MX2022006799A - Fusion de recombinasas especificas del sitio para una edicion genomica eficiente y especifica. - Google Patents
Fusion de recombinasas especificas del sitio para una edicion genomica eficiente y especifica.Info
- Publication number
- MX2022006799A MX2022006799A MX2022006799A MX2022006799A MX2022006799A MX 2022006799 A MX2022006799 A MX 2022006799A MX 2022006799 A MX2022006799 A MX 2022006799A MX 2022006799 A MX2022006799 A MX 2022006799A MX 2022006799 A MX2022006799 A MX 2022006799A
- Authority
- MX
- Mexico
- Prior art keywords
- recombinases
- recombinase
- site
- recombinase enzyme
- fusion
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La invención se refiere, en términos generales, al campo de la edición genómica y proporciona ADN recombinasas, que recombinan de manera eficiente y específica secuencias genómicas con especificidad de objetivo mediante la fusión de monómeros de recombinasa. Más específicamente, la invención proporciona un método para generar una proteína de fusión para la edición eficiente y específica del genoma, que comprende un complejo de recombinasas que comprende al menos una primera enzima recombinasa, una segunda enzima recombinasa y al menos un enlazador, en donde dicha primera enzima recombinasa y dicha segunda enzima recombinasa reconocen específicamente un primer medio sitio y un segundo medio sitio de un sitio objetivo aguas arriba y/o un sitio objetivo aguas abajo de una recombinasa; en donde dicha primera enzima recombinasa y dicha segunda enzima recombinasa están interconectadas a través de un enlazador; y en donde dicho enlazador comprende o consiste en un oligopéptido. La invención se refiere adicionalmente a proteínas de fusión generadas con este método. La invención también describe recombinasas por diseño, que catalizan la inversión de una secuencia de ADN presente en las regiones int1h en el cromosoma X humano. La invención se refiere adicionalmente a moléculas de ácido nucleico que codifican para dichos ADN recombinasas y proteínas de fusión, así como al uso de dichas proteínas de fusión, ADN recombinasas y moléculas de ácido nucleico en una composición farmacéutica.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19214152.1A EP3831939A1 (en) | 2019-12-06 | 2019-12-06 | Fusion of site-specific recombinases for efficient and specific genome editing |
| PCT/EP2020/084489 WO2021110846A1 (en) | 2019-12-06 | 2020-12-03 | Fusion of site-specific recombinases for efficient and specific genome editing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2022006799A true MX2022006799A (es) | 2022-09-07 |
Family
ID=68808195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2022006799A MX2022006799A (es) | 2019-12-06 | 2020-12-03 | Fusion de recombinasas especificas del sitio para una edicion genomica eficiente y especifica. |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP3831939A1 (es) |
| JP (1) | JP2023504826A (es) |
| CN (1) | CN115427558A (es) |
| AU (1) | AU2020396214A1 (es) |
| CA (1) | CA3163359A1 (es) |
| MX (1) | MX2022006799A (es) |
| WO (1) | WO2021110846A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110914414A (zh) | 2017-06-14 | 2020-03-24 | 德累斯顿工业大学 | 利用设计dna重组酶遗传改变基因组的方法与手段 |
| US20230183713A1 (en) * | 2021-11-15 | 2023-06-15 | Technische Universität Dresden | Site-specific recombinases for efficient and specific genome editing |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5981830A (en) | 1997-05-30 | 1999-11-09 | Schering Aktiengesellschaft | Knockout mice and their progeny with a disrupted hepsin gene |
| AU745960C (en) * | 1997-11-18 | 2003-09-18 | Pioneer Hi-Bred International, Inc. | A novel method for the integration of foreign DNA into eukaryoticgenomes |
