FR2604726B1 - Souche de bacillus subtilis dont les activites de protease extra-cellulaire sont reduites, procede pour obtenir la souche et procede pour faire secreter des proteines en utilisant la souche - Google Patents
Souche de bacillus subtilis dont les activites de protease extra-cellulaire sont reduites, procede pour obtenir la souche et procede pour faire secreter des proteines en utilisant la soucheInfo
- Publication number
- FR2604726B1 FR2604726B1 FR8713672A FR8713672A FR2604726B1 FR 2604726 B1 FR2604726 B1 FR 2604726B1 FR 8713672 A FR8713672 A FR 8713672A FR 8713672 A FR8713672 A FR 8713672A FR 2604726 B1 FR2604726 B1 FR 2604726B1
- Authority
- FR
- France
- Prior art keywords
- strain
- subtil
- obtaining
- protease activities
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title 2
- 241000193830 Bacillus <bacterium> Species 0.000 title 1
- 101710089384 Extracellular protease Proteins 0.000 title 1
- 230000000694 effects Effects 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 230000002829 reductive effect Effects 0.000 title 1
- 230000003248 secreting effect Effects 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61233285A JPS6387975A (ja) | 1986-10-02 | 1986-10-02 | 枯草菌菌株 |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2604726A1 FR2604726A1 (fr) | 1988-04-08 |
FR2604726B1 true FR2604726B1 (fr) | 1990-12-21 |
Family
ID=16952702
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR8713672A Expired - Fee Related FR2604726B1 (fr) | 1986-10-02 | 1987-10-02 | Souche de bacillus subtilis dont les activites de protease extra-cellulaire sont reduites, procede pour obtenir la souche et procede pour faire secreter des proteines en utilisant la souche |
Country Status (4)
Country | Link |
---|---|
US (1) | US5084383A (fr) |
JP (1) | JPS6387975A (fr) |
FR (1) | FR2604726B1 (fr) |
GB (1) | GB2198439B (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6437284A (en) * | 1987-07-31 | 1989-02-07 | Md Res | Bacillus subtilis having lowered protease productivity |
GB8727772D0 (en) * | 1987-11-27 | 1987-12-31 | Celltech Ltd | Host cells |
CA1333777C (fr) | 1988-07-01 | 1995-01-03 | Randy M. Berka | Champignons filamenteux deficients en proteinase aspartique |
WO1991002803A1 (fr) * | 1989-08-25 | 1991-03-07 | Henkel Research Corporation | Procede de suppression de l'expression d'un gene chromosomique |
US5585253A (en) * | 1989-11-27 | 1996-12-17 | The Regents Of The University Of California | Extracellular serine protease and a Bacillus subtilis alkaline neutral an serine protease mutant strain |
CN111705050B (zh) * | 2020-05-19 | 2022-07-22 | 江苏大学 | 一种新型嗜盐古菌胞外蛋白酶的制备方法及其应用 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4711843A (en) * | 1980-12-31 | 1987-12-08 | Cetus Corporation | Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis |
JPS58162291A (ja) * | 1982-03-23 | 1983-09-26 | Res Dev Corp Of Japan | 耐熱性プロテア−ゼの製造法 |
JPS59190A (ja) * | 1982-06-25 | 1984-01-05 | ヤマハ株式会社 | 楽譜表示装置 |
GB8308483D0 (en) * | 1983-03-28 | 1983-05-05 | Health Lab Service Board | Secretion of gene products |
CA1311429C (fr) * | 1983-07-06 | 1992-12-15 | Vasantha Nagarajan | Vecteur pour polypeptides dans les bacilles |
JPH0659224B2 (ja) * | 1983-09-12 | 1994-08-10 | 工業技術院長 | Dνa塩基配列及びそのdνa塩基配列を含む組換え体プラスミドの創製法 |
EP0149241B1 (fr) * | 1983-12-28 | 1991-03-27 | Agency Of Industrial Science And Technology | Séquence de bases d'ADN contenant des régions impliquées dans la production et la sécrétion d'une protéine, ADN recombinant comprenant la totalité ou une partie de la séquence de base d'ADN et méthode pour la production de protéines utilisant cet ADN recombinant |
EP0151760A1 (fr) * | 1984-01-06 | 1985-08-21 | Takeda Chemical Industries, Ltd. | ADN et son utilisation |
US4758512A (en) * | 1984-03-06 | 1988-07-19 | President And Fellows Of Harvard College | Hosts and methods for producing recombinant products in high yields |
US4801537A (en) * | 1984-06-08 | 1989-01-31 | Genex Corporation | Vector for expression of polypeptides in bacilli |
US4828994A (en) * | 1984-09-21 | 1989-05-09 | Genex Corporation | Bacillus strains with reduced extracellular protease levels |
GB2171703B (en) * | 1985-01-18 | 1989-11-15 | Agency Ind Science Techn | Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re |
-
1986
- 1986-10-02 JP JP61233285A patent/JPS6387975A/ja active Granted
-
1987
- 1987-10-01 GB GB8723033A patent/GB2198439B/en not_active Expired - Fee Related
- 1987-10-02 FR FR8713672A patent/FR2604726B1/fr not_active Expired - Fee Related
-
1990
- 1990-07-18 US US07/553,356 patent/US5084383A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JPH0522508B2 (fr) | 1993-03-29 |
FR2604726A1 (fr) | 1988-04-08 |
GB8723033D0 (en) | 1987-11-04 |
GB2198439B (en) | 1990-10-10 |
GB2198439A (en) | 1988-06-15 |
US5084383A (en) | 1992-01-28 |
JPS6387975A (ja) | 1988-04-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse | ||
ST | Notification of lapse |