JPH01172334A - Preventive and remedy for swine pasteurellosis - Google Patents

Abstract

PURPOSE:To obtain the title preventive and remedy having no problems of occurrence of resistant bacteria and side effects and no fear of influence of remaining agent on human body, comprising a crude drug such as rhizome of Rheum palmatum Linne, rhizome of Coptis japonica Makino or bark of Phellodendron amurense Ruprecht as an active ingredient. CONSTITUTION:The title preventive and remedy comprising a crude drug selected from the group consisting of rhizome of Rheum palmatum Linne, rhizome of Coptis japonica Makino, bark of Phellodendron amurense Ruprecht, root of Scutellarla bacialensis Georgi, stems and leaves of Geranlum nepalense Sweet, root and rhizome of Glycyrrhiza glabra Linne var. glanduliffera Regel et Herder, stems and leaves of Isodon japonica Hara, dried root of Sanguisorba officinalis L., fruit ear of Humulus Lupulus Linne, gall of Bostaurus domesticus Gmelin, fruit of Cornus officinalis Siebold et Zuccarini, Galla rhois, root of Salvia miltiorrhiza Bunge, rhizome of Picrorrhiza Kurooa Royle ex Benth, Aloe arborescens Mill. var. natalensis Bergel., bud of Eugenia caryophyllata Thumb. and unripe fruit of Prunus Mume Siebold et Zuccarini as an active ingredient. The crude drug is administered in the form of raw powder, essence extracted with an organic solvent or water or an active ingredient of Galla rhois obtained from the essence and a dose thereof is 0.001mg-100g calculated as weight of raw powder per kg weight daily.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a prophylactic and therapeutic agent and a prophylactic and therapeutic method for pasteurellosis in pigs.

More specifically, the present invention relates to the bacterium Pa5teurel.
The present invention relates to preventive and therapeutic agents and preventive and therapeutic methods using crude drugs for pasteurellosis, which is an infectious disease of pigs caused by IaultOc1da.

[Prior art] Pasteurellosis of pigs is one of the bacterial infectious diseases of pigs, and the pathogen is Pa5teurella 5ultoclda ◎Since both the incidence and mortality rate are high and the industrial damage caused to the pig farming industry is large, Among the many diseases in the world, it is one of the most important diseases for which it is urgently necessary to establish control measures.

This disease is mainly caused by pneumonia. Symptoms are similar to those of Haemophilus pneumonia, and mixed infections may occur. This bacterium can originally exist in the upper respiratory tract mucosa of healthy pigs, but it becomes pathogenic when the animal is exposed to some kind of trigger, such as a mild respiratory tract infection or long-distance transport. .

In addition, secondary infections may occur due to other respiratory diseases such as epidemic pneumonia and atrophic rhinitis.

In acute cases of this disease, the onset temperature is 40-42℃.
Fever is present, and many patients die within a few days of onset.

Despite the fact that it is such a serious infectious disease, there are almost no effective countermeasures, and although measures such as proper breeding management are taken, in reality it is often impossible to prevent it.

Furthermore, in order to prevent and treat this disease, for example, methods have been used in which antibiotics and other antidepressants are administered together with feed. However, in recent years, there has been a growing trend that it is not appropriate to administer antibiotics to animals such as livestock due to problems such as the emergence of resistant bacteria and the persistence of antibiotics in animals. Therefore, it is desired to develop methods and therapeutic agents for preventing and treating chickenpox without using antibiotics.

[Means for Solving the Problems] As a result of research to solve the above problems, the present inventors discovered that certain herbal medicines have the effect of suppressing this disease, and completed the present invention. Ta.

That is, an object of the present invention is to provide a preventive and therapeutic agent for pasteurellosis in pigs, as well as a preventive and therapeutic method according to the same agency.

The present invention relates to rhubarb, rhubarb, rhubarb, rhubarb, scutellariae,
Prevention of pasteurellosis in pigs containing one or more herbal medicines selected from the group consisting of Gennoshoko, Licorice, Trifolium, Chiyu, Hop, Gyutan, Cornelia, Gobaishi, Danjin, Kooren, Aloe, Clove and Ubai. It provides a therapeutic agent.

