JPH10226633A - Cosmetic composition containing extract of chestnut bur - Google Patents
Cosmetic composition containing extract of chestnut burInfo
- Publication number
- JPH10226633A JPH10226633A JP10029417A JP2941798A JPH10226633A JP H10226633 A JPH10226633 A JP H10226633A JP 10029417 A JP10029417 A JP 10029417A JP 2941798 A JP2941798 A JP 2941798A JP H10226633 A JPH10226633 A JP H10226633A
- Authority
- JP
- Japan
- Prior art keywords
- chestnut
- cosmetic composition
- extract
- water
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
Abstract
Description
【0001】本発明は、栗の木属植物の実である栗の心
皮(栗皮)から分離、精製する工程により皮膚改善効果
を有する抽出物を含有する化粧料組成物に関するもので
ある。[0001] The present invention relates to a cosmetic composition containing an extract having a skin-improving effect by a process of separating and purifying chestnut rind (chestnut skin), which is a fruit of a chestnut tree genus plant.
【0002】一般的に化粧料は、肌を清浄にし、肌を美
化し、老化を防ぐ役割をする。その主な作用としては、
肌に水分を供給し、および肌から水分が過渡に蒸発する
ことを防ぐ補湿効果をはじめとして、有害環境により人
体内部に生成される反応性の高い活性酸素や遊離基、過
酸化物により生体成分(蛋白質、核酸、細胞膜脂質な
ど)が酸化、変性されて皮膚老化の主な原因として働い
ていることを阻害、除去する遊離基の消去作用、および
ドーパ(DOPA)自動酸化に対する阻害作用などが挙げられ
る。[0002] In general, cosmetics serve to cleanse the skin, beautify the skin and prevent aging. Its main functions are:
In addition to the moisturizing effect that supplies moisture to the skin and prevents the water from evaporating from the skin transiently, highly reactive active oxygen, free radicals, and peroxides generated inside the human body due to harmful environments Inhibits the components (proteins, nucleic acids, cell membrane lipids, etc.) from being oxidized and denatured to work as a major cause of skin aging, eliminating free radicals to remove them, and inhibiting dopa (DOPA) autoxidation. No.
【0003】最近各種の植物抽出物が化粧料として研
究、開発 されて使用されているが、これらは前記のよ
うな補湿効果または美白効果などの唯一つのみの効能を
主にもつ。[0003] Recently, various plant extracts have been studied, developed and used as cosmetics, but they mainly have only one effect such as the above-mentioned moisturizing effect or whitening effect.
【0004】本発明者は、このような現実を勘案し、よ
り優れる化粧料を探すために、天然植物を対象に化粧料
としての有効性を調査した結果、栗皮抽出物は安全性が
優れ、皮膚改善および美白効果に有効な性質をもってい
ることが分かった。[0004] In view of such a reality, the present inventor investigated the effectiveness as a cosmetic for natural plants in order to search for a better cosmetic. As a result, the chestnut bark extract has excellent safety. It was found to have properties effective for skin improvement and whitening effect.
【0005】栗の木属には、栗の木(Castanea crenata
S. et Z., Castanea mo1lissima Bl.,Castanea bulgari
s)、薬栗の木(Castanea Bungeana Bl)、桑の木(Castane
a crenatafor. multicarpa(Uyeki)Chung)があり、これ
らは、落葉闊葉樹の喬木で、垂直的に標高100〜1,
100m、水平的には、大韓民国全域に養生しており、
日本、満州等にも分布する。葉は互い違いに出た楕円形
または長楕円状の被針形で、元は丸みを帯びるかまたは
ハート型であり、先は尖っていてぎざぎざがある。花
は、雌雄同株であって、5〜6月に咲く。果実は、堅果
で、卵形または里芋形であり、外皮は、偏球形で、外面
にとげが多く、9〜10月に実る。Chestnut trees include the chestnut tree (Castanea crenata)
S. et Z., Castanea mo1lissima Bl., Castanea bulgari
s), medicine chestnut tree (Castanea Bungeana Bl), mulberry tree (Castane
acarenatafor.multicarpa (Uyeki) Chung), which are deciduous broad-leaved trees with a vertical height of 100 to 1,
100m, horizontally, it has been cured throughout Korea.
It is also distributed in Japan and Manchuria. The leaves are staggered elliptical or oblong needle-shaped, originally rounded or heart-shaped, pointed and jagged. The flowers are hermaphroditic and bloom from May to June. The fruits are nut-shaped, ovoid or taro-shaped, and the hulls are oblate, spiny on the outer surface and grow in September-October.
