JPH03101601A - 植物胚芽の保存方法 - Google Patents
植物胚芽の保存方法Info
- Publication number
- JPH03101601A JPH03101601A JP2189262A JP18926290A JPH03101601A JP H03101601 A JPH03101601 A JP H03101601A JP 2189262 A JP2189262 A JP 2189262A JP 18926290 A JP18926290 A JP 18926290A JP H03101601 A JPH03101601 A JP H03101601A
- Authority
- JP
- Japan
- Prior art keywords
- embryos
- oil
- embryo
- germ
- stored
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 23
- 235000013311 vegetables Nutrition 0.000 title claims 2
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 38
- 206010021143 Hypoxia Diseases 0.000 claims abstract description 29
- 239000002775 capsule Substances 0.000 claims abstract description 5
- 239000011248 coating agent Substances 0.000 claims abstract description 5
- 238000000576 coating method Methods 0.000 claims abstract description 5
- 229940057995 liquid paraffin Drugs 0.000 claims abstract description 5
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 3
- 230000001146 hypoxic effect Effects 0.000 claims abstract 3
- 239000003921 oil Substances 0.000 claims description 38
- 230000007954 hypoxia Effects 0.000 claims description 25
- 241000196324 Embryophyta Species 0.000 claims description 14
- 244000052616 bacterial pathogen Species 0.000 claims description 14
- 230000000392 somatic effect Effects 0.000 claims description 9
- 240000007154 Coffea arabica Species 0.000 claims description 7
- 244000000626 Daucus carota Species 0.000 claims description 6
- 235000002767 Daucus carota Nutrition 0.000 claims description 6
- 230000035601 cold sensitivity Effects 0.000 claims description 5
- 235000007460 Coffea arabica Nutrition 0.000 claims description 4
- 239000005662 Paraffin oil Substances 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 3
- 239000002480 mineral oil Substances 0.000 claims description 2
- 235000010446 mineral oil Nutrition 0.000 claims description 2
- 210000002257 embryonic structure Anatomy 0.000 abstract description 72
- 210000004027 cell Anatomy 0.000 abstract description 10
- 239000000725 suspension Substances 0.000 abstract description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 5
- 239000001301 oxygen Substances 0.000 abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 abstract description 5
- 238000001816 cooling Methods 0.000 abstract description 4
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 239000003755 preservative agent Substances 0.000 abstract description 2
- 230000002335 preservative effect Effects 0.000 abstract description 2
- 238000002224 dissection Methods 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 description 31
- 239000002609 medium Substances 0.000 description 24
- 230000035784 germination Effects 0.000 description 20
- 238000003860 storage Methods 0.000 description 17
- 239000007788 liquid Substances 0.000 description 12
- 238000009630 liquid culture Methods 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000000284 resting effect Effects 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- LCPDWSOZIOUXRV-UHFFFAOYSA-N phenoxyacetic acid Chemical compound OC(=O)COC1=CC=CC=C1 LCPDWSOZIOUXRV-UHFFFAOYSA-N 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 208000013016 Hypoglycemia Diseases 0.000 description 1
