JPH01172340A - Preventive and remedy for staphylococcal disease of chicken - Google Patents

Abstract

PURPOSE:To obtain the title preventive and remedy having no problems of occurrence of resistant bacteria and side effects and no fear of influence of remaining agent on human body, comprising a crude drug such as root and rhizome of Glycyrrhiza glabra Linne var. glandulifera Regel et Herder, rhizome of Coptis japonica Makino or root of Scutellarla baicalensis Georgi as an active ingredient. CONSTITUTION:The title preventive and remedy comprising a crude drug selected from the group consisting of root and rhizome of Glycyrrhiza glabra Linne var. glandulifera Regel et Herder, rhizome of Coptis japonica Makino, root of Scutellarla baicalensis Georgi, bark of Phellodendron amurense Ruprecht, bark of Magnolia obovata Thumberg, root of Salvia milltiorrhiza Bunge, rhizome of Rheum palmatum Linne, rhizome of Anemanhena asphodeloides Bunge, bud of Eugenia caryophyllata Thumb., root of Sophora augustifolia Siebold et Zuccarini, dried bark of Cinna momum Cassla Blume, leaves of Eriobotrya japonica Lindely, fruit ear of Humulus Lupulus Linne, bark of Myrica rubra Siebold et Zuccarini, stems and leaves of Isodon japonica Hara, Galla rhois, fruit of Cornus officinalis Siebold et Zuccarini, root of Paeonia albiflora Pallas var. trichocarpa Bunge, dried root of Sanguisorba officinalis L. and ground stem of Ephedra sinica Stapf as an active ingredient. The crude drug is administered in the form of raw powder, essence extracted with an organic solvent or water or an active ingredient of Galla rhois obtained from the essence and a dose thereof is 0.001mg-100g calculated as weight of raw powder per kg weight daily.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a prophylactic and therapeutic agent and a method for preventing and treating staphylococcal disease in chickens.

More specifically, the present invention relates to preventive and therapeutic agents containing crude drugs for staphylococcal disease in chickens, and preventive and therapeutic methods comprising administering crude drugs.

[Prior Art] Staphylococcus disease in chickens is caused by staphylococci, namely Staphylococcus aureus 5 taphylococcusaur.
This is a disease caused by infection with Eus. It is one of the most important diseases of chickens for which it is urgently necessary to establish control measures, as both the incidence and mortality rate are high and the industrial damage it causes to poultry farming and other industries is significant.

This disease causes a wide variety of illnesses, including edematous dermatitis, which is unique to this disease. Examples include hyaluronic aneurysm, dry scarring at the heel end, and endocarditis. In addition, mixed infections with this bacterium and other pathogenic bacteria are very common.

Despite the fact that it is such a serious infectious disease, there are almost no effective countermeasures, and although measures such as proper breeding management are taken, in reality it is often impossible to prevent it.

Furthermore, in order to prevent and treat this disease, for example, methods have been used in which antibiotics and other antibacterial agents are administered together with feed. However, in recent years, there has been a growing trend that it is not appropriate to administer antibiotics to poultry, etc., particularly due to the emergence of resistant bacteria and the persistence of antibiotics in animals. Therefore, the development of preventive and therapeutic methods and preventive and therapeutic agents that do not use antibiotics is desired.

[Means for Solving the Problems] As a result of research to solve the above problems, the present inventors discovered that certain herbal medicines have the effect of suppressing this disease, and completed the present invention. Ta.

That is, the purpose of the present invention is to provide a preventive and therapeutic agent for staphylococcal disease in chickens, as well as a preventive and therapeutic method according to the same agency.

The present invention provides licorice, orensis, scutellariae, scutellariae,
Chicken containing one or more herbal medicines selected from the group consisting of Kobok, Danjin, Chimo, Rhubarb, Clove, Kujin, Keihi, Biwayo, Hop, Ibaraki, Chimney root, Gobaishi, Cornelia, Peony, Chuyu, and Ephedra. The present invention provides a preventive and therapeutic agent for staphylococcal disease.

