JPH08507207A - グラム陽性菌中の商業的に重要な菌体外タンパク質の高められた生産のための方法及び系 - Google Patents
グラム陽性菌中の商業的に重要な菌体外タンパク質の高められた生産のための方法及び系Info
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- JPH08507207A JPH08507207A JP6518691A JP51869194A JPH08507207A JP H08507207 A JPH08507207 A JP H08507207A JP 6518691 A JP6518691 A JP 6518691A JP 51869194 A JP51869194 A JP 51869194A JP H08507207 A JPH08507207 A JP H08507207A
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.野生型よりも多くのPrsAタンパク質又はその機能的類似体を発現するこ とができ、かつ野生型よりも多くの少なくとも1つの目的の細胞外タンパク質を 発現することができるグラム陽性細菌を含む、グラム陽性細菌における細胞外タ ンパク質の分泌を促進するための発現系。 2.前記PrsAタンパク質は前記グラム陽性細菌と同種のものである請求項1 記載の発現系。 3.前記PrsAタンパク質は前記グラム陽性細菌と異種のものである請求項1 記載の発現系。 4.前記PrsAタンパク質は、バチルス属の種のPrsAタンパク質である請 求項1記載の発現系。 5.前記PrsAタンパク質は枯草菌、バチルス・アミロリクエファシエンス又 はバチルス・リケニホルミスのPrsAタンパク質である請求項4記載の発現系 。 6.前記PrsAタンパク質は、枯草菌、バチルス・アミロリクエファシエンス 又はバチルス・リケニホルミスのPrsAタンパク質に対する抗体と免疫学的に 反応し、かつ、過剰発現された場合には、前記グラム陽性細菌からの前記目的の 細胞外タンパク質の分泌を促進することができる請求項1記載の発現系。 7.前記PrsAタンパク質又はその機能的類似体は、前記グラム陽性細菌細胞 中に、野生型の2倍ないし約10倍量存在する請求項1記載の発現系。 8.前記目的の細胞外タンパク質はプロテアーゼ、リパーゼ、クチナーゼ、アミ ラーゼ、ガラクトシダーゼ、プルラナーゼ、セルラーゼ、グルコースイソメラー ゼ、タンパクジスフィドイソメラーゼ、CGT’ase(シクロデキストリング ルコノトランスフェラーゼ)、フィターゼ、グルコースオキシダーゼ、グルコシ ルトランスフェラーゼ、ラッカーゼ、ビリルビンオキシダーゼ、キシラナーゼ、 抗原性微生物又は原生動物タンパク質、細菌性タンパク質毒素、微生物表面タン パク質、ウイルスタンパク質又は医薬である請求項1記載の発現系。 9.前記目的の細胞外タンパク質は、該タンパク質をコードする構造遺伝子にシ グナル配列を付加することによって創製された非ネイティブ細胞外タンパク質で ある請求項8記載の発現系。 10.前記グラム陽性細菌はバチルス属の種である請求項1ないし9のいずれか 1項に記載の発現系。 11.前記バチルスは枯草菌、バチルス・リケニホルミス、バチルス・レンタス 、バチルス・ブレビス、バチルス・ステアロサーモフィルス、バチルス・アルカ リフィルス、バチルス・アミロリクエファシエンス、バチルス・コーギュランス 、バチルス・サーキュランス、バチルス・ロータス又はバチルス・スリンジエン シスである請求項10記載の発現系。 12.少なくとも1つの目的の細胞外タンパク質を野生型よりも多く発現するこ とができ、かつpKTH277を含むグラム陽性細菌。 13.少なくとも1つの目的の細胞外タンパク質を野生型よりも多く発現するこ とができ、かつ、少なくとも2コピーの枯草菌のPrsA遺伝子、又はprsA 遺伝子若しくはその機能的類似体の過剰発現をもたらす強力な調節配列に機能的 に連結された枯草菌のprsA遺伝子若しくはその機能的類似体を含むグラム陽 性細菌。 14.前記グラム陽性細菌はバチルス属に属する請求項12又は13項記載のグ ラム陽性細菌。 15.前記バチルスは枯草菌、バチルス・リケニホルミス、バチルス・レンタス 、バチルス・ブレビス、バチルス・ステアロサーモフィルス、バチルス・アルカ リフィルス、バチルス・アミロリクエファシエンス、バチルス・コーギュランス 、バチルス・サーキュランス、バチルス・ロータス又はバチルス・スリンジエン シスである請求項14記載のグラム陽性細菌。 16.枯草菌のprsA遺伝子又はその機能的類似体の過剰発現を引き起こす発 現シグナルの制御下に枯草菌のprsA遺伝子又はその機能的類似体を含むDN A構築物。 17.枯草菌のprsA遺伝子又はその機能的類似体の過剰発現を引き起こす発 現シグナルの制御下に枯草菌のprsA遺伝子又はその機能的類似体をさらに含 むベクター。 18.目的の細胞外タンパク質を野生型よりも多く発現することができるグラム 陽性細菌中で、枯草菌のPrsAタンパク質又はその機能的類似体を野生型より も多く発現させることを含む、グラム陽性細菌による目的の細胞外タンパク質の 分泌を促進する方法。 19.前記グラム陽性細菌はバチルス属に属する請求項18記載の方法。 20.前記バチルスは枯草菌、バチルス・リケニホルミス、バチルス・レンタス 、バチルス・ブレビス、バチルス・ステアロサーモフィルス、バチルス・アルカ リフィルス、バチルス・アミロリクエファシエンス、バチルス・コーギュランス 、バチルス・サーキュランス、バチルス・ロータス又はバチルス・スリンジエン シスである請求項19記載の方法。 21.(a)枯草菌のPrsAタンパク質の機能的類似体をコードする、非枯草 菌グラム陽性細菌宿主の遺伝子を同定し、(b)該遺伝子のコピーを少なくとも 1つさらに前記宿主生物に導入するか又は該遺伝子の過剰発現をもたらす発現配 列に機能的に連結された前記遺伝子を前記宿主生物に導入することにより、(a )工程で同定された前記遺伝子の発現を高めることを含む、宿主生物中で過剰発 現される目的の細胞外タンパク質の高められた分泌のために有用な非枯草菌グラ ム陽性細菌宿主生物の創製方法。 