JPH08503994A - 新規化合物を用いた粒子内における蛍光エネルギー伝達および分子内エネルギー伝達 - Google Patents
新規化合物を用いた粒子内における蛍光エネルギー伝達および分子内エネルギー伝達Info
- Publication number
- JPH08503994A JPH08503994A JP7509970A JP50997095A JPH08503994A JP H08503994 A JPH08503994 A JP H08503994A JP 7509970 A JP7509970 A JP 7509970A JP 50997095 A JP50997095 A JP 50997095A JP H08503994 A JPH08503994 A JP H08503994A
- Authority
- JP
- Japan
- Prior art keywords
- component
- particles
- dye
- dyes
- energy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 229920006284 nylon film Polymers 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 1
- AICOOMRHRUFYCM-ZRRPKQBOSA-N oxazine, 1 Chemical compound C([C@@H]1[C@H](C(C[C@]2(C)[C@@H]([C@H](C)N(C)C)[C@H](O)C[C@]21C)=O)CC1=CC2)C[C@H]1[C@@]1(C)[C@H]2N=C(C(C)C)OC1 AICOOMRHRUFYCM-ZRRPKQBOSA-N 0.000 description 1
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- WCNLCIJMFAJCPX-UHFFFAOYSA-N pethidine hydrochloride Chemical compound Cl.C=1C=CC=CC=1C1(C(=O)OCC)CCN(C)CC1 WCNLCIJMFAJCPX-UHFFFAOYSA-N 0.000 description 1
- 125000005498 phthalate group Chemical class 0.000 description 1
- 229920006391 phthalonitrile polymer Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 229960003975 potassium Drugs 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
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- RIRUMIYJOLQEDW-UHFFFAOYSA-N quinoline dihydrate Chemical compound N1=CC=CC2=CC=CC=C12.O.O RIRUMIYJOLQEDW-UHFFFAOYSA-N 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
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- NURIJECXIAPSAM-UHFFFAOYSA-N silicon phthalocyanine dichloride Chemical compound N1=C(C2=CC=CC=C2C2=NC=3C4=CC=CC=C4C(=N4)N=3)N2[Si](Cl)(Cl)N2C4=C(C=CC=C3)C3=C2N=C2C3=CC=CC=C3C1=N2 NURIJECXIAPSAM-UHFFFAOYSA-N 0.000 description 1
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- FWPXRSGLRILKNV-UHFFFAOYSA-N trihexyl(trihexylsilyloxy)silane Chemical compound CCCCCC[Si](CCCCCC)(CCCCCC)O[Si](CCCCCC)(CCCCCC)CCCCCC FWPXRSGLRILKNV-UHFFFAOYSA-N 0.000 description 1
