JPH0143551B2 - - Google Patents
Info
- Publication number
- JPH0143551B2 JPH0143551B2 JP11212981A JP11212981A JPH0143551B2 JP H0143551 B2 JPH0143551 B2 JP H0143551B2 JP 11212981 A JP11212981 A JP 11212981A JP 11212981 A JP11212981 A JP 11212981A JP H0143551 B2 JPH0143551 B2 JP H0143551B2
- Authority
- JP
- Japan
- Prior art keywords
- urokinase
- adsorbent
- agarose
- separating
- conductivity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 claims description 59
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 claims description 59
- 229960005356 urokinase Drugs 0.000 claims description 58
- 238000000034 method Methods 0.000 claims description 22
- 239000003463 adsorbent Substances 0.000 claims description 21
- 229920000936 Agarose Polymers 0.000 claims description 11
- 238000001179 sorption measurement Methods 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 24
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 15
- 230000000694 effects Effects 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 102000004169 proteins and genes Human genes 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- 238000005406 washing Methods 0.000 description 9
- 239000002158 endotoxin Substances 0.000 description 7
- 229920002684 Sepharose Polymers 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 239000007853 buffer solution Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000011067 equilibration Methods 0.000 description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 3
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 3
- 239000008351 acetate buffer Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 239000002510 pyrogen Substances 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 229940041181 antineoplastic drug Drugs 0.000 description 2
- 239000003527 fibrinolytic agent Substances 0.000 description 2
- 230000003480 fibrinolytic effect Effects 0.000 description 2
- VANNPISTIUFMLH-UHFFFAOYSA-N glutaric anhydride Chemical compound O=C1CCCC(=O)O1 VANNPISTIUFMLH-UHFFFAOYSA-N 0.000 description 2
- -1 hydroxyaminopropyl Chemical group 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 235000019362 perlite Nutrition 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229940014800 succinic anhydride Drugs 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 241000239218 Limulus Species 0.000 description 1
- 102000013566 Plasminogen Human genes 0.000 description 1
- 108010051456 Plasminogen Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 238000011046 pyrogen test Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11212981A JPS5813390A (ja) | 1981-07-20 | 1981-07-20 | 高純度ウロキナ−ゼの分離精製法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11212981A JPS5813390A (ja) | 1981-07-20 | 1981-07-20 | 高純度ウロキナ−ゼの分離精製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5813390A JPS5813390A (ja) | 1983-01-25 |
| JPH0143551B2 true JPH0143551B2 (enrdf_load_stackoverflow) | 1989-09-21 |
Family
ID=14578917
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11212981A Granted JPS5813390A (ja) | 1981-07-20 | 1981-07-20 | 高純度ウロキナ−ゼの分離精製法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5813390A (enrdf_load_stackoverflow) |
-
1981
- 1981-07-20 JP JP11212981A patent/JPS5813390A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5813390A (ja) | 1983-01-25 |
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