JPH03193711A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic having spread and a dampish feeling and improving texture and gloss of skin by combining a conchiolin hydrolysate and/or a hydrolysate of pearl oyster mucus with a polysaccharide obtained from cultivation of aureobasidium pullulan. CONSTITUTION:A hydrolysate of conchiolin obtained by finely crushing insoluble component obtained from shellfishes such as pearl oyster or hard-shelled mussel with ash-removing treatment of acid and/or a hydrolysate of pearl oyster mucus obtained from pearl oyster with hydrolyzing of mucus is mixed with a polysaccharide (a natural neutral mucopolysaccharide mainly having regular alpha-1,6 bonding of maltotriose) obtained from aureobasidium pullulan with cultivation and contained in the aimed cosmetic. Said cosmetic has excellent spread and a dampish feeling with making skin to have more finer texture and better gloss and also has excellent safety and stability.

Description

[Detailed Description of the Invention] [Industrial Application Field] A cosmetic that spreads on the cosmetic, imparts a moist feeling, and improves the texture and luster of the skin, and has excellent safety and stability. Regarding.

[Conventional technology]

Regarding conchiolin hydrolyzate and pearl oyster mucus hydrolyzate, JP-A-62-221612 and JP-A-62
It has been shown in Japanese Patent Application Laid-open No. 223104 and Japanese Patent Application Laid-Open No. 63-57507 that it is a substance worthy of special mention as a raw material for cosmetics.

Conchiolin is made by decalcifying shellfish such as pearl oysters, mussels, and mussels with acid, collecting insoluble matter, and finely pulverizing this. % hydrochloric acid aqueous solution, and then remove the hydrochloric acid to obtain a hydrolyzate of conchiolin (this is also simply called conchiolin).
.

The pearl oyster mucus hydrolyzate also has the same components as the conchiolin hydrolyzate as its main component.

The polysaccharide obtained by culturing Aureobasidum pullulan is a natural neutral viscous polysaccharide consisting mainly of malitotriose (3 molecules of glucose linked together with α-1,4 bonds) with regular α-1,6 bonds. It is.

This polysaccharide (also called pullulan) is widely used in the food field. It is used as an excipient, a quality improver, a film forming agent, and a stabilizer by taking advantage of its properties of adhesion, consolidation, tackiness, and film properties.
It is also used in some cosmetics.

There are various functions required of cosmetics, but they can be broadly divided into the following four points. 1. Safety, 2. Stability, 3. Sensual aspect, 4. It is effectiveness.

Although various studies have been conducted on these issues in the past, they have not yet been resolved.

[Problem to be solved by the invention]

An object of the present invention is to provide a cosmetic that spreads on the cosmetic, gives a moisturizing feeling, improves the texture and luster of the skin, and is highly safe and has excellent stability. .

[Means to solve the problem]

As a result of intensive research into the development of cosmetics with improved effectiveness, safety, stability, and sensory aspects and new effectiveness, the present inventors discovered that conchiolin hydrolyzate and/or pearl oyster mucus hydrolyzate Things and Aureobasidium pullulan (Au
An unprecedented cosmetic was obtained by incorporating it into a cosmetic in combination with a polysaccharide obtained by culturing S. reobasidlum pullulans.

That is, the present invention provides conchiolin hydrolyzate and/or pearl oyster mucus hydrolyzate, and Aureobasidlum pullulans.
) is a cosmetic containing a polysaccharide obtained by culturing.

Regarding conchiolin hydrolyzate and pearl oyster mucus hydrolyzate, JP-A-62-221612 and JP-A-62
The manufacturing method and content thereof are described in detail in JP-A-223104, JP-A-63-57507, etc., and the necessary raw materials are obtained according to the manufacturing method.

To briefly summarize, conchiolin is obtained by decalcifying shellfish such as pearl oysters, mussels, and mussels with acid, collecting insoluble matter, and finely pulverizing this. 0~ using 15% hydrochloric acid aqueous solution
Hydrolysis is carried out by treatment for 30 minutes to 10 days while controlling the temperature at 130°C. Hydrochloric acid is removed from this hydrolyzate to obtain a hydrolyzate of conchiolin (sometimes this is simply called conchiolin).