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09B47/04—Phthalocyanines abbreviation: Pc
- C09B47/08—Preparation from other phthalocyanine compounds, e.g. cobaltphthalocyanineamine complex
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- C09B67/0097—Dye preparations of special physical nature; Tablets, films, extrusion, microcapsules, sheets, pads, bags with dyes
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.粒子内に、第1成分としてエネルギーのドナーを、第2成分としてエネル ギーのアクセプターを、互いがエネルギー交換距離にある位置に含有しており、 該2つの成分が50nm以上のストークスシフトを有し、該粒子の表面に上にタ ンパク質、ポリペプチド、核酸、ヌクレオチド、またはリガンドアナログを含有 するタンパク質が結合している粒子。 2.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光色 素を、互いがエネルギー交換距離にある位置に含有しており、該第1成分がフタ ロシアニンであり、該2つの成分が50nm以上のストークスシフトを有してい る粒子。 3.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光色 素を、互いがエネルギー交換距離にある位置に含有しており、該第2成分がフタ ロシアニンであり、該2つの成分が50nm以上のストークスシフトを有してい る粒子。 4.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光色 素を、互いの距離がエネルギー交換距離にある位置に含有しており、該第1成分 がナフタロシアニンであり、該2つの成分が50nm以上のストークスシフトを 有している粒子。 5.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光色 素を、互いがエネルギー交換距離にある位置に含有しており、該第2成分がナフ タロシアニンであり、該2つの成分が50nm以上のストークスシフトを有して いる粒子。 6.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光色 素を、互いがエネルギー交換距離にある位置に含有しており、第1成分がフタロ シアニンであり、第2成分がナフタロシアニンであり、該2つの成分が50nm 以上のストークスシフトを有している粒子。 8.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光色 素を、互いがエネルギー交換距離にある位置に含有しており、第1成分がスチリ ルであり、第2成分がナフタロシアニンであり、該2つの成分が50nm以上の ストークスシフトを有している粒子。 10.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、第1成分がフェ ニルブタジエニルであり、第2成分がナフタロシアニンであり、該2つの成分が 50nm以上のストークスシフトを有している粒子。 12.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、第1成分がフェ ニルヘキサトリエニルであり、第2成分がナフタロシアニンであり、該2つの成 分が50nm以上のストークスシフトを有している粒子。 14.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、第1成分がポル フィンであり、第2成分がナフタロシアニンであり、該2つの成分が50nm以 上のストークスシフトを有している粒子。 16.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、第1成分がカル ボシアニン色素であり、第2成分がナフタロシアニンであり、該2つの成分が5 0nm以上のストークスシフトを有している粒子。 37.