JPH05502797A - コリネバクテリアのインテグロン、該インテグロンによるコリネバクテリアの形質転換方法、及び得られたコリネバクテリア - Google Patents

コリネバクテリアのインテグロン、該インテグロンによるコリネバクテリアの形質転換方法、及び得られたコリネバクテリア

Info

Publication number
JPH05502797A
JPH05502797A JP3514354A JP51435491A JPH05502797A JP H05502797 A JPH05502797 A JP H05502797A JP 3514354 A JP3514354 A JP 3514354A JP 51435491 A JP51435491 A JP 51435491A JP H05502797 A JPH05502797 A JP H05502797A
Authority
JP
Japan
Prior art keywords
integron
corynebacterium
sequence
gene
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3514354A
Other languages
English (en)
Japanese (ja)
Inventor
ギヨンバルシュ,アルメル、アンドレ、イブ
レイェ,アルバラド、オスカル、ジュリオ
ラバール,ジャン、クリスチャン、ジョスラン
ボナミ,セリーヌ、アンヌ、マリー
レブロン,ジェラール、ルイ、アンドレ
Original Assignee
サントル、ナショナル、ド、ラ、ルシェルシュ、シアンティフィク(セエヌエルエス)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by サントル、ナショナル、ド、ラ、ルシェルシュ、シアンティフィク(セエヌエルエス) filed Critical サントル、ナショナル、ド、ラ、ルシェルシュ、シアンティフィク(セエヌエルエス)
Publication of JPH05502797A publication Critical patent/JPH05502797A/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/60Vectors containing traps for, e.g. exons, promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/90Vectors containing a transposable element

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP3514354A 1990-08-08 1991-08-08 コリネバクテリアのインテグロン、該インテグロンによるコリネバクテリアの形質転換方法、及び得られたコリネバクテリア Pending JPH05502797A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9010126A FR2665711B1 (fr) 1990-08-08 1990-08-08 Integron de corynebacterie, procede de transformation d'une corynebacterie par ledit integron et corynebacterie obtenue.
FR90/10126 1990-08-08

Publications (1)

Publication Number Publication Date
JPH05502797A true JPH05502797A (ja) 1993-05-20

Family

ID=9399530

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3514354A Pending JPH05502797A (ja) 1990-08-08 1991-08-08 コリネバクテリアのインテグロン、該インテグロンによるコリネバクテリアの形質転換方法、及び得られたコリネバクテリア

Country Status (17)

Country Link
EP (1) EP0495078A1 (de)
JP (1) JPH05502797A (de)
KR (1) KR920702423A (de)
CN (1) CN1061624A (de)
AU (1) AU646886B2 (de)
BR (1) BR9105857A (de)
CA (1) CA2067240A1 (de)
FI (1) FI921527A0 (de)
FR (1) FR2665711B1 (de)
HU (1) HUT63656A (de)
IE (1) IE912791A1 (de)
IL (1) IL99126A0 (de)
MX (1) MX9100560A (de)
NZ (1) NZ239309A (de)
PT (1) PT98602B (de)
WO (1) WO1992002627A1 (de)
ZA (1) ZA916216B (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69331255T2 (de) * 1992-03-11 2002-08-22 Ajinomoto Kk Transponierbares-element aus dem bakterium vom genus brevibakterium
EP0756007A3 (de) * 1995-06-30 1997-10-29 Ajinomoto Kk Genvermerungsverfahren mit kunstlichen Transposon
US5989914A (en) * 1996-06-03 1999-11-23 Universite Laval Integration cassette for improvement of transgenesis in eukaryotes
JP4035855B2 (ja) 1996-06-05 2008-01-23 味の素株式会社 L−リジンの製造法
DE10046870A1 (de) * 2000-09-20 2002-03-28 Basf Ag Verfahren zur Veränderung des Genoms von Corynebakterien
DE102006032634A1 (de) 2006-07-13 2008-01-17 Evonik Degussa Gmbh Verfahren zur Herstellung von L-Aminosäuren
KR100830826B1 (ko) 2007-01-24 2008-05-19 씨제이제일제당 (주) 코리네박테리아를 이용하여 글리세롤을 포함한탄소원으로부터 발효산물을 생산하는 방법
CN101503670B (zh) * 2008-02-04 2011-12-14 复旦大学附属华山医院 一种含有整合子的工程菌株
KR101126041B1 (ko) 2008-04-10 2012-03-19 씨제이제일제당 (주) 트랜스포존을 이용한 형질전환용 벡터, 상기 벡터로형질전환된 미생물 및 이를 이용한 l-라이신 생산방법
US8932861B2 (en) 2008-04-10 2015-01-13 Cj Cheiljedang Corporation Transformation vector comprising transposon, microorganisms transformed with the vector, and method for producing L-lysine using the microorganism
EP2479279A1 (de) 2011-01-20 2012-07-25 Evonik Degussa GmbH Verfahren zur fermentativen Herstellung schwefelhaltiger Aminosäuren
EP2628792A1 (de) 2012-02-17 2013-08-21 Evonik Industries AG Zelle mit verringerter ppGppase-Aktivität
PL2700715T3 (pl) 2012-08-20 2019-03-29 Evonik Degussa Gmbh Sposób fermentacyjnego wytwarzania L-aminokwasów z zastosowaniem ulepszonych szczepów z rodziny Enterobacteriaceae