A pearl oyster mucus hydrolyzate is obtained by removing the shell of a pearl oyster and collecting the mucus by decantation, filtration, centrifugation, etc. Usually, this slime is heated to about 120 to 150° C. under pressure to carry out precipitation treatment.

This precipitate is hydrolyzed with acid, alkali, or enzyme to obtain a mucus hydrolyzate.

It is preferable to use a proteolytic enzyme such as trypsin, papain, pronase, etc., because the specificity of the enzyme can be used to obtain a peptide of the required molecular weight, there is no decomposition of tryptophan, etc., and there are few by-products. When used in cosmetics, especially in emulsions, the emulsification may be broken, so further desalting is preferred.

On the other hand, Aureobacidium pullulan (Aureob
The polysaccharide obtained by culturing P. acidlum pullulans is a natural neutral viscous polysaccharide (also called pullulan) in which malitotriose (3 glucose molecules are linked with α-1,4 bonds) is regularly linked with α-1,6 bonds. It is.

Although conchiolin and pullulan have been used separately in cosmetics, there are no known cosmetics that use the two in combination. When these two were combined and blended into cosmetics, a large synergistic effect was found, rather than the sum of their respective effects. This is the present invention.

Conchiolin hydrolyzate and/or pearl oyster mucus hydrolyzate is 0.01% to 10% by weight, and polysaccharide obtained by culturing Aureobasidum pullulan is 0.1% to 10% by weight. It was found that the intended purpose could be achieved by doing so.

Other raw materials are not particularly limited, but liquid oils include liquid paraffin, squalane, olive oil, jojoba oil, safflower oil, avocado oil, mink oil, myristyl myristate, isopropyl myristate, octyldodecyl myristate, and palmitic acid. 2-ethylhexyl,
Cetyl 2-ethyl hexanoate, 2-ethylhexanoic acid triglyceride, caprylic caproic acid triglyceride, methylphenyl polysiloxane, dimethyl polysiloxane, Solid oils include paraffin, ceresin, microcrystalline wax, beeswax, gay wax, beef tallow, and laurin. Acid, myristic acid, valmitic acid, stearic acid, behenic acid, oleic acid, isostearic acid, cetyl alcohol, stearyl alcohol, behenyl alcohol, moisturizers include glycerin, propylene glycol, dipropylene glycol, 1,3 butylene glycol, P
EG100O, sorbitol, mannitol; Thickeners such as carboxymethylcellulose, polyvinyl alcohol, carboxyvinyl polymer polyvinylpyrrolidone; Nonionic, anionic, and amphoteric surfactants can be used.

In addition, ethyl alcohol, vitamins, preservatives, fragrances, natural extracts, antioxidants, etc. can be used, but the use of unsafe raw materials that go against the spirit of the present invention should of course be avoided. It is.

〔Example〕

The present invention will be explained in more detail below with reference to Examples, but the present invention is not limited to these Examples in any way. The units used in the examples are as follows, unless otherwise specified:
Weight%.

(Example-1) A Liquid paraffin 24
．． 5 Beeswax 5
．． 0 jojoba oil 7
．． 0 stearyl alcohol 2
．． 0 Stearic acid monoglyceride 2
．． 0 sorbitan monostearate
3.5 polyoxyethylene sorbitan
4.0 Monostearate (60E, O,)B Purified Water 45.41.
3 Butylene glycol 5.0 Polysaccharide obtained by culturing Aureobasiduum pullulan 0.5 Methyl roseoxybenzoate 0.2
C fragrance 0.2 Conchiolin hydrolyzate 0.2
Weigh A and B, respectively, and dissolve them by heating at 80°C. While stirring B, gradually add A and at about 60°C, add C and cool to room temperature.