粒子内に、第1成分としてエネルギーのドナーを有し、さらに互いがエ ネルギー交換距離にある位置に2種類の蛍光色素を有する粒子であって、該第1 成分がトランス-4-[4-(ジブチルアミノ)スチリル]-1-メチルピリジンで あり、該2種類の色素がシリコンフタロシアニンビス(ジメチルペンタフルオロ フェニルシリルオキシド)およびシリコンフタロシアニンビス(ジメチルビニル シリルオキシド)からなる群から抽出され、該エネルギーのドナーと2種類の色 素のストークスシフトが50nm以上である粒子。 38.粒子内に、第1成分としてエネルギーのドナーを有し、さらに互いがエ ネルギー交換距離にある位置に3種類の蛍光色素を有する粒子であって、該第1 成分がトランス-4-[4-(ジブチルアミノ)スチリル]-1-メチルピリジンで あり、該3種類の色素がシリコンフタロシアニンビス(ジメチルペンタフルオロ フェニルシリルオキシド)、シリコンフタロシアニンビス(ジメチルビニルシリ ルオキシド)およびシリコンフタロシアニンビス(ジメチルビニルシリルオキシ ド)からなる群から抽出され、該エネルギーのドナーと3種類の色素のストーク スシフトが50nm以上である粒子。 43.粒子内に、第1成分としてエネルギーのドナーを、第2成分として2種 類の蛍光色素を、互いがエネルギー交換距離にある位置に含有しており、第1成 分が1,1’-ジヘキシル-3,3,3’,3-テトラメチルインドジカルボシア ニンであり、該2種類の色素はシリコン2,3-ナフタロシアニンビス(ジメチ ルビニルシリルオキシド)およびシリコンナフタロシアニンビス(ジメチルエチ ルマレイミドシリルオキシド)からなる群から抽出され、該エネルギードナーと 2種類の蛍光色素のストークスシフトが50nm以上である粒子。 44,粒子内に、第1成分としてエネルギーのドナーを、第2成分として2種 の蛍光色素を、互いがエネルギー交換距離にある位置に含有しており、該第1成 分が1,1’-ジヘキシル-3,3,3’,3’-テトラメチルインドジカルボシ アニンの塩であり、該2種の色素がシリコン2,3-ナフタロシアニンビス(ジ メチルビニルシリルオキシド)およびシリコンフタロシアニンビス(ジメチルエ チルマレイミドシリルオキシド)からなる群から抽出され、該エネルギーのドナ ーと2種類の色素のストークスシフトが50nm以上である粒子。 57.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、該第1成分が( E,E)-3,5-ビス-(4-フェニル-1,3-ブタジエニル)-4,4-ジフルオ ロ-4-ボラ-3a,4a-ジアゾ-s-インダセンであり、該第2成分がシリコン2 ,3-ナフタロシアニンビス(ジメチルヘキシルビニルシリルオキシド)であり 、該2つの成分が50nm以上のストークスシフトを有している粒子。 64.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、該第1成分が( E,E)-3,5-ビス-(4-フェニル-1,3-ブタジエニル)-4,4-ジフルオ ロ-4-ボラ-3a,4a-ジアゾ-s-インダセンであり、該第2成分がシリコン2 ,3-ナフタロシアニンビス(ジメチルビニルシリルオキシド)であり、該2つ の成分が50nm以上のストークスシフトを有している粒子。 72.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、該第1成分がシ リコンフタロシアニンビス(マレイミド-フルオロセイン)(FFT化合物)で あり、該第2成分がシリコンフタロシアニンビス(マレイミド-フルオロセイン )(FET化合物)および該2つの成分が50nm以上のストークスシフトを有 している粒子。 77.粒子内に、第1成分としてエネルギーのドナーを、第2成分として蛍光 色素を、互いがエネルギー交換距離にある位置に含有しており、該第1成分が( E,E)-3,5-ビス-(4-フェニル-1,3-ブタジエニル)-4,4-ジフルオ ロ-4-ボラ-3a,4a-ジアゾ-s-インダセンであり、該第2成分がシリコン2 ,3-ナフタロシアニンビス(ジメチルヘキシルビニルシリルオキシド)であり 、該2つの成分が50nm以上のストークスシフトを有している粒子。 86.粒子内に、第1成分としてエネルギーのドナーを、第2成分として2種 類の蛍光色素を、互いがエネルギー交換距離にある位置に含有しており、該第1 成分が(E,E)-3,5-ビス-(4-フェニル-1,3-ブタジエニル)-4,4- ジフルオロ-4-ボラ-3a,4a-ジアゾ-s-インダセンであり、該第2成分の2 種の色素がシリコン2,3-ナフタロシアニンビス(ジメチルヘキシルビニルシ リルオキシド)およびシリコンオクタエトキシ2,3-ナフタロシアニンビス( ジメチルヘキシルビニルシリルオキシド)からなる群から抽出され、該エネルギ ードナーと2種類の色素のストークスシフトが50nm以上である粒子。 101.