【0006】栗皮は栗の果実を包んでいる濃い褐色の心
皮で、栗の果実から分離して天日に干した後、使用す
る。本発明は、栗皮抽出物を含有する皮膚改善の化粧料
組成物を提供することにその目的がある。Chestnut skin is a dark brown carpel that wraps the chestnut fruit and is used after being separated from the chestnut fruit and dried on the sun. An object of the present invention is to provide a cosmetic composition for improving skin containing a chestnut bark extract.
【0007】本発明をもっと詳細に説明すれば次のとお
りである。本発明は栗皮抽出物を含有する化粧料組成物
に関するものである。本発明の栗皮抽出物は化粧料の乾
燥重量に対して0.001〜10重量%、望ましくは0.
01〜5重量%の含量で含有される。[0007] The present invention will be described in more detail as follows. The present invention relates to a cosmetic composition containing a chestnut bark extract. The chestnut bark extract of the present invention is 0.001 to 10% by weight, preferably 0.1 to 10% by weight based on the dry weight of the cosmetic.
It is contained at a content of 01 to 5% by weight.
【0008】本発明は、野生のまたは栽培された栗の木
の実である栗の心皮に、水、またはアセトン、エチルア
セテート、ジエチルエーテル、ベンゼン、クロロホル
ム、ヘキサン、炭素数1〜4個の低級アルコールなどの
有機溶媒、あるいは各々の有機溶媒と水との混合物を溶
媒として得た抽出物を化粧料、即ち柔軟化粧水(スキ
ン)、栄養化粧水(ローション)、栄養クリーム、マッ
サージクリーム、エセンス、パックなどに添加すること
である。[0008] The present invention relates to a method for producing water or acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, a lower alcohol having 1 to 4 carbon atoms on chestnut carp, which is a nut of a wild or cultivated chestnut tree. Extracts obtained by using an organic solvent such as an organic solvent or a mixture of each organic solvent and water as a solvent are cosmetics, that is, a softening lotion (skin), a nourishing lotion (lotion), a nourishing cream, a massage cream, an essence, and a pack. And so on.
【0009】本発明の栗皮抽出物の製法をさらに詳しく
説明すると、栗の心皮(栗皮)をきれいな水で洗浄、乾
燥した後、微細に粉末化する。この粉末化した栗皮の乾
燥重量に対して抽出溶媒として水、またはアセトン、エ
チルアセテート、ジエチルエーテル、ベンゼン、クロロ
ホルム、ヘキサン、炭素数1〜4個の低級アルコール、
あるいは各々と水との混合物を加える。この抽出溶媒
は、栗皮粉末の乾燥重量に対して、1〜15倍の量で用
いられるとよい。The method of producing the chestnut skin extract of the present invention will be described in more detail. Chestnut carpel (chestnut skin) is washed with clean water, dried, and finely powdered. Water or acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, a lower alcohol having 1 to 4 carbon atoms as an extraction solvent with respect to the dry weight of the powdered chestnut skin,
Alternatively, a mixture of each and water is added. This extraction solvent is preferably used in an amount of 1 to 15 times the dry weight of the chestnut powder.
【0010】抽出方法としては、抽出器に冷却コンデン
サーを備え、この冷却コンデンサーにより有効成分が蒸
発されることを防ぐ状態で、50〜95℃で1〜20時
間にわたって加熱して抽出し、あるいは5〜40℃で1
〜15日間にわたって沈積させて有効成分を抽出する。
このように抽出された栗皮抽出物を、冷却コンデンサー
を備えた蒸留装置を利用して、完全に減圧濃縮し本発明
の栗皮抽出物を製造する。As an extraction method, the extractor is provided with a cooling condenser, and the extract is heated and heated at 50 to 95 ° C. for 1 to 20 hours while preventing the active ingredient from being evaporated by the cooling condenser. 1 at ~ 40 ° C
Allow to sediment for ~ 15 days to extract the active ingredient.
The chestnut skin extract thus extracted is completely concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser to produce the chestnut skin extract of the present invention.
【0011】[0011]
【実施例】以下の実施例及び実験例は本発明の内容をよ
り具体的に説明するが、本発明の内容がこれにより限定
されることはない。The following examples and experimental examples illustrate the contents of the present invention more specifically, but do not limit the contents of the present invention.
【0012】[0012]
【実施例1】精製水で洗浄、乾燥した後粉末化した1kg
の栗皮粉末を5Lの水に入れ、冷却コンデンサーを備え
た抽出器で、5時間にわたって加熱して抽出した。これ
を300メッシュの濾過布で濾過し、4〜15℃で、7
日間放置して低温熟成させてから、ワットマン2番(Wh
atman No.2)濾過紙で濾過した。この濾液を60℃で回
転減圧蒸発機(エバポレータ)を利用して乾燥して1
7.5g(乾燥重量)を得た。Example 1 1 kg of powder after washing and drying with purified water
Was placed in 5 L of water and extracted by heating for 5 hours with an extractor equipped with a cooling condenser. This was filtered through a 300 mesh filter cloth,
After leaving it for a few days and aging at low temperature, Whatman No. 2 (Wh
atman No. 2) The mixture was filtered with filter paper. The filtrate was dried at 60 ° C. using a rotary vacuum evaporator (evaporator) and dried.