22.サザンブロット法により、枯草菌のprsA遺伝子からのDNAプローブ とハイブリダイズするDNAを同定し、該遺伝子が、過剰発現されると目的の細 胞外タンパク質の分泌についてグラム陽性細菌の分泌能力が高められるタンパク 質をコードすることを示すことを含む、枯草菌のPrsAの機能的類似体をコー ドする遺伝子を同定する方法。 23.高力価の抗PrsA抗体と反応するタンパク質を同定し、該タンパク質が グラム陽性細菌中に野生型よりも多く存在すると、目的の細胞外タンパク質の分 泌について該グラム陽性細菌の分泌能を高めることができることを示すことを含 む、枯草菌のPrsAの機能的類似体をコードする遺伝子を同定する方法。
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DE (1) | DE69434807T2 (ja) |
DK (1) | DK0686195T3 (ja) |
WO (1) | WO1994019471A1 (ja) |
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US7118757B1 (en) * | 1988-12-19 | 2006-10-10 | Wyeth Holdings Corporation | Meningococcal class 1 outer-membrane protein vaccine |
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US5945278A (en) * | 1996-07-08 | 1999-08-31 | The Finnish National Public Health Institute | Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria |
US5919999A (en) * | 1996-11-14 | 1999-07-06 | Queen's University At Kingston | Enhanced transport with a plastid membrane transport protein |
US6506579B1 (en) | 1997-07-15 | 2003-01-14 | Genencor International, Inc. | Increasing production of proteins in gram-positive microorganisms using SecG |
AU8405698A (en) * | 1997-07-15 | 1999-02-10 | Genencor International B.V. | Increasing production of proteins in gram-positive microorganisms |
US20030157642A1 (en) | 1997-07-15 | 2003-08-21 | Caldwell Robert M. | Increasing production of proteins in gram-positive microorganisms |
EP1003873B1 (en) * | 1997-07-16 | 2005-04-06 | Genencor International, Inc. | Increasing production of proteins in gram-positive microorganisms |
ES2259449T3 (es) * | 1997-12-05 | 2006-10-01 | Virginia Tech Intellectual Properties, Inc. | Vacuna de antigeno homologo sobreexpresado y procedimiento para la preparacion de la misma. |
EP1038959A1 (en) | 1999-03-17 | 2000-09-27 | Aventis Behring Gesellschaft mit beschränkter Haftung | Factor VIII without B-domain, comprising one or more insertions of a truncated intron I of factor IX |
WO2004101889A2 (en) * | 2003-05-06 | 2004-11-25 | Novozymes North America, Inc. | Use of hemicellulase composition in mechanical pulp production |
US8268583B2 (en) | 2003-12-10 | 2012-09-18 | Novozymes A/S | Cell with improved secretion mediated by MrgA protein or homologue |
US20060121042A1 (en) | 2004-10-27 | 2006-06-08 | Medimmune, Inc. | Modulation of antibody specificity by tailoring the affinity to cognate antigens |
JP5226958B2 (ja) * | 2007-02-22 | 2013-07-03 | 花王株式会社 | 組換え微生物 |