The present invention also provides the prevention and treatment of pasteurellosis in pigs containing the herbal medicine in the form of a bulk powder, an organic solvent, a mixture of an organic solvent and water, or a herbal medicine extract obtained by extraction with water, or an active ingredient of the herbal medicine. The purpose is to provide an agent for

The present invention also provides a preventive and therapeutic agent for pasteurellosis in pigs, which is prepared by incorporating a herbal medicine into feed.

Furthermore, the present invention relates to rhubarb, orientalis, orientalis, orientalis, orientalis, licorice, orchid,
A method for the prevention and treatment of pasteurellosis in pigs, which comprises administering an effective amount of IFJ or two or more crude drugs selected from the group consisting of hops, gyutan, cornelian, gobaishi, tanjin, kouji, aloe, clove, and ubai. This is what we provide.

The present invention also relates to pasteurella in pigs, which is administered in the form of a crude drug in the form of a bulk powder, an organic solution, an agent, a mixture of an organic solvent and water, or a crude drug extract obtained by extraction with water, or in the form of an active ingredient of a crude drug. The aim is to provide methods for preventing and treating the disease.

The present invention also provides a method for preventing and treating pasteurellosis in pigs, which comprises administering a crude drug in feed.

Rhubarb (Rheu pa1ma) is Rheu pa1ma.
tum Linne or R, of'f'1cln
Ale Balllon) and related plants are cut vertically with most of the skin removed. This product contains chrysophanol, emodin, emodin monomethyl ether, etc. as anthraquinone derivatives,
It is used in humans as a bitter, laxative, and anti-inflammatory drug to treat chronic gastrointestinal diseases, indigestion, and constipation.

Ouren (Yellow Ren) is Ouren (CoptlsJapo)
The fine roots are burned off from the rhizome of N.Ica Maklno. This product contains berberine, nocilmatine, etc. as ingredients, and is used as a bitter and intestinal medicine for humans.

Yellowfin tuna (Pheel Iodend)
ronasurense Ruprecht) or other cultivated plants, with the cork layer removed. This product contains berberine, palmatine, etc. as ingredients, and is used as a stomachic, intestinal regulation, astringent, and anti-inflammatory drug for humans.

Scutellariae (Seutel Ia)
rlabalcalensis Georgi) from which the cork skin has been removed. The ingredients of this product are ogonin and picalin, and it is used as an anti-inflammatory and antipyretic drug in humans.

Gen no Shoko is Gen no Shoko (Geranlum).
nepalense Sweet) in summer. This product contains tannins, gallic acid, succinic acid, etc., and is used as an astringent for humans to treat diarrhea and abdominal pain.

Licorice (licorice) is
aglabra Linne var, grand
ul('era regel etllerder)
The roots and rhizomes are either intact or with the cork skin removed. This product contains glycyrrhizin, liquiritin, glucose, asparagine, etc. as ingredients, and has a soothing effect on humans.
It is used as an antispasmodic and sputum medicine.

Enmeiso (Enmeiso) is called Hikiokoshi (Isodon).
japonica Hara) or Isodon trichocarpus Ku
These are the stems and leaves of do).

This product contains plectrantin and enmain as ingredients, and is used as a bitter medicine for human stomach health to treat indigestion, anorexia, abdominal pain, etc.

Chiyu (earth elm) is Sangulsorba
oITlclnalls L.) was harvested and dried. This product contains tannins (17
%), contains saponin, and is used as a hemostatic astringent in humans to treat hematemesis, hemoptysis, and menorrhagia.

Hops are hops (Llumulus Lupulus)
This is a mature fruit panicle of L1nn6). This product contains tannins, tumuron, rubron, etc. as ingredients, and is necessary for imparting bitterness and aroma in beer brewing.

Gyutan (cow intestine) is a bovine intestine.
The gallbladder of G. mesticus Gzelin is hung to dry in the shade, or the bile duct is incised and the bile is stored in a container in a sealed container. As an ingredient, this
It contains sodium cholate, mucoprotein, fatty acids, etc., and has been used for a long time to treat yellow scars, constipation, diabetes, hemorrhoids, etc. in humans.