(Example-2) Emulsion A Liquid paraffin 5
．． 0 Octyldodecyl myristate 2
．． 0-tri-2-ethylhexanoic acid glyceride 2
．． 5 stearyl alcohol 1
．． 5 cetyl alcohol 0
．． 5 Monostearic acid glyceride 1
．． 5 stearic acid
1,5 myristic acid
1.5 Sorbitan Monostearate 1. OB Purified water 71
．． 91.3 Butylene glycol 5
．． 0 glycerin 2.0
NaOH0,2 Aureobasidium pullulan cultured 3.0
Obtained polysaccharide rose methyl oxybenzoate 0.2
C fragrance 0.2 Akoya mucus hydrolyzate 0.5
Weigh A and B, respectively, and dissolve them by heating at 80°C. While stirring B, gradually add A and at about 60°C, add C and cool to room temperature.

(Comparative example) Efficacy was compared based on raw materials.

The safety test was compared with other companies' products.

As a comparison of Example 1, Company A's Rireme (A-1) and Company 8 Cream (B-1); as a comparison of Example 2, Company A's emulsion (A-2) and Company C's emulsion (C-1). was used.

Sensory properties and stability were tested as follows by replacing some of the raw materials.

Comparative Example 1-1 is a sample of Aureobasidium* pullulan from Example-1.
Comparative Example 1-2 is from Example 1, except for the polysaccharide obtained by culturing A. lans). Comparative Example 1-3 is from Example 1, except for the conchiolin hydrolyzate. Aureobasidium pullu
Comparative Example 2-1 is a sample obtained by culturing Aureobasidum pullulan and Conchiolin hydrolyzate, excluding the polysaccharide obtained from Example-2.
Comparative Example 2-2 is based on Example 2, except for the polysaccharide obtained by culturing A. Aureobasidium pullu
[Safety test] Method 1) Cytotoxicity test ■) Eag in a 6CI+ chalet with a cover glass
5 m of le MEM (20% beef tallow serum) medium
Dispense in liter portions.

2) Add JTC-17 cell suspension (300,000 cells) to 5%
Co, 36°C, culture for 48 hours.

3) Buffer PBS (-) (NaC 18g.

KCI O, 2g, Na PO1, 15g.

4 Wash twice with buffer containing 2 g of KHPOO 4) Add 3 ml of sample diluted with buffer PBS (-) and incubate at 36°C for 60 minutes.

5) Eagle MEM (20% beef tallow serum)
Dispense the medium into 7ml portions and culture at 5% CO, 36°C for 48 hours.

6) Fix, stain and judge.

Judgment criteria [1] Judgment based on cell number 5 cores O: Same 5 cores as Control
1: Almost the same as Control (slightly inferior staining density) Score 2: 2/3 of Control (decreased enough to be visible to the naked eye) Score 3: Almost half of Control (significantly decreased) Score 4: 1/1 of Control 3rd place (very few cells) Score 5: No cells observed (no or almost no cells observed) [2] Judgment based on cell morphology 5core O: Normal image 5core 1: Slight abnormal cells observed (normal cells 80% or more) Score 2: Abnormal cells are observed (normal cells are about 50% to 80%) Score 3: Many abnormal cells are observed (normal cells are about 20% to 50%) Score 4: Most of the abnormal cells are observed be.

(Normal cells are approximately 10%) Score 5: Normal cells are not recognized. The first number indicates the determination result based on cell number, and the number in parentheses indicates the determination result based on cell morphology.

2) Human closed batch test 1) Apply the specimen to the plaster (fin chamber),
Apply it on the inside of your upper arm and leave it for 24 hours.

2) Peel off the plaster and leave it for 1 hour.

(Judgment method) Judgment is made visually using a fluorescent light with a magnifying glass.

(Judgment criteria) Judgment irritation point 0.00 No reaction 1 o, oo Suspicious erythema, slight erythema ± 0
．． 50 Mild erythema + 1.00 Erythema 44 2.00 Erythema + edema + + + 3.00 Erythema + edema + papules, serous papules, vesicles Irritation rate - total irritation points / total number of people x 1003) Rabbit closed batch test Japanese white Six seed rabbits are used. Separate the back skin with electric clippers and apply 0.2 of the sample to the veterinary batch test plaster.
Add 1ml drop and apply it to the skin for 24 hours.