7.5 g (dry weight) were obtained.
【0013】[0013]
【実施例2】精製水で洗浄、乾燥した後粉末化した1kg
の栗皮粉末を10%エタノール(5L)に入れ、冷却コン
デンサーを備えた抽出器で、3時間にわたって加熱して
抽出した。これを300メッシュの濾過布で濾過し、4
〜15℃で、7日間放置して低温熟成させてから、ワッ
トマン2番濾過紙で濾過した。この濾液を60℃でエバ
ポレータを利用して乾燥して21.8g(乾燥重量)を
得た。Example 2 1 kg of powder after washing and drying with purified water
Chestnut powder was placed in 10% ethanol (5 L) and extracted by heating for 3 hours with an extractor equipped with a cooling condenser. This was filtered through a 300-mesh filter cloth,
The mixture was allowed to stand at -15 ° C for 7 days to be aged at a low temperature, and then filtered through Whatman No. 2 filter paper. The filtrate was dried at 60 ° C. using an evaporator to obtain 21.8 g (dry weight).
【0014】[0014]
【実施例3〜11】実施例2と同様の方法で抽出してそ
の結果を次の表1に示す。Embodiments 3 to 11 Extraction is performed in the same manner as in Embodiment 2, and the results are shown in Table 1 below.
【0015】[0015]
【表1】 [Table 1]
【0016】[0016]
【実施例12】精製水で洗浄、乾燥した後粉末化した1
kgの栗皮粉末を5Lの水に入れ、15〜40℃で、3日
間にわたって抽出した。これを300メッシュの濾過布
で濾過し、4〜15℃で、7日間放置して低温熟成させ
てから、ワットマン2番濾過紙で濾過した。この抽出物
を冷却コンデンサーを備えた蒸留装置を利用して60℃
で、減圧濃縮して40.5g(乾燥重量)を得た。Example 12 Powder 1 after washing and drying with purified water
kg of chestnut skin powder was placed in 5 L of water and extracted at 15-40 ° C. for 3 days. This was filtered through a 300-mesh filter cloth, allowed to stand at 4 to 15 ° C. for 7 days to be aged at a low temperature, and then filtered through Whatman No. 2 filter paper. This extract was collected at 60 ° C. using a distillation apparatus equipped with a cooling condenser.
And concentrated under reduced pressure to obtain 40.5 g (dry weight).
【0017】[0017]
【実施例13】精製水で洗浄、乾燥した後粉末化した1
kgの栗皮粉末を5Lの10%エタノールに入れ、15〜
40℃で、3日間にわたって抽出した。これを300メ
ッシュの濾過布で濾過し、4〜15℃で、7日間放置し
て低温熟成させてから、ワットマン2番濾過紙で濾過し
た。この濾液を60℃でエバポレータを利用して乾燥し
て38.9g(乾燥重量)を得た。Example 13 Powder 1 after washing and drying with purified water
kg chestnut skin powder in 5 L of 10% ethanol
Extracted at 40 ° C. for 3 days. This was filtered through a 300-mesh filter cloth, allowed to stand at 4 to 15 ° C. for 7 days to be aged at a low temperature, and then filtered through Whatman No. 2 filter paper. The filtrate was dried at 60 ° C. using an evaporator to obtain 38.9 g (dry weight).
【0018】[0018]
【実施例14〜34】実施例13と同様の方法で抽出し
てその結果を次の表2に示す。Embodiments 14 to 34 The extraction is performed in the same manner as in Embodiment 13, and the results are shown in Table 2 below.
【0019】[0019]
【表2】 [Table 2]
【0020】[0020]
【実験例1】前記栗皮抽出物によるチロシナーゼ活性に
対する阻害効果を測定した。 1)実験方法 実施例1〜34で製造した栗皮の抽出物について、チロ
シナーゼ活性阻害効果を測定した。チロシナーゼは、き
のこから分離及び精製したもので、シグマ(Sigma)社よ
り購入して使用した。基質であるチロシンを、0.05
M燐酸ナトリウム緩衝溶液(pH 6.8)に溶かし、0.1mg/
m1チロシン溶液にして使用した。本発明の各種の抽出物
を低適性濃度とし、0.5m1のチロシン溶液を混合した
後37℃恒温器で10分間放置し、 その後300U/ml
チロシナーゼ0.5mlを入れ、37℃で10分間反応さ
せた。この時の対照群は、チロシン溶液を0.5ml入れ
たものである。所定時間の反応後、試験管を氷の上に置
いて急冷し、反応を停止させて分光光度計で波長475
nmでの吸光度を測定した。Experimental Example 1 The inhibitory effect of the chestnut extract on tyrosinase activity was measured. 1) Experimental method The tyrosinase activity inhibitory effect of the chestnut bark extract prepared in Examples 1 to 34 was measured. Tyrosinase was isolated and purified from mushrooms, and was used after being purchased from Sigma. Substrate tyrosine was added to 0.05
M sodium phosphate buffer solution (pH 6.8), 0.1 mg /
It was used as an m1 tyrosine solution. Each extract of the present invention was adjusted to a low suitable concentration, mixed with a 0.5 ml tyrosine solution, allowed to stand in a thermostat at 37 ° C. for 10 minutes, and then 300 U / ml.
