JP7734442B2 - ウエルアレイを用いたウイルス検出方法、ウエルアレイ及び検出装置 - Google Patents

ウエルアレイを用いたウイルス検出方法、ウエルアレイ及び検出装置

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Publication number
JP7734442B2
JP7734442B2 JP2023508905A JP2023508905A JP7734442B2 JP 7734442 B2 JP7734442 B2 JP 7734442B2 JP 2023508905 A JP2023508905 A JP 2023508905A JP 2023508905 A JP2023508905 A JP 2023508905A JP 7734442 B2 JP7734442 B2 JP 7734442B2
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wells
well
virus
detection
area
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JPWO2022202217A1 (https=
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真 藤巻
裕樹 芦葉
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National Institute of Advanced Industrial Science and Technology AIST
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National Institute of Advanced Industrial Science and Technology AIST
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/11—Orthomyxoviridae, e.g. influenza virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/10—Detection of antigens from microorganism in sample from host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023508905A 2021-03-26 2022-03-04 ウエルアレイを用いたウイルス検出方法、ウエルアレイ及び検出装置 Active JP7734442B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021053010 2021-03-26
JP2021053010 2021-03-26
PCT/JP2022/009368 WO2022202217A1 (ja) 2021-03-26 2022-03-04 ウエルアレイを用いたウイルス検出方法、ウエルアレイ及び検出装置

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JPWO2022202217A1 JPWO2022202217A1 (https=) 2022-09-29
JP7734442B2 true JP7734442B2 (ja) 2025-09-05

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US (1) US20240168023A1 (https=)
EP (1) EP4290221A4 (https=)
JP (1) JP7734442B2 (https=)
WO (1) WO2022202217A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117264749B (zh) * 2023-11-23 2024-02-02 中国科学院空天信息创新研究院 多指标检测装置及其使用方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010112777A (ja) 2008-11-05 2010-05-20 Saitama Univ ウィルス、微生物類の検出方法
JP5551798B2 (ja) 2010-03-01 2014-07-16 クワンテリクス コーポレーション ビーズまたは他の捕捉物を用いた分子または粒子の超高感度検出
WO2016006208A1 (ja) 2014-07-08 2016-01-14 国立研究開発法人科学技術振興機構 物質封入方法及びターゲット分子を検出する方法
JP2018038384A (ja) 2016-09-05 2018-03-15 国立研究開発法人科学技術振興機構 病原性微生物検出のための方法及びキット
WO2018181488A1 (ja) 2017-03-29 2018-10-04 国立研究開発法人科学技術振興機構 微小物質検出方法及び微小物質検出用デバイス

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Publication number Priority date Publication date Assignee Title
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
CN103026232B (zh) 2010-03-01 2015-02-04 匡特里克斯公司 扩大用于检测分子或颗粒的测定法中的动态范围的方法和系统
EP2685266A1 (en) 2011-03-08 2014-01-15 Japan Science And Technology Agency Bead sealing method, method for detecting target molecule, array, kit, and target molecule detection device
JP2018091737A (ja) 2016-12-05 2018-06-14 国立大学法人 東京大学 蛍光観察装置
US10324041B2 (en) * 2016-12-21 2019-06-18 Abbott Japan Co., Ltd. Optical imaging system using lateral illumination for digital assays

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010112777A (ja) 2008-11-05 2010-05-20 Saitama Univ ウィルス、微生物類の検出方法
JP5551798B2 (ja) 2010-03-01 2014-07-16 クワンテリクス コーポレーション ビーズまたは他の捕捉物を用いた分子または粒子の超高感度検出
WO2016006208A1 (ja) 2014-07-08 2016-01-14 国立研究開発法人科学技術振興機構 物質封入方法及びターゲット分子を検出する方法
JP2018038384A (ja) 2016-09-05 2018-03-15 国立研究開発法人科学技術振興機構 病原性微生物検出のための方法及びキット
WO2018181488A1 (ja) 2017-03-29 2018-10-04 国立研究開発法人科学技術振興機構 微小物質検出方法及び微小物質検出用デバイス

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LEIRS, K. et al.，Bioassay Development for Ultrasensitive Detection of Influenza A Nucleoprotein Using Digital ELISA，Analytical Chemistry，2016年08月04日，Vol.88/17，8450-8458，https://doi.org/10.1021/acs.analchem.6b00502
MINAGAWA, Yoshihiro et al.，Mobile imaging platform for digtal influenza virus counting，Lab on a Chip，2019年08月21日，Vol.19/16，2678-2687
REVZIN, Alexander ,et al.，Development of a microfabricated cytometry platform for characterization and sorting of individual leukocytes ，Lab on a Chip，2004年10月12日，vol.5, no.1，30-37，DOI: 10.1039/b405557h
TABATA,K.V. et al.，Antibody-free digital influenza virus counting based on neuraminidase activity，Scientific Reports，2019年01月31日，Vol. 9/1067，1-13，https://www.nature.com/articles/s41598-018-37994-6.pdf

Also Published As

Publication number Publication date
EP4290221A1 (en) 2023-12-13
EP4290221A4 (en) 2025-02-26
US20240168023A1 (en) 2024-05-23
JPWO2022202217A1 (https=) 2022-09-29
WO2022202217A1 (ja) 2022-09-29

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