KR19980069944A - Cosmetic composition containing yulpi extract - Google Patents

Abstract

In the present invention, a mixture of water, acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, and a lower alcohol having 1 to 4 carbon atoms is used as a solvent to the inner skin of chestnut which is a fruit of wild or regenerated chestnut. The obtained extract is added to cosmetics, that is, softening cream (skin), nutritious cream (lotion), nourishing cream, massage cream, essence, pack and the like.

Description

Cosmetic composition containing yulpi extract

The present invention relates to a cosmetic composition containing an extract having a skin-improving effect, which is separated and purified from chestnut bark (Julpi), which is the fruit of the chestnut plant.

In general, cosmetics make skin clean, beautiful, and prevent aging. The main action is to moisturize the skin or to prevent excessive evaporation of moisture from the skin. Free radical scavenging and dopa (DOPA) automatics that inhibit or eliminate biological components (proteins, nucleic acids, cell membrane lipids, etc.) by oxidizing or denatured by highly reactive free radicals, free radicals and peroxides Inhibitory effect on oxidation, and the like.

Recently, various plant extracts have been researched, developed, and used as cosmetics, but they have only one effect such as the aforementioned moisturizing or whitening action.

In view of this reality, the present inventors have searched for an effective substance as a cosmetic for natural plants in order to find a better cosmetic. As a result, Yulpi extract has excellent safety and effective properties for various effects such as skin improvement and whitening effect. It became.

Chestnut trees include chestnut (Castanea crenata S. et Z., Castanea mollissima Bl., Castanea bulgaris), chestnut (Castanea Bungeana Bl), and chestnut (Castanea crenata for. Multicarpa (Uyeki) Chung). It is vertically raised to 100 ~ 1,100m and horizontally cures throughout Korea and is distributed in Japan and Manchuria. Leaves are alternate, elliptical or oblong lanceolate, blunt or heart-shaped, sharp at the ends, serrated. Flowers are hermaphrodite and bloom in May-June. Fruits are nuts, egg-shaped or obovate, parietal is spherical, thorny on the outside, ripens in September-October.

Yulpi is a dark brown bark that encloses the chestnut fruit and is separated from the chestnut fruit and dried in the sun.

It is an object of the present invention to provide a skin improvement cosmetic composition containing yulpi extract.

The present invention is described in more detail as follows.

The present invention relates to a cosmetic composition containing yulpi extract.

Yulpi extract of the present invention is contained in an amount of 0.001 to 10% by weight, preferably 0.01 to 5% by weight relative to the dry weight of the cosmetic.

The present invention is a mixture of organic solvents, such as water or acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, lower alcohols having 1 to 4 carbon atoms, or water in chestnuts of wild or cultivated chestnut. The extract obtained as a solvent is added to cosmetics, that is, softening cream (skin), nutrient cream (lotion), nourishing cream, massage cream, essence, pack and the like.

To explain in more detail the recipe of the extract of the present invention, the skin of chestnut (Julpi) was washed with clean water, dried and finely powdered, and powdered Yulpi powder as an extraction solvent as water, acetone, ethyl acetate, di Ethyl ether, benzene, chloroform, and lower alcohols having 1 to 4 carbon atoms, respectively or mixed with water, are added 1 to 15 times to the dry weight of Yulpi powder, and 50 ~ with the cooling capacitor installed to prevent the active ingredient from evaporating. Extract by heating at 95 ℃ for 1 to 20 hours, or by dipping at 5 ~ 40 ℃ for 1 to 15 days to extract the active ingredient. The extract thus obtained is concentrated under reduced pressure using a distillation apparatus equipped with a cooling capacitor to prepare the extract of the present invention.

Cosmetics containing the yulpi extract of the present invention has a formulation such as supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence and pack.

The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Example 1

After washing with purified water and drying, dried powdered Yulpi powder 1Kg in 5L of water, extracted by heating for 5 hours in an extractor with a cooling capacitor, filtered with 300 mesh filter cloth and left at 4 ~ 15 ℃ for 7 days, and then aged at low temperature. , Filter with Whatman No. 2 filter paper. The filtrate was dried at 60 deg. C using a rotary evaporator to obtain 17.5 g (dry weight).

