JPH0522508B2 - - Google Patents

Info

Publication number
JPH0522508B2
JPH0522508B2 JP61233285A JP23328586A JPH0522508B2 JP H0522508 B2 JPH0522508 B2 JP H0522508B2 JP 61233285 A JP61233285 A JP 61233285A JP 23328586 A JP23328586 A JP 23328586A JP H0522508 B2 JPH0522508 B2 JP H0522508B2
Authority
JP
Japan
Prior art keywords
bacillus subtilis
protease
strain
gene
extracellular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP61233285A
Other languages
English (en)
Japanese (ja)
Other versions
JPS6387975A (ja
Inventor
Yoshio Furuya
Masaru Pponjo
Akira Nakayama
Koichi Kawamura
Hiroaki Shimada
Izumi Mita
Akiko Akaoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP61233285A priority Critical patent/JPS6387975A/ja
Priority to GB8723033A priority patent/GB2198439B/en
Priority to FR8713672A priority patent/FR2604726B1/fr
Publication of JPS6387975A publication Critical patent/JPS6387975A/ja
Priority to US07/553,356 priority patent/US5084383A/en
Publication of JPH0522508B2 publication Critical patent/JPH0522508B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP61233285A 1986-10-02 1986-10-02 枯草菌菌株 Granted JPS6387975A (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP61233285A JPS6387975A (ja) 1986-10-02 1986-10-02 枯草菌菌株
GB8723033A GB2198439B (en) 1986-10-02 1987-10-01 Bacillus subtilis strain of reduced extracellular protease activity; secreting proteins using the strain.
FR8713672A FR2604726B1 (fr) 1986-10-02 1987-10-02 Souche de bacillus subtilis dont les activites de protease extra-cellulaire sont reduites, procede pour obtenir la souche et procede pour faire secreter des proteines en utilisant la souche
US07/553,356 US5084383A (en) 1986-10-02 1990-07-18 Bacillus subtilis strain whose extracellular protease activities are reduced, method for obtaining the strain and method for secreting proteins by using the strain

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61233285A JPS6387975A (ja) 1986-10-02 1986-10-02 枯草菌菌株

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP30163592A Division JP2857730B2 (ja) 1992-10-15 1992-10-15 枯草菌菌株による蛋白分泌の方法

Publications (2)

Publication Number Publication Date
JPS6387975A JPS6387975A (ja) 1988-04-19
JPH0522508B2 true JPH0522508B2 (https=) 1993-03-29

Family

ID=16952702

Family Applications (1)

Application Number Title Priority Date Filing Date
JP61233285A Granted JPS6387975A (ja) 1986-10-02 1986-10-02 枯草菌菌株

Country Status (4)

Country Link
US (1) US5084383A (https=)
JP (1) JPS6387975A (https=)
FR (1) FR2604726B1 (https=)
GB (1) GB2198439B (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6437284A (en) * 1987-07-31 1989-02-07 Md Res Bacillus subtilis having lowered protease productivity
GB8727772D0 (en) * 1987-11-27 1987-12-31 Celltech Ltd Host cells
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
WO1991002803A1 (en) * 1989-08-25 1991-03-07 Henkel Research Corporation A method of suppressing chromosomal gene expression
US5585253A (en) * 1989-11-27 1996-12-17 The Regents Of The University Of California Extracellular serine protease and a Bacillus subtilis alkaline neutral an serine protease mutant strain
CN111705050B (zh) * 2020-05-19 2022-07-22 江苏大学 一种新型嗜盐古菌胞外蛋白酶的制备方法及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711843A (en) * 1980-12-31 1987-12-08 Cetus Corporation Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis
JPS58162291A (ja) * 1982-03-23 1983-09-26 Res Dev Corp Of Japan 耐熱性プロテア−ゼの製造法
JPS59190A (ja) * 1982-06-25 1984-01-05 ヤマハ株式会社 楽譜表示装置
GB8308483D0 (en) * 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
JPH0659224B2 (ja) * 1983-09-12 1994-08-10 工業技術院長 Ｄνａ塩基配列及びそのｄνａ塩基配列を含む組換え体プラスミドの創製法
EP0149241B1 (en) * 1983-12-28 1991-03-27 Agency Of Industrial Science And Technology Dna base sequence containing regions involved in the production and secretion of a protein, recombinant dna including the whole or a part of the dna base sequence, and method of producing proteins by use of the recombinant dna
EP0151760A1 (en) * 1984-01-06 1985-08-21 Takeda Chemical Industries, Ltd. Novel DNA and use thereof
US4758512A (en) * 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
GB2171703B (en) * 1985-01-18 1989-11-15 Agency Ind Science Techn Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re

Also Published As

Publication number Publication date
JPS6387975A (ja) 1988-04-19
FR2604726B1 (fr) 1990-12-21
FR2604726A1 (fr) 1988-04-08
US5084383A (en) 1992-01-28
GB2198439B (en) 1990-10-10
GB2198439A (en) 1988-06-15
GB8723033D0 (en) 1987-11-04

Similar Documents

Publication Publication Date Title
JP2664860B2 (ja) 工業バチルス微生物種によるタンパク質又はポリペプチドの製造
CN88101681A (zh) 编码蛋白水解酶之基因的分子克隆及表达
US5780261A (en) Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria
GB2133408A (en) Method for improving the production of proteins in bacillus
JP3492935B2 (ja) プラスミドベクター
Wong et al. Use of the Bacillus subtilis subtilisin signal peptide for efficient secretion of TEM beta-lactamase during growth
JP3012026B2 (ja) 高発現ベクター及び該高発現ベクター を保有する微生物並びに該微生物を 用いる有用物質の製造法
Konishi et al. Efficient production of human α-amylase by a Bacillus brevis mutant
US5015574A (en) DNA sequence involved in gene expression and protein secretion, expression-secretion vector including the DNA sequence and the method of producing proteins by using the expression-secretion vector
JPH0522508B2 (https=)
EP0135045A2 (en) A novel plasmid and methods for its use
EP0196375A1 (en) Gene coding for signal peptides and utilization thereof
JPS6356277A (ja) 新規バチルス・ブレビス及びその利用
JP3739101B2 (ja) 変異バチルス・ブレビス菌
EP0216080B1 (en) Novel plasmids and transformants
JP2500312B2 (ja) ヒト成長ホルモンの生産法
JPH0638741A (ja) ヒラメ成長ホルモンの製造法
US5246839A (en) Secretion plasmid comprising the kilgene
WO1991002064A2 (en) Modified proteases and their use in foodstuffs
JP2857730B2 (ja) 枯草菌菌株による蛋白分泌の方法
EP0187382A2 (en) Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
CA2156425C (en) Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria
JPS61166400A (ja) 蛋白生産の方法
FI120155B (fi) Menetelmä ja järjestelmä kaupallisesti tärkeiden eksoproteiinien tehostetuksi tuottamiseksi grampositiivisissa bakteereissa
EP0316023A2 (en) Plasmids, methods for their construction, microorganisms carrying them and methods for the extracellular production of xylanase by cultivation of the microorganisms

Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term