JP7702149B2 - 効率的かつ特異的なゲノム編集のための部位特異的リコンビナーゼの融合 - Google Patents

効率的かつ特異的なゲノム編集のための部位特異的リコンビナーゼの融合 Download PDF

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JP7702149B2
JP7702149B2 JP2022533406A JP2022533406A JP7702149B2 JP 7702149 B2 JP7702149 B2 JP 7702149B2 JP 2022533406 A JP2022533406 A JP 2022533406A JP 2022533406 A JP2022533406 A JP 2022533406A JP 7702149 B2 JP7702149 B2 JP 7702149B2
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recombinase
seq
sequence
nucleic acid
site
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JP2023504826A5 (https=
JP2023504826A (ja
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ブーフホルツ フランク
ランシング フェリックス
カルピンスキー ジャネット
ロジョ ロマノス テレサ
ソンタグ ジャン
ムカメット‐ジャノバ リリヤ
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テクニスチェ ユニベルシタト ドレスデン
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Molecular Biology (AREA)
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  • Biotechnology (AREA)
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  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2022533406A 2019-12-06 2020-12-03 効率的かつ特異的なゲノム編集のための部位特異的リコンビナーゼの融合 Active JP7702149B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19214152.1A EP3831939A1 (en) 2019-12-06 2019-12-06 Fusion of site-specific recombinases for efficient and specific genome editing
EP19214152.1 2019-12-06
PCT/EP2020/084489 WO2021110846A1 (en) 2019-12-06 2020-12-03 Fusion of site-specific recombinases for efficient and specific genome editing

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JP2023504826A JP2023504826A (ja) 2023-02-07
JP2023504826A5 JP2023504826A5 (https=) 2023-07-14
JP7702149B2 true JP7702149B2 (ja) 2025-07-03

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JP2022533406A Active JP7702149B2 (ja) 2019-12-06 2020-12-03 効率的かつ特異的なゲノム編集のための部位特異的リコンビナーゼの融合

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US (1) US20250243473A1 (https=)
EP (2) EP3831939A1 (https=)
JP (1) JP7702149B2 (https=)
CN (1) CN115427558A (https=)
AU (1) AU2020396214B2 (https=)
CA (1) CA3163359A1 (https=)
MX (1) MX2022006799A (https=)
WO (1) WO2021110846A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
EP4433584A1 (en) * 2021-11-15 2024-09-25 Technische Universität Dresden Site-specific recombinases for efficient and specific genome editing
US20250145973A1 (en) * 2023-10-16 2025-05-08 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Conditioned dna modifying enzyme comprising heterologous dna binding domain
WO2026022363A1 (en) 2024-07-25 2026-01-29 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Modifying nucleic acid sequences having asymmetric target sites

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004518419A (ja) 2000-12-01 2004-06-24 ヨーロピアン モレキュラー バイオロジー ラボラトリー 基質連結型指向性進化（ＳＬｉＤＥ）
WO2018229226A1 (en) 2017-06-14 2018-12-20 Technische Universität Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
US5981830A (en) 1997-05-30 1999-11-09 Schering Aktiengesellschaft Knockout mice and their progeny with a disrupted hepsin gene
ES2256972T3 (es) * 1997-11-18 2006-07-16 Pioneer Hi-Bred International, Inc. Metodo novedoso para la integracion de un adn foraneo dentro de genomas de eucariotas.
US20060014264A1 (en) * 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
EP1942192A1 (en) 2007-01-08 2008-07-09 Heinrich-Pette-Institut für experimentelle Virologie und Immunologie Use of a tailored recombinase for the treatment of retroviral infections
EP2576798B1 (en) 2010-05-27 2016-04-27 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
CN111254500B (zh) * 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
EP2993229A1 (en) * 2014-09-02 2016-03-09 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
US10017832B2 (en) * 2015-08-25 2018-07-10 Washington University Compositions and methods for site specific recombination at asymmetric sites
KR20250133994A (ko) * 2018-05-09 2025-09-09 케로스 테라퓨틱스, 인크. 액티빈 수용체 유형 iia 변이체 및 그의 사용 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004518419A (ja) 2000-12-01 2004-06-24 ヨーロピアン モレキュラー バイオロジー ラボラトリー 基質連結型指向性進化（ＳＬｉＤＥ）
WO2018229226A1 (en) 2017-06-14 2018-12-20 Technische Universität Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
F.Buchholz, et al.，nature biotechnology，2001年，Vol.19，pp.1047-1052
K.A.Gelato, et al.，Jorunal of Molecular Biology，2008年，Vol.378, No.3，pp.653-665

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Publication number Publication date
US20250243473A1 (en) 2025-07-31
WO2021110846A1 (en) 2021-06-10
CA3163359A1 (en) 2021-06-10
AU2020396214A1 (en) 2022-06-16
MX2022006799A (es) 2022-09-07
AU2020396214B2 (en) 2025-10-30
EP4069834A1 (en) 2022-10-12
CN115427558A (zh) 2022-12-02
JP2023504826A (ja) 2023-02-07
EP3831939A1 (en) 2021-06-09

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