Example 2

After washing with purified water and drying, 1Kg of powdered Yulpi powder was added to 5L of 10% ethanol and extracted by heating for 3 hours in an extractor equipped with a cooling condenser, filtered through 300 mesh filter cloth, and left at 4-15 ° C. for 7 days. After filtration, the filter was filtered using Whatman No. 2 filter paper. This filtrate was dried on a rotary evaporator at 60 ° C. to obtain 21.8 g (dry weight).

Examples 3-11

Extraction was carried out in the same manner as in Example 2, and the results are shown in Table 1 below.

Experimental Results of Examples 3-11 Extraction solvent Loss on Drying of Final Extract (Unit g) Example 3 20% ethanol 22.3 Example 4 30% ethanol 22.5 Example 5 40% ethanol 25.8 Example 6 50% ethanol 26.3 Example 7 60% ethanol 25.9 Example 8 70% ethanol 29.3 Example 9 80% ethanol 31.3 Example 10 90% ethanol 33.1 Example 11 100% ethanol 36.5

Example 12

After washing with purified water and drying, 1Kg of powdered Yulpi powder was added to 5L of water, extracted for 3 days at 15 ~ 40 ℃, filtered through 300 mesh filter cloth, left at 4 ~ 15 ℃ for 7 days, and then aged at low temperature. Filter with filter paper 2. The filtrate was dried at 60 ° C. using a rotary evaporator to obtain 40.5 g (dry weight).

Example 13

After washing with purified water and drying, 1Kg of powdered Yulpi powder was added to 5L of 10% ethanol, extracted at 15-40 ° C. for 3 days, filtered through 300 mesh filter cloth, left at 4-15 ° C. for 7 days, and then aged at low temperature. Filter with Whatman No. 2 filter paper. This filtrate was dried on a rotary evaporator at 60 ° C. to obtain 38.9 g (dry weight).

Examples 14-34

The extraction in the same manner as in Example 13 and the results are shown in Table 2 below.

Experimental Results of Examples 14-34 Extraction solvent Loss on Drying of Final Extract (Unit: g) Example 14 20% ethanol 47.7 Example 15 30% ethanol 50.2 Example 16 40% ethanol 60.1 Example 17 50% ethanol 68.4 Example 18 60% ethanol 78.7 Example 19 70% ethanol 83.5 Example 20 80% ethanol 85.9 Example 21 90% ethanol 87.3 Example 22 100% ethanol 86.2 Example 23 80% methanol 86.9 Example 24 n-propanol 60.8 Example 25 Isopropanol 65.1 Example 26 2-butanol 32.7 Example 27 Acetone 27.5 Example 28 Ethyl acetate 19.8 Example 29 Ethyl ether 21.5 Example 30 benzene 23.6 Example 31 chloroform 55.3 Example 32 n-hexane 64.2 Example 33 Butyl acetate 24.4 Example 34 1,3-butylene glycol 30.6

Experimental Example 1

Tyrosinase inhibitory effect was measured on the yulpi extract.

1) experimental method

The inhibitory effect of tyrosinase activity was measured on the yulpi extracts prepared in Examples 1 to 34. Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. Tyrosine, a substrate, was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) and used as a 0.1 mg / ml solution. 0.5 ml of various extracts of the present invention are mixed at a low titer and left for 10 minutes at 37 ° C. incubator, and 0.5 ml of 300 U / ml tyrosinase is added thereto and reacted at 37 ° C. for 10 minutes. At this time, the control is 0.5ml of buffer solution. After the reaction, the test tube was placed on ice and quenched to stop the reaction, and the absorbance at 475 nm was measured with a spectrophotometer.

Tyrosinase inhibitory effect of each yulpi extract is calculated by the following formula.

Reaction absorbance of each extract

Tyrosinase Inhibition Rate (%) = 100-(------------------------- X 100)

Response absorbance of the control

2) Experiment result

As a result of measuring the absorbance to measure the tyrosinase inhibitory effect, the IC ₅₀ concentration of the lyophyll extract obtained by the method of Example 20 was 80 μg / ml.

Experimental results of Examples 1 to 34 are as shown in Table 3 below.

