JP7781686B2 - バイオセンサを用いた酸化還元酵素の電気化学的測定方法及びそれに用いるバイオセンサ - Google Patents

バイオセンサを用いた酸化還元酵素の電気化学的測定方法及びそれに用いるバイオセンサ

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Publication number
JP7781686B2
JP7781686B2 JP2022042572A JP2022042572A JP7781686B2 JP 7781686 B2 JP7781686 B2 JP 7781686B2 JP 2022042572 A JP2022042572 A JP 2022042572A JP 2022042572 A JP2022042572 A JP 2022042572A JP 7781686 B2 JP7781686 B2 JP 7781686B2
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JP
Japan
Prior art keywords
mediator
oxidoreductase
reagent layer
substrate
biosensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022042572A
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English (en)
Japanese (ja)
Other versions
JP2023136725A5 (enExample
JP2023136725A (ja
Inventor
耕太郎 篠嵜
美咲 大賀
寿幸 三上
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arkray Inc
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Priority to JP2022042572A priority Critical patent/JP7781686B2/ja
Priority to EP23162446.1A priority patent/EP4245857A1/en
Priority to US18/185,305 priority patent/US20230293059A1/en
Priority to CN202310265289.9A priority patent/CN116773620A/zh
Publication of JP2023136725A publication Critical patent/JP2023136725A/ja
Publication of JP2023136725A5 publication Critical patent/JP2023136725A5/ja
Application granted granted Critical
Publication of JP7781686B2 publication Critical patent/JP7781686B2/ja
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue for measuring glucose, e.g. by tissue impedance measurement
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/1468—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using chemical or electrochemical methods, e.g. by polarographic means
    • A61B5/1486—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using chemical or electrochemical methods, e.g. by polarographic means using enzyme electrodes, e.g. with immobilised oxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Emergency Medicine (AREA)
  • Optics & Photonics (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022042572A 2022-03-17 2022-03-17 バイオセンサを用いた酸化還元酵素の電気化学的測定方法及びそれに用いるバイオセンサ Active JP7781686B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2022042572A JP7781686B2 (ja) 2022-03-17 2022-03-17 バイオセンサを用いた酸化還元酵素の電気化学的測定方法及びそれに用いるバイオセンサ
EP23162446.1A EP4245857A1 (en) 2022-03-17 2023-03-16 Method for electrochemically measuring oxidoreductase using a biosensor, and biosensor used therein
US18/185,305 US20230293059A1 (en) 2022-03-17 2023-03-16 Method for electrochemically measuring oxidoreductase using a biosensor, and biosensor used therein
CN202310265289.9A CN116773620A (zh) 2022-03-17 2023-03-16 使用生物传感器的氧化还原酶的电化学测定方法以及其中使用的生物传感器

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2022042572A JP7781686B2 (ja) 2022-03-17 2022-03-17 バイオセンサを用いた酸化還元酵素の電気化学的測定方法及びそれに用いるバイオセンサ

Publications (3)

Publication Number Publication Date
JP2023136725A JP2023136725A (ja) 2023-09-29
JP2023136725A5 JP2023136725A5 (enExample) 2025-01-15
JP7781686B2 true JP7781686B2 (ja) 2025-12-08

Family

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JP2022042572A Active JP7781686B2 (ja) 2022-03-17 2022-03-17 バイオセンサを用いた酸化還元酵素の電気化学的測定方法及びそれに用いるバイオセンサ

Country Status (4)

Country Link
US (1) US20230293059A1 (enExample)
EP (1) EP4245857A1 (enExample)
JP (1) JP7781686B2 (enExample)
CN (1) CN116773620A (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117030820B (zh) * 2023-09-28 2024-01-09 艾康生物技术(杭州)有限公司 一种生物传感器的测量方法
JP7638430B1 (ja) * 2024-07-30 2025-03-03 キッコーマン株式会社 試薬組成物及びセンサ並びにその製造方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010507808A (ja) 2006-10-24 2010-03-11 バイエル・ヘルスケア・エルエルシー 過渡減衰電流測定法
JP2015517302A (ja) 2012-05-02 2015-06-22 アイセンス，インコーポレーテッド 酸化還元反応用試薬組成物
JP2019521343A (ja) 2016-07-12 2019-07-25 アセンシア・ダイアベティス・ケア・ホールディングス・アーゲーＡｓｃｅｎｓｉａ Ｄｉａｂｅｔｅｓ Ｃａｒｅ Ｈｏｌｄｉｎｇｓ ＡＧ ２つの電極からの交替する出力信号の使用による電気化学分析のための方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU644059B2 (en) 1988-03-15 1993-12-02 Roche Diagnostics Corporation Method and apparatus for amperometric diagnostic analysis
US5243516A (en) 1989-12-15 1993-09-07 Boehringer Mannheim Corporation Biosensing instrument and method
DE4003194A1 (de) * 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Verfahren und sensorelektrodensystem zur elektrochemischen bestimmung eines analyts oder einer oxidoreduktase sowie verwendung hierfuer geeigneter verbindungen
JPH07101215B2 (ja) 1990-04-11 1995-11-01 国立身体障害者リハビリテーションセンター総長 生体機能物質固定化電極を用いた分析法
US5366609A (en) 1993-06-08 1994-11-22 Boehringer Mannheim Corporation Biosensing meter with pluggable memory key
AUPN363995A0 (en) 1995-06-19 1995-07-13 Memtec Limited Electrochemical cell
WO2005043146A1 (ja) 2003-10-30 2005-05-12 Arkray, Inc. バイオセンサおよびその製造方法
JP2007225305A (ja) * 2006-02-21 2007-09-06 Denso Corp 酵素電極
JP5584740B2 (ja) 2011-09-26 2014-09-03 アークレイ株式会社 グルコースセンサ
JP6713364B2 (ja) 2016-07-20 2020-06-24 アークレイ株式会社 アスコルビン酸応答電極およびバイオセンサ
KR102372113B1 (ko) * 2016-10-05 2022-03-07 에프. 호프만-라 로슈 아게 다중 분석물 진단 테스트 엘리먼트들을 위한 검출 시약들 및 전극 배열들, 그리고 그것을 사용하는 방법들

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010507808A (ja) 2006-10-24 2010-03-11 バイエル・ヘルスケア・エルエルシー 過渡減衰電流測定法
JP2015517302A (ja) 2012-05-02 2015-06-22 アイセンス，インコーポレーテッド 酸化還元反応用試薬組成物
JP2019521343A (ja) 2016-07-12 2019-07-25 アセンシア・ダイアベティス・ケア・ホールディングス・アーゲーＡｓｃｅｎｓｉａ Ｄｉａｂｅｔｅｓ Ｃａｒｅ Ｈｏｌｄｉｎｇｓ ＡＧ ２つの電極からの交替する出力信号の使用による電気化学分析のための方法

Also Published As

Publication number Publication date
EP4245857A1 (en) 2023-09-20
CN116773620A (zh) 2023-09-19
US20230293059A1 (en) 2023-09-21
JP2023136725A (ja) 2023-09-29

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