JPH0283329A - Production of chloropyll preparation - Google Patents
Production of chloropyll preparationInfo
- Publication number
- JPH0283329A JPH0283329A JP63236981A JP23698188A JPH0283329A JP H0283329 A JPH0283329 A JP H0283329A JP 63236981 A JP63236981 A JP 63236981A JP 23698188 A JP23698188 A JP 23698188A JP H0283329 A JPH0283329 A JP H0283329A
- Authority
- JP
- Japan
- Prior art keywords
- chlorophyll
- protein
- containing substance
- solution
- ethanol solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 238000004519 manufacturing process Methods 0.000 title claims description 10
- 229930002875 chlorophyll Natural products 0.000 claims abstract description 43
- 235000019804 chlorophyll Nutrition 0.000 claims abstract description 43
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 42
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims abstract description 42
- 239000000243 solution Substances 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 14
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 14
- 239000000126 substance Substances 0.000 claims abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 8
- 238000004898 kneading Methods 0.000 claims abstract description 3
- 238000002156 mixing Methods 0.000 claims abstract description 3
- 239000000463 material Substances 0.000 claims description 7
- 238000002604 ultrasonography Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 abstract description 9
- 238000003756 stirring Methods 0.000 abstract description 8
- 210000003763 chloroplast Anatomy 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 241000255789 Bombyx mori Species 0.000 abstract description 3
- 101710120037 Toxin CcdB Proteins 0.000 abstract description 3
- 210000003608 fece Anatomy 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 230000002079 cooperative effect Effects 0.000 abstract description 2
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 210000000170 cell membrane Anatomy 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 241000195628 Chlorophyta Species 0.000 description 1
- 241000199919 Phaeophyceae Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 239000001752 chlorophylls and chlorophyllins Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 229940012356 eye drops Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 206010040872 skin infection Diseases 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/58—Metal complex; Coordination compounds
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Seeds, Soups, And Other Foods (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
- Confectionery (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、健康食品、化粧品等に使用した場合、吸収の
よい葉緑素製剤の製法に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for producing a chlorophyll preparation that is well absorbed when used in health foods, cosmetics, and the like.
従来、葉緑素には殺菌作用があり、皮、２戻患、潰瘍、
火傷などの回復促進作用、悪臭の防止などがあるため、
歯あ粉、チューインガム、キマラメルなどの口腔清浄剤
、食品類又はポマード、石鹸などの化粧料、目薬などの
医薬品などに添加して広く用いられている。Traditionally, chlorophyll has a bactericidal effect and is effective against skin infections, ulcers,
Because it promotes recovery from burns and prevents bad odors,
It is widely used as an additive to toothpaste, chewing gum, oral cleansers such as Chimalamel, foods, cosmetics such as pomades and soaps, and pharmaceuticals such as eye drops.
しかしながら、これらの葉緑素は葉緑体を含有する緑色
植物、藻類等からエタノールで抽出して用いられていた
。しかるに、葉緑素は葉緑禦含を物中に細胞膜を介して
存在しているので細抱内の葉緑素を抽出する場合は、こ
の細胞膜があるため十分に抽出処理ができず、また人体
が摂取した場合、吸収性が悪いという問題があった。However, these chlorophylls have been extracted with ethanol from green plants, algae, etc. containing chloroplasts. However, since chlorophyll exists in substances through cell membranes, when extracting the chlorophyll in the cells, due to the presence of cell membranes, it is not possible to extract the chlorophyll sufficiently; However, there was a problem with poor absorbency.
そこで本発明者らは葉緑素の完全な抽出と、葉緑素を主
体とする葉緑素の効果が十分に発揮される葉緑素製剤に
ついて研究を重ねたところ、葉緑素含有物をエタノール
中で超音波を照射することにより抽出し、この抽出液に
蛋白質を練合することにより、化粧料、食品などに添加
した場合、葉緑素の効果のすぐれた葉緑素製剤を見出し
本発明を完成した。Therefore, the present inventors conducted extensive research on complete extraction of chlorophyll and chlorophyll preparations that fully demonstrate the effects of chlorophyll, and found that by irradiating chlorophyll-containing substances with ultrasound in ethanol, By extracting the chlorophyll and kneading protein with this extract, they discovered a chlorophyll preparation that has excellent chlorophyll effects when added to cosmetics, foods, etc., and completed the present invention.