Inhibitory Effect of Yulpi Extract on Tyrosinase Activity Experimental substance Final test concentration (%, W / V) Tyrosinase activity inhibition rate (%) Example 1 0.0150 74.1 Example 2 0.0150 67.9 Example 3 0.0150 71.9 Example 4 0.0150 77.3 Example 5 0.0150 69.2 Example 6 0.0150 80.5 Example 7 0.0150 77.7 Example 8 0.0150 70.7 Example 9 0.0150 73.8 Example 10 0.0150 85.8 Example 11 0.0150 86.4 Example 12 0.0150 88.2 Example 13 0.0150 83.3 Example 14 0.0150 82.3 Example 15 0.0150 86.9 Example 16 0.0150 80.1 Example 17 0.0150 87.2 Example 18 0.0150 90.7 Example 19 0.0150 92.6 Example 20 0.0150 94.6 Example 21 0.0150 88.9 Example 22 0.0150 90.2 Example 23 0.0150 86.8 Example 24 0.0150 93.5 Example 25 0.0150 50.6 Example 26 0.0150 33.0 Example 27 0.0150 29.1 Example 28 0.0150 47.8 Example 29 0.0150 55.9 Example 30 0.0150 39.5 Example 31 0.0150 42.7 Example 32 0.0150 21.1 Example 33 0.0150 46.6 Example 34 0.0150 48.8

Experimental Example 2

Inhibition of dopa (DOPA) automatic oxidation was measured for the yulpi extract.

1) Experiment Method

Inhibition was measured by the following method.

Buffer sodium-phosphate (0.05M, pH 6.8) and DOPA (500μM) were prepared and the total reaction volume was 2ml and reacted for 48 hours at 37 ℃ to measure the absorbance at 475nm. The yul extract was dissolved in a buffer solution and added.

The inhibition effect of DOPA automatic oxidation of each yul extract is calculated by the following formula.

Reaction absorbance of each extract

DOPA autooxidation inhibition rate (%) = 100-(------------------------- X 100)

Response absorbance of the control

2) Experiment result

The absorbance was measured to measure the effect of inhibiting the DOPA autooxidation. As a result, the IC ₅₀ concentration for the DOPA autooxidation of Example 20 extract was 746.67 µg / ml.

Yield extracts of Examples 1 to 34 are as shown in Table 4 below.

Inhibitory Effect of Yul-Pi Extract on DOPA Experimental substance Final experiment concentration (%, W / V) DOPA automatic oxidation inhibition rate (%) Example 1 0.150 70.5 Example 2 0.150 77.7 Example 3 0.150 50.8 Example 4 0.150 67.1 Example 5 0.150 55.6 Example 6 0.150 54.1 Example 7 0.150 31.2 Example 8 0.150 45.8 Example 9 0.150 63.3 Example 10 0.150 45.9 Example 11 0.150 80.1 Example 12 0.150 59.4 Example 13 0.150 64.2 Example 14 0.150 67.9 Example 15 0.150 62.2 Example 16 0.150 71.2 Example 17 0.150 75.5 Example 18 0.150 82.6 Example 19 0.150 98.8 Example 20 0.150 90.5 Example 21 0.150 92.1 Example 22 0.150 92.4 Example 23 0.150 80.7 Example 24 0.150 89.9 Example 25 0.150 74.9 Example 26 0.150 63.1 Example 27 0.150 56.8 Example 28 0.150 43.4 Example 29 0.150 65.5 Example 30 0.150 76.3 Example 31 0.150 66.6 Example 32 0.150 37.5 Example 33 0.150 61.7 Example 34 0.150 78.4

Experimental Example 3

The free radical scavenging effect on the extract was measured.

1) Experiment Method

Free radical scavenging activity was assayed by the following method.

2ml of 60μM DPPH (1,1-diphenyl-2-picrylhydrazyl) solution was added with 1ml of Yulpi extract at the proper concentration, and then reacted at 37 ° C for 30 minutes, and the absorbance was measured at 520nm.

At this time, each yulpi extract was dissolved in DMSO (dimethyl sulfoxide).

2) Experiment result

Measurement of absorbance with IC ₅₀ of free-radical scavenging activity of yulpi extract obtained by the method in Example 20 results of measuring a free-radical scavenging activity was 8㎍ / ml. This value is a powerful free radical scavenging agent gallic acid (Gallic acid) IC ₅₀ in 1.5㎍ / and might be compared to the active ml, powerful antioxidants known chemical synthesis _{BHT (IC 50: 37㎍ / ml} ) of or ascorbic biksan ( IC _50: showed higher activity than 29㎍ / ml).