Cornuso (cornuso)
f'f'1clnalis 5iebold et Z
Seeds are extracted from the mature fruits of P. ucearini). This product contains crystalline organic acids, gallic acid, malic acid, tartaric acid, etc. as ingredients, and is nourishing and tonic for humans.
It is used as an astringent.

Gobaishi (Gobaishi) is the aphid (Nurudenomimifushia aphid)
Melaphls chlnensls J, Be1
The sting of Rhus java
It is a gall produced on the leaves of Nica Linne, and contains tannin, gallic acid, fat, resin, etc. as components. It is used as a raw material for the production of five-fold tincture, tannic acid, gallic acid, and pyrogallol.

Dangjin (Danjin) is Dangjin (SalvIaa+1lt
iorrhiza Bunge). This product contains tandinone I, II, and cryptotandinone as ingredients, and is used for human tonicity, menstruation, and as a feminine aid for menstrual disorders, uterine bleeding, abdominal pain, and various types of chiasma.

Plcrorrhiza Kurooa Royle is Plcrorrhiza Kurooa Royle.
ex Benth,). This product contains ingredients such as kutokin, D-mannitol, and vanillic acid, and is used as a bitter stomachic and laxative for humans.

Aloe is Aloe arborescens.
Mllll.

var, natalensls Bergel,) is boiled down. This product contains barbaroin, aloe emodin, etc. as ingredients, and has been used as a bitter stomachic or laxative for a very long time in the Middle East and Europe.

Clove is clove (Eugeniacar).
yophyllata Thunb,) just before flowering. This product contains components such as eugenol and acetyl eugenol, and is used to promote human digestive function, as an anthelmintic, and as a spice and cosmetic.

Prunus Muse 5
The fruit is peeled off from the unripe fruit of A. ieboldet Zuccarlnl and buried in soot to dry.

This product contains ingredients such as succinic acid, citric acid, malic acid, and tartaric acid, and is used as a refreshing astringent, hot water, insect repellent, antipyretic, etc. for humans.

As mentioned above, each of the crude drugs used in the present invention, rhubarb, orensis, aspergillus, aspergillus, gennoshoko, licorice, daylily, chiyu, hop, gyutan, cornelian, gobaishi, tanjin, kooren, aloe, clove, and ubai Traditionally used alone or in combination for the prevention and treatment of human diseases, they are now being used for the prevention and treatment of diseases in pigs, especially for the prevention and treatment of infectious diseases in pigs. No attempt has ever been made to use it for therapeutic purposes.

The present inventors conducted various studies focusing on the medicinal effects of the above-mentioned herbal medicines, and as a result, we found that pigs with pasteurellosis, that is, pigs are infected with bacterial Pa5.
The present invention was completed based on the discovery that it is extremely effective to administer one or more of the above-mentioned crude drugs in combination for the prevention and treatment of diseases caused by infection with Teurella multocida.

That is, as described below, the above-mentioned specific types of herbal medicines and their extracts are effective against bacterial P in vitro and in vivo.
It was revealed that it has bacteriostatic and bactericidal effects specifically against a5teurella multocida. And this Pa5Leurella mulLoci
As a result of the inhibitory effect on da, when the above-mentioned herbal medicines were administered to pigs,
It was possible to prevent and cure pasteurellosis, a disease of pigs caused by CLD, a.

The effectiveness of the above-mentioned specific herbal medicines and their extracts against this disease unique to pigs was unexpected and was revealed for the first time by the present inventors.

These herbal medicines can be directly administered in the form of raw powder, extracts, or active ingredients of herbal medicines, or they can be added to feed, or they can be administered as solution preparations, dispersion preparations, semi-solid preparations, powder preparations, molded preparations, etc. It is used in formulations such as infusion preparations and injection preparations.

The extract is used, for example, by solvent extraction using water, an organic solvent, or a mixture of water and an organic solvent, and used as it is, concentrated, diluted, or after removing the solvent.

Examples of organic solvents include methanol, ethanol, n
-Propertool, n-butanol, acetone, ethyl acetate, ether, methylene chloride, chloroform, benzene, carbon tetrachloride, petroleum ether, etc. are used, and methanol and ethanol are particularly preferred. These organic solvents can be used alone or as a mixture of two or more.

