JP7848199B2 - マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法 - Google Patents
マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法Info
- Publication number
- JP7848199B2 JP7848199B2 JP2023526474A JP2023526474A JP7848199B2 JP 7848199 B2 JP7848199 B2 JP 7848199B2 JP 2023526474 A JP2023526474 A JP 2023526474A JP 2023526474 A JP2023526474 A JP 2023526474A JP 7848199 B2 JP7848199 B2 JP 7848199B2
- Authority
- JP
- Japan
- Prior art keywords
- kif11
- target
- nucleic acid
- amplification
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/109—Invader technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20204806 | 2020-10-29 | ||
| EP20204806.2 | 2020-10-29 | ||
| EP20214901 | 2020-12-17 | ||
| EP20214901.9 | 2020-12-17 | ||
| PCT/EP2021/080221 WO2022090521A1 (en) | 2020-10-29 | 2021-10-29 | Generic cartridge and method for multiplex nucleic acid detection |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023547498A JP2023547498A (ja) | 2023-11-10 |
| JP2023547498A5 JP2023547498A5 (https=) | 2024-11-06 |
| JP7848199B2 true JP7848199B2 (ja) | 2026-04-20 |
Family
ID=78536187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023526474A Active JP7848199B2 (ja) | 2020-10-29 | 2021-10-29 | マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240035075A1 (https=) |
| EP (2) | EP3992302A1 (https=) |
| JP (1) | JP7848199B2 (https=) |
| KR (1) | KR20230098259A (https=) |
| AU (1) | AU2021367997A1 (https=) |
| CA (1) | CA3200315A1 (https=) |
| ES (1) | ES3034761T3 (https=) |
| WO (1) | WO2022090521A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023067110A1 (en) * | 2021-10-20 | 2023-04-27 | Tataa Biocenter Ab | Methods and compositions for detection of mutant nucleic acid sequences |
| WO2024240872A1 (en) | 2023-05-23 | 2024-11-28 | Biocartis Nv | Cartridges comprising tablets |
| WO2025141779A1 (ja) * | 2023-12-27 | 2025-07-03 | 株式会社日立ハイテク | 血漿の分取条件の決定方法、血漿分取装置、それを備えたdna抽出装置 |
| WO2025141758A1 (ja) * | 2023-12-27 | 2025-07-03 | 株式会社日立ハイテク | 検体検査方法及び検体検査システム |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014533107A (ja) | 2011-11-10 | 2014-12-11 | アルベルト−ルートヴィヒ−ウニベルシタット フライブルク | 普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ |
| WO2018190365A1 (ja) | 2017-04-12 | 2018-10-18 | 国立大学法人九州大学 | 神経障害性疼痛マーカー及びその使用 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2689172T3 (es) | 2005-06-23 | 2018-11-08 | Biocartis Nv | Cartucho modular, sistema y procedimiento para diagnóstico médico automatizado |
| WO2007004103A1 (en) | 2005-06-30 | 2007-01-11 | Koninklijke Philips Electronics N.V. | Cartridge for automated medical diagnostics |
| JP5665272B2 (ja) | 2005-10-07 | 2015-02-04 | スピーデクス ピーティーワイ リミテッド | 多成分核酸酵素およびそれらの使用方法 |
| US8598333B2 (en) * | 2006-05-26 | 2013-12-03 | Alnylam Pharmaceuticals, Inc. | SiRNA silencing of genes expressed in cancer |
| EP2310522B1 (en) * | 2008-06-17 | 2013-03-20 | Sigma-Aldrich Co. LLC | Real time polymerase chain reaction process using a universal detection system |
| EP2419705B1 (en) | 2009-04-14 | 2020-12-09 | Biocartis NV | Treatment of a sample with focused acoustic energy |
| US9587270B2 (en) * | 2009-06-29 | 2017-03-07 | Luminex Corporation | Chimeric primers with hairpin conformations and methods of using same |
| CN103917664B (zh) | 2011-09-09 | 2018-06-15 | 斯比戴克斯私人有限公司 | 核酸酶底物 |
| CA2863808C (en) | 2012-02-20 | 2020-07-21 | Speedx Pty Ltd | Methods and oligonucleotides for amplifying and/or detecting nucleic acids |
| WO2014128129A1 (en) | 2013-02-25 | 2014-08-28 | Biocartis N.V. | Isolation of nucleic acids |
| CN114471756B (zh) * | 2013-11-18 | 2024-04-16 | 尹特根埃克斯有限公司 | 用于样本分析的卡盒和仪器 |
| EP3325152B1 (en) * | 2015-07-23 | 2022-12-07 | Biocartis NV | Automated sample to ngs library preparation |
| WO2017013102A1 (en) * | 2015-07-23 | 2017-01-26 | Biocartis Nv | Optimized clinical sample sequencing |
| KR101961642B1 (ko) * | 2016-04-25 | 2019-03-25 | (주)진매트릭스 | 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물 |
| CN108823287B (zh) * | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
| US20210189473A1 (en) * | 2018-09-06 | 2021-06-24 | ChromaCode,Inc. | Universal tail primers with multiple binding motifs for multiplexed detection of single nucleotide polymorphisms |
| US20220195510A1 (en) | 2019-02-12 | 2022-06-23 | Biocartis Nv | Protocols and kits for multiplex amplification and ngs-specific tagging |
