CN115427558A - 用于高效且特异性基因组编辑的位点-特异性重组酶的融合物 - Google Patents

用于高效且特异性基因组编辑的位点-特异性重组酶的融合物 Download PDF

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Publication number
CN115427558A
CN115427558A CN202080095838.7A CN202080095838A CN115427558A CN 115427558 A CN115427558 A CN 115427558A CN 202080095838 A CN202080095838 A CN 202080095838A CN 115427558 A CN115427558 A CN 115427558A
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Prior art keywords
recombinase
seq
sequence
site
dna
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CN202080095838.7A
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English (en)
Chinese (zh)
Inventor
F·布赫霍尔茨
F·兰辛
J·卡平斯基
T·罗霍罗曼诺斯
J·松塔格
L·穆哈梅奇亚诺娃
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Technische Universitaet Dresden
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Technische Universitaet Dresden
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Publication of CN115427558A publication Critical patent/CN115427558A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN202080095838.7A 2019-12-06 2020-12-03 用于高效且特异性基因组编辑的位点-特异性重组酶的融合物 Pending CN115427558A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19214152.1A EP3831939A1 (en) 2019-12-06 2019-12-06 Fusion of site-specific recombinases for efficient and specific genome editing
EP19214152.1 2019-12-06
PCT/EP2020/084489 WO2021110846A1 (en) 2019-12-06 2020-12-03 Fusion of site-specific recombinases for efficient and specific genome editing

Publications (1)

Publication Number Publication Date
CN115427558A true CN115427558A (zh) 2022-12-02

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CN202080095838.7A Pending CN115427558A (zh) 2019-12-06 2020-12-03 用于高效且特异性基因组编辑的位点-特异性重组酶的融合物

Country Status (8)

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US (1) US20250243473A1 (https=)
EP (2) EP3831939A1 (https=)
JP (1) JP7702149B2 (https=)
CN (1) CN115427558A (https=)
AU (1) AU2020396214B2 (https=)
CA (1) CA3163359A1 (https=)
MX (1) MX2022006799A (https=)
WO (1) WO2021110846A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
EP4433584A1 (en) * 2021-11-15 2024-09-25 Technische Universität Dresden Site-specific recombinases for efficient and specific genome editing
US20250145973A1 (en) * 2023-10-16 2025-05-08 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Conditioned dna modifying enzyme comprising heterologous dna binding domain
WO2026022363A1 (en) 2024-07-25 2026-01-29 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Modifying nucleic acid sequences having asymmetric target sites

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100172881A1 (en) * 2007-01-08 2010-07-08 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Use of tailored recombinases for the treatment of retroviral infections
WO2014093330A1 (en) * 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
WO2016034553A1 (en) * 2014-09-02 2016-03-10 Heinrich-Pette-Institut Leibniz-Institut Für Experimentelle Virologie - Stiftung Bürgerlichen Rechts Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981830A (en) 1997-05-30 1999-11-09 Schering Aktiengesellschaft Knockout mice and their progeny with a disrupted hepsin gene
ES2256972T3 (es) * 1997-11-18 2006-07-16 Pioneer Hi-Bred International, Inc. Metodo novedoso para la integracion de un adn foraneo dentro de genomas de eucariotas.
GB0029375D0 (en) * 2000-12-01 2001-01-17 Total Fina Elf Substrate linked directed evolution (slide)
US20060014264A1 (en) * 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
EP2576798B1 (en) 2010-05-27 2016-04-27 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
US10017832B2 (en) * 2015-08-25 2018-07-10 Washington University Compositions and methods for site specific recombination at asymmetric sites
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
KR20250133994A (ko) * 2018-05-09 2025-09-09 케로스 테라퓨틱스, 인크. 액티빈 수용체 유형 iia 변이체 및 그의 사용 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100172881A1 (en) * 2007-01-08 2010-07-08 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Use of tailored recombinases for the treatment of retroviral infections
WO2014093330A1 (en) * 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
WO2016034553A1 (en) * 2014-09-02 2016-03-10 Heinrich-Pette-Institut Leibniz-Institut Für Experimentelle Virologie - Stiftung Bürgerlichen Rechts Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
CN106852155A (zh) * 2014-09-02 2017-06-13 海因里希·佩特研究所莱比锡试验病毒学研究所—民法基金会 用于重组多种逆转录病毒毒株中的不对称靶位点的良好耐受且高度特异性的修整重组酶

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GELATO, K.A. ET AL: "Spatially Directed Assembly of a Heterotetrameric Cre-lox Synapse Restrics Recombination Specificity", JOURNAL OF MOLECULAR BIOLOGY, vol. 378, no. 3, pages 653 - 665 *

Also Published As

Publication number Publication date
US20250243473A1 (en) 2025-07-31
WO2021110846A1 (en) 2021-06-10
CA3163359A1 (en) 2021-06-10
AU2020396214A1 (en) 2022-06-16
MX2022006799A (es) 2022-09-07
AU2020396214B2 (en) 2025-10-30
EP4069834A1 (en) 2022-10-12
JP7702149B2 (ja) 2025-07-03
JP2023504826A (ja) 2023-02-07
EP3831939A1 (en) 2021-06-09

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