粒子内に、第1成分としてエネルギーのドナーを、第2成分として2 種類の蛍光色素を、互いがエネルギー交換距離にある位置に含有しており、該第 1成分が(E,E)-3,5-ビス-(4-フェニル-1,3-ブタジエニル)-4, 4-ジフルオロ-4-ボラ-3a,4a-ジアゾ-s-インダセンであり、該第2成分 の2種の色素がシリコン2,3-ナフタロシアニンビス(ジメチルヘキシルビニ ルシリルオキシド)および5,5’-ジクロロ-1,1’-ジフェニルアミノ-3, 3’-ジエチル-10,12-エチレンチアトリカルボシアニンの塩からなる群か ら抽出され、該エネルギードナーと2種類の色素のストークスシフトが50nm 以上である粒子。 104.粒子内に、第1成分としてエネルギーのドナーを、第2成分として2 種類の蛍光色素を、互いがエネルギー交換距離にある位置に含有しており、該第 1成分が(E,E)-3,5-ビス-(4-フェニル-1,3-ブタジエニル)-4, 4-ジフルオロ-4-ボラ-3a,4a-ジアゾ-s-インダセンであり、該第2成分 の2種の色素がシリコン2,3-ナフタロシアニンビス(ジメチルヘキシルビニ ルシリルオキシド)およびシリコン2,3-ナフタロシアニンビス(ジメチルペ ンタフルオロフェニルシリルオキシド)からなる群から抽出され、該エネルギー のドナーと2種類の色素が50nm以上のストークスシフトを有している粒子。 105.粒子がラテックス粒子である請求項1〜104いずれかに記載の粒 子。 106.粒子内に、ほぼ同一の励起波長と放射波長を有する2またはそれ以上 の色素分子を添加し、これによって蛍光消光が減少され、蛍光強度が該色素分子 の組み合わせにより増加されるよう改良された改良粒子。 107.粒子内において第2成分とほぼ同じ励起および放射波長を示す少なく とも1種類の蛍光色素を第3成分としてさらに含有し、これによって消光が減少 され、該第2および第3の追加成分の組み合わせによって蛍光強度が増加される 、請求項1〜36、39〜42、45〜85、87〜100または102〜10 3のいずれかに記載の粒子。 106.少なくとも1のさらなる蛍光色素を第4成分として含有し、該第4成 分が粒子内で該2つのアクセプター色素とほぼ同じ励起および放射波長を示し、 これによって消光が減少され、該2種類の色素および該追加成分の組み合わせに よって蛍光強度が増強される、請求項37、43、44、86、101または1 04記載の粒子。 107.少なくとも1のさらなる蛍光色素を第3成分として含有し、該第3成 分が粒子内で該第2成分とほぼ同じ励起および放射波長を示し、これによって消 光が減少し、該第2成分と該追加成分の組み合わせにより蛍光強度が増強される 、請求項1〜36、39〜42、43〜85、87〜100または102〜10 3いずれかに記載のラテックス粒子。 108.少なくとも1のさらなる蛍光色素を第4成分として含有し、該第4成 分が粒子内で該2種のアクセプター色素とほぼ同じ励起および放射波長を示し、 これによって消光が減少され、蛍光強度が該2種のアクセプター色素と該追加成 分の組み合わせにより増強される、請求項37、43、44、86、101また は104記載のラテックス粒子。 109.請求項1〜104いずれかに記載の粒子を用いて反応混合物内の標的 リガンドを検索する、診断アッセイ。 110.a)少なくとも1の所望の励起ピークを有する出発ドナー色素および 少なくとも1の所望の放射ピークを有する最終アクセプター色素を含有する一 連の色素を、該一連の色素中の各色素のスペクトルが、励起エネルギーを最終の アクセプター色素へ伝達する有意なエネルギー伝達が可能であるよう重複し、該 微粒子の励起波長が600ナノメートル以上であり、ストークスシフトが50ナ ノメートル以上となるように選択する;そしてb)該一連の色素を微粒子内へラ ンダムに取り込ませる:工程を含む方法により製造される、蛍光微粒子。 111.620nmから750nmの間に励起ピークを有し、650nmから 850nmの間に放射ピークを有する請求項110記載の微粒子。 112.650nmから900nmの間に励起ピークを有し、800nmから 1000nmの間に放射ピークを有する請求項110記載の微粒子。 113.ラテックス、シリカ、アルミナ、リポソーム及びコロイドからなる群 から選択される、請求項111記載の微粒子。 114.ラテックス、シリカ、アルミナ、リポソーム及びコロイドからなる群 から選択される、請求項112記載の微粒子。 