Judgment is made based on the following criteria at 1.24.48.72 hours after removal of the patch.

(Judgment criteria) Erythema 5 cores 0 3 cores 1 No erythema Very mild erythema (barely noticeable) Obvious erythema Moderate to strong erythema Strong crimson erythema with mild crust formation (disorder is deep) Score 2 Score 3 Score Edema Score Score Score Score No edema Very mild edema (barely noticeable) Obvious edema (clearly distinguishable from the surrounding area) Moderate edema (raised by about 1■■) Score 4 Strong edema (raised by 11 or more per ml) 4) Guinea pig continuous application test ■) Using 6 Hartley white guinea pigs, the skin on the back and flanks was removed with electric clippers and a shaver, and the skin was applied within an area of 2a11 in diameter using a cotton swab.

2) Apply this application once a day for 3 consecutive days.

(Judgment) (Judgment criteria) Erythema and edema are measured, but it is the same as the rabbit closed batch test.

The cumulative stimulation index was 72tlr, 96h, and 168h.
This is the average value of the latter 5 cores.

[Results]

1) Cytotoxicity test 3) Rabbit closed batch test 2) Human closed batch test 4) Guinea pig continuous application test [Efficacy test] Cell activation test
Add 5 ml of IoMEM (20% fetal bovine serum) medium.
Dispense in liter portions.

2) Add JTC-17 cell suspension (200,000 cells) to 5%
Incubate at CO, 36°C for 48 hours.

3) Buffer PBS (-) (NaC 18g.

KCI 0.2g, Na PO1.15g.

4 Wash twice with a buffer solution containing 0.2 g of KHPO) 4) Add 5 ml of the sample diluted to each concentration with Eagle MEM (serum-free) medium and 0.05 ml of fetal bovine serum, and incubate in 5% Co, 36°C. , and cultured for 6 days.

5) Fix, stain and judge.

Judgment criteria [1] Judgment based on cell number 5core -3: Approximately 1.5 times of Control (
Score -2: 4/3 of Control (increase to the extent that it can be seen with the naked eye) Score -1: Same as Control (slightly better staining density) Score O: Same 5 cores as Control
1: Almost the same as Control (slightly inferior staining density) Score 2: 2/3 of Control (decreased to the extent that it can be seen with the naked eye) [2] Judgment based on cell morphology 5 cores O: Normal image 5 cores 1: Few abnormal cells Score 2: Abnormal cells are observed (normal cells are around 50% to 80%) Score 3: Many abnormal cells are observed (normal cells are around 20% to 50%) Score 4: Almost all cells are abnormal.

(Normal cells are around 10%) Score 5: Normal cells are not recognized The first number indicates the determination result based on the number of cells, and the number in parentheses indicates the determination result based on cell morphology. The blank was 0(3).

[Sensory test and stability test]

In the sensory test, 96 women were divided into 8 groups, and each group was randomly tested using 4 samples for 1 month each.

(48 people per sample) The evaluation was done on a 5-level scale.

Rating 5 represents the following condition.

〔Effect of the invention〕

In all of the above-mentioned efficacy tests, safety tests, sensory tests, and stability tests, conchiolin hydrolysates and/or pearl oyster mucus hydrolysates were obtained by culturing Aureobasidum pullulan. It is clear that cosmetics containing both polysaccharides and polysaccharides are significantly superior in efficacy, safety, sensation, and stability compared to when they are blended separately. This is due to the combined effect of containing both of them, and as a result, it was possible to obtain a cosmetic that had not been previously available.

Claims (1)

[Claims] 1. Conchiolin hydrolyzate and/or pearl oyster mucus hydrolyzate and Aureobasidum pullulan (Aureo
A cosmetic comprising a polysaccharide obtained by culturing basidium pullulans.