WO2012127001A1 (en) | 2011-03-23 | 2012-09-27 | Novozymes A/S | Methods for producing secreted polypeptides |
WO2012127002A1 (en) | 2011-03-23 | 2012-09-27 | Novozymes A/S | Sweet-tasting polypeptide from gram-positive bacteria |
EP2546272A1 (en) | 2011-07-15 | 2013-01-16 | Ticona GmbH | Process for producing oxymethylene polymers |
CN104845923B (zh) * | 2014-02-14 | 2018-03-23 | 中国科学院微生物研究所 | 生产l‑组氨酸的方法及其专用重组菌 |
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EP3918086A1 (en) | 2019-01-30 | 2021-12-08 | Novozymes A/S | Cognate foldase co-expression |
US20220170003A1 (en) | 2019-03-08 | 2022-06-02 | Novozymes A/S | Means And Methods For Improving Protease Expression |
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US4711843A (en) * | 1980-12-31 | 1987-12-08 | Cetus Corporation | Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis |
US4994379A (en) * | 1983-03-09 | 1991-02-19 | Cetus Corporation | Modified signal peptides |
US5017477A (en) * | 1985-10-25 | 1991-05-21 | Biotechnica International, Inc. | Enhancing DNA sequencies derived from the sacQ gene |
US4879213A (en) * | 1986-12-05 | 1989-11-07 | Scripps Clinic And Research Foundation | Synthetic polypeptides and antibodies related to Epstein-Barr virus early antigen-diffuse |
US4965197A (en) * | 1987-06-12 | 1990-10-23 | Massachusetts Institute Of Technology | Coryneform expression and secretion system |
-
1994
- 1994-02-25 DK DK94908357T patent/DK0686195T3/da active
- 1994-02-25 JP JP6518691A patent/JPH08507207A/ja not_active Withdrawn
- 1994-02-25 EP EP94908357A patent/EP0686195B1/en not_active Expired - Lifetime
- 1994-02-25 AT AT94908357T patent/ATE335083T1/de not_active IP Right Cessation
- 1994-02-25 AU AU61426/94A patent/AU6142694A/en not_active Abandoned
- 1994-02-25 WO PCT/FI1994/000072 patent/WO1994019471A1/en active IP Right Grant
- 1994-02-25 US US08/507,391 patent/US5780261A/en not_active Expired - Lifetime
- 1994-02-25 CN CN94191732A patent/CN1080307C/zh not_active Expired - Lifetime
- 1994-02-25 DE DE69434807T patent/DE69434807T2/de not_active Expired - Lifetime
-
2004
- 2004-09-02 JP JP2004256168A patent/JP4202985B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP2004350691A (ja) | 2004-12-16 |
US5780261A (en) | 1998-07-14 |
DE69434807D1 (de) | 2006-09-14 |
AU6142694A (en) | 1994-09-14 |
DK0686195T3 (da) | 2006-11-27 |
DE69434807T2 (de) | 2007-03-29 |
CN1080307C (zh) | 2002-03-06 |
ATE335083T1 (de) | 2006-08-15 |
CN1120853A (zh) | 1996-04-17 |
EP0686195A1 (en) | 1995-12-13 |
JP4202985B2 (ja) | 2008-12-24 |
WO1994019471A1 (en) | 1994-09-01 |
EP0686195B1 (en) | 2006-08-02 |
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