JPH10501128A - ヒアルロン酸の生産方法及び手段 - Google Patents
ヒアルロン酸の生産方法及び手段Info
- Publication number
- JPH10501128A JPH10501128A JP8500737A JP50073796A JPH10501128A JP H10501128 A JPH10501128 A JP H10501128A JP 8500737 A JP8500737 A JP 8500737A JP 50073796 A JP50073796 A JP 50073796A JP H10501128 A JPH10501128 A JP H10501128A
- Authority
- JP
- Japan
- Prior art keywords
- strain
- molecular weight
- million
- hyaluronic acid
- streptococcus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/885—Streptococcus
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.分子量が600万を超えるヒアルロン酸を生産するストレプトコッカスの超莢 膜株。 2.ストレップトコッカスA群又はC群であることを特徴とする請求項1に記載 の株。 3.パーコール勾配で1.03g/cm3以下の密度でバンドを形成することを特徴と する請求項1又は2に記載の株。 4.パーコール勾配で1.02-1.03g/cm3の範囲の密度でバンドを形成することを 特徴とする請求項3に記載の株。 5.高分子量ヒアルロン酸の生産方法であって、 (i)分子量が600万を超えるヒアルロン酸の生産能力を有するストレプトコ ッカスの超莢膜株を選別し、 (ii)リアクター中、温度30〜35℃で、実質的にせん断力のない撹拌条件下、 pHが5.6〜6.2の範囲の培養培地で前記株を培養し、 (iii)培養培地から段階(ii)で生成したHAを精製し、分子量が600万を超 えるHAを得る、 という各段階を含む方法。 6.前記株がストレプトコッカスA群又はC群であることを特徴とする請求項5 に記載の方法。 7.前記超莢膜株が変異誘発によって得られたことを特徴とする請求項5又は6 に記載の方法。 8.超莢膜細菌株により生産される分子量が600万を超えるヒアルロン酸。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9401806A SE9401806D0 (sv) | 1994-05-26 | 1994-05-26 | Method and means for the production of hyaluronic acid |
SE9401806-6 | 1994-05-26 | ||
PCT/SE1995/000585 WO1995033067A1 (en) | 1994-05-26 | 1995-05-24 | Method and means for the production of hyaluronic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH10501128A true JPH10501128A (ja) | 1998-02-03 |
JP3720361B2 JP3720361B2 (ja) | 2005-11-24 |
Family
ID=20394129
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50073796A Expired - Lifetime JP3720361B2 (ja) | 1994-05-26 | 1995-05-24 | ヒアルロン酸の生産方法及び手段 |
Country Status (13)
Country | Link |
---|---|
US (3) | US6090596A (ja) |
EP (1) | EP0760863B1 (ja) |
JP (1) | JP3720361B2 (ja) |
AT (1) | ATE221576T1 (ja) |
AU (1) | AU699404B2 (ja) |
CA (1) | CA2191197C (ja) |
DE (1) | DE69527626T2 (ja) |
DK (1) | DK0760863T3 (ja) |
ES (1) | ES2180639T3 (ja) |
NZ (1) | NZ287583A (ja) |
PT (1) | PT760863E (ja) |
SE (1) | SE9401806D0 (ja) |
WO (1) | WO1995033067A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019030292A (ja) * | 2017-08-04 | 2019-02-28 | 株式会社明治 | 乳酸菌の培養方法 |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE9401806D0 (sv) | 1994-05-26 | 1994-05-26 | Pharmacia Ab | Method and means for the production of hyaluronic acid |
US6455304B1 (en) | 1994-07-01 | 2002-09-24 | The Board Of Regents Of The University Of Oklahoma | Hyaluronate synthase gene and uses thereof |
US6951743B2 (en) | 1997-10-31 | 2005-10-04 | University Of Oklahoma Board Of Regents | Hyaluronan synthase genes and expression thereof in bacillus hosts |
US7091008B1 (en) | 1994-07-01 | 2006-08-15 | The Board Of Regents Of The University Of Oklahoma | Hyaluronan synthase genes and expression thereof in Bacillus hosts |
CN101113436B (zh) | 1997-10-31 | 2013-02-06 | 俄克拉何马大学董事会 | 透明质酸合酶基因及其应用 |
CN1322121C (zh) | 1997-10-31 | 2007-06-20 | 俄克拉何马大学董事会 | 透明质酸合酶基因及其应用 |
US7223571B2 (en) | 1998-04-02 | 2007-05-29 | The Board Of Regents Of The Universtiy Of Oklahoma | Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same |
US20060188966A1 (en) | 1998-04-02 | 2006-08-24 | Deangelis Paul L | Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same |
US6987023B2 (en) | 1998-04-02 | 2006-01-17 | The Board Of Regents Of The University Of Oklahoma | DNA encoding hyaluronan synthase from Pasteurella multocida and methods of use |
