JPH09509052A - 肝炎c型ウイルスエンベロープ遺伝子用の哺乳動物発現系 - Google Patents
肝炎c型ウイルスエンベロープ遺伝子用の哺乳動物発現系Info
- Publication number
- JPH09509052A JPH09509052A JP7520185A JP52018595A JPH09509052A JP H09509052 A JPH09509052 A JP H09509052A JP 7520185 A JP7520185 A JP 7520185A JP 52018595 A JP52018595 A JP 52018595A JP H09509052 A JPH09509052 A JP H09509052A
- Authority
- JP
- Japan
- Prior art keywords
- hcv
- antibody
- mammalian expression
- fusion protein
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
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- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
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- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/82—Hepatitis associated antigens and antibodies
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.プラスミドpHCV−176。 2.プラスミドpHCV−172。 3.プラスミドpHCV−351。 4.プラスミドpHCV−425。 5.哺乳動物発現ベクターpHCV−172により発現されたAPP−HCV− E1融合タンパク質。 6.哺乳動物発現ベクターpHCV−351により発現されたAPP−HCV− E2融合タンパク質。 7.哺乳動物発現ベクターpHCV−425により発現されたAPP−HCV− E1−E2融合タンパク質。 8.HCV抗原又は抗体を含むおそれのあるテスト試料中のHCV抗原又は抗体 を検出する方法であって、その改良が、テスト試料と、pHCV172、pHC V351又はpHCV425からなる群から選択されたグリコシル化HCVエン ベロープ抗原融合タンパク質とを接触させることからなり、前記融合タンパク質 が哺乳動物発現系中で産生される前記方法。 9.HCV抗原又は抗体を含むおそれのあるテスト試料中 のHCV抗原又は抗体を検出する方法であって、その改良が、テスト試料と、p HCV172、pHCV351又はpHCV425からなる群から選択されたグ リコシル化HCVエンベロープ抗原融合タンパク質を用いて産生された抗体とを 接触させることからなり、前記融合タンパク質が哺乳動物発現系中で産生される 前記方法。 10.HCV抗原又は抗体を含むおそれのあるテスト試料中の該HCV抗原又は HCV抗体の存在を検出するためのテストキットであって、 哺乳動物発現系中で産生されたpHCV172、pHCV351又はpHCV 425からなる群から選択されたグリコシル化HCVエンベロープ抗原融合タン パク質を含む容器を含む前記テストキット。 11.哺乳動物発現系中で産生されたpHCV172、pHCV351又はpH CV425からなる群から選択されたグリコシル化HCVエンベロープ抗原融合 タンパク質を用いて産生された抗体を含む容器をさらに含む請求項10に記載の テストキット。 12.HCV抗原又はHCV抗体を含むおそれのあるテスト試料中の該HCV抗 原又はHCV抗体の存在を検出する ためのテストキットであって、 哺乳動物発現系中で産生されたpHCV172、pHCV351又はpHCV 425からなる群から選択されたグリコシル化HCVエンベロープ抗原融合タン パク質を用いて産生された抗体を含む容器をさらに含む前記テストキット。 13.医薬上許容し得る賦形剤中に、哺乳動物細胞中のpHCV172、pHC V351又はpHCV425からなる群から選択された融合タンパク質から発現 された免疫原性グリコシル化HCVタンパク質を薬理学上有効量含むHCV感染 症治療用ワクチン。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/188,281 | 1994-01-28 | ||
US08/188,281 US5610009A (en) | 1994-01-28 | 1994-01-28 | Mammalian expression systems for hepatitis C virus envelope genes |
PCT/US1995/001087 WO1995020664A2 (en) | 1994-01-28 | 1995-01-27 | Mammalian expression systems for hepatitis c virus envelope genes |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH09509052A true JPH09509052A (ja) | 1997-09-16 |
Family
ID=22692507
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP7520185A Pending JPH09509052A (ja) | 1994-01-28 | 1995-01-27 | 肝炎c型ウイルスエンベロープ遺伝子用の哺乳動物発現系 |
Country Status (9)
Country | Link |
---|---|
US (1) | US5610009A (ja) |
EP (1) | EP0741787B1 (ja) |
JP (1) | JPH09509052A (ja) |
AT (1) | ATE244762T1 (ja) |
AU (1) | AU1692595A (ja) |
CA (1) | CA2182170A1 (ja) |
DE (1) | DE69531235T2 (ja) |
ES (1) | ES2207645T3 (ja) |
WO (1) | WO1995020664A2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2006507328A (ja) * | 2002-11-08 | 2006-03-02 | ザ アドミニストレイターズ オブ ザ テューレイン エデュケイショナル ファンド | フラビウイルス融合インヒビター |
Families Citing this family (16)
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US20040185061A1 (en) * | 1994-07-29 | 2004-09-23 | Innogenetics N.V. | Redox reversible HCV proteins with native-like conformation |
EP1211315A1 (en) * | 1994-07-29 | 2002-06-05 | Innogenetics N.V. | Recombinant vectors for producing HCV envelope proteins |
JPH11507129A (ja) * | 1995-06-07 | 1999-06-22 | アボツト・ラボラトリーズ | C型肝炎ウイルス第二エンベロープ糖タンパク質に対する抗体の検出法 |
US20050074752A1 (en) * | 1996-06-11 | 2005-04-07 | Merck & Co., Inc. | Synthetic hepatitis C genes |
AU717542B2 (en) * | 1996-06-11 | 2000-03-30 | Merck & Co., Inc. | Synthetic hepatitis C genes |
EP0947525A1 (en) | 1998-03-27 | 1999-10-06 | Innogenetics N.V. | Epitopes in viral envelope proteins and specific antibodies directed against these epitopes: use for detection of HCV viral antigen in host tissue |