This solvent extraction can be carried out by cold soaking or digesting the herbal medicine in these solvents. 15-25℃ for cold soaking
Digestion can be carried out at a temperature range of 35-45°C. The extraction time will vary depending on the extraction temperature, but may be approximately 5 days or until the soluble components are fully dissolved in the shell.

The active ingredients of herbal medicines can be obtained by, for example, shaking the above-mentioned extract using various solvents, collecting the fraction that migrates to the solvent, and distilling off the solvent, which can be used as the effective fraction, that is, the active ingredient. can.

Solvents used in this case include nonpolar solvents such as lower aliphatic ethers (diethyl ether, ethyl methyl ether, etc.); lower halogen alkanes (chloroform, ethyl methyl ether, etc.);
Examples include dichloroethane, dibromoethane, etc.); benzenes (benzene, toluene, xylene, etc.); and other petroleum solvents (petroleum ether, petroleum benzene, ligroin, etc.).

In addition, the remaining aqueous solution from which this effective fraction was extracted is extracted by shaking using water and a kind of organic solvent saturated with water, washed with water, and transferred to the organic solvent layer that is the residue. A fraction obtained by distilling off the solvent can also be used as an effective fraction, that is, an active ingredient. This effective fraction has different physicochemical properties and different detected components from the above-mentioned effective fraction.

The dose is 0.001 gg/calculated as the weight of the raw drug powder.
Body weight kg/day ranges from 100K/kg body weight/day.

This amount will be different for use as a prophylactic agent and as a therapeutic agent - the dose in the shell will be higher in the latter case.

The administration method can be oral or parenteral administration, and examples of parenteral administration include intramuscular administration, intraperitoneal administration, transdermal administration, nasal administration, intravenous administration, and the like.

[Effects of the Invention] As detailed above, the present invention provides an effective preventive and therapeutic agent and an effective preventive and therapeutic method for pasteurellosis in pigs.

Since the prophylactic and therapeutic agent of the present invention uses herbal medicines or extracts obtained from them as herbal medicines, there is no problem of the development of resistant bacteria or side effects associated with antibiotics, etc., and it can be administered with confidence.

In addition, since there is no concern about the effects of residual drug on the human body, it is suitable as a drug for the prevention and treatment of Pasteurella in pigs.

Furthermore, the disease can be prevented and treated at a lower cost than when using antibiotics or the like.

The present invention will be explained in more detail below using test examples and examples.

Test Example 1 The sensitivity of Pa5teurella llultoclda to various crude drugs was investigated.

Various crude drugs were added to a 50% aqueous ethanol solution in an amount seven times the amount of the crude drugs, and extracted for 16 hours to obtain alcoholic extracts.

Moreover, 7 to 15 times the amount of water was used in place of the 50% ethanol aqueous solution, and this was extracted in a hot bath at 100° C. for 1 hour to obtain a water extract.

The extract was infiltrated into an 8-diameter disk in an amount of 25 μi each and dried. This disc is Pa5teurcl Ia
The plate was placed on a blood agar medium coated with a strain of IIultocida and incubated at 37°C for 18 hours, after which the diameter of the inhibition zone was measured.

Next, the test results are shown in Tables 1 and 2.

Table 1 18
Oh Shin 27
Oubari 19 Ogon 13 Gennosyouko 11 Kanzo 9 Enmeiso 15 Chu 12 Ho
Table 1 (Continued) Gobaishi 17
Tanjin IO
Koouren IO aroe 14
Cho Ji 16
Bye bye 1
5 Table 2 Herbal medicine name Inhibition circle by water extract (mm) Daiou 17 Oushin 21 Obari 11 Enmeiso 11 Gyutan 14 Gobaishi 13
aroe 1
1 Example 1 Basic feed corn 250 parts by weight Milo
492
35 Defatted rice bran
35 l'soybean meal
85〃Na Tane Kasu
40 Cotton seed lees
lO〃Fish meal (60%)
5 〃Meat bone powder l
O〃Sugar dense 20〃
Calcium carbonate 11 Salt 3 Premix 4 2% by weight of Oren powder was added to the basic feed to prepare a test feed. This test feed was fed ad libitum to 80 healthy pigs aged 60 days and raised until they were 180 days old.

In the control group, basal feed was given instead of the above-mentioned test feed.