本発明は葉緑素含有物をエタノール溶液中で超音波によ
る処理を行い、得られた葉緑素抽出エタノール溶液と蛋
白質を練合することを特徴とする葉緑素製剤の製法であ
る。The present invention is a method for producing a chlorophyll preparation, which is characterized in that a chlorophyll-containing material is treated with ultrasound in an ethanol solution, and the obtained chlorophyll extraction ethanol solution and protein are kneaded.
本発明の原料として使用する葉緑素を含有する物は、一
般の緑葉植物、緑藻、褐藻類などの藻類、蚕の糞等の普
通の葉緑素の原料として使用されるものであれば何れも
用いることができる。The material containing chlorophyll used as a raw material in the present invention may be any material that is used as a raw material for common chlorophyll, such as general green leafy plants, algae such as green algae and brown algae, and silkworm dung. can.
本発明の方法は、上記の原料をエタノール水溶液、好適
には８０％以上の濃厚水溶液に浸漬し、この浸漬液に超
音波を照射するとともに攪拌羽根で攪拌する。この操作
により浸漬された葉緑素含有物は、葉緑体の細胞膜が破
壊され、内部の葉緑素がエタノール中に極めて良好に抽
出される。この超音波照射時間は約６０〜２００分位で
ある。In the method of the present invention, the above raw material is immersed in an ethanol aqueous solution, preferably a concentrated aqueous solution of 80% or more, and the immersion liquid is irradiated with ultrasonic waves and stirred with a stirring blade. In the chlorophyll-containing material soaked by this operation, the cell membranes of the chloroplasts are destroyed, and the chlorophyll inside is extracted extremely well into ethanol. The ultrasonic irradiation time is about 60 to 200 minutes.
このようにして得られた葉緑素抽出エタノール溶液を濾
過により葉緑素原料残渣を除去して採取する。この葉緑
素抽出エタノール液にプロティンＧ（明治製菓株式会社
製）等の蛋白質含有物好ましくは蛋白質含有量５０％以
上のものを加え練合し、これを真空濃縮機、乾燥機等を
用いて顆粒状、粉状の適宜の形状に成形して製品とする
。葉緑素液と蛋白質含有物の混合比は１：１、好適には
３：１である。The chlorophyll-extracted ethanol solution thus obtained is filtered to remove chlorophyll raw material residues and collected. A protein-containing material such as Protein G (manufactured by Meiji Seika Co., Ltd.), preferably one with a protein content of 50% or more, is added to this chlorophyll extraction ethanol solution and kneaded. , molded into an appropriate powder shape to produce a product. The mixing ratio of the chlorophyll liquid and the protein-containing material is 1:1, preferably 3:1.
第１図は、本発明による葉緑素製剤の製造装置の例を示
す概略図である。図中１は原料のエタノール水溶液を攪
拌する容器、２は循環バイブ、３は超音波攪拌槽、４は
超音波ノズル、５は超音波発振器、６は超高速回転羽根
、７は攪拌羽根、８は循環ポンプ、９は原料投入口、１
０は蓋、１１は残渣排出口である。FIG. 1 is a schematic diagram showing an example of an apparatus for producing a chlorophyll preparation according to the present invention. In the figure, 1 is a container for stirring the raw ethanol aqueous solution, 2 is a circulation vibrator, 3 is an ultrasonic stirring tank, 4 is an ultrasonic nozzle, 5 is an ultrasonic oscillator, 6 is an ultra-high-speed rotating blade, 7 is a stirring blade, 8 is a circulation pump, 9 is a raw material input port, 1
0 is a lid, and 11 is a residue outlet.
この装置を用いた具体例を以下に示す。A specific example using this device is shown below.