US7094581B2 (en) | 1998-10-26 | 2006-08-22 | The Board Of Regents Of The University Of Oklahoma | Hyaluronan synthases and methods of making and using same |
US6610666B1 (en) * | 1999-11-08 | 2003-08-26 | Bio-Hyos Ab | Hyaluronan product and process for manufacturing thereof |
CN1671400A (zh) * | 2002-07-03 | 2005-09-21 | 派瑞克科学公司 | 透明质酸组合物以及使用方法 |
JP4106361B2 (ja) * | 2002-08-19 | 2008-06-25 | コーロン インダストリーズ インク | ヒアルロン酸産生微生物菌株及び精製ヒアルロン酸の製造方法 |
KR100472007B1 (ko) * | 2002-08-19 | 2005-03-10 | 주식회사 코오롱 | 히아루론산 생산 균주 및 상기 균주를 이용한 히아루론산 생산방법 |
US6946551B2 (en) | 2003-03-12 | 2005-09-20 | New Life Resources, Llc | Preparation of hyaluronic acid from eggshell membrane |
US20080063677A1 (en) * | 2004-03-10 | 2008-03-13 | New Life Resources, Llc | Therapeutic, nutraceutical and cosmetic applications for eggshell membrane and processed eggshell membrane preparations |
US20040180025A1 (en) * | 2003-03-12 | 2004-09-16 | New Life Resources, Llc | Therapeutic, nutraceutical and cosmetic applications for eggshell membrane and processed eggshell membrane preparations |
US8580315B2 (en) * | 2004-03-10 | 2013-11-12 | Esm Technologies, Llc | Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations |
US7002007B2 (en) * | 2004-05-28 | 2006-02-21 | Calcigen Corporation | Production of high molecular weight hyaluronates |
US8802651B2 (en) | 2004-06-30 | 2014-08-12 | Abbott Medical Optics Inc. | Hyaluronic acid in the enhancement of lens regeneration |
PT1805312E (pt) * | 2004-09-23 | 2009-09-28 | Bayer Cropscience Ag | Processos e meios para produzir hialurano |
EP1640457A1 (de) * | 2004-09-23 | 2006-03-29 | Bayer CropScience GmbH | Verfahren und Mittel zur Herstellung von Hyaluronan |
EP1772052A1 (de) | 2005-10-05 | 2007-04-11 | Bayer CropScience GmbH | Verbesserte Verfahren und Mittel zur Herstellung von Hyaluronan |
WO2007089677A2 (en) * | 2006-01-27 | 2007-08-09 | University Of Massachusetts | Systems and methods for producing biofuels and related materials |
US7674781B2 (en) * | 2006-04-28 | 2010-03-09 | Heather Sheardown | Hyaluronic acid-retaining polymers |
KR101509139B1 (ko) * | 2006-11-23 | 2015-04-08 | 주식회사 엘지생명과학 | 히알루론산의 정제방법 |
US9579341B2 (en) | 2007-05-16 | 2017-02-28 | Johnson & Johnson Consumer Inc. | Preserved compositions containing hyaluronic acid or a pharmaceutically-acceptable salt thereof and related methods |
US8609634B2 (en) | 2007-05-16 | 2013-12-17 | Mcneil-Ppc, Inc. | Preserved compositions containing hyaluronic acid or a pharmaceutically-acceptable salt thereof and related methods |
KR101322227B1 (ko) | 2011-09-30 | 2013-10-28 | 일동제약주식회사 | 스트렙토코커스 디스갈락티애 id9103 균주 및 이를 이용한 히알루론산 생산 방법 |
JP2017112901A (ja) | 2015-12-24 | 2017-06-29 | キッコーマン株式会社 | 高分子ヒアルロン酸の製造方法 |
US11129862B2 (en) | 2017-08-30 | 2021-09-28 | Johnson & Johnson Consumer Inc. | Ophthalmic compositions |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62501471A (ja) * | 1985-01-18 | 1987-06-18 | バイオ−テクノロジ−・ジエネラル・コ−ポレイシヨン | 連鎖球菌の発酵による高分子量ヒアルロン酸ナトリウムの製造方法 |
JPS6467196A (en) * | 1987-09-08 | 1989-03-13 | Yakult Honsha Kk | Production of hyaluronic acid and strain used therefor |
JPH02167301A (ja) * | 1988-12-21 | 1990-06-27 | Riken Vitamin Co Ltd | ヒアルロン酸ビーズの製造法 |