The present invention also provides for the prevention and treatment of staphylococcal disease in chickens, which contains crude drugs in the form of bulk powders, organic solvents, mixtures of organic solvents and water, or crude drug extracts obtained by extraction with water, or crude drug active ingredients. It provides a therapeutic agent.

The present invention also provides a prophylactic and therapeutic agent for staphylococcal disease in chickens, which is prepared by incorporating crude drugs into feed.

In addition, the present invention provides licorice, orensis, scutellariae, scutellariae, scutellariae, tanjin, chimo, rhubarb, clove,
Staphylococcus disease in chickens comprising administering an effective amount of one or more crude drugs selected from the group consisting of Kujin, Keihi, Biwayo, hops, Japanese apricot, Japanese cornstarch, Gobaishi, cornelian cornelian, peony, chiyu, and ephedra. The present invention provides a method for prevention and treatment of.

The present invention also provides a method for staphylococcal disease in chickens, which comprises administering a crude drug in the form of a bulk powder, an organic solvent, a mixture of an organic solvent and water, or a crude drug extract obtained by extraction with water, or in the form of an active ingredient of a crude drug. The present invention provides a method for prevention and treatment of.

The present invention also provides a method for preventing and treating staphylococcal disease in chickens, which comprises administering a crude drug in feed.

Licorice (licorice) is
aglabra Linnc var, grand
ulifera Regel at Herder) roots and rhizomes as they are or with the cork skin removed. This product contains ingredients such as glycyrrhizin, liquiritin, glucose, and asparagine, and is used as a relaxation, antispasmodic, and sputum medicine for humans.

Ouren (Yellow Ren) is ouren (Coptlsjapo
The fine roots are burned off from the rhizome of Nlca Maklno). This product contains ingredients such as berberine and palmatine, and is used as a bitter and intestinal medicine for humans.

Scutel la
This is the root of a plant (Riabaicalcnsis Gcorgi) with the cork skin removed. This product contains orgonin and picalin as ingredients, and is used as an anti-inflammatory and antipyretic drug for humans.

Yellowfin tuna (Pheel Iodend)
ronamurense Rupreeht) or other cultivated plants, with the cork layer removed. This product contains berberine, palmatine, etc. as ingredients, and is used as a stomachic, intestinal regulation, astringent, and anti-inflammatory drug for humans.

Kouboku (Koboku) is Magnoliaob (Magnoliaob).
It is the bark of the tree and branches of Thunberg ovata. This product contains magnoclarine and essential oils whose main components are matyrol and magnolol, and is used as an astringent diuretic and phlegm medicine for thoracic and abdominal distension, abdominal pain, and wheezing cough in humans.

Dangjin (Danjin) is Dangjin (5alviaIIilt)
lorrhiza Bungc). This product contains tankudin I, 1, and cryptotankdin as ingredients, and is used as a tonic, menstrual, and gynecological medicine for menstrual disorders, uterine bleeding, abdominal pain, and various healing properties.

Chimo (Chimo) is Anemanhenaas
phodeloides Bunge).

This product contains saponin, steroid saponin,
Contains tannins, etc., and is used as an anti-inflammatory, antipyretic, warm-temperature, diuretic, and analgesic in humans.

Rhubarb (Rheum spp.) is Rheu layer pa1ma
tuIIL1nn6 or R,ofTlcinal
The rhizomes of the rhizomes (e. Balllon) and related plants are vertically divided with most of the bark removed. This product contains chrysophanol as an anthraquinone derivative,
Contains emodin, emodin monomethyl ether, etc., and is used as a bitter, laxative, and anti-inflammatory drug for chronic gastrointestinal diseases, indigestion, and constipation.

Clove is clove (Eugeniacar).
yophyllata Thunb,) just before flowering. This product contains components such as eugenol and acetyl eugenol, and is used to promote human digestive function, as an anthelmintic, and as a spice and cosmetic.

Chryso (bitter ginseng) is Clara (Sophora augus)
tifolla 51ebold et Zuccar
inl) root intact or cut transversely or vertically with most of the cork skin removed. This product contains matrine as a component and is used as a stomachic, diuretic, antipyretic, analgesic, and anthelmintic for humans.

