JP7848199B2 - マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法 - Google Patents

マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法

Info

Publication number
JP7848199B2
JP7848199B2 JP2023526474A JP2023526474A JP7848199B2 JP 7848199 B2 JP7848199 B2 JP 7848199B2 JP 2023526474 A JP2023526474 A JP 2023526474A JP 2023526474 A JP2023526474 A JP 2023526474A JP 7848199 B2 JP7848199 B2 JP 7848199B2
Authority
JP
Japan
Prior art keywords
kif11
target
nucleic acid
amplification
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023526474A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023547498A (ja
JP2023547498A5 (https=
Inventor
デヴォゲレア ブノワ
クラース バート
ピオフチク トーマス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocartis NV
Original Assignee
Biocartis NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocartis NV filed Critical Biocartis NV
Publication of JP2023547498A publication Critical patent/JP2023547498A/ja
Publication of JP2023547498A5 publication Critical patent/JP2023547498A5/ja
Application granted granted Critical
Publication of JP7848199B2 publication Critical patent/JP7848199B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30Oligonucleotides characterised by their secondary structure
    • C12Q2525/301Hairpin oligonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125Rolling circle
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00Nucleic acid detection characterised by assay method
    • C12Q2561/109Invader technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00Nucleic acid detection characterised by assay method
    • C12Q2561/113Real time assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/629Detection means characterised by use of a special device being a microfluidic device
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
JP2023526474A 2020-10-29 2021-10-29 マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法 Active JP7848199B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP20204806 2020-10-29
EP20204806.2 2020-10-29
EP20214901 2020-12-17
EP20214901.9 2020-12-17
PCT/EP2021/080221 WO2022090521A1 (en) 2020-10-29 2021-10-29 Generic cartridge and method for multiplex nucleic acid detection

Publications (3)

Publication Number Publication Date
JP2023547498A JP2023547498A (ja) 2023-11-10
JP2023547498A5 JP2023547498A5 (https=) 2024-11-06
JP7848199B2 true JP7848199B2 (ja) 2026-04-20

Family

ID=78536187

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023526474A Active JP7848199B2 (ja) 2020-10-29 2021-10-29 マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法

Country Status (8)

Country Link
US (1) US20240035075A1 (https=)
EP (2) EP3992302A1 (https=)
JP (1) JP7848199B2 (https=)
KR (1) KR20230098259A (https=)
AU (1) AU2021367997A1 (https=)
CA (1) CA3200315A1 (https=)
ES (1) ES3034761T3 (https=)
WO (1) WO2022090521A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023067110A1 (en) * 2021-10-20 2023-04-27 Tataa Biocenter Ab Methods and compositions for detection of mutant nucleic acid sequences
WO2024240872A1 (en) 2023-05-23 2024-11-28 Biocartis Nv Cartridges comprising tablets
WO2025141779A1 (ja) * 2023-12-27 2025-07-03 株式会社日立ハイテク 血漿の分取条件の決定方法、血漿分取装置、それを備えたdna抽出装置
WO2025141758A1 (ja) * 2023-12-27 2025-07-03 株式会社日立ハイテク 検体検査方法及び検体検査システム

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014533107A (ja) 2011-11-10 2014-12-11 アルベルト−ルートヴィヒ−ウニベルシタット フライブルク 普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ
WO2018190365A1 (ja) 2017-04-12 2018-10-18 国立大学法人九州大学 神経障害性疼痛マーカー及びその使用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2689172T3 (es) 2005-06-23 2018-11-08 Biocartis Nv Cartucho modular, sistema y procedimiento para diagnóstico médico automatizado
WO2007004103A1 (en) 2005-06-30 2007-01-11 Koninklijke Philips Electronics N.V. Cartridge for automated medical diagnostics
JP5665272B2 (ja) 2005-10-07 2015-02-04 スピーデクス ピーティーワイ リミテッド 多成分核酸酵素およびそれらの使用方法
US8598333B2 (en) * 2006-05-26 2013-12-03 Alnylam Pharmaceuticals, Inc. SiRNA silencing of genes expressed in cancer
EP2310522B1 (en) * 2008-06-17 2013-03-20 Sigma-Aldrich Co. LLC Real time polymerase chain reaction process using a universal detection system
EP2419705B1 (en) 2009-04-14 2020-12-09 Biocartis NV Treatment of a sample with focused acoustic energy
US9587270B2 (en) * 2009-06-29 2017-03-07 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
CN103917664B (zh) 2011-09-09 2018-06-15 斯比戴克斯私人有限公司 核酸酶底物
CA2863808C (en) 2012-02-20 2020-07-21 Speedx Pty Ltd Methods and oligonucleotides for amplifying and/or detecting nucleic acids
WO2014128129A1 (en) 2013-02-25 2014-08-28 Biocartis N.V. Isolation of nucleic acids
CN114471756B (zh) * 2013-11-18 2024-04-16 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
EP3325152B1 (en) * 2015-07-23 2022-12-07 Biocartis NV Automated sample to ngs library preparation
WO2017013102A1 (en) * 2015-07-23 2017-01-26 Biocartis Nv Optimized clinical sample sequencing
KR101961642B1 (ko) * 2016-04-25 2019-03-25 (주)진매트릭스 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물
CN108823287B (zh) * 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
US20210189473A1 (en) * 2018-09-06 2021-06-24 ChromaCode,Inc. Universal tail primers with multiple binding motifs for multiplexed detection of single nucleotide polymorphisms
US20220195510A1 (en) 2019-02-12 2022-06-23 Biocartis Nv Protocols and kits for multiplex amplification and ngs-specific tagging