Experimental results of the yeast extract obtained by the method of Examples 1 to 34 are as shown in Table 5 below.

Free Radical Scavenging Activity of Yulpi Extract Experimental substance Final test concentration (%, W / V) Free radical scavenging ability (%) Example 1 0.00150 66.7 Example 2 0.00150 63.1 Example 3 0.00150 75.4 Example 4 0.00150 80.2 Example 5 0.00150 58.9 Example 6 0.00150 43.1 Example 7 0.00150 55.8 Example 8 0.00150 48.9 Example 9 0.00150 64.6 Example 10 0.00150 77.4 Example 11 0.00150 71.2 Example 12 0.00150 69.9 Example 13 0.00150 74.5 Example 14 0.00150 79.5 Example 15 0.00150 83.3 Example 16 0.00150 84.7 Example 17 0.00150 88.5 Example 18 0.00150 90.9 Example 19 0.00150 95.9 Example 20 0.00150 98.3 Example 21 0.00150 97.3 Example 22 0.00150 98.2 Example 23 0.00150 96.5 Example 24 0.00150 86.3 Example 25 0.00150 60.1 Example 26 0.00150 45.9 Example 27 0.00150 47.8 Example 28 0.00150 55.5 Example 29 0.00150 75.4 Example 30 0.00150 65.8 Example 31 0.00150 51.2 Example 32 0.00150 70.4 Example 33 0.00150 63.2 Example 34 0.00150 38.8

Experimental Example 4

Moisturizing effect on the yulpi extract was measured.

1) Experiment Method

Tiwamita TM210 (C +), a transdermal moisture loss measuring instrument, was applied to the upper arm or hand of the arm three times a day for each of the nourishing cream composition and the non-containing composition prepared according to the method of Formulation 3 K Electronic GmbH, Germany) was used to measure transepidermal water loss (TEWL) by time zone, and the moisturizing effect was measured by the change of TEWL value (△ TEWL) with time. At this time, the experimental conditions were 25 ℃, relative humidity was carried out in a constant temperature and humidity room of 45%. Moisturizing effect by quantifying the moisture content of the skin from 0 to 150 using numerical value of the change in electrical conductivity with respect to the change of epidermal moisture level using Koniometa CM 820 PC (C + K electronic GmbH, Germany) Was measured.

2) Experiment result

The nutrition cream composition containing Yul extract was 1.6 ± 0.4 (g / hm ² ) lower in ΔTEWL than the composition without Yul extract, and the epidermal moisture content was lower than the composition containing no Yul extract. On average, it shows a high value of 10 or more, indicating that the extract has moisturizing effect.

Comparative Example 1

The resultant was washed with purified water and extracted in the same manner as in Example 23 with 1 g of dry gallnut to obtain a dried product.

Comparative Example 2

After washing with purified water and drying dried 1Kg extracted in the same manner as in Example 23 to obtain a dried product.

Comparative Example 3

After washing with purified water and extracting the dried fat milk in the same manner as in Example 23 to obtain a dried product.

Comparative Example 4

Washed with purified water and extracted with 1Kg of dry cornus oil in the same manner as in Example 23 to obtain a dried product.

Experimental Example 5

Inhibition of tyrosinase activity, inhibition of dopa autooxidation, and free radical scavenging according to the concentrations of Yulpi extract and the extracts prepared in Comparative Examples 1 to 4 were measured by the same method as Experimental Examples 1 to 3.

The biological activities of the extract of Yulpi were compared with the extracts of Gallja, Gaza, fat milk and cornus oil containing high amounts of tannin. The experimental results are shown in Table 6 below.

Comparison of Bioactivity of Plant Extract Containing Yulpi and Tannin Tyrosinase activity inhibition (%) DOPA Automatic Oxidation Inhibition Free radical scavenging 33 (μg / l) 333 (μg / l) 100 (μg / l) 500 (μg / l) 10 (μg / l) 1000 (μg / l) Example 24 31 73 3 10 41 12 Comparative Example 1 80 90 0 0 58 17 Comparative Example 2 0 38 0 0 64 69 Comparative Example 3 0 19 0 0 46 33 Comparative Example 4 0 0 0 0 4 69

Yulpi extract exhibits high activity in both tyrosinase activity inhibition, dopa autooxidation inhibition and free radical scavenging activity, compared to other tannin-containing plant extracts.