蚕の糞５０ｋｇを、超音波抽出装置を設置した容１１５
０ ｆｌの容器１に入れ、これに８４％エタノール水溶
液１００βを加えて攪拌した。この水溶液を循環バイブ
２により超音波攪拌槽３に送り、出力２００Ｗの超音波
ノズル４により、超音波を照射して葉緑体の細胞膜を破
壊し、葉緑素を水溶液に抽出した。これを再び容器１に
戻し、約６０００回転の超高速回転羽根６で攪拌し、６
０〜２００分間放置する。その後、容器１中の内容物を
濾過し、母液として葉緑素含有エタノール液８０ｎを得
る。得られた液は、人間の血液に似た鮮血色を呈してい
る。これを５０％以上のプロティンＧ（蛋白質７９．０
％）５０ｋｇと練合し、真空濃縮機により顆粒状に成形
する。この過程で、エタノール分は蒸散する。50kg of silkworm dung was collected in a container 115 equipped with an ultrasonic extraction device.
The mixture was placed in a 0 fl container 1, and 100 β of an 84% aqueous ethanol solution was added thereto and stirred. This aqueous solution was sent to an ultrasonic stirring tank 3 using a circulation vibrator 2, and ultrasonic waves were irradiated using an ultrasonic nozzle 4 with an output of 200 W to destroy the cell membranes of chloroplasts and extract chlorophyll into the aqueous solution. This was returned to the container 1 again and stirred with an ultra high speed rotating blade 6 of about 6000 rpm.
Leave for 0 to 200 minutes. Thereafter, the contents in the container 1 are filtered to obtain 80 n of chlorophyll-containing ethanol solution as a mother liquor. The resulting fluid has a bright red color similar to human blood. Add this to more than 50% protein G (protein 79.0
%) and molded into granules using a vacuum concentrator. During this process, ethanol evaporates.
本発明の製法により得られた葉緑素製剤は、葉緑体の細
胞が破壊されているから葉緑素の体内への吸収がよく、
活性の葉緑素であり、しかも蛋白質との協力作用により
葉緑素の効果が奏される。In the chlorophyll preparation obtained by the production method of the present invention, chloroplast cells are destroyed, so chlorophyll is easily absorbed into the body.
It is an active chlorophyll, and the effects of chlorophyll are exerted by its cooperative action with proteins.
なお、母液として抽出された葉緑素含有エタノール液は
、室温約１８度の冷暗所に保存したところ、１年以上の
保存が可能であることが確認できた。In addition, when the chlorophyll-containing ethanol solution extracted as the mother liquor was stored in a cool, dark place at a room temperature of about 18 degrees, it was confirmed that it could be stored for more than one year.
第１図は本発明の製剤製法装置の例を示す概略図である
。
１：容器 ２：循環バイブ３：超音波攪拌
槽 ４：超音波ノズル５：超音波発振器 ６：
超高速回転羽根７；攪拌羽根 ８：循環ポンプ
９：原料投入口 ｌＯ：蓋
１１：残渣排出口FIG. 1 is a schematic diagram showing an example of the pharmaceutical manufacturing method apparatus of the present invention. 1: Container 2: Circulation vibrator 3: Ultrasonic stirring tank 4: Ultrasonic nozzle 5: Ultrasonic oscillator 6:
Ultra high speed rotating blade 7; Stirring blade 8: Circulation pump 9: Raw material inlet lO: Lid 11: Residue discharge port
Claims (1)
理を行い、得られた葉緑素抽出エタノール溶液と蛋白質
含有物とを練合することを特徴とする葉緑素製剤の製法
。 ２、超音波とともに超高速回転羽根による処理を行うこ
とを特徴とする請求項１記載の葉緑素製剤の製法。 ３、エタノール溶液が８４％以上の水溶液である請求項
１又は２記載の葉緑素製剤の製法。 ４、蛋白質含有物が蛋白質５０％以上の含有率を有する
ものである請求項１又は２記載の葉緑素製剤の製法。 ５、クロロフィル抽出エタノール溶液と蛋白質含有物の
混合割合が１：１〜１：３重量比である請求項１又は２
記載の葉緑素製剤の製法。[Scope of Claims] 1. A method for producing a chlorophyll preparation, which comprises treating a chlorophyll-containing substance in an ethanol solution with ultrasound, and kneading the obtained chlorophyll-extracted ethanol solution with a protein-containing substance. 2. The method for producing a chlorophyll preparation according to claim 1, characterized in that the treatment is carried out using ultra-high speed rotating blades in addition to ultrasonic waves. 3. The method for producing a chlorophyll preparation according to claim 1 or 2, wherein the ethanol solution is an 84% or more aqueous solution. 4. The method for producing a chlorophyll preparation according to claim 1 or 2, wherein the protein-containing material has a protein content of 50% or more. 5. Claim 1 or 2, wherein the mixing ratio of the chlorophyll extracted ethanol solution and the protein-containing substance is 1:1 to 1:3 by weight.