- 240000001140 Mimosa pudica Species 0.000 description 1
- 235000016462 Mimosa pudica Nutrition 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 239000007798 antifreeze agent Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 230000003416 augmentation Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000004368 gonadotroph Anatomy 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007103 stamina Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8909639 | 1989-07-18 | ||
| FR8909639A FR2649860A1 (fr) | 1989-07-18 | 1989-07-18 | Procede de conservation d'embryons vegetaux |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03101601A true JPH03101601A (ja) | 1991-04-26 |
Family
ID=9383871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2189262A Pending JPH03101601A (ja) | 1989-07-18 | 1990-07-17 | 植物胚芽の保存方法 |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5138793A (OSRAM) |
| EP (1) | EP0408922B1 (OSRAM) |
| JP (1) | JPH03101601A (OSRAM) |
| AP (1) | AP158A (OSRAM) |
| AT (1) | ATE113072T1 (OSRAM) |
| AU (1) | AU629369B2 (OSRAM) |
| BR (1) | BR9003442A (OSRAM) |
| CA (1) | CA2020572C (OSRAM) |
| DE (1) | DE69013423T2 (OSRAM) |
| DK (1) | DK0408922T3 (OSRAM) |
| ES (1) | ES2064534T3 (OSRAM) |
| FI (1) | FI96375C (OSRAM) |
| FR (1) | FR2649860A1 (OSRAM) |
| IE (1) | IE65060B1 (OSRAM) |
| MX (1) | MX171281B (OSRAM) |
| MY (1) | MY105818A (OSRAM) |
| NO (1) | NO179352C (OSRAM) |
| NZ (1) | NZ234524A (OSRAM) |
| OA (1) | OA09218A (OSRAM) |
| PT (1) | PT94720B (OSRAM) |
| ZA (1) | ZA905204B (OSRAM) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100814625B1 (ko) * | 2001-06-28 | 2008-03-18 | 모리시타 진탄 가부시키가이샤 | 살아 있는 세포 또는 조직을 포함하는 캡슐 |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ246137A (en) | 1991-12-19 | 1996-04-26 | Univ Saskatchewan | Mature desiccation tolerant gymnosperm somatic embryos and their production |
| US6340594B1 (en) | 1991-12-19 | 2002-01-22 | Cellfor, Inc. | Production of desiccation-tolerant gymnosperm embryos |
| NZ272210A (en) * | 1995-05-25 | 1997-10-24 | Carter Holt Harvey Ltd | Treatment of somatic embryos to provide viable embryos after storage periods |
| EP0816488A1 (fr) * | 1996-06-25 | 1998-01-07 | Societe Des Produits Nestle S.A. | Procédé de conditionnement de tissus de végétaux cultivés in-vitro |
| JP4476442B2 (ja) * | 2000-06-20 | 2010-06-09 | アグリテクノ矢崎株式会社 | トルコギキョウのロゼット化による発育不良防止方法 |
| WO2002101018A2 (en) * | 2001-06-11 | 2002-12-19 | Fred Hutchinson Cancer Research Center | Methods for inducing reversible stasis |
| US20120131849A1 (en) * | 2009-06-25 | 2012-05-31 | Masanori Asada | Method for storage of seeds |
| WO2013003288A1 (en) * | 2011-06-29 | 2013-01-03 | Weyerhaeuser Nr Company | Manufactured seed having embryo disposed therein |
| US20150191771A1 (en) * | 2014-01-09 | 2015-07-09 | Syngenta Participations Ag | Embryo sampling method |
| US9078427B1 (en) * | 2014-08-29 | 2015-07-14 | Pioneer Hi Bred International Inc | Method of storing plant embryos |
| BR112017003738A2 (pt) | 2014-08-29 | 2017-12-26 | Pioneer Hi Bred Int | ?métodos de armazenamento de dna genômico, de armazenamento de materiais de ensaio de marcador molecular, de tratamento de um ou mais embriões vegetais, para seleção de um ou mais embriões vegetais e de transformação de tecido vegetal? |