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57183799A (en) * 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
IL67510A (en) * 1981-12-17 1988-08-31 Kyowa Hakko Kogyo Kk Recombinant vector plasmids autonomously replicable in microorganisms belonging to the genus corynebacterium or brevibacterium and process for the production thereof
JPS59205983A (ja) * 1983-04-28 1984-11-21 ジエネツクス・コ−ポレイシヨン 異種遺伝子を原核微生物で発現させる方法
GB8529275D0 (en) * 1985-11-28 1986-01-02 Whitbread & Co Plc Dna recombination
ES2058133T3 (es) * 1986-12-26 1994-11-01 Takeda Chemical Industries Ltd Adn que codifica una imp-deshidrogenasa inactivada.
FR2615527B1 (fr) * 1987-05-22 1989-08-18 Lesaffre Soc Ind Procede d'integration d'une sequence connue d'adn dans les levures ascosporogenes, vecteurs mis en oeuvre et nouvelles souches de levures
NL8701450A (nl) * 1987-06-22 1989-01-16 Solvay Werkwijze voor het transformeren van cellen.
DK639689D0 (da) * 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler

Also Published As

Publication number Publication date
WO1992002627A1 (fr) 1992-02-20
AU646886B2 (en) 1994-03-10
PT98602B (pt) 1999-01-29
AU8442391A (en) 1992-03-02
IE912791A1 (en) 1992-02-12
EP0495078A1 (de) 1992-07-22
FI921527A (fi) 1992-04-07
ZA916216B (en) 1992-04-29
CN1061624A (zh) 1992-06-03
KR920702423A (ko) 1992-09-04
HUT63656A (en) 1993-09-28
IL99126A0 (en) 1992-07-15
PT98602A (pt) 1992-08-31
BR9105857A (pt) 1992-09-29
MX9100560A (es) 1992-04-01
CA2067240A1 (fr) 1992-02-09
FR2665711B1 (fr) 1993-08-13
FR2665711A1 (fr) 1992-02-14
NZ239309A (en) 1993-07-27
FI921527A0 (fi) 1992-04-07

Similar Documents

Publication Publication Date Title
Camilli et al. Insertional mutagenesis of Listeria monocytogenes with a novel Tn917 derivative that allows direct cloning of DNA flanking transposon insertions
Quandt et al. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria
EP0805867B1 (de) Dna-integration durch transposition
US4713337A (en) Method for deletion of a gene from a bacteria
US5773267A (en) D29 shuttle phasmids and uses thereof
Casadaban Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.
JP5732496B2 (ja) Dna分子及び方法
JPH05502797A (ja) コリネバクテリアのインテグロン、該インテグロンによるコリネバクテリアの形質転換方法、及び得られたコリネバクテリア
Lee et al. Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis
DeFranco et al. Molecular cloning of chemotaxis genes and overproduction of gene products in the bacterial sensing system
Stephens et al. Genetics of gliding motility in Myxococcus xanthus: molecular cloning of the mgl locus
Russell et al. Exchange of chromosomal and plasmid alleles in Escherichia coli by selection for loss of a dominant antibiotic sensitivity marker
Labarre et al. Gene replacement, integration, and amplification at the gdhA locus of Corynebacterium glutamicum
Wild et al. A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50-to 100-kb genomic fragments for direct DNA sequencing
DE60023501T2 (de) Einstufiger allel-austausch in mykobakterium mittels konditionell transduzierendem phagen
US4725535A (en) Promoter probe vectors
Young et al. Stability of reiterated sequences in the Bacillus subtilis chromosome
Ainsa et al. Construction of a family of Mycobacterium/Escherichia coli shuttle vectors derived from pAL5000 and pACYC184: their use for cloning an antibiotic-resistance gene from Mycobacterium fortuitum
JPS63233790A (ja) 細菌中に含まれるプラスミドの安定化方法及びこれにより得られる菌株
Lee et al. Genetic characterization of the araE gene in Salmonella typhimurium LT2
DeLong et al. Trans-acting transposase mutant from Tn5.
US4784952A (en) Conferred susceptibility to lambda phage in non-coliform procaryotic hosts
Friedberg et al. A new hybrid plasmid capable of transforming Escherichia coli and Anacystis nidulans
EP0183469A1 (de) Phagenresistentes E. coli-Bakterium und Verfahren zu seiner Herstellung
AU649984B2 (en) Transformation of bacillus thuringiensis and production of a mutant B.t. endotoxin