0.5 ml of tyrosinase was added and reacted at 37 ° C. for 10 minutes. At this time, the control group contained 0.5 ml of the tyrosine solution. After the reaction for a predetermined time, the test tube was quenched by placing it on ice, and the reaction was stopped.
The absorbance at nm was measured.
【0021】栗皮抽出物によるチロシナーゼ活性に対す
る阻害効果は次の公式により求めた。 チロシナーゼの活性阻害率(%)=100-(100 X 各抽出
物の反応後の吸光度/対照群の反応後の吸光度) 2)実験結果 吸光度を測定してチロシナーゼ活性に対する阻害効果を
測定した結果、実施例20の栗皮抽出物によるチロシナ
ーゼに対するIC50濃度は80μg/mlであった。The inhibitory effect of the chestnut bark extract on tyrosinase activity was determined by the following formula. Tyrosinase activity inhibition rate (%) = 100− (100 × absorbance after reaction of each extract / absorbance after reaction of control group) 2) Experimental results As a result of measuring the absorbance, the inhibitory effect on tyrosinase activity was measured. The IC50 concentration against tyrosinase by the chestnut bark extract of Example 20 was 80 μg / ml.
【0022】実施例1〜34の物質を用いた実験結果は
次の表3に示す。The results of experiments using the substances of Examples 1 to 34 are shown in Table 3 below.
【0023】[0023]
【表3】 [Table 3]
【0024】[0024]
【実験例2】前記栗皮抽出物によるドーパ(DOPA)の自動
酸化に対する阻害作用を測定した。 1)実験方法 次の方法で阻害能を測定した。Experimental Example 2 The inhibitory effect of the chestnut extract on autoxidation of dopa (DOPA) was measured. 1) Experimental method The inhibitory ability was measured by the following method.
【0025】緩衝溶液としてナトリウム−燐酸塩(0.05
M、pH6.8)およびドーパ(500μM)を準備し、全体の
反応容積を2mlとして37℃で48時間反応させてか
ら、475nmで吸光度を測定した。この時に栗皮抽出物
を、この緩衝溶液に溶かして添加した。As a buffer solution, sodium-phosphate (0.05
M, pH 6.8) and dopa (500 μM) were prepared, reacted at 37 ° C. for 48 hours in a total reaction volume of 2 ml, and the absorbance was measured at 475 nm. At this time, the chestnut bark extract was dissolved in this buffer solution and added.
【0026】各栗皮抽出物によるドーパ(DOPA)の自動酸
化に対する阻害効果は次の公式により求める。 ドーパの自動酸化阻害率(%)=100-(100 X 各抽出物
の反応後の吸光度/対照群の反応後の吸光度) 2)実験結果 吸光度を測定してドーパ(DOPA)の自動酸化に対する阻害
効果を測定した結果、実施例20の栗皮抽出物によるド
ーパの自動酸化に対するIC50濃度は746.67μg/ml
であった。The inhibitory effect of each chestnut extract on the autoxidation of dopa (DOPA) is determined by the following formula. Dopa autoxidation inhibition rate (%) = 100-(100 x absorbance after reaction of each extract / absorbance after reaction of control group) 2) Experimental results Inhibition of dopa (DOPA) against autoxidation by measuring absorbance As a result of measuring the effect, the IC50 concentration for autoxidation of dopa by the chestnut bark extract of Example 20 was 746.67 μg / ml.
Met.
【0027】実施例1〜34の物質を用いた実験結果は
次の表4に示す。The results of experiments using the substances of Examples 1 to 34 are shown in Table 4 below.
【0028】[0028]
【表4】 [Table 4]
【0029】[0029]
【実験例3】前記栗皮抽出物による遊離基の消去作用を
測定した。 1)実験方法 次の方法で遊離基の消去作用を検定した。Experimental Example 3 The free radical scavenging effect of the chestnut extract was measured. 1) Experimental method The scavenging effect of free radicals was examined by the following method.
