JPH0149479B2 - - Google Patents
Info
- Publication number
- JPH0149479B2 JPH0149479B2 JP57093062A JP9306282A JPH0149479B2 JP H0149479 B2 JPH0149479 B2 JP H0149479B2 JP 57093062 A JP57093062 A JP 57093062A JP 9306282 A JP9306282 A JP 9306282A JP H0149479 B2 JPH0149479 B2 JP H0149479B2
- Authority
- JP
- Japan
- Prior art keywords
- bacteriophage
- dna
- endonuclease
- phage
- coli
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241001515965 unidentified phage Species 0.000 claims description 132
- 108020004414 DNA Proteins 0.000 claims description 75
- 108010042407 Endonucleases Proteins 0.000 claims description 71
- 102000004533 Endonucleases Human genes 0.000 claims description 71
- 238000003776 cleavage reaction Methods 0.000 claims description 46
- 230000007017 scission Effects 0.000 claims description 46
- 239000012634 fragment Substances 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 16
- 238000009395 breeding Methods 0.000 claims description 14
- 230000001488 breeding effect Effects 0.000 claims description 12
- 102000012410 DNA Ligases Human genes 0.000 claims description 9
- 108010061982 DNA Ligases Proteins 0.000 claims description 9
- 238000011144 upstream manufacturing Methods 0.000 claims description 9
- 230000002068 genetic effect Effects 0.000 claims description 8
- 102000003960 Ligases Human genes 0.000 claims description 3
- 108090000364 Ligases Proteins 0.000 claims description 3
- 238000013518 transcription Methods 0.000 claims description 3
- 230000035897 transcription Effects 0.000 claims description 3
- 101710132601 Capsid protein Proteins 0.000 claims description 2
- 101710094648 Coat protein Proteins 0.000 claims description 2
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims description 2
- 101710125418 Major capsid protein Proteins 0.000 claims description 2
- 101710141454 Nucleoprotein Proteins 0.000 claims description 2
- 101710083689 Probable capsid protein Proteins 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 description 59
- 239000000243 solution Substances 0.000 description 32
- 239000012137 tryptone Substances 0.000 description 29
- 229920001817 Agar Polymers 0.000 description 25
- 239000008272 agar Substances 0.000 description 25
- 239000002609 medium Substances 0.000 description 24
- 230000002101 lytic effect Effects 0.000 description 19
- 241000894006 Bacteria Species 0.000 description 16
- 108090000623 proteins and genes Proteins 0.000 description 15
- 238000012258 culturing Methods 0.000 description 13
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000003550 marker Substances 0.000 description 10
- 238000007796 conventional method Methods 0.000 description 7
- 238000002955 isolation Methods 0.000 description 6
- 108700025695 Suppressor Genes Proteins 0.000 description 5
- 230000036039 immunity Effects 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 229920000936 Agarose Polymers 0.000 description 4
- 241000701959 Escherichia virus Lambda Species 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 241001646716 Escherichia coli K-12 Species 0.000 description 3
- 241001302584 Escherichia coli str. K-12 substr. W3110 Species 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 230000014616 translation Effects 0.000 description 3
- 241000370685 Arge Species 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000001320 lysogenic effect Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 239000007160 ty medium Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 101150010882 S gene Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 230000001420 bacteriolytic effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/73—Expression systems using phage (lambda) regulatory sequences
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57093062A JPS58212781A (ja) | 1982-06-02 | 1982-06-02 | 新種バクテリオフア−ジ及びその育種法 |
| US06/498,802 US4865979A (en) | 1982-06-02 | 1983-05-27 | Novel bacteriophage and method for breeding thereof |
| EP83303125A EP0095934B1 (en) | 1982-06-02 | 1983-06-01 | Novel bacteriophage and method for breeding thereof |
| DE8383303125T DE3381989D1 (de) | 1982-06-02 | 1983-06-01 | Bakteriophage und verfahren zur zuechtung. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57093062A JPS58212781A (ja) | 1982-06-02 | 1982-06-02 | 新種バクテリオフア−ジ及びその育種法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58212781A JPS58212781A (ja) | 1983-12-10 |
| JPH0149479B2 true JPH0149479B2 (enExample) | 1989-10-24 |
Family
ID=14072023
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57093062A Granted JPS58212781A (ja) | 1982-06-02 | 1982-06-02 | 新種バクテリオフア−ジ及びその育種法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4865979A (enExample) |
| EP (1) | EP0095934B1 (enExample) |
| JP (1) | JPS58212781A (enExample) |
| DE (1) | DE3381989D1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61274683A (ja) * | 1985-05-29 | 1986-12-04 | Kikkoman Corp | ベクタ− |
| US6284492B1 (en) | 1988-02-26 | 2001-09-04 | Large Scale Biology Corporation | Recombinant animal viral nucleic acids |
| US6054566A (en) | 1988-02-26 | 2000-04-25 | Biosource Technologies, Inc. | Recombinant animal viral nucleic acids |
| JPH03502886A (ja) * | 1988-02-26 | 1991-07-04 | バイオソース・ジェネティクス・コーポレイション | 非核染色体形質転換 |
| US5316931A (en) * | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
| US5427908A (en) * | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
| CA2186962A1 (en) * | 1994-04-05 | 1995-10-12 | Richard M. Carlton | Antibacterial therapy with genotypically modified bacteriophage |
| US20010043924A1 (en) * | 1994-04-05 | 2001-11-22 | Exponential Biotherapies, Inc. | Antibacterial therapy with bacteriophage physico-chemically altered to delay inactivation by the host defense system |
| US5741697A (en) * | 1995-11-30 | 1998-04-21 | University Of Rochester | Bacteriophage of chlamydia psittaci |
| JPH10108679A (ja) * | 1996-10-03 | 1998-04-28 | Kikkoman Corp | ピルベートオルトフォスフェートジキナーゼ遺伝子、組み換え体ｄｎａ及びピルベートオルトフォスフェートジキナーゼの製造法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS53133684A (en) * | 1977-04-26 | 1978-11-21 | Noda Sangyo Kagaku Kenkyusho | Novel bacteriophage and fabricating same |
| FR2422685A1 (fr) * | 1978-04-14 | 1979-11-09 | Pasteur Institut | Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli |
| JPS5558096A (en) * | 1978-10-25 | 1980-04-30 | Noda Sangyo Kagaku Kenkyusho | Method of making novel recombined dna |
| JPS5561798A (en) * | 1978-10-30 | 1980-05-09 | Noda Sangyo Kagaku Kenkyusho | Preparation of novel recombination dna |
| JPS5835198A (ja) * | 1981-08-28 | 1983-03-01 | Mitsubishi Petrochem Co Ltd | 新規ベクター |
| JPS5869898A (ja) * | 1981-10-23 | 1983-04-26 | Noda Sangyo Kagaku Kenkyusho | 新規なテンペレ−トフア−ジｄｎａ |
-
1982
- 1982-06-02 JP JP57093062A patent/JPS58212781A/ja active Granted
-
1983
- 1983-05-27 US US06/498,802 patent/US4865979A/en not_active Expired - Lifetime
- 1983-06-01 DE DE8383303125T patent/DE3381989D1/de not_active Expired - Lifetime
- 1983-06-01 EP EP83303125A patent/EP0095934B1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS58212781A (ja) | 1983-12-10 |
| EP0095934A3 (en) | 1985-08-14 |
| EP0095934B1 (en) | 1990-11-14 |
| EP0095934A2 (en) | 1983-12-07 |
| DE3381989D1 (de) | 1990-12-20 |
| US4865979A (en) | 1989-09-12 |
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