| US11111548B2 (en) * | 2014-08-29 | 2021-09-07 | Pioneer Hi-Bred International, Inc. | Systems and methods for genotyping seed components |
| US9706723B2 (en) * | 2014-08-29 | 2017-07-18 | Pioneer Hi-Bred International, Inc. | Methods for creating doubled haploid maize embryos using oil matrices |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60105601A (ja) * | 1983-08-26 | 1985-06-11 | フエリツクス・フランクス | 低温保存法 |
| JPS60214811A (ja) * | 1983-10-25 | 1985-10-28 | プラント・ジエネテイクス・インコ−ポレ−テツド | 分裂組織を植付ける方法 |
| JPS6140708A (ja) * | 1984-07-31 | 1986-02-27 | ライオン株式会社 | 人工種子 |
| JPS62138107A (ja) * | 1985-12-10 | 1987-06-20 | フロイント産業株式会社 | 組成物およびその製造方法 |
| JPS62257320A (ja) * | 1986-04-28 | 1987-11-09 | 株式会社 日本グリンナ− | 穂発芽の予防、抑制法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2091534B (en) * | 1980-12-09 | 1984-06-27 | Boc Lit | Method of preservation |
| US4397681A (en) * | 1981-06-08 | 1983-08-09 | Fats And Proteins Research Foundation, Inc. | Foliar antitranspirant |
| US4615141A (en) * | 1984-08-14 | 1986-10-07 | Purdue Research Foundation | Process for encapsulating asexual plant embryos |
| WO1989005575A1 (en) * | 1987-12-18 | 1989-06-29 | University Of Florida | Quiescent plant somatic embryos and method for their production |
| DE3882528T2 (de) * | 1988-09-19 | 1993-11-11 | Nestle Sa | Verfahren zur Produktion von PflanzensubstanzEN. |
-
1989
- 1989-07-18 FR FR8909639A patent/FR2649860A1/fr active Granted
-
1990
- 1990-06-22 AT AT90111821T patent/ATE113072T1/de active
- 1990-06-22 DE DE69013423T patent/DE69013423T2/de not_active Expired - Fee Related
- 1990-06-22 DK DK90111821.6T patent/DK0408922T3/da active
- 1990-06-22 EP EP90111821A patent/EP0408922B1/fr not_active Expired - Lifetime
- 1990-06-22 ES ES90111821T patent/ES2064534T3/es not_active Expired - Lifetime
- 1990-06-25 US US07/543,475 patent/US5138793A/en not_active Expired - Lifetime
- 1990-06-27 FI FI903229A patent/FI96375C/fi not_active IP Right Cessation
- 1990-06-27 IE IE232590A patent/IE65060B1/en not_active IP Right Cessation
- 1990-07-02 AU AU58674/90A patent/AU629369B2/en not_active Ceased
- 1990-07-03 ZA ZA905204A patent/ZA905204B/xx unknown
- 1990-07-06 CA CA002020572A patent/CA2020572C/en not_active Expired - Fee Related
- 1990-07-09 MX MX021506A patent/MX171281B/es unknown
- 1990-07-13 NO NO903138A patent/NO179352C/no not_active IP Right Cessation
- 1990-07-16 MY MYPI90001188A patent/MY105818A/en unknown
- 1990-07-16 AP APAP/P/1990/000193A patent/AP158A/en active
- 1990-07-16 NZ NZ234524A patent/NZ234524A/xx unknown
- 1990-07-17 PT PT94720A patent/PT94720B/pt not_active IP Right Cessation
- 1990-07-17 JP JP2189262A patent/JPH03101601A/ja active Pending
- 1990-07-17 BR BR909003442A patent/BR9003442A/pt not_active IP Right Cessation
- 1990-07-18 OA OA59819A patent/OA09218A/xx unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60105601A (ja) * | 1983-08-26 | 1985-06-11 | フエリツクス・フランクス | 低温保存法 |
| JPS60214811A (ja) * | 1983-10-25 | 1985-10-28 | プラント・ジエネテイクス・インコ−ポレ−テツド | 分裂組織を植付ける方法 |
| JPS6140708A (ja) * | 1984-07-31 | 1986-02-27 | ライオン株式会社 | 人工種子 |
| JPS62138107A (ja) * | 1985-12-10 | 1987-06-20 | フロイント産業株式会社 | 組成物およびその製造方法 |
| JPS62257320A (ja) * | 1986-04-28 | 1987-11-09 | 株式会社 日本グリンナ− | 穂発芽の予防、抑制法 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100814625B1 (ko) * | 2001-06-28 | 2008-03-18 | 모리시타 진탄 가부시키가이샤 | 살아 있는 세포 또는 조직을 포함하는 캡슐 |