| EP1942192A1 (en) | 2007-01-08 | 2008-07-09 | Heinrich-Pette-Institut für experimentelle Virologie und Immunologie | Use of a tailored recombinase for the treatment of retroviral infections |
| US20130164271A1 (en) | 2010-05-27 | 2013-06-27 | Max-Planck-Gesellschaft Zur Foederug Der Wissensch E.V. | Tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains |
| SG11201504334WA (en) * | 2012-12-10 | 2015-07-30 | Resolution Bioscience Inc | Methods for targeted genomic analysis |
| EP2993229A1 (en) | 2014-09-02 | 2016-03-09 | Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - | Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains |
| CN110914414A (zh) | 2017-06-14 | 2020-03-24 | 德累斯顿工业大学 | 利用设计dna重组酶遗传改变基因组的方法与手段 |
-
2019
- 2019-12-06 EP EP19214152.1A patent/EP3831939A1/en not_active Withdrawn
-
2020
- 2020-12-03 CA CA3163359A patent/CA3163359A1/en active Pending
- 2020-12-03 MX MX2022006799A patent/MX2022006799A/es unknown
- 2020-12-03 WO PCT/EP2020/084489 patent/WO2021110846A1/en unknown
- 2020-12-03 CN CN202080095838.7A patent/CN115427558A/zh active Pending
- 2020-12-03 EP EP20816214.9A patent/EP4069834A1/en active Pending
- 2020-12-03 AU AU2020396214A patent/AU2020396214A1/en active Pending
- 2020-12-03 JP JP2022533406A patent/JP2023504826A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN115427558A (zh) | 2022-12-02 |
| CA3163359A1 (en) | 2021-06-10 |
| EP3831939A1 (en) | 2021-06-09 |
| JP2023504826A (ja) | 2023-02-07 |
| WO2021110846A1 (en) | 2021-06-10 |
| EP4069834A1 (en) | 2022-10-12 |
| AU2020396214A1 (en) | 2022-06-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cho et al. | Targeted A-to-G base editing in human mitochondrial DNA with programmable deaminases | |
| ES2784754T3 (es) | Métodos y composiciones para modificar un locus objetivo | |
| CN108136047B (zh) | 提高原代细胞中基于核酸内切酶的基因编辑 | |
| ES2666179T3 (es) | Montaje de ADN mediado por nucleasas | |
| van Erp et al. | The history and market impact of CRISPR RNA-guided nucleases | |
| WO2017070632A3 (en) | Nucleobase editors and uses thereof | |
| US20230094769A1 (en) | Inhibition of unintended mutations in gene editing | |
| WO2019139645A3 (en) | High efficiency base editors comprising gam | |
| PH12021550904A1 (en) | Novel crispr/cas12f enzyme and system | |
| PH12021551114A1 (en) | Crispr-cas12j enzyme and system | |
| MX2022006799A (es) | Fusion de recombinasas especificas del sitio para una edicion genomica eficiente y especifica. | |
| GB2556276A (en) | Engineered crispr-CAS9 compositions and methods of use | |
| Peng et al. | Generation of long insert pairs using a Cre-LoxP Inverse PCR approach | |
| Raabe et al. | The rocks and shallows of deep RNA sequencing: Examples in the Vibrio cholerae RNome | |
| CN117321197A (zh) | 背景依赖性、双链dna特异性脱氨酶及其用途 | |
| US20210363206A1 (en) | Proteins that inhibit cas12a (cpf1), a cripr-cas nuclease | |
| CN105154436A (zh) | 包含突变的核酸内切酶识别区dna及其基因组编辑应用 | |
| BR112021007503A2 (pt) | Proteína de fusão, molécula de ácido nucleico, método para edição de material genético, e, sistemas para edição de material genético e para distribuição de componentes de edição de genoma | |
| WO2017058810A3 (en) | Polymerase compositions and kits, and methods of using and making the same | |
| CN105462942B (zh) | Dna聚合酶及其编码基因与应用 | |
| US20230083751A1 (en) | Method For Constructing Gene Mutation Library | |
| RU2771374C1 (ru) | Способы для бесшовного внесения целевых модификаций в направленные векторы | |
| Sridhara et al. | RNA-RNA ligation: Methods, Prospects and Applications | |
| CN107312788A (zh) | 一种tale重复序列载体的构建方法 | |
| RU2008141401A (ru) | Способ повышения эффективности вирусных систем продукции рекомбинантных белков в растениях |