) |
| JP (2) | JPH11225797A (enExample) |
| KR (1) | KR100648843B1 (enExample) |
| AT (1) | ATE323184T1 (enExample) |
| CA (1) | CA2252013C (enExample) |
| DE (2) | DE19752898A1 (enExample) |
| ES (1) | ES2259196T3 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936442B2 (en) | 2001-06-28 | 2005-08-30 | Chiron Corporation | Diagnostic assays for parvovirus B19 |
| RU2644672C2 (ru) * | 2011-04-01 | 2018-02-13 | Оккам Байолэбс, Инк. | Способы и наборы для обнаружения бесклеточных специфических для патогенов нуклеиновых кислот |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT410218B (de) * | 1999-08-20 | 2003-03-25 | Baxter Ag | Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben |
| US7094541B2 (en) * | 2001-08-31 | 2006-08-22 | Gen-Probe Incorporated | Assay for detection of human parvovirus B19 nucleic acid |
| EP1521849A4 (en) * | 2001-10-12 | 2006-05-17 | Chiron Corp | HEPATITIS A-VIRUS NUCLEOTIDE SEQUENCES, RECOMBINANT PROTEINS AND THEIR USE |
| EP2251441B1 (en) * | 2004-02-10 | 2013-05-08 | F. Hoffmann-La Roche AG | Detection of parvovirus B19 |
| WO2006031608A2 (en) * | 2004-09-10 | 2006-03-23 | Focus Diagnostics, Inc. | Methods and compositions for detecting erythrovirus genotypes |
| AU2007233252C1 (en) | 2006-03-29 | 2013-05-23 | Boehringer Ingelheim Animal Health USA Inc. | Vaccine against Streptococci |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| CA2159044A1 (en) * | 1994-09-26 | 1996-03-27 | Falko-Guenter Falkner | Method of quantitating nucleic acids |
| AT406019B (de) * | 1995-05-08 | 2000-01-25 | Immuno Ag | Verfahren zur herstellung eines arzneimittels enthaltend ein oder mehrere plasmaderivate |
| AT403477B (de) * | 1996-04-30 | 1998-02-25 | Immuno Ag | Biologisches material frei von viralen und molekularen pathogenen und verfahren zur herstellung |
-
1997
- 1997-11-28 DE DE19752898A patent/DE19752898A1/de not_active Ceased
-
1998
- 1998-11-03 AT AT98120799T patent/ATE323184T1/de active
- 1998-11-03 ES ES98120799T patent/ES2259196T3/es not_active Expired - Lifetime
- 1998-11-03 DE DE59813486T patent/DE59813486D1/de not_active Expired - Lifetime
- 1998-11-03 EP EP98120799A patent/EP0922771B1/de not_active Revoked
- 1998-11-24 US US09/198,243 patent/US6183999B1/en not_active Expired - Lifetime
- 1998-11-26 CA CA2252013A patent/CA2252013C/en not_active Expired - Fee Related
- 1998-11-27 JP JP10336431A patent/JPH11225797A/ja not_active Withdrawn
- 1998-11-27 KR KR1019980051135A patent/KR100648843B1/ko not_active Expired - Fee Related
-
2006
- 2006-07-24 JP JP2006200332A patent/JP2006335764A/ja active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936442B2 (en) | 2001-06-28 | 2005-08-30 | Chiron Corporation | Diagnostic assays for parvovirus B19 |
| RU2644672C2 (ru) * | 2011-04-01 | 2018-02-13 | Оккам Байолэбс, Инк. | Способы и наборы для обнаружения бесклеточных специфических для патогенов нуклеиновых кислот |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE323184T1 (de) | 2006-04-15 |
| CA2252013C (en) | 2011-12-20 |
| US6183999B1 (en) | 2001-02-06 |
| JP2006335764A (ja) | 2006-12-14 |
| DE19752898A1 (de) | 1999-08-05 |
| EP0922771A2 (de) | 1999-06-16 |
| EP0922771B1 (de) | 2006-04-12 |
| KR19990045625A (ko) | 1999-06-25 |
| EP0922771A3 (de) | 2002-10-09 |
| DE59813486D1 (de) | 2006-05-24 |
| KR100648843B1 (ko) | 2007-03-02 |
| ES2259196T3 (es) | 2006-09-16 |
| CA2252013A1 (en) | 1999-05-28 |
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| A521 | Request for written amendment filed |
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| A761 | Written withdrawal of application |
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