Cinnamon sosum
Cassia Blu*e) is dried and treated, with the cork layer removed to some extent. As an ingredient, this
Contains cinnamaldehyde, cinnamyl acetate, etc., and is used as a sweating, antipyretic, analgesic, stomachic, intestinal regulation, and anthelmintic drug for humans.

Loquat (Erlobotryaj) is Loquat (Erlobotryaj).
aponlca Llndley) from which the hairs of the leaves have been removed as much as possible. This substance is glucose, sucrose,
Containing fructose, maltose, etc. as ingredients, it is used as a refreshing and stomachic medicine for humans and as a preventative against hot weather.

Hops are hops (Humulus Lupulus)
It is the mature fructose of Llnn6). This product contains tannins, tumurone, rubron, etc. as components, and is necessary for imparting bitterness and aroma in beer brewing.

Youbaihi (fried plum skin) is a Japanese bayberry (Myricaru).
bra 5iebold at Zuccarini)
It is the bark of the tree. This product contains ingredients such as tannin, myricetin, and myricitrin, and is used as an astringent for diarrhea in humans, and is also effective for bruises.

Enmeiso (Enmeiso) is called Hikiokoshi (Isodon).
japonica Hara) or lsodon trichocarpus Ku
These are the stems and leaves of do).

This product contains plectrantin and enmain as ingredients, and is used as a bitter medicine for human stomach health to treat indigestion, anorexia, abdominal pain, etc.

Gobaishi (Gobaishi) is the aphid (Nurudenomimifushia aphid)
Melaphls chinensls J, Be1
The sting of Rhus java
It is a gall produced on the leaves of Nlca Linne) and contains tannin, gallic acid, fat, resin, etc. as components. It is used as a raw material for the production of five-fold tincture, tannic acid, gallic acid, and pyrogallol.

Cornuso (Japanese cornflower)
1'f'1clnalls 5iebold eL Z
Seeds are extracted from the mature fruits of uccarlnl). This product contains crystalline organic acids, gallic acid, malic acid, tartaric acid, etc. as ingredients. Human nourishment, tonicity,
It is used as an astringent.

Peony (Xuyaku) is Peony (Paeon1aa)
lblf'1ora Pa1las var, tri
chocarpa Bunge). This product contains benzoic acid, resin, etc. as ingredients, and has an astringent effect on humans.
It is used as a relaxing, analgesic, and antispasmodic.

Chiyu (earth elm) is Sanguisorba
of Tlclnalfs L,) was taken and dried. This product contains tannins (17
%), contains saponin, and is used as a hemostatic astringent in humans to treat hematemesis, hemoptysis, and menorrhagia.

Ephedra (Ephedra 51n1)
caStapr) above ground stem. This product contains components such as g-ephedrine and d-pseudoephedrine, and is used as an antitussive, diaphoretic, and diuretic for humans.

As mentioned above, the herbal medicines used in the present invention include licorice, orensis, scutellariae, scutellariae, scutellariae, tanjin, chimo, rhubarb, clove, kujin, kehihi, loquat, hop, scutellariae, japonica, burdock, cornelian, peony, chiyu, and ephedra. Each of these has been conventionally used alone or in combination for the prevention and treatment of human diseases. However, no attempt has been made to use these for the prevention and treatment of diseases in chickens, particularly for the prevention and treatment of infectious diseases in chickens.

The present inventors focused on the medicinal effects of the above-mentioned herbal medicines and as a result of various studies, we found that staphylococcal disease in chickens, that is, bacterial 5taphylo.
The present invention was completed based on the discovery that administering one or more of the above-mentioned herbal medicines in combination is extremely effective for preventing and treating diseases caused by infection with coccus aureus.

That is, as described below, the above-mentioned specific types of herbal medicines and their extracts have been shown to be effective against bacterial 5t in vitro and in vivo.
It has been revealed that it has bacteriostatic and bactericidal effects specifically against Aphylocoecus aurcus.

And this 5taphylococeus aureu
As a result of the inhibitory effect on S., when these herbal medicines were administered to chickens, it was possible to prevent and cure staphylococcus disease, a chicken disease caused by Taphylococcus aureus.

The effectiveness of these herbal medicines and their extracts against diseases specific to chickens was unexpected and was revealed for the first time by the present inventors.