115.A)1)所望の励起ピークを有する少なくとも1のドナーサブユニッ ト;および2)所望の放射ピークを有する少なくとも1のアクセプターサブユニ ットを有し、該ドナーサブユニットからアクセプターサブユニットへの分子内エ ネルギー伝達が可能である;少なくとも1のハイブリッドフタロシアニン誘導体 を選択する、B)該ハイブリッドフタロシアニン誘導体を微粒子内へランダムに 取り込ませる:工程を含む方法にて作成される微粒子。 116.ラテックス、シリカ、アルミナ、リポソーム及びコロイドからなる群 から選択される、請求項115記載の微粒子。 117.A)(1)所望の励起ピークを有する少なくとも1のドナーサブユニ ット;(2)所望の放射ピークを有する少なくとも1のアクセプターサブユニッ ト;および(3)少なくとも1の電子伝達サブユニットを有し、該ドナーサブユ ニットからアクセプターサブユニットへの分子内エネルギー伝達が可能である; 少なくとも1のハイブリッドフタロシアニン誘導体を選択する、B)該ハイブリ ッドフタロシアニン誘導体を微粒子内へランダムに取り込ませる:工程を含む方 法にて作成される微粒子。 118.ラテックス、シリカ、アルミナ、リポソームおよびコロイドからなる 群から選択される、請求項117記載の微粒子。 119.A)(1)所望の励起ピークを有する少なくとも1のドナーサブユニ ット;(2)所望の放射ピークを有する少なくとも1のアクセプターサブユニッ ト;および(3)ハイブリッドフタロシアニン内の金属に共有結合している少な くとも1の軸性配位子を有し、該ドナーサブユニットからアクセプターサブユニ ットへの分子内エネルギー伝達が可能である;少なくとも1の金属含有ハイブリ ッドフタロシアニン誘導体を選択する、B)該ハイブリッドフタロシアニン誘導 体を微粒子内へ取り込ませる:工程を含む方法にて作成される微粒子。 120.ラテックス、シリカ、アルミナ、リポソームおよびコロイドからなる 群から選択される、請求項119記載の微粒子。 121.A)ハイブリッドフタロシアニン内の金属に共有結合している少なく とも1の軸性配位子を有する、少なくとも1の金属含有ハイブリッドフタロシア ニン誘導体を選択する;B)該ハイブリッドフタロシアニン誘導体を微粒子内へ 取り込ませる;工程を含む方法にて作成される微粒子。 122.ラテックス、シリカ、アルミナ、リポソームおよびコロイドからなる 群から選択される、請求項121記載の微粒子。 123.ハイブリッドフタロシアニン誘導体内の金属に共有結合している少な くとも1の軸性配位子を含有する、金属含有ハイブリッドフタロシアニン誘導体 。 124.A)(1)所望の励起ピークを有する少なくとも1のドナーサブユニ ット;(2)所望の放射ピークを有する少なくとも1のアクセプターサブユニッ ト;(3)少なくとも1の電子伝達サブユニット;および(4)ハイブリッドフ タロシアニン内の金属に共有結合している少なくとも1の軸性配位子を有し、該 ドナーサブユニットからアクセプターサブユニットへの分子内エネルギー伝達が 可能である;少なくとも1の金属含有ハイブリッドフタロシアニン誘導体を選択 する、B)該フタロシアニン誘導体を微粒子内へ取り込ませる:工程を含む方法 にて作成される微粒子。 125.ラテックス、シリカ、アルミナ、リポソームおよびコロイドからなる 群から選択される、請求項124記載の微粒子。 126.フタロシアニン誘導体内の金属に共有結合している少なくとも1の軸 性リガンドを含有する、金属含有フタロシアニン誘導体を含む微粒子。 127.ハイブリッドフタロシアニン誘導体内の金属に共有結合している少な くとも1の軸性リガンドを含有する、金属含有ハイブリッドフタロシアニン誘導 体を含む微粒子。 134.A)色素系中に、所望の励起ピークを有する少なくとも1の出発のド ナーサブユニット、および所望の、互いに同一もしくは非常に近接している放射 ピークを有する2またはそれ以上の最終アクセプター色素を含有し、該色素系の 各色素が励起エネルギーを最終のアクセプター色素へ伝達する有意なエネルギー 伝達を十分行えるようなスペクトルの重複を有するように、一連の色素系を選択 する;そしてB)該一連の色素系を微粒子内へランダムに取り込ませて最小の蛍 光消光と最大の蛍光強度を示す改良された粒子となるようにする:工程を含む製 法にて製造される、改良された蛍光微粒子。 135.該放射ピークが互いに10nmの範囲内にある、請求項134記載の 改良された微粒子。 136.該一連の色素系に5個までの異なる色素を含有する請求項134記載 の改良された微粒子。 137.該一連の色素系に10個までの異なる色素を含有する請求項134記 載の改良された微粒子。 