JPH0422553B2 - - Google Patents

Info

Publication number
JPH0422553B2
JPH0422553B2 JP63224842A JP22484288A JPH0422553B2 JP H0422553 B2 JPH0422553 B2 JP H0422553B2 JP 63224842 A JP63224842 A JP 63224842A JP 22484288 A JP22484288 A JP 22484288A JP H0422553 B2 JPH0422553 B2 JP H0422553B2
Authority
JP
Japan
Prior art keywords
mmol
restriction endonuclease
cells
nnn
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63224842A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0198484A (ja
Inventor
Jon Boruton Buraian
Yarushu Mihyaeru
Shumitsutsu Guutorun
Kesuraa Kurisutofu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of JPH0198484A publication Critical patent/JPH0198484A/ja
Publication of JPH0422553B2 publication Critical patent/JPH0422553B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
JP63224842A 1987-09-09 1988-09-09 ２型−制限エンドヌクレアーゼ、その取得法、相補的二本鎖ｄｎａ−配列を認識及び切断する方法 Granted JPH0198484A (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3730247 1987-09-09
DE3730247.7 1987-09-09
DE3826389A DE3826389A1 (de) 1987-09-09 1988-08-03 Typ ii-restriktionsendonuklease ksp632i
DE3826389.0 1988-08-03

Publications (2)

Publication Number Publication Date
JPH0198484A JPH0198484A (ja) 1989-04-17
JPH0422553B2 true JPH0422553B2 (en:Method) 1992-04-17

Family

ID=25859599

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63224842A Granted JPH0198484A (ja) 1987-09-09 1988-09-09 ２型−制限エンドヌクレアーゼ、その取得法、相補的二本鎖ｄｎａ−配列を認識及び切断する方法

Country Status (5)

Country Link
US (1) US4970149A (en:Method)
EP (1) EP0306847B1 (en:Method)
JP (1) JPH0198484A (en:Method)
DE (2) DE3826389A1 (en:Method)
ES (1) ES2061578T3 (en:Method)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4032213A1 (de) * 1990-10-11 1992-04-16 Boehringer Mannheim Gmbh Typ ii-restriktionsendonuklease ssp4800i
DE19641952C5 (de) * 1996-10-11 2005-06-02 Daimlerchrysler Ag Kraftstofführung für eine mehrzylindrige Brennkraftmaschine mit Aufnahmebohrungen für Steckpumpen
JP3467185B2 (ja) 1998-04-08 2003-11-17 矢崎総業株式会社 コネクタのロック機構

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3512435A1 (de) * 1984-06-13 1986-01-02 Boehringer Mannheim Gmbh, 6800 Mannheim Restriktionsendonuklease asp 718, ihre gewinnung und verwendung

Also Published As

Publication number Publication date
US4970149A (en) 1990-11-13
EP0306847B1 (de) 1993-12-15
EP0306847A2 (de) 1989-03-15
EP0306847A3 (en) 1989-05-17
DE3826389A1 (de) 1989-03-30
JPH0198484A (ja) 1989-04-17
ES2061578T3 (es) 1994-12-16
DE3886310D1 (de) 1994-01-27

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