【0030】60μMのDPPH(1,1-diphenyl-2-picrylhy
drazyl)溶液2mlに適性濃度の栗皮抽出物1mlを加えた
後、37℃で30分間反応させてから、520nmで吸光
度を測定した。この時に各栗皮抽出物を、DMSO(Dimethy
l sulfoxide)に溶かして添加した。 2)実験結果 吸光度を測定して遊離基の消去作用を測定した結果、実
施例20の方法を利用して得た栗皮抽出物による遊離基
の消去作用のIC50濃度は8μg/mlであった。この値は、
強力な遊離基の消去剤である没食子酸のIC50(1.5μg/m
l)に匹敵するような活性を示し、強力な合成抗酸化剤で
あるBHT(IC50:37μg/ml)やアスコルビン酸(IC50:2
9μg/ml)よりさらに高い活性を示した。60 μM of DPPH (1,1-diphenyl-2-picrylhy
After adding 1 ml of an appropriate concentration of chestnut skin extract to 2 ml of the drazyl solution, the mixture was reacted at 37 ° C. for 30 minutes, and the absorbance was measured at 520 nm. At this time, each chestnut extract was added to DMSO (Dimethy
l sulfoxide). 2) Experimental results The absorbance was measured to determine the free radical scavenging effect. As a result, the IC50 concentration of the free radical scavenging effect of the chestnut bark extract obtained using the method of Example 20 was 8 μg / ml. . This value is
IC50 (1.5 μg / m2) of gallic acid, a strong free radical scavenger
l) It shows activity comparable to that of l) and is a strong synthetic antioxidant such as BHT (IC50: 37 µg / ml) or ascorbic acid (IC50: 2).
9 μg / ml).
【0031】実施例1〜34の方法でよって得た栗皮抽
出物の実験結果は次の表5に示す。The experimental results of the chestnut bark extract obtained by the methods of Examples 1 to 34 are shown in Table 5 below.
【0032】[0032]
【表5】 [Table 5]
【0033】[0033]
【実験例4】前記栗皮抽出物による補湿効果を測定し
た。 1)実験方法 後述する処方例3の方法によって製造した栗皮の抽出物
を含有した栄養クリームの組成物と、含有しない組成物
とを、各々腕の上膊や手などに一日3回ずつ一週間にわ
たって塗布し、硬皮水分損失量測定機チワメーターTM21
0(C+K electronic GmbH, Germany)を利用して硬皮水分
損失量(TEWL:Transepidermal Water Loss)を時間帯別
に測定し、時間変化にともなうTEWL値の変化量(△TEWL)
により、保湿効果を測定した。この時の実験条件は、温
度25℃、相対湿度45%の恒温恒湿室であった。皮膚
の水分量測定機コルニオメーターCM 820 PC(C+K electr
onicGmbH, Germany)を利用した表皮水分量の変化に対す
る電気伝度度の変化を数値とした値で、0から150ま
で皮膚の水分量を数値化して補湿効果を測定した。 2)実験結果 栗皮抽出物を含有した栄養クリームの組成物が、栗皮抽
出物を含有しない組成物より、△TEWL数値を1.6±0.
4(g/hm2) 低く示した。表皮水分量は、栗皮抽出物を含
有した組成物が含有しない組成物より平均、10以上の
高い数値を表し、栗皮抽出物が保湿効果を持つことを確
認できた。Experimental Example 4 The moisturizing effect of the chestnut bark extract was measured. 1) Experimental method The composition of a nutritional cream containing the extract of chestnut bark produced by the method of Formulation Example 3 to be described later, and the composition not containing the extract are respectively applied to the upper arm or hand of an arm three times a day. Applied for one week, scalp water loss meter Chiwa Meter TM21
Using 0 (C + K electronic GmbH, Germany), the transepidermal water loss (TEWL) is measured for each time zone, and the change in TEWL value over time (△ TEWL)
, The moisturizing effect was measured. The experimental conditions at this time were a constant temperature and humidity room at a temperature of 25 ° C. and a relative humidity of 45%. Skin moisture meter Corniometer CM 820 PC (C + K electr
(onicGmbH, Germany) using the value of the change in electrical conductivity with respect to the change in epidermal water content, and the moisture content of the skin was quantified from 0 to 150 to measure the moisturizing effect. 2) Experimental results The nutritional cream composition containing the chestnut bark extract had a ΔTEWL value of 1.6 ± 0.2 compared to the composition not containing the chestnut bark extract.
4 (g / hm 2 ). The water content of the epidermis was higher than the composition not containing the composition containing the chestnut bark extract on average by 10 or more, confirming that the chestnut bark extract has a moisturizing effect.