138.a)カルボシアニン色素およびエテニル置換ジピロロメテンボロンジ フルオロ色素からなる群から選択され、所望の励起ピークを有する少なくとも1 の出発ドナー色素、および、フタロシアニン類からなる群から選択され、所望の 放射ピークを有する少なくとも1の最終アクセプター色素を含有し、色素系中の 各色素が励起エネルギーを最終アクセプター色素へ伝達するのに十分なスペクト ルの重複を有しており、ストークスシフトが50ナノメートル以上である色素 系を選択し;b)該一連の色素系を微粒子内にランダムに取り込ませる:工程を 含む方法で製造される蛍光微粒子。 139.A)所望の励起ピークを有する少なくとも1の出発ドナー色素、およ び所望の放射ピークを有する少なくとも1の最終アクセプター色素を含有し、色 素系中の各色素のスペクトルが励起エネルギーを最終アクセプター色素へ伝達す るのに十分であるよう重複しており、該微粒子の励起波長が、試料の吸収が入射 光の約10%以下である領域内であり、試料の蛍光の寄与が、バックグラウンド 信号の約10%以下であるような一連の色素系を選択し、B)該色素系をランダ ムに微粒子内に取り込ませる:工程を含む方法で製造される、少なくとも1のア ナライトを、該アナライトを含有することが予測される試料内から検出するため の蛍光微粒子。 140.試料が生の血清、生の尿及び生の血漿からなる群から選択される、請 求項139記載の微粒子。 141.A)励起波長が、試料の吸収が入射光の約10%以下であり、放射が 試料の蛍光バックグラウンド信号に対して10%以下となる領域であるよう選択 された所望の励起ピークおよび放射ピークを有する、ハイブリッドフタロシアニ ン誘導体を選択し;B)該一連の色素を微粒子内にランダムに取り込ませる工程 を含む方法で製造される、試料中の少なくとも1のアナライトを検出するための 蛍光微粒子。 142.該試料が生の血清、生の尿および生の血漿からなる群から選択される 、請求項141記載の微粒子。 143.シリコン[ジ(1,6−ジフェニルナフタロシアニン)]ジフタロシ アニンビス(ジメチルヘキシルビニルシリルオキシド)。 144.シリコン[ジ(1,6−ジフェニルナフタロシアニン)]テトラフル オロフタロシアニンフタロシアニンビス(ジメチルヘキシルビニルシリルオキシ ド)。 145.シリコン[ジ(1,6−ジフェニルナフタロシアニン)]テトラフル オロフタロシアニンフタロシアニンビス(ジメチルペンタフルオロフェニルシ リルオキシド)。 146.シリコン[ジ(1,6−ジフェニルナフタロシアニン)]ジフタロシ アニンビス(ジメチルペンタフルオロフェニルシリルオキシド)。 147.シリコン[ジ(1,6−ジフェニルナフタロシアニン)]ジ(第3ブ チル−フタロシアニン)ビス(ジメチルヘキシルビニルシリルオキシド)。
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US08/274,534 US6238931B1 (en) | 1993-09-24 | 1994-07-12 | Fluorescence energy transfer in particles |
US08/274,534 | 1994-07-12 | ||
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- 1994-09-23 US US08/311,098 patent/US5763189A/en not_active Expired - Lifetime
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Also Published As
Publication number | Publication date |
---|---|
JP3773949B2 (ja) | 2006-05-10 |
DE69525186D1 (de) | 2002-03-14 |
EP0670041A1 (en) | 1995-09-06 |
CA2149419C (en) | 2007-05-15 |
EP0670041A4 (en) | 1997-11-05 |
US5763189A (en) | 1998-06-09 |
EP0670041B1 (en) | 2002-01-30 |
WO1995008772A1 (en) | 1995-03-30 |
CA2149419A1 (en) | 1995-03-30 |
DE69525186T2 (de) | 2002-10-31 |
ATE212721T1 (de) | 2002-02-15 |
AU8011294A (en) | 1995-04-10 |
US6238931B1 (en) | 2001-05-29 |
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