These crude drugs can be administered directly in the form of raw powder, extracts, or active ingredients of crude drugs, or they can be added to feed, or solutions, dispersions, semi-solids, powders, and molded preparations. It is used in formulations such as infusion preparations, injection preparations, etc.

Extracts can be obtained by solvent extraction, for example using water, an organic solvent, or a mixture of water and an organic solvent, and then left as is, or
It can be used concentrated, diluted, or with the solvent removed.

Examples of organic solvents include methanol, ethanol, n
-Propertool, n-butanol, acetone, ethyl acetate, ether, methylene chloride, chloroform, benzene, carbon tetrachloride, petroleum ether, etc. are used, and methanol and ethanol are particularly preferred. These organic solvents can be used alone or as a mixture of two or more.

This solvent extraction can be carried out by cold soaking or digesting the herbal medicine in these solvents. 15-25℃ for cold soaking
Digestion can be carried out at a temperature range of 35-45°C. The extraction time will vary depending on the extraction temperature, but may be approximately 5 days or until the soluble components are fully dissolved in the shell.

The active ingredients of herbal medicines can be obtained by, for example, shaking the above-mentioned extracted extract using various solvents, collecting the fraction that transfers to the solvent, and distilling off the solvent, which can be used as the effective fraction, that is, the active ingredient. I'll come.

The solvent used in this case is a non-polar solvent such as lower aliphatic ethers (diethyl ether, ethyl methyl ether, etc.) - lower halogen alkanes (chloroform, ethyl methyl ether, etc.).
Examples include dichloroethane, dibromoethane, etc.); benzenes (benzene, toluene, xylene, etc.); and other petroleum solvents (petroleum ether, petroleum benzene, ligroin, etc.).

In addition, the remaining aqueous solution from which this effective fraction was extracted is extracted by shaking using water and a kind of organic solvent saturated with water, washed with water, and transferred to the organic solvent layer that is the residue. A product obtained by distilling off both solvents can also be used as an effective fraction, that is, as an active ingredient. This effective fraction has different physicochemical properties and different detected components from the above-mentioned effective fraction.

The dose is 0.001 in terms of crude drug bulk powder I￥rJl.
mg/body ff1kg/day to 100 g/body weight) cg/day.

This amount is different when used as a prophylactic agent and as a therapeutic agent, with the latter being a higher dose in the shell.

The administration method can be oral or parenteral administration, and examples of parenteral administration include intramuscular administration, intraperitoneal administration, transdermal administration, nasal administration, intravenous administration, and the like.

[Effects of the Invention] As detailed above, the present invention provides an effective preventive and therapeutic agent and an effective preventive and therapeutic method for staphylococcal disease in chickens.

Since the prophylactic and therapeutic agent of the present invention uses herbal medicines or extracts obtained from them as herbal medicines, there is no problem of the development of resistant bacteria or side effects associated with antibiotics, etc., and it can be administered with confidence.

In addition, since there is no concern about the effects of drug residue on the human body, it is suitable as a drug for preventing and treating staphylococcal disease in egg-producing chickens and meat chickens.

Furthermore, the disease can be prevented and treated at a lower cost than when using antibiotics or the like.

The present invention will be explained in more detail below using test examples and examples.

Test Example 1 The sensitivity of Taphylococcus aureus to various crude drugs was investigated.

Various crude drugs were added to a 50% aqueous ethanol solution in an amount seven times the amount of the crude drugs, and extracted for 16 hours to obtain alcoholic extracts.

The extract was infiltrated into a 8III diameter disk in 25 μm increments and dried. This disk (Staphyl
ococcus aurcus) strain.
After placing on an infusion agar medium and culturing at 87°C for 18 hours, the diameter of the 11-circle was measured.

Next, the test results are shown in Table 1.