Prescription Example 1

A prescription example of skin softener (skin) in cosmetics containing yulpi extract is as follows.

Here, yulpi extract refers to that of Example 20.

ingredient Content (unit: weight%) Yulpi Extract 0.1 1,3-butylene glycol 5.0 glycerin 5.0 ethanol 10.0 Oleyl alcohol 0.1 Poly Sorbate 20 0.5 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example 2

A prescription example of nourishing lotion (lotion) in cosmetics containing yulpi extract is as follows.

Here, yulpi extract refers to that of Example 20.

ingredient Content (unit: weight%) Yulpi Extract 1.0 1,3-butylene glycol 5.0 glycerin 5.0 Triethanolamine 1.2 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Solbitan Sesqui Olate 1.0 Carboxy Vinyl Polymer 1.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example 3

A prescription example of nutrition cream in cosmetics containing yulpi extract is as follows.

Here, yulpi extract refers to that of Example 20.

ingredient Content (unit: weight%) Yulpi Extract 2.0 Propylene glycol 5.0 glycerin 5.0 Triethanolamine 0.5 Liquid paraffin 10.0 Squalane 3.0 vaseline 7.0 Beeswax 2.0 Polysorbate 60 2.0 Solbitan Sesqui Olate 2.5 Carboxy Vinyl Polymer 0.5 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example 4

A prescription example of a massage cream in cosmetics containing yulpi extract is as follows.

Here, yulpi extract refers to that of Example 20.

ingredient Content (unit: weight%) Yulpi Extract 0.3 1,3-butylene glycol 5.0 glycerin 5.0 ethanol 7.0 Triethanolamine 0.5 Liquid paraffin 30.0 Beeswax 2.0 Stearyl alcohol 2.0 Polysorbate 60 3.0 Solbitan Sesqui Olate 2.5 Carboxy Vinyl Polymer 0.5 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example 5

A prescription example of a pack in cosmetics containing yulpi extract is as follows.

Here, yulpi extract refers to that of Example 20.

ingredient Content (unit: weight%) Yulpi Extract 3.0 Propylene glycol 2.0 glycerin 4.0 Triethanolamine 0.3 Carboxy Vinyl Polymer 0.3 Fiji-40-Hydrogenated Castor Oil 0.8 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Generally, cosmetics containing natural products have one effect of moisturizing or whitening effect on skin, but cosmetics containing Yulpi extract of the present invention have excellent free radical scavenging and moisturizing effects, which are effective for skin beauty, and melanin. Inhibition of the whitening and dopa (DOPA) automatic oxidation due to the inhibitory effect of tyrosinase activity, an enzyme involved in the production is also excellent because it can have a complex effect as one cosmetic.

Claims (7)

Cosmetic composition containing yulpi extract. The cosmetic composition according to claim 1, wherein the skin is a bark of the chestnut, which is the fruit of the chestnut plant. According to claim 1, Yulpi extract is a cold condensate attached to the yulpi extracted by using water, acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, a lower alcohol having 1 to 4 carbon atoms as a solvent or mixed with water. Cosmetic composition characterized in that obtained by the extraction method of heating for 1 to 20 hours at 50 ~ 95 ℃ in the extractor. According to claim 1, Yulpi extract is 5 ~ 40 ℃ by using a solvent which is a mixture of water, acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, lower alcohol having 1 to 4 carbon atoms, or water, respectively. Cosmetic composition, characterized in that obtained by the method of immersion and extraction for 1 to 15 days. The cosmetic composition according to claim 1, wherein the yulpi extract is contained in an amount of 0.001 to 10% by weight based on the dry weight of the cosmetic. The cosmetic composition according to claim 1, wherein the yulpi extract is contained in an amount of 0.01 to 5% by weight based on the dry weight of the cosmetic. The cosmetic composition according to claim 1, which has a formulation of softening cream, nutrient cream, nourishing cream, massage cream, essence and pack.