AU4335099A (en) * | 1998-06-18 | 2000-01-05 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Surface targeted expression of a modified hepatitis c virus envelope protein |
US7108855B2 (en) * | 1998-06-24 | 2006-09-19 | Innogenetics N.V. | Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use |
US6562346B1 (en) * | 1999-10-27 | 2003-05-13 | Chiron Corporation | Activation of HCV-specific T cells |
US7101561B2 (en) | 2000-12-01 | 2006-09-05 | Innogenetics N.V. | Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use |
US20030215896A1 (en) * | 2001-04-25 | 2003-11-20 | Yueming Li | Gamma secretase substrates and in vitro assays |
US20030152942A1 (en) * | 2001-05-09 | 2003-08-14 | Lance Fors | Nucleic acid detection in pooled samples |
WO2003008541A2 (en) * | 2001-07-16 | 2003-01-30 | Eli Lilly And Company | Extracellular junctional adhesion molecules |
AU2005295317B2 (en) * | 2004-10-18 | 2011-10-13 | Globeimmune, Inc. | Yeast-based therapeutic for chronic hepatitis C infection |
AU2015234338C1 (en) * | 2006-07-28 | 2017-07-20 | The Trustees Of The University Of Pennsylvania | Improved vaccines and methods for using the same |
WO2009034190A2 (en) * | 2007-09-14 | 2009-03-19 | Genimmune N.V. | Affinity tag |
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US5523215A (en) * | 1985-03-28 | 1996-06-04 | Chiron Corporation | Enhanced purification and expression of insoluble recombinant proteins |
ES2012739T5 (es) * | 1987-11-18 | 2001-12-01 | Chiron Corp | Diagnosticos para nanbv. |
YU48038B (sh) * | 1987-11-18 | 1996-10-18 | Chiron Corp. | Postupak za dijagnostiku ne-a, ne-v hepatitisa |
US5350671A (en) * | 1987-11-18 | 1994-09-27 | Chiron Corporation | HCV immunoassays employing C domain antigens |
HU225068B1 (en) * | 1989-03-17 | 2006-05-29 | Chiron Corp | Process for producing diagnostics and vaccine of nanbh |
US5106726A (en) * | 1990-02-16 | 1992-04-21 | United Biomedical, Inc. | Synthetic peptides specific for the detection of antibodies to HCV |
SK286106B6 (sk) * | 1990-11-08 | 2008-03-05 | Novartis Vaccines & Diagnostics, Inc. | Spôsob vykonávania imunodiagnostickej skúšky na detekciu protilátky proti vírusu hepatitídy C |
AU2513592A (en) * | 1991-08-21 | 1993-03-16 | Abbott Laboratories | Hepatitis c assay utilizing recombinant antigens to ns1 |
JP3439209B2 (ja) * | 1992-01-31 | 2003-08-25 | アボツト・ラボラトリーズ | Hcv蛋白のための哺乳動物発現システム |
JPH05344899A (ja) * | 1992-06-11 | 1993-12-27 | Kokuritsu Yobou Eisei Kenkyusho | C型肝炎ウイルス外被タンパク質の産生法 |
-
1994
- 1994-01-28 US US08/188,281 patent/US5610009A/en not_active Expired - Lifetime
-
1995
- 1995-01-27 EP EP95908700A patent/EP0741787B1/en not_active Expired - Lifetime
- 1995-01-27 WO PCT/US1995/001087 patent/WO1995020664A2/en active Search and Examination
- 1995-01-27 DE DE69531235T patent/DE69531235T2/de not_active Expired - Lifetime
- 1995-01-27 JP JP7520185A patent/JPH09509052A/ja active Pending
- 1995-01-27 AU AU16925/95A patent/AU1692595A/en not_active Abandoned
- 1995-01-27 AT AT95908700T patent/ATE244762T1/de not_active IP Right Cessation
- 1995-01-27 ES ES95908700T patent/ES2207645T3/es not_active Expired - Lifetime
- 1995-01-27 CA CA002182170A patent/CA2182170A1/en not_active Abandoned
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006507328A (ja) * | 2002-11-08 | 2006-03-02 | ザ アドミニストレイターズ オブ ザ テューレイン エデュケイショナル ファンド | フラビウイルス融合インヒビター |
Also Published As
Publication number | Publication date |
---|---|
WO1995020664A3 (en) | 1995-12-28 |
ATE244762T1 (de) | 2003-07-15 |
US5610009A (en) | 1997-03-11 |
AU1692595A (en) | 1995-08-15 |
DE69531235T2 (de) | 2004-05-13 |
EP0741787A1 (en) | 1996-11-13 |
WO1995020664A2 (en) | 1995-08-03 |
DE69531235D1 (de) | 2003-08-14 |
EP0741787B1 (en) | 2003-07-09 |
CA2182170A1 (en) | 1995-08-03 |
ES2207645T3 (es) | 2004-06-01 |
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