During the breeding period, the number of animals exhibiting pneumonia symptoms due to Pasteurella infection in the field and the number of animals dying from pneumonia were observed.

As a result, in the test area, 1 (0) 780 pigs exhibited pneumonia symptoms due to Pasteurella, while in the control area, the number was 20 (18) 780 pigs. The numbers in parentheses above indicate the number of animals that died from pneumonia caused by Pasteurella.

Examples 2 to 17 The same procedure as in Example 1 was conducted except that in Examples 2 to 17, the herbal medicine shown below was used instead of Oren.

Next, the test results are shown in the table below.

(Left below) Pasteurella 2 Daio cormorant 1 head (0) / 80 heads 20 heads (18)
/ 80 heads 3 Over 2 heads (1) / 80 heads 2
0 head (18) /80 head 4 Ougon 2 head (1
) / 80 heads 20 heads (18) / 80 heads 5 Genno Shoko 2 heads (2) 780 heads 20 heads (18) / 80 heads 6 Kan Elephant 3 heads (3) / 80 heads 20 heads (18)
/ 80 heads 7 Enmeiso 3 heads (1) / 80 heads 2
0 (18) / 80 8 Chu 3 (
3) 780 20 (18) / 80 9
Up 4 (3) 780 20 (1g) /8
0 head 10 Gyutan 2 head (1) / 80 head 20 head (1
B) /80 heads 11 Cornelius 3 heads (3) /80 heads 20 heads (18) /80 heads 12 Gobaishi 3 heads (2) /80 heads 20 heads (18) /80 heads 13 Tan Jin 4 heads (4)/81) 20 (18)
/ 80 heads 14 Nio 9123 heads (3) / 80 heads 20
Head (18) / 80 heads 15 7 Roe 1 head (
1) 780 20 (18) /80 16
2 (2) 780 20 (1B) /
80 17 U Bai 2 (2) 780 20 (18) 780 The numbers in parentheses in the table above indicate the number of animals that died from pneumonia caused by Pasteurella.

Example 18 Rhubarb, orientalis, orientalis, orientalis, gennoshoko, licorice, daylily, chiyu, hop, gyutan, cornelian, gobaishi, tanjin, orensis,
1 kg of each powder of aloe, clove, and ubai was taken, and each powder was added to 10 II of water and extracted at 100° C. for 1 hour. Each water extract was diluted 1000 times to prepare a test extract. 60 each for both test and control areas.
Thirty day-old pigs were used, and the control group was given water, and the test group was given the test extract instead of water. The same basal feed as that used in Example 1 was used for both the control and test plots. The animals were raised in this manner until they were 180 days old, and the number of animals that developed pneumonia due to field-infected Pasteurella and the number of animals that died during that period was observed. As a result, the number of animals that developed pneumonia and died due to field infection with Pasteurella was lower in all test plots than in the control plots.

Claims (1)

[Scope of Claims] 1) One species selected from the group consisting of rhubarb, orientalis, orientalis, scutellariae, scutellariae, licorice, daylily, chiyu, hop, gyutan, cornelian, burr, chinsugar, trumpet, aloe, clove, and urba. Or a preventive and therapeutic agent for pasteurellosis in pigs containing two or more kinds of herbal medicines. 2) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is in the form of bulk powder. 3) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is an organic solvent, a mixture of water and an organic solvent, a crude drug extract extracted with water, and/or a crude drug active ingredient obtained therefrom. 4) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is blended into the feed. 5) One or more herbal medicines selected from the group consisting of rhubarb, orientalis, orientalis, scutellariae, scutellariae, licorice, daylily, chiyu, hop, gyutan, cornshuyu, gobaishi, tanjin, orientalis, aloe, clove, and ubai. A method for preventing and treating pasteurellosis in pigs, comprising administering an effective amount of. 6) Claim 5 in which the crude drug is used in the form of bulk powder
Prevention and treatment methods described in Section. 7) The prevention and treatment method according to claim 5, wherein the herbal medicine is an organic solvent, a mixture of water and an organic solvent, a herbal medicine extract extracted with water, and/or an active herbal medicine ingredient obtained therefrom. 8) The prevention and treatment method according to claim 5, wherein the herbal medicine is mixed with feed and administered.