【0034】[0034]
【比較例1】精製水で洗浄、乾燥した五倍子1kgを用い
て、実施例23と同様の方法で抽出して乾燥物を得た。COMPARATIVE EXAMPLE 1 A dried product was obtained by extracting in the same manner as in Example 23, using 1 kg of a quintet washed and dried with purified water.
【0035】[0035]
【比較例2】精製水で洗浄、乾燥した茄子1kgを用い
て、実施例23と同様の方法で抽出して乾燥物を得た。COMPARATIVE EXAMPLE 2 1 kg of eggplant washed and dried with purified water was extracted in the same manner as in Example 23 to obtain a dried product.
【0036】[0036]
【比較例3】精製水で洗浄、乾燥した地楡1kgを用い
て、実施例23と同様の方法で抽出して乾燥物を得た。Comparative Example 3 Using 1 kg of ground water washed and dried with purified water, extraction was performed in the same manner as in Example 23 to obtain a dried product.
【0037】[0037]
【比較例4】精製水で洗浄、乾燥したサンシュユ1kgを
用いて、実施例23と同様の方法で抽出して乾燥物を得
た。Comparative Example 4 A dried product was obtained by extracting in the same manner as in Example 23, using 1 kg of Sanshuyu washed and dried with purified water.
【0038】[0038]
【実験例5】栗皮抽出物と前記比較例1〜4により製造
した抽出物とで、濃度によるチロシナーゼ活性の阻害効
果、ドーパ(DOPA)の自動酸化に対する阻害効果、遊離基
の消去作用を実験例1−3と同様の方法で測定した。[Experimental example 5] The inhibitory effect of tyrosinase activity, the inhibitory effect of dopa (DOPA) on autoxidation, and the elimination of free radicals were tested by using the chestnut bark extract and the extracts prepared in Comparative Examples 1-4. It measured by the method similar to Example 1-3.
【0039】栗皮抽出物の含有されている生物活性成分
を、タンニン成分が多く含まれている五倍子、茄子、地
楡、サンシュユの抽出物と比較した。実験結果は次の表
6に示す。The biologically active components contained in the chestnut bark extract were compared with the extract of pentaploid, eggplant, jiyu and sanshuyu, which contained a large amount of tannin component. The experimental results are shown in Table 6 below.
【0040】[0040]
【表6】 [Table 6]
【0041】栗皮抽出物は、他のタンニン成分を含有す
る植物抽出物に比べてチロシナーゼ活性の阻害、ドーパ
(DOPA)の自動酸化阻害および遊離基の消去作用が全て高
い活性を示したので、化粧料として等しい効能を有して
いる。The chestnut bark extract inhibits tyrosinase activity and inhibits dopa as compared to other plant extracts containing tannin components.
(DOPA) exhibited high activity in all of the autoxidation inhibition and free radical scavenging effects, and thus have the same efficacy as cosmetics.
【0042】[0042]
【処方例1】栗皮抽出物を含有した化粧料の中、柔軟化
粧水(スキン)の処方例は次の表7である。ここで、栗
皮抽出物は実施例20のものをいう。Formulation Example 1 Among the cosmetics containing the chestnut extract, a formulation example of a soft lotion (skin) is shown in Table 7 below. Here, the chestnut skin extract refers to that of Example 20.
【0043】[0043]
【表7】 [Table 7]
【0044】[0044]
【処方例2】栗皮抽出物を含有した化粧料の中、栄養化
粧水(ローション)の処方例は次の表8である。ここ
で、栗皮抽出物は実施例20のものをいう。[Formulation Example 2] Among the cosmetics containing the chestnut bark extract, the formulation examples of the nutrition lotion (lotion) are shown in Table 8 below. Here, the chestnut skin extract refers to that of Example 20.
【0045】[0045]
【表8】 [Table 8]
【0046】[0046]
【処方例3】栗皮抽出物を含有した化粧料の中、栄養ク
リームの処方例は次の表9である。ここで、栗皮抽出物
は実施例20のものをいう。[Formulation Example 3] Among the cosmetics containing the chestnut extract, a formulation example of the nutritional cream is shown in Table 9 below. Here, the chestnut skin extract refers to that of Example 20.
【0047】[0047]
【表9】 [Table 9]
【0048】[0048]
【処方例4】栗皮抽出物を含有した化粧料の中、マッサ
ージクリームの処方例は次の表10である。ここで、栗
皮抽出物は実施例20のものをいう。[Formulation Example 4] Formulation examples of massage cream in cosmetics containing chestnut bark extract are shown in Table 10 below. Here, the chestnut skin extract refers to that of Example 20.
【0049】[0049]
【表10】 [Table 10]
【0050】[0050]
【処方例5】栗皮抽出物を含有した化粧料の中、パック
の処方例は次の表11である。ここで、栗皮抽出物は実
施例20のものをいう。[Formulation Example 5] Among the cosmetics containing the chestnut extract, a formulation example of the pack is shown in Table 11 below. Here, the chestnut skin extract refers to that of Example 20.