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014533107A (ja) 2011-11-10 2014-12-11 アルベルト−ルートヴィヒ−ウニベルシタット フライブルク 普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ
WO2018190365A1 (ja) 2017-04-12 2018-10-18 国立大学法人九州大学 神経障害性疼痛マーカー及びその使用

Also Published As

Publication number Publication date
ES3034761T3 (en) 2025-08-22
EP3992302A1 (en) 2022-05-04
JP2023547498A (ja) 2023-11-10
EP4237584A1 (en) 2023-09-06
US20240035075A1 (en) 2024-02-01
EP4237584C0 (en) 2025-04-23
WO2022090521A1 (en) 2022-05-05
EP4237584B1 (en) 2025-04-23
KR20230098259A (ko) 2023-07-03
CA3200315A1 (en) 2022-05-05
AU2021367997A1 (en) 2023-06-08

Similar Documents

Publication Publication Date Title
JP7848199B2 (ja) マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法
US20240301483A1 (en) Dna mutation detection employing enrichment of mutant polynucleotide sequences and minimally invasive sampling
US20210292811A1 (en) Multiplexed assay for quantitating and assessing integrity of cell-free dna in biological fluids for cancer diagnosis, prognosis and surveillance
US9862995B2 (en) Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
JP2019162102A (ja) 末梢血中で、がんによって変化したrnaを検出するシステムおよび方法
CN109996860A (zh) 用于核酸制备的系统
Beikircher et al. Multiplexed and sensitive DNA methylation testing using methylation-sensitive restriction enzymes “MSRE-qPCR”
CN108699553A (zh) 用于筛选甲状腺癌中突变的组合物和方法
JPWO2007083766A1 (ja) 分子内プローブによる核酸配列の検出方法
Wainman et al. Applications of digital polymerase chain reaction (dPCR) in molecular and clinical testing
CN105368932B (zh) 基于焦磷酸测序技术的结核分枝杆菌(tb)耐药性基因的检测技术
Sho et al. Precision oncology using a limited number of cells: Optimization of whole genome amplification products for sequencing applications
WO2014145298A1 (en) Nucleic acid amplification
Ruiz et al. Single‐molecule detection of cancer mutations using a novel PCR‐LDR‐qPCR assay
US20230002807A1 (en) Methods and compositions for nucleic acid analysis
Gray et al. Ultra-sensitive molecular detection of gene fusions from RNA using ASPYRE
CN116783308A (zh) 用于多重核酸检测的通用卡盒和方法
JP2025519243A (ja) 標的核酸の検出
WO2023056300A1 (en) Personalized cancer liquid biopsies using primers from a primer bank
Al-Turkmani et al. Molecular assessment of human diseases in the clinical laboratory
HK40073668A (en) Generic cartridge and method for multiplex nucleic acid detection
Deharvengt et al. Molecular assessment of human diseases in the clinical laboratory
US20250290124A1 (en) Ultra rapid droplet digital polymerase chain reaction
Gaina Introductory Chapter: Molecular Diagnostics in the Era of Precision
CN119709972A (zh) 一种单碱基突变检测方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230502

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A525

Effective date: 20230628

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241028

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20241028

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20250924

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20251006

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20251226

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20260310

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20260408

R150 Certificate of patent or registration of utility model

Ref document number: 7848199

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150