Also Published As
| Publication number | Publication date |
|---|---|
| NO179352C (no) | 1996-09-25 |
| PT94720B (pt) | 1997-07-31 |
| BR9003442A (pt) | 1991-08-27 |
| IE65060B1 (en) | 1995-10-04 |
| NO179352B (no) | 1996-06-17 |
| CA2020572A1 (en) | 1991-01-19 |
| AU5867490A (en) | 1991-01-24 |
| MX171281B (es) | 1993-10-15 |
| IE902325A1 (en) | 1991-06-19 |
| EP0408922A1 (fr) | 1991-01-23 |
| ES2064534T3 (es) | 1995-02-01 |
| NO903138D0 (no) | 1990-07-13 |
| OA09218A (en) | 1992-06-30 |
| AP9000193A0 (en) | 1990-07-31 |
| MY105818A (en) | 1995-01-30 |
| PT94720A (pt) | 1991-03-20 |
| DE69013423T2 (de) | 1995-02-23 |
| NO903138L (no) | 1991-01-21 |
| US5138793A (en) | 1992-08-18 |
| NZ234524A (en) | 1991-10-25 |
| CA2020572C (en) | 2000-03-28 |
| FR2649860A1 (fr) | 1991-01-25 |
| DK0408922T3 (da) | 1995-02-27 |
| FR2649860B1 (OSRAM) | 1994-08-19 |
| FI96375B (fi) | 1996-03-15 |
| ATE113072T1 (de) | 1994-11-15 |
| AU629369B2 (en) | 1992-10-01 |
| EP0408922B1 (fr) | 1994-10-19 |
| ZA905204B (en) | 1991-04-24 |
| DE69013423D1 (de) | 1994-11-24 |
| FI96375C (fi) | 1996-06-25 |
| AP158A (en) | 1991-11-15 |
| FI903229A0 (fi) | 1990-06-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chang et al. | A tissue culture protocol for propagation of a rare plant, Lilium speciosum Thunb. var. gloriosoides Baker | |
| JPH03101601A (ja) | 植物胚芽の保存方法 | |
| CA2700337A1 (en) | Plant stem cell line derived from quiescent center and method for isolating the same | |
| Grout et al. | Practical aspects of the propagation of cauliflower by meristem culture | |
| Chapman | Potato tissue cultures | |
| Gale et al. | Developing cryopreservation for Picea sitchensis (Sitka spruce) somatic embryos: a comparison of vitrification protocols | |
| CN106234222B (zh) | 一种山桐子叶片组织培养方法 | |
| US7229828B2 (en) | Sugar cane production | |
| CN109042330A (zh) | 一种桃叶卫矛的组织培养方法 | |
| Haynes | Potato embryo culture | |
| JPH0220226A (ja) | ナガイモの不定胚培養法 | |
| WO1996013577A1 (en) | Colored plant culture media | |
| Vishnevetsky et al. | Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro | |
| JPS6371121A (ja) | 組織培養によるナガイモムカゴの大量生産法 | |
| Taha et al. | Production of synthetic seeds from micro shoots and somatic embryos of Gerbera jamesonii bolus ex. hook f. | |
| Dias et al. | Somatic embryogenesis and plant regeneration in the tissue culture of Geonoma gamiova (Arecaceae) | |
| MXPA03003111A (es) | Metodos para producir rendimientos altos de embriones de pino cotiledonarios de apariencia cigotica utilizando medios que incluyen un disacarido y glucosa. | |
| Sereda et al. | Use of rocker bioreactor for propagation of the Vaccinium corymbosum L. | |
| JP3080784B2 (ja) | フウロソウの大量増殖方法 | |
| Grout | Cryopreservation of excised meristems, shoot tips and somatic embryos | |
| JP2908954B2 (ja) | スミレ属植物の容器内開花による生産方法 | |
| Hiraoka | Nonfrozen storage of plant cell cultures and its effect on metabolites | |
| Maldaner et al. | Nutrient concentrations of in vitro culture is crucial for Desmodium incanum DC. Acclimatization | |
| Nicolae et al. | Research on the use of synthetic seed in conservation of Kalanchoe blossfeldiana. | |
| JPH02312530A (ja) | ギョウジャニンニクの大量増殖法 |