JPH049516B2 - - Google Patents
Info
- Publication number
- JPH049516B2 JPH049516B2 JP57029252A JP2925282A JPH049516B2 JP H049516 B2 JPH049516 B2 JP H049516B2 JP 57029252 A JP57029252 A JP 57029252A JP 2925282 A JP2925282 A JP 2925282A JP H049516 B2 JPH049516 B2 JP H049516B2
- Authority
- JP
- Japan
- Prior art keywords
- lipase
- oils
- fats
- immobilized
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108090001060 Lipase Proteins 0.000 claims description 57
- 102000004882 Lipase Human genes 0.000 claims description 57
- 239000004367 Lipase Substances 0.000 claims description 56
- 235000019421 lipase Nutrition 0.000 claims description 56
- 239000003921 oil Substances 0.000 claims description 31
- 239000003925 fat Substances 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 18
- 238000006460 hydrolysis reaction Methods 0.000 claims description 16
- 239000011543 agarose gel Substances 0.000 claims description 13
- 230000007062 hydrolysis Effects 0.000 claims description 11
- 230000003301 hydrolyzing effect Effects 0.000 claims description 10
- 125000000217 alkyl group Chemical group 0.000 claims description 5
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 235000019198 oils Nutrition 0.000 description 23
- 239000000499 gel Substances 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 8
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 150000004665 fatty acids Chemical class 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000003100 immobilizing effect Effects 0.000 description 4
- 239000010464 refined olive oil Substances 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
- 239000004006 olive oil Substances 0.000 description 3
- 235000008390 olive oil Nutrition 0.000 description 3
- 238000007127 saponification reaction Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 230000029936 alkylation Effects 0.000 description 2
- 238000005804 alkylation reaction Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000588881 Chromobacterium Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000159512 Geotrichum Species 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002152 alkylating effect Effects 0.000 description 1
- 210000001557 animal structure Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 125000002704 decyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 239000012052 hydrophilic carrier Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 235000019626 lipase activity Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 238000005887 phenylation reaction Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 108010072641 thermostable lipase Proteins 0.000 description 1
- 125000002221 trityl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C([*])(C1=C(C(=C(C(=C1[H])[H])[H])[H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- 238000005866 tritylation reaction Methods 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57029252A JPS58146284A (ja) | 1982-02-25 | 1982-02-25 | リパ−ゼによる油脂の加水分解法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57029252A JPS58146284A (ja) | 1982-02-25 | 1982-02-25 | リパ−ゼによる油脂の加水分解法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58146284A JPS58146284A (ja) | 1983-08-31 |
| JPH049516B2 true JPH049516B2 (enrdf_load_stackoverflow) | 1992-02-20 |
Family
ID=12271074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57029252A Granted JPS58146284A (ja) | 1982-02-25 | 1982-02-25 | リパ−ゼによる油脂の加水分解法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58146284A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017001036A (ja) * | 2010-12-29 | 2017-01-05 | 天野エンザイム株式会社 | 含油脂廃水処理における、微生物への負荷、余剰微生物生成量及び消費電力量の低減方法 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4131546A1 (de) * | 1991-09-21 | 1993-03-25 | Chemie Linz Deutschland | Verfahren zur erhoehung der enantioselektivitaet einer candida lipase bei der veresterung chiraler alkohole und eine immobilisierte candida lipase |
| US6258575B1 (en) | 1998-11-26 | 2001-07-10 | Kao Corporation | Hydrolyzing fats and oils using an immobilized enzyme column and substrate-feeding chamber that separates phases |
-
1982
- 1982-02-25 JP JP57029252A patent/JPS58146284A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017001036A (ja) * | 2010-12-29 | 2017-01-05 | 天野エンザイム株式会社 | 含油脂廃水処理における、微生物への負荷、余剰微生物生成量及び消費電力量の低減方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS58146284A (ja) | 1983-08-31 |
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