The method for producing the chlorophyll preparation described.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63236981A JPH0283329A (en) | 1988-09-20 | 1988-09-20 | Production of chloropyll preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63236981A JPH0283329A (en) | 1988-09-20 | 1988-09-20 | Production of chloropyll preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0283329A true JPH0283329A (en) | 1990-03-23 |
| JPH0348167B2 JPH0348167B2 (en) | 1991-07-23 |
Family
ID=17008624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63236981A Granted JPH0283329A (en) | 1988-09-20 | 1988-09-20 | Production of chloropyll preparation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0283329A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991003172A1 (en) * | 1989-09-07 | 1991-03-21 | Commonwealth Scientific And Industrial Research Organisation | Kiwifruit products |
| WO2002062161A1 (en) * | 2001-02-07 | 2002-08-15 | Susumu Takayama | Method and apparatus for producing health drink containing chlorophyll |
| WO2003015539A1 (en) * | 2001-08-13 | 2003-02-27 | Susumu Takayama | Method for producing beverage having chlorophyll |
| KR101040198B1 (en) * | 2007-11-30 | 2011-06-09 | 김동열 | the way of abstraction of chlorophyll |
| JP2016522171A (en) * | 2013-04-12 | 2016-07-28 | ウニベルシダーデ デ サンティアゴ デ コンポステラＵｎｉｖｅｒｓｉｄａｄ Ｄｅ Ｓａｎｔｉａｇｏ Ｄｅ Ｃｏｍｐｏｓｔｅｌａ | Antioxidant extracts from large brown algae and methods for obtaining them |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5933231A (en) * | 1982-08-19 | 1984-02-23 | Seikou Kin | Apparatus for extracting chlorophyll |
-
1988
- 1988-09-20 JP JP63236981A patent/JPH0283329A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5933231A (en) * | 1982-08-19 | 1984-02-23 | Seikou Kin | Apparatus for extracting chlorophyll |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991003172A1 (en) * | 1989-09-07 | 1991-03-21 | Commonwealth Scientific And Industrial Research Organisation | Kiwifruit products |
| WO2002062161A1 (en) * | 2001-02-07 | 2002-08-15 | Susumu Takayama | Method and apparatus for producing health drink containing chlorophyll |
| WO2003015539A1 (en) * | 2001-08-13 | 2003-02-27 | Susumu Takayama | Method for producing beverage having chlorophyll |
| KR101040198B1 (en) * | 2007-11-30 | 2011-06-09 | 김동열 | the way of abstraction of chlorophyll |
| JP2016522171A (en) * | 2013-04-12 | 2016-07-28 | ウニベルシダーデ デ サンティアゴ デ コンポステラＵｎｉｖｅｒｓｉｄａｄ Ｄｅ Ｓａｎｔｉａｇｏ Ｄｅ Ｃｏｍｐｏｓｔｅｌａ | Antioxidant extracts from large brown algae and methods for obtaining them |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0348167B2 (en) | 1991-07-23 |
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