JPH10509588A - E型肝炎ウイルス抗原およびその使用 - Google Patents
E型肝炎ウイルス抗原およびその使用Info
- Publication number
- JPH10509588A JPH10509588A JP8514117A JP51411796A JPH10509588A JP H10509588 A JPH10509588 A JP H10509588A JP 8514117 A JP8514117 A JP 8514117A JP 51411796 A JP51411796 A JP 51411796A JP H10509588 A JPH10509588 A JP H10509588A
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- JP
- Japan
- Prior art keywords
- hev
- polypeptide
- seq
- antigen
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
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- C—CHEMISTRY; METALLURGY
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/28011—Hepeviridae
- C12N2770/28111—Hepevirus, e.g. hepatitis E virus
- C12N2770/28122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/28011—Hepeviridae
- C12N2770/28111—Hepevirus, e.g. hepatitis E virus
- C12N2770/28123—Virus like particles [VLP]
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.E型肝炎ウイルス(HEV)オープンリーディングフレーム(ORF)2のカルボキ シ末端の549アミノ酸に由来する少なくとも1つのポリペプチドであって、ORF2 の全661アミノ酸を含まないポリペプチドを含有する、HEVポリペプチド組成物。 2.前記少なくとも1つのポリペプチドが、前記549アミノ酸のHEV ORF2ポリ ペプチドの約24カルボキシ末端アミノ酸までのカルボキシ末端欠失を含む、請求 項1に記載のポリペプチド組成物。 3.前記組成物が、配列番号15に示される配列または該配列と相同な配列を有 するポリペプチドを含有する、請求項1に記載のポリペプチド組成物。 4.前記組成物が、配列番号16に示される配列または該配列と相同な配列を有 するポリペプチドを含有する、請求項1に記載のポリペプチド組成物。 5.前記組成物が、配列番号25に示される配列または該配列と相同な配列を有 するポリペプチドを含有する、請求項1に記載のポリペプチド組成物。 6.前記組成物が、配列番号26に示される配列または該配列と相同な配列を有 するポリペプチドを含有する、請求項1に記載のポリペプチド組成物。 7.前記組成物が、配列番号27に示される配列または該配列と相同な配列を有 するポリペプチドを含有する、請求項1に記載のポリペプチド組成物。 8.前記組成物が、配列番号28に示される配列または該配列と相同な配列を有 するポリペプチドを含有する、請求項1に記載のポリペプチド組成物。 9.前記組成物が、配列番号25および配列番号27に示される配列、またはこれ らの配列と相同な配列を有する2つのポリペプチドを含有する、請求項1に記載 のポリペプチド組成物。 10.前記組成物が、配列番号26および配列番号28に示される配列、またはこ れらの配列と相同な配列を有する2つのポリペプチドを含有する、請求項1に記 載のポリペプチド組成物。 11.請求項1から10のいずれかに記載のポリペプチドをコードする、実質 的に単離された核酸配列。 12.請求項1から10のいずれかに記載のポリペプチドをコードする核酸配 列を含む、E型肝炎ウイルスポリペプチド抗原組成物を作製するための発現ベク ターであって、 該核酸配列は発現ベクター中に挿入され、該核酸配列は選択された宿主細胞に おいて転写を開始し得るプロモーターに作動可能に連結される、 発現ベクター。 13.請求項1から10のいずれかに記載のポリペプチドをコードする核酸配 列を含む、E型肝炎ウイルスポリペプチド抗原組成物を作製するための発現系で あって、 該核酸配列は発現ベクター中に挿入され、該核酸配列は選択された宿主細胞に おいて転写を開始し得るプロモーターに作動可能に連結され、そして該発現ベク ターは該宿主細胞内に保持される、 発現系。 14.前記発現ベクターがバキュロウイルス発現ベクターであり、そして前記 宿主細胞が昆虫細胞である、請求項13に記載の発現系。 15.E型肝炎ウイルス(HEV)ポリペプチド組成物であって、以下の工程: 請求項12に記載の発現ベクターを含む昆虫細胞を、前記核酸によりコードさ れるポリペプチドを発現させるに十分な条件下で培養する工程、 を包含するプロセスによって作製される、組成物。 16.前記組成物の少なくとも1つのポリペプチドが、配列番号15、配列番号 16、配列番号25、配列番号26、配列番号27、配列番号28、およびそれらと相同な 配列からなる群より選択されるアミノ酸配列を有する、請求項15に記載の組成 物。 17.E型肝炎ウイルス(HEV)ポリペプチド組成物であって、以下の工程: a)HEVキャプシドタンパク質の少なくとも549個のカルボキシ末端アミノ酸を 有するHEVキャプシド由来抗原を得る工程;および b)該抗原と、バキュロウイルスに感染した溶解物とを、該HEVキャプシド由来 抗原のカルボキシ末端配列を切断するに十分な条件下でインキュベートする工程 、を包含するプロセスによって作製される、組成物。 18.E型肝炎ウイルス(HEV)ポリペプチド組成物を作製する方法であって、 以下の工程: 請求項12に記載の発現ベクターを含む細胞を、前記核酸によりコードされる ポリペプチド配列を発現させるに十分な条件下で培養する工程、 を包含する、方法。 19.個体におけるE型肝炎ウイルス感染を検出する方法であって、以下の工 程: a)該個体から採取した血清サンプルを、請求項1から10のいずれかに記載 のE型肝炎ウイルス(HEV)ポリペプチド組成物と反応させる工程;および b)該組成物のポリペプチドを結合抗体の存在について検査する工程、 を包含する、方法。 20.前記HEVポリペプチド組成物のポリペプチドが固体支持体に結合し、前 記反応させる工程が、前記血清と該支持体とを接触させることを包含し、そして 前記検査する工程が、該支持体および結合抗体とレポーター標識抗ヒト抗体とを 反応させることを包含する、請求項19に記載の方法。 21.個体から採取した血清サンプルにおけるHEVに対する抗体の存在を確認 するためのキットであって、 表面に結合した抗原を有する固体支持体、ここで該表面に結合した抗原は請求 項1から10のいずれかに記載のHEVポリペプチド組成物のポリペプチドである 、を含む、キット。 22.個体をE型肝炎ウイルス(HEV)に対して免疫することに使用されるワク チン組成物であって、 薬理学的に受容可能なキャリアー中の請求項1から10のいずれかに記載のHE Vポリペプチド組成物、 を含有する、ワクチン組成物。 23.前記HEVポリペプチド組成物の少なくとも1つのポリペプチドが、配列 番号15、配列番号16、配列番号25、配列番号26、配列番号27、配列番号28、およ びそれらと相同な配列からなる群より選択されるアミノ酸配列を有する、請求項 22に記載のワクチン組成物。 24.HEVによる個体の感染を阻害する方法であって、 該個体に請求項21または22のいずれかに記載のワクチン組成物を、治療上 有効な量で投与する工程、 を包含する、方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US32795294A | 1994-10-24 | 1994-10-24 | |