(Leaving space below) Table 1 Daylily 12 Daylily 15 Daylily 12 Daylily 12 Daylily 12 Daylily 12 Daylily 15 Daylily
Mo 1B rhubarb
9 Choji 12ri
Gin 12 pieces
I Hi 11 Beyond
9 Hop 11 Youbaihi 12 Enmeiso 16 Gobaishi 16 Table 1 (Continued) Cornelius 10 Peony 10 Chu 11 Mao
C. 11 Example 1 Basic feed corn 402 parts by weight Milo
240
40 Soybean meal
16 o〃na lees
4o〃Fish powder
35 ~Yellow grease 7 Calcium carbonate 7o Tertiary calcium phosphate 2 parts by weight
Salt 2 Premix 2 Licorice powder was added at 4% by weight to the basic feed to prepare a test feed.

Twenty healthy chickens, 150 days old, were fed the test feed ad libitum. and protease-producing 5taphyloc isolated from chickens with staphylococcus infection on the 5th day from the start of the test.
occus aureus at 108 pieces/ml per chicken.
1 ml was administered subcutaneously to the right side of the head. Next, 20 days after administration, swelling at the inoculation site, autopsy findings, and bacteria collection from the inoculation site were performed.

In the control group, the basal feed was given instead of the test feed.

As a result, among the chickens after infection, swelling at the inoculation site was observed in 5 chickens in the test group of the present invention, but 18 chickens in the control group.

Further, according to autopsy findings, cheese-like substances were observed at the inoculation site in 5 birds in the test group of the present invention, but in 18 birds in the control group. Furthermore, when collecting bacteria from the inoculation site, only 5 birds were recovered in the test area, but 18 in the control area.
Bacteria were recovered from the feathers.

Examples 2 to 20 The same procedure as in Example 1 was conducted except that 1% of the crude drug shown in Table 2 below was added instead of licorice.

Next, the test results are shown in Table 2.

(Margins below) Example 21 Daylily, Oren, Scutellariae, Scutellariae, Cucumber,
We took 1 kg of each of Danjin, Chimo, Rhubarb, Clove, Kujin, Keihi, Biwayo, Hops, Japanese radish, Chimney sour, Gobaishi, Cornus trifolium, Peony, Chuyu, and Ephedra, and each was added to ION water and extracted at 100°C for 1 hour. .

Each water extract was diluted 1000 times to obtain an extract.

Ten 150-day-old chickens were used, and the extract was fed in place of water. The same basal feed as that used in Example 1 was used as the feed. In the control group, water was given instead of the extract. And protease-producing 5Laphyloeos isolated from chickens with staphylococcus infection on the fifth day from the start of the test.
ccus aureus at 108 pieces/ml per bird.
ml was administered subcutaneously to the right side of the head for infection, and then reared for 20 mouths in the same manner as before infection.

As a result, the number of chickens showing swelling of the inoculation site in the eye after infection, the number of chickens with cheese-like substance at the inoculation site in necropsy findings, and the number of chickens in which bacteria were recovered were compared in all test plots. It was better than the ward.

Claims (1)

[Scope of Claims] 1) Licorice, orensis, scutellariae, scutellariae, scutellariae, tanjin, chimo, rhubarb, clove, kujin, cinnamon hive, loquat, hop, scutellariae, japonica,
A prophylactic and therapeutic agent for staphylococcal disease in chickens, which contains one or more crude drugs selected from the group consisting of Japanese cabbage, cornelian cornflower, peony, citrus and ephedra. 2) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is in the form of bulk powder. 3) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is an organic solvent, a mixture of water and an organic solvent, a crude drug extract extracted with water, and/or a crude drug active ingredient obtained therefrom. 4) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is blended into feed. 5) Licorice, orensis, scutellariae, scutellariae, scutellariae, tanjin, chimo, rhubarb, clove, kujin, kehihi, loquat, hop, scutellariae,
A method for preventing and treating staphylococcal disease in chickens, which comprises administering an effective amount of one or more herbal medicines selected from the group consisting of burdock, cornelian, peony, citrus and ephedra. 6) The prevention and treatment method according to claim 5, wherein the crude drug is used in the form of bulk powder. 7) Herbal medicines are organic solvents,
The prevention and treatment method according to claim 5, which is a mixture of water and an organic solvent or a crude drug extract extracted with water and/or a crude drug active ingredient obtained therefrom. 8) The prevention and treatment method according to claim 5, wherein the crude drug is administered by mixing it with feed.