JP7784060B2 - ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法 - Google Patents

３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Info

Publication number
JP7784060B2
JP7784060B2 JP2021515234A JP2021515234A JP7784060B2 JP 7784060 B2 JP7784060 B2 JP 7784060B2 JP 2021515234 A JP2021515234 A JP 2021515234A JP 2021515234 A JP2021515234 A JP 2021515234A JP 7784060 B2 JP7784060 B2 JP 7784060B2
Authority
JP
Japan
Prior art keywords
acid
coa
gene
microorganism
strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021515234A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2021187533A5 (https=
JPWO2021187533A1 (https=
Inventor
匡平 磯部
健司 河村
勝成 山田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Publication of JPWO2021187533A1 publication Critical patent/JPWO2021187533A1/ja
Publication of JPWO2021187533A5 publication Critical patent/JPWO2021187533A5/ja
Application granted granted Critical
Publication of JP7784060B2 publication Critical patent/JP7784060B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/425—Serratia

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021515234A 2020-03-18 2021-03-17 ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法 Active JP7784060B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020047328 2020-03-18
JP2020047328 2020-03-18
PCT/JP2021/010871 WO2021187533A1 (ja) 2020-03-18 2021-03-17 ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Publications (3)

Publication Number Publication Date
JPWO2021187533A1 JPWO2021187533A1 (https=) 2021-09-23
JPWO2021187533A5 JPWO2021187533A5 (https=) 2024-03-04
JP7784060B2 true JP7784060B2 (ja) 2025-12-11

Family

ID=77772113

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021515234A Active JP7784060B2 (ja) 2020-03-18 2021-03-17 ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Country Status (4)

Country Link
US (1) US12577592B2 (https=)
JP (1) JP7784060B2 (https=)
CN (1) CN115279890A (https=)
WO (1) WO2021187533A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12275979B2 (en) * 2017-11-30 2025-04-15 Toray Industries, Inc. Gene-modified microorganism for producing 3-hydroxyadipic acid, alpha-hydromuconic acid, and/or adipic acid, and production method for said chemical products
WO2023182322A1 (ja) 2022-03-23 2023-09-28 東レ株式会社 ３－ヒドロキシアジピン酸および／または３－オキソアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
WO2025063258A1 (ja) * 2023-09-21 2025-03-27 東レ株式会社 α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015005406A1 (ja) 2013-07-09 2015-01-15 味の素株式会社 有用物質の製造方法
WO2019107516A1 (ja) 2017-11-30 2019-06-06 東レ株式会社 ３－ヒドロキシアジピン酸、α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3309258B1 (en) * 2015-06-10 2020-06-03 Toray Industries, Inc. Method for producing 3-hydroxyadipic acid
JP6999090B2 (ja) * 2016-05-31 2022-01-18 東レ株式会社 ３－ヒドロキシアジピン酸の製造方法
JP6948595B2 (ja) * 2016-05-31 2021-10-13 東レ株式会社 α−ヒドロムコン酸の製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015005406A1 (ja) 2013-07-09 2015-01-15 味の素株式会社 有用物質の製造方法
WO2019107516A1 (ja) 2017-11-30 2019-06-06 東レ株式会社 ３－ヒドロキシアジピン酸、α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Also Published As

Publication number Publication date
US12577592B2 (en) 2026-03-17
JPWO2021187533A1 (https=) 2021-09-23
US20230101086A1 (en) 2023-03-30
WO2021187533A1 (ja) 2021-09-23
CN115279890A (zh) 2022-11-01

Similar Documents

Publication Publication Date Title
JP7331691B2 (ja) ３－ヒドロキシアジピン酸、α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP7530554B2 (ja) ３－ヒドロキシアジピン酸、α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP7784060B2 (ja) ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP6999090B2 (ja) ３－ヒドロキシアジピン酸の製造方法
JP7646119B2 (ja) ３－ヒドロキシアジピン酸、α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
EP3467113B1 (en) Method for producing a-hydromuconic acid
CN116406420A (zh) 用于生产3-羟基己二酸和/或α-氢化己二烯二酸的基因修饰微生物以及该化学品的制造方法
EP4497819A1 (en) Genetically-modified microorganism for producing 3-hydroxyadipic acid and/or 3-oxoadipic acid and production method for said chemical products
WO2023157816A1 (ja) ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP7769868B2 (ja) 遺伝子改変微生物および有機酸の製造方法
JP2024090463A (ja) ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
WO2025063258A1 (ja) α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
US20250283030A1 (en) Biosynthesis of Phenylpropanoid Compounds
JP2023144366A (ja) ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
WO2022181654A1 (ja) 変異型アシル－ＣｏＡヒドロラーゼ
WO2024242124A1 (ja) ３－ヒドロキシアジピン酸、α－ヒドロムコン酸および／またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240222

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240222

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250123

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250321

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250618

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250729

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20251031

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20251113

R150 Certificate of patent or registration of utility model

Ref document number: 7784060

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150