US08/542,634 | 1995-10-13 | ||
US08/542,634 US6214970B1 (en) | 1988-06-17 | 1995-10-13 | Hepatitis E virus antigens and uses therefor |
US08/327,952 | 1995-10-13 | ||
PCT/US1995/013703 WO1996012807A2 (en) | 1994-10-24 | 1995-10-23 | Hepatitis e virus antigens and uses therefor |
Related Child Applications (1)
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JP2005354138A Division JP4237747B2 (ja) | 1994-10-24 | 2005-12-07 | E型肝炎ウイルス抗原およびその使用 |
Publications (1)
Publication Number | Publication Date |
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JPH10509588A true JPH10509588A (ja) | 1998-09-22 |
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JP8514117A Withdrawn JPH10509588A (ja) | 1994-10-24 | 1995-10-23 | E型肝炎ウイルス抗原およびその使用 |
JP2005354138A Expired - Fee Related JP4237747B2 (ja) | 1994-10-24 | 2005-12-07 | E型肝炎ウイルス抗原およびその使用 |
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JP2005354138A Expired - Fee Related JP4237747B2 (ja) | 1994-10-24 | 2005-12-07 | E型肝炎ウイルス抗原およびその使用 |
Country Status (8)
Country | Link |
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US (2) | US6214970B1 (ja) |
EP (1) | EP0787190B1 (ja) |
JP (2) | JPH10509588A (ja) |
AT (1) | ATE267256T1 (ja) |
AU (1) | AU4195696A (ja) |
DE (1) | DE69533067T2 (ja) |
ES (1) | ES2223054T3 (ja) |
WO (1) | WO1996012807A2 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2004525613A (ja) * | 2000-09-30 | 2004-08-26 | ヤン シェン タン イオン シィエン クゥオン ス | E型肝炎ウイルスのポリペプチド断片、それを含むワクチン組成物及び診断キット、並びにその使用 |
JP2005102689A (ja) * | 2003-09-08 | 2005-04-21 | Mitsubishi Kagaku Bio-Clinical Laboratories Inc | E型肝炎ウイルス様中空粒子 |
JP2019516745A (ja) * | 2016-05-25 | 2019-06-20 | インターベット インターナショナル ベー. フェー. | Hevワクチン |
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WO1994006913A2 (en) | 1992-09-18 | 1994-03-31 | The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services | Recombinant proteins of a pakistani strain of hepatitis e and their use in diagnostic methods and vaccines |
US6207416B1 (en) | 1992-09-18 | 2001-03-27 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant proteins of a Pakistani strain of hepatitis E and their use in diagnostic methods and vaccines |
US6787145B1 (en) | 1992-09-18 | 2004-09-07 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Recombinant proteins of a pakistani strain of hepatitis E and their use in diagnostic methods and vaccines |
US6054567A (en) * | 1997-04-11 | 2000-04-25 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant proteins of a pakistani strain of hepatitis E and their use in diagnostic methods and vaccines |
US6458562B1 (en) | 1998-04-09 | 2002-10-01 | The United States Of America As Represented By The Secretary Of Health And Human Services | Recombinant proteins of a Pakistani strain of hepatitis E and their use in diagnostic methods and vaccines |
CA2283538A1 (en) * | 1999-09-30 | 2001-03-30 | Mun Hon Ng | New hev antigenic peptide and methods |
US20040063188A1 (en) * | 2002-02-14 | 2004-04-01 | Novavax, Inc. | Kit for treating gastrointestinal tract |
JP5869885B2 (ja) * | 2009-02-27 | 2016-02-24 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | E型肝炎ウイルス様粒子を用いた多抗原送達系 |
US8906862B2 (en) | 2009-02-27 | 2014-12-09 | The Regents Of The University Of California | Multiple antigen delivery system using hepatitis E virus-like particle |
US8906863B2 (en) | 2009-02-27 | 2014-12-09 | The Regents Of The University Of California | Proteolysis-resistant capsid of chimeric hepatitis E virus as an oral delivery vector |
CN117083290A (zh) * | 2021-03-26 | 2023-11-17 | 港大科桥有限公司 | 戊型肝炎病毒样颗粒及其用途 |
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US6455492B1 (en) | 1988-06-17 | 2002-09-24 | Genelabs Technologies, Inc. | Hepatitis E virus vaccine and method |
US5885768A (en) | 1988-06-17 | 1999-03-23 | The United States Of America As Represented By The Department Of Health And Human Services | Hepatitis E virus peptide antigen and antibodies |
US5294548A (en) * | 1990-04-02 | 1994-03-15 | American Biogenetic Sciences, Inc | Recombianant Hepatitis a virus |
KR0178399B1 (ko) | 1990-04-05 | 1999-04-01 | 프랭크 쿵 | 장을 통해 전염되는 비a비b형 간염 바이러스 병원체 및 그것의 특유한 에피토프 |
WO1994006913A2 (en) | 1992-09-18 | 1994-03-31 | The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services | Recombinant proteins of a pakistani strain of hepatitis e and their use in diagnostic methods and vaccines |
SG95574A1 (en) | 1993-09-24 | 2003-04-23 | Macfarlane Burnet Ct For Medic | Immunoreactive antigens of hepatitia e virus |
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1995
- 1995-10-13 US US08/542,634 patent/US6214970B1/en not_active Expired - Fee Related
- 1995-10-23 EP EP95940546A patent/EP0787190B1/en not_active Expired - Lifetime
- 1995-10-23 DE DE69533067T patent/DE69533067T2/de not_active Expired - Lifetime
- 1995-10-23 AU AU41956/96A patent/AU4195696A/en not_active Abandoned
- 1995-10-23 ES ES95940546T patent/ES2223054T3/es not_active Expired - Lifetime
- 1995-10-23 AT AT95940546T patent/ATE267256T1/de not_active IP Right Cessation
- 1995-10-23 WO PCT/US1995/013703 patent/WO1996012807A2/en active IP Right Grant
- 1995-10-23 JP JP8514117A patent/JPH10509588A/ja not_active Withdrawn
-
2001
- 2001-05-29 US US09/769,066 patent/US20020107360A1/en not_active Abandoned
-
2005
- 2005-12-07 JP JP2005354138A patent/JP4237747B2/ja not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004525613A (ja) * | 2000-09-30 | 2004-08-26 | ヤン シェン タン イオン シィエン クゥオン ス | E型肝炎ウイルスのポリペプチド断片、それを含むワクチン組成物及び診断キット、並びにその使用 |
US8715695B2 (en) | 2000-09-30 | 2014-05-06 | Beijing Wantai Biological Pharmacy Enterprise Co., Ltd. | Polypeptide fragments of the hepatitis E virus, the vaccine composition comprising said fragments and the diagnostic kits |
JP2005102689A (ja) * | 2003-09-08 | 2005-04-21 | Mitsubishi Kagaku Bio-Clinical Laboratories Inc | E型肝炎ウイルス様中空粒子 |
JP2019516745A (ja) * | 2016-05-25 | 2019-06-20 | インターベット インターナショナル ベー. フェー. | Hevワクチン |
Also Published As
Publication number | Publication date |
---|---|
AU4195696A (en) | 1996-05-15 |
ES2223054T3 (es) | 2005-02-16 |
WO1996012807A2 (en) | 1996-05-02 |
JP2006141400A (ja) | 2006-06-08 |
DE69533067D1 (de) | 2004-06-24 |
US20020107360A1 (en) | 2002-08-08 |
US6214970B1 (en) | 2001-04-10 |
WO1996012807A3 (en) | 1996-05-30 |
JP4237747B2 (ja) | 2009-03-11 |
DE69533067T2 (de) | 2005-05-12 |
EP0787190A2 (en) | 1997-08-06 |
EP0787190B1 (en) | 2004-05-19 |
ATE267256T1 (de) | 2004-06-15 |
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