【0051】[0051]
【表11】 [Table 11]
【0052】[0052]
【発明の効果】一般に、天然物を含有する化粧料は肌に
対して補湿作用や美白作用の内、唯一つの効能を有する
が、本発明の栗皮抽出物を含有する化粧料は、皮膚美容
に効果的な遊離基の消去作用および補湿効果が優れ、さ
らにメラニン生成に関与する酵素であるチロシナーゼ活
性を抑制する効果による美白作用およびドーパ(DOPA)自
動酸化に対する阻害作用も優れてひとつの化粧料にて複
合的な効能を持つ化粧料である。In general, cosmetics containing natural products have only one of the moisturizing and whitening effects on the skin, but the cosmetics containing the chestnut bark extract of the present invention do not It excels in elimination and moisturizing effects of free radicals, which are effective for beauty.In addition, it has excellent whitening effect by inhibiting the activity of tyrosinase, an enzyme involved in melanin production, and excellent inhibitory effect on dopa (DOPA) autoxidation. It is a cosmetic that has multiple effects in cosmetics.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 FI A61K 7/00 A61K 7/00 M N (72)発明者 ベオム−ジュン キム 大韓民國,ソウル,カングセオ−グ,ワゴ ク 5−ドング,ウォオシン エーピーテ ィー.,9−403 (72)発明者 ヒュン−ピョ キム 大韓民國,ソウル,ソングパ−グ,バンギ −ドング 89,オリンピック セオンスチ ョン エーピーティー.,207−2301 (72)発明者 モオン−ヨウング ヘオ 大韓民國,カングウォンド,チュンチェオ ン−シ,トエギェ−ドング,グリーンタウ ン エーピーティー.,102−405──────────────────────────────────────────────────続 き Continuation of front page (51) Int.Cl. 6 Identification code FI A61K 7/00 A61K 7/00 M N (72) Inventor Beom-Jun Kim Republic of Korea, Seoul, Kangseog, Wagok 5-Dong , Wooshin API. , 9-403 (72) Inventor Hyun-Pyo Kim, Republic of Korea, Seoul, Songpaag, Bangui-Dong 89, Olympic Theoncheon Apty. , 207-2301 (72) Inventor Moong-Jongh-Heo Republic of Korea, Kangwon-do, Chuncheon-si, Toegye-Dong, Greentown AP. , 102-405
Claims (7)
化粧料組成物。1. A cosmetic composition comprising a chestnut bark extract.
皮であることを特徴とする請求項1記載の化粧料組成
物。2. The cosmetic composition according to claim 1, wherein the chestnut skin is chestnut carpel, which is a fruit of a chestnut tree plant.
ン、エチルアセテート、ジエチルエーテル、ベンゼン、
クロロホルム、ヘキサン、炭素数1〜4個の低級アルコ
ール、あるいは各々と水との混合物を溶媒として、冷却
コンデンサーを備えた抽出器で50〜95℃で、1〜2
0時間にわたって加熱する抽出方法により得ることを特
徴とする請求項1記載の化粧料組成物。3. The chestnut bark extract is prepared by converting chestnut bark into water or acetone, ethyl acetate, diethyl ether, benzene,
Using chloroform, hexane, a lower alcohol having 1 to 4 carbon atoms, or a mixture of each with water as a solvent, an extractor equipped with a cooling condenser at 50 to 95 ° C. for 1 to 2
The cosmetic composition according to claim 1, wherein the cosmetic composition is obtained by an extraction method in which heating is performed for 0 hours.
ン、エチルアセテート、ジエチルエーテル、ベンゼン、
クロロホルム、ヘキサン、炭素数1〜4個の低級アルコ
ール、あるいは各々と水との混合物を溶媒として、5〜
40℃で、1〜15日間にわたって沈積させて抽出する
方法により得ることを特徴とする請求項1記載の化粧料
組成物。4. The chestnut bark extract is prepared by converting chestnut bark into water or acetone, ethyl acetate, diethyl ether, benzene,
Chloroform, hexane, a lower alcohol having 1 to 4 carbon atoms, or a mixture of each with water as a solvent,
The cosmetic composition according to claim 1, wherein the cosmetic composition is obtained by a method of extracting by depositing at 40 ° C for 1 to 15 days.
て0.001〜10重量%の量で配合されていることを特
徴とする請求項1記載の化粧料組成物。5. The cosmetic composition according to claim 1, wherein the chestnut bark extract is incorporated in an amount of 0.001 to 10% by weight based on the dry weight of the cosmetic.
て0.01〜5重量%の量で配合されていることを特徴と
する請求項1記載の化粧料組成物。6. The cosmetic composition according to claim 1, wherein the chestnut skin extract is contained in an amount of 0.01 to 5% by weight based on the dry weight of the cosmetic.
ム、マッサージクリーム、エセンス、パックの剤形を有
することを特徴とする請求項1記載の化粧料組成物。7. The cosmetic composition according to claim 1, which has a dosage form of a softening lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, and a pack.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR19970004459 | 1997-02-14 | ||
KR97-4459 | 1997-02-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH10226633A true JPH10226633A (en) | 1998-08-25 |
Family
ID=19497034
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10029417A Pending JPH10226633A (en) | 1997-02-14 | 1998-02-12 | Cosmetic composition containing extract of chestnut bur |
Country Status (2)
Country | Link |
---|---|
JP (1) | JPH10226633A (en) |
KR (1) | KR100253843B1 (en) |
Cited By (5)
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JP2009506045A (en) * | 2005-08-26 | 2009-02-12 | イーエルシー マネージメント エルエルシー | Cosmetic composition containing a protease activator |
AU2005283330B2 (en) * | 2004-09-13 | 2011-11-24 | Chuon Co., Ltd. | Carbohydrase inhibitors derived from chestnut and use thereof |
CN102458156A (en) * | 2009-06-18 | 2012-05-16 | 株式会社爱茉莉太平洋 | Health food or pharmaceutical composition comprising chestnut shell extract |
JP2012530700A (en) * | 2009-06-18 | 2012-12-06 | 株式会社アモーレパシフィック | Cosmetic composition containing chestnut skin extract |
WO2021234270A1 (en) * | 2020-05-19 | 2021-11-25 | Fiabila | Cosmetic nail care composition in the form of a varnish |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20000065305A (en) * | 1999-04-01 | 2000-11-15 | 유상옥 | Skin care composition containing stabilized retinol, phytosphingosine, and Castaneae crenata extracts |
KR20010092070A (en) * | 2000-03-20 | 2001-10-24 | 유상옥,송운한 | Skin elasticity-improving cosmetic composition comprising Chestnut inner bark extract and Malt extract |
KR100428923B1 (en) * | 2001-06-08 | 2004-04-28 | 정한기 | Cream Composition |
KR100930122B1 (en) * | 2004-11-17 | 2009-12-04 | 하수용 | Herbal cosmetic composition containing natural plant extract derived from chestnut flower and method for producing same |
KR102085967B1 (en) * | 2018-03-28 | 2020-03-06 | 주식회사 메가코스바이오 | composition having anti-aging activity by using tomato and chestnut shell extract |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07126618A (en) * | 1993-11-04 | 1995-05-16 | Jumoku Chushutsu Seibun Riyou Gijutsu Kenkyu Kumiai | Antioxidant |
-
1997
- 1997-09-12 KR KR1019970047261A patent/KR100253843B1/en not_active IP Right Cessation
-
1998
- 1998-02-12 JP JP10029417A patent/JPH10226633A/en active Pending
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2005283330B2 (en) * | 2004-09-13 | 2011-11-24 | Chuon Co., Ltd. | Carbohydrase inhibitors derived from chestnut and use thereof |
JP2009506045A (en) * | 2005-08-26 | 2009-02-12 | イーエルシー マネージメント エルエルシー | Cosmetic composition containing a protease activator |
JP4845966B2 (en) * | 2005-08-26 | 2011-12-28 | イーエルシー マネージメント エルエルシー | Cosmetic composition containing a protease activator |
EP1916993A4 (en) * | 2005-08-26 | 2014-12-10 | Elc Man Llc | Cosmetic composition containing a protease activator |
CN102458156A (en) * | 2009-06-18 | 2012-05-16 | 株式会社爱茉莉太平洋 | Health food or pharmaceutical composition comprising chestnut shell extract |
JP2012530700A (en) * | 2009-06-18 | 2012-12-06 | 株式会社アモーレパシフィック | Cosmetic composition containing chestnut skin extract |
JP2012530699A (en) * | 2009-06-18 | 2012-12-06 | 株式会社アモーレパシフィック | Health food or pharmaceutical composition containing chestnut skin extract |
CN105285986A (en) * | 2009-06-18 | 2016-02-03 | 株式会社爱茉莉太平洋 | Health food or pharmaceutical composition comprising chestnut shell extract |
WO2021234270A1 (en) * | 2020-05-19 | 2021-11-25 | Fiabila | Cosmetic nail care composition in the form of a varnish |
FR3110412A1 (en) * | 2020-05-19 | 2021-11-26 | Fiabila | Cosmetic nail care composition in the form of a varnish |
Also Published As
Publication number | Publication date |
---|---|
KR19980069944A (en) | 1998-10-26 |
KR100253843B1 (en) | 2000-07-01 |
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