AU2020396214B2 - Fusion of site-specific recombinases for efficient and specific genome editing - Google Patents

Fusion of site-specific recombinases for efficient and specific genome editing

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Publication number
AU2020396214B2
AU2020396214B2 AU2020396214A AU2020396214A AU2020396214B2 AU 2020396214 B2 AU2020396214 B2 AU 2020396214B2 AU 2020396214 A AU2020396214 A AU 2020396214A AU 2020396214 A AU2020396214 A AU 2020396214A AU 2020396214 B2 AU2020396214 B2 AU 2020396214B2
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AU
Australia
Prior art keywords
recombinase
seq
sequence
site
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2020396214A
Other languages
English (en)
Other versions
AU2020396214A1 (en
Inventor
Frank Buchholz
Janet Karpinski
Felix LANSING
Liliya MUKHAMETZYANOVA
Teresa ROJO ROMANOS
Jan Sonntag
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technische Universitaet Dresden
Original Assignee
Technische Universitaet Dresden
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Technische Universitaet Dresden filed Critical Technische Universitaet Dresden
Publication of AU2020396214A1 publication Critical patent/AU2020396214A1/en
Application granted granted Critical
Publication of AU2020396214B2 publication Critical patent/AU2020396214B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2020396214A 2019-12-06 2020-12-03 Fusion of site-specific recombinases for efficient and specific genome editing Active AU2020396214B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19214152.1A EP3831939A1 (en) 2019-12-06 2019-12-06 Fusion of site-specific recombinases for efficient and specific genome editing
EP19214152.1 2019-12-06
PCT/EP2020/084489 WO2021110846A1 (en) 2019-12-06 2020-12-03 Fusion of site-specific recombinases for efficient and specific genome editing

Publications (2)

Publication Number Publication Date
AU2020396214A1 AU2020396214A1 (en) 2022-06-16
AU2020396214B2 true AU2020396214B2 (en) 2025-10-30

Family

ID=68808195

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020396214A Active AU2020396214B2 (en) 2019-12-06 2020-12-03 Fusion of site-specific recombinases for efficient and specific genome editing

Country Status (8)

Country Link
US (1) US20250243473A1 (https=)
EP (2) EP3831939A1 (https=)
JP (1) JP7702149B2 (https=)
CN (1) CN115427558A (https=)
AU (1) AU2020396214B2 (https=)
CA (1) CA3163359A1 (https=)
MX (1) MX2022006799A (https=)
WO (1) WO2021110846A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
EP4433584A1 (en) * 2021-11-15 2024-09-25 Technische Universität Dresden Site-specific recombinases for efficient and specific genome editing
US20250145973A1 (en) * 2023-10-16 2025-05-08 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Conditioned dna modifying enzyme comprising heterologous dna binding domain
WO2026022363A1 (en) 2024-07-25 2026-01-29 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Modifying nucleic acid sequences having asymmetric target sites

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999025840A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign dna into eukaryoticgenomes

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981830A (en) 1997-05-30 1999-11-09 Schering Aktiengesellschaft Knockout mice and their progeny with a disrupted hepsin gene
GB0029375D0 (en) * 2000-12-01 2001-01-17 Total Fina Elf Substrate linked directed evolution (slide)
US20060014264A1 (en) * 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
EP1942192A1 (en) 2007-01-08 2008-07-09 Heinrich-Pette-Institut für experimentelle Virologie und Immunologie Use of a tailored recombinase for the treatment of retroviral infections
EP2576798B1 (en) 2010-05-27 2016-04-27 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
CN111254500B (zh) * 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
EP2993229A1 (en) * 2014-09-02 2016-03-09 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
US10017832B2 (en) * 2015-08-25 2018-07-10 Washington University Compositions and methods for site specific recombination at asymmetric sites
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
KR20250133994A (ko) * 2018-05-09 2025-09-09 케로스 테라퓨틱스, 인크. 액티빈 수용체 유형 iia 변이체 및 그의 사용 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999025840A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign dna into eukaryoticgenomes

Also Published As

Publication number Publication date
US20250243473A1 (en) 2025-07-31
WO2021110846A1 (en) 2021-06-10
CA3163359A1 (en) 2021-06-10
AU2020396214A1 (en) 2022-06-16
MX2022006799A (es) 2022-09-07
EP4069834A1 (en) 2022-10-12
CN115427558A (zh) 2022-12-02
JP7702149B2 (ja) 2025-07-03
JP2023504826A (ja) 2023-02-07
EP3831939A1 (en) 2021-06-09

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