JPH03503905A (ja) * | 1989-02-08 | 1991-08-29 | バイオマトリツクス・インコーポレイテツド | 架橋ヒアルロン酸ゲル、その使用およびその製造方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4141973A (en) * | 1975-10-17 | 1979-02-27 | Biotrics, Inc. | Ultrapure hyaluronic acid and the use thereof |
US4517295A (en) * | 1983-02-18 | 1985-05-14 | Diagnostic, Inc. | Hyaluronic acid from bacterial culture |
NO161573C (no) * | 1983-11-25 | 1989-08-30 | Miles Inc | Fremgangsmaate til fremstilling av hyaluronsyre. |
CA1328841C (en) | 1987-06-12 | 1994-04-26 | Karen K. Brown | Method for the microbiological production of non-antigenic hyaluronic acid |
US5015577A (en) * | 1989-08-29 | 1991-05-14 | Board Of Regents, The University Of Texas System | DNA encoding hyaluronate synthase |
GB9024223D0 (en) * | 1990-11-07 | 1990-12-19 | Fermentech Ltd | Production of hyaluronic acid |
US5681825A (en) | 1993-03-15 | 1997-10-28 | Lindqvist; Bengt | Surgical method |
SE9401806D0 (sv) * | 1994-05-26 | 1994-05-26 | Pharmacia Ab | Method and means for the production of hyaluronic acid |
-
1994
- 1994-05-26 SE SE9401806A patent/SE9401806D0/xx unknown
-
1995
- 1995-05-24 NZ NZ287583A patent/NZ287583A/en unknown
- 1995-05-24 AU AU26335/95A patent/AU699404B2/en not_active Expired
- 1995-05-24 ES ES95921198T patent/ES2180639T3/es not_active Expired - Lifetime
- 1995-05-24 US US08/737,408 patent/US6090596A/en not_active Expired - Lifetime
- 1995-05-24 CA CA002191197A patent/CA2191197C/en not_active Expired - Lifetime
- 1995-05-24 DE DE69527626T patent/DE69527626T2/de not_active Expired - Lifetime
- 1995-05-24 JP JP50073796A patent/JP3720361B2/ja not_active Expired - Lifetime
- 1995-05-24 EP EP95921198A patent/EP0760863B1/en not_active Expired - Lifetime
- 1995-05-24 DK DK95921198T patent/DK0760863T3/da active
- 1995-05-24 AT AT95921198T patent/ATE221576T1/de not_active IP Right Cessation
- 1995-05-24 WO PCT/SE1995/000585 patent/WO1995033067A1/en active IP Right Grant
- 1995-05-24 PT PT95921198T patent/PT760863E/pt unknown
-
2000
- 2000-02-01 US US09/496,149 patent/US6537795B1/en not_active Expired - Lifetime
-
2003
- 2003-01-29 US US10/353,891 patent/US7323329B2/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62501471A (ja) * | 1985-01-18 | 1987-06-18 | バイオ−テクノロジ−・ジエネラル・コ−ポレイシヨン | 連鎖球菌の発酵による高分子量ヒアルロン酸ナトリウムの製造方法 |
JPS6467196A (en) * | 1987-09-08 | 1989-03-13 | Yakult Honsha Kk | Production of hyaluronic acid and strain used therefor |
JPH02167301A (ja) * | 1988-12-21 | 1990-06-27 | Riken Vitamin Co Ltd | ヒアルロン酸ビーズの製造法 |
JPH03503905A (ja) * | 1989-02-08 | 1991-08-29 | バイオマトリツクス・インコーポレイテツド | 架橋ヒアルロン酸ゲル、その使用およびその製造方法 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019030292A (ja) * | 2017-08-04 | 2019-02-28 | 株式会社明治 | 乳酸菌の培養方法 |
Also Published As
Publication number | Publication date |
---|---|
NZ287583A (en) | 1998-02-26 |
EP0760863A1 (en) | 1997-03-12 |
AU699404B2 (en) | 1998-12-03 |
US20030134393A1 (en) | 2003-07-17 |
WO1995033067A1 (en) | 1995-12-07 |
EP0760863B1 (en) | 2002-07-31 |
DE69527626D1 (de) | 2002-09-05 |
DE69527626T2 (de) | 2003-02-13 |
US6537795B1 (en) | 2003-03-25 |
CA2191197C (en) | 2002-01-15 |
DK0760863T3 (da) | 2002-11-25 |
PT760863E (pt) | 2002-11-29 |
US6090596A (en) | 2000-07-18 |
AU2633595A (en) | 1995-12-21 |
ES2180639T3 (es) | 2003-02-16 |
JP3720361B2 (ja) | 2005-11-24 |
SE9401806D0 (sv) | 1994-05-26 |
CA2191197A1 (en) | 1995-12-07 |
US7323329B2 (en) | 2008-01-29 |
ATE221576T1 (de) | 2002-08-15 |
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