-
2021
- 2021-10-29 KR KR1020237017929A patent/KR20230098259A/ko active Pending
- 2021-10-29 JP JP2023526474A patent/JP7848199B2/ja active Active
- 2021-10-29 US US18/250,555 patent/US20240035075A1/en active Pending
- 2021-10-29 WO PCT/EP2021/080221 patent/WO2022090521A1/en not_active Ceased
- 2021-10-29 CA CA3200315A patent/CA3200315A1/en active Pending
- 2021-10-29 ES ES21805437T patent/ES3034761T3/es active Active
- 2021-10-29 EP EP21205713.7A patent/EP3992302A1/en active Pending
- 2021-10-29 AU AU2021367997A patent/AU2021367997A1/en active Pending
- 2021-10-29 EP EP21805437.7A patent/EP4237584B1/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014533107A (ja) | 2011-11-10 | 2014-12-11 | アルベルト−ルートヴィヒ−ウニベルシタット フライブルク | 普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ |
| WO2018190365A1 (ja) | 2017-04-12 | 2018-10-18 | 国立大学法人九州大学 | 神経障害性疼痛マーカー及びその使用 |
Also Published As
| Publication number | Publication date |
|---|---|
| ES3034761T3 (en) | 2025-08-22 |
| EP3992302A1 (en) | 2022-05-04 |
| JP2023547498A (ja) | 2023-11-10 |
| EP4237584A1 (en) | 2023-09-06 |
| US20240035075A1 (en) | 2024-02-01 |
| EP4237584C0 (en) | 2025-04-23 |
| WO2022090521A1 (en) | 2022-05-05 |
| EP4237584B1 (en) | 2025-04-23 |
| KR20230098259A (ko) | 2023-07-03 |
| CA3200315A1 (en) | 2022-05-05 |
| AU2021367997A1 (en) | 2023-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7848199B2 (ja) | マルチプレックス核酸検出のためのジェネリックカートリッジおよび方法 | |
| US20240301483A1 (en) | Dna mutation detection employing enrichment of mutant polynucleotide sequences and minimally invasive sampling | |
| US20210292811A1 (en) | Multiplexed assay for quantitating and assessing integrity of cell-free dna in biological fluids for cancer diagnosis, prognosis and surveillance | |
| US9862995B2 (en) | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing | |
| JP2019162102A (ja) | 末梢血中で、がんによって変化したrnaを検出するシステムおよび方法 | |
| CN109996860A (zh) | 用于核酸制备的系统 | |
| Beikircher et al. | Multiplexed and sensitive DNA methylation testing using methylation-sensitive restriction enzymes “MSRE-qPCR” | |
| CN108699553A (zh) | 用于筛选甲状腺癌中突变的组合物和方法 | |
| JPWO2007083766A1 (ja) | 分子内プローブによる核酸配列の検出方法 | |
| Wainman et al. | Applications of digital polymerase chain reaction (dPCR) in molecular and clinical testing | |
| CN105368932B (zh) | 基于焦磷酸测序技术的结核分枝杆菌(tb)耐药性基因的检测技术 | |
| Sho et al. | Precision oncology using a limited number of cells: Optimization of whole genome amplification products for sequencing applications | |
| WO2014145298A1 (en) | Nucleic acid amplification | |
| Ruiz et al. | Single‐molecule detection of cancer mutations using a novel PCR‐LDR‐qPCR assay | |
| US20230002807A1 (en) | Methods and compositions for nucleic acid analysis | |
| Gray et al. | Ultra-sensitive molecular detection of gene fusions from RNA using ASPYRE | |
| CN116783308A (zh) | 用于多重核酸检测的通用卡盒和方法 | |
| JP2025519243A (ja) | 標的核酸の検出 | |
| WO2023056300A1 (en) | Personalized cancer liquid biopsies using primers from a primer bank | |
| Al-Turkmani et al. | Molecular assessment of human diseases in the clinical laboratory | |
| HK40073668A (en) | Generic cartridge and method for multiplex nucleic acid detection | |
| Deharvengt et al. | Molecular assessment of human diseases in the clinical laboratory | |
| US20250290124A1 (en) | Ultra rapid droplet digital polymerase chain reaction | |
| Gaina | Introductory Chapter: Molecular Diagnostics in the Era of Precision | |
| CN119709972A (zh) | 一种单碱基突变检测方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230502 |
|
| A524 | Written submission of copy of amendment under article 19 pct |
Free format text: JAPANESE INTERMEDIATE CODE: A525 Effective date: 20230628 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20241028 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20241028 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20250924 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20251006 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20251226 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20260310 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20260408 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 7848199 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |