JP7629847B2 - 核酸検出方法 - Google Patents

核酸検出方法 Download PDF

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JP7629847B2
JP7629847B2 JP2021503732A JP2021503732A JP7629847B2 JP 7629847 B2 JP7629847 B2 JP 7629847B2 JP 2021503732 A JP2021503732 A JP 2021503732A JP 2021503732 A JP2021503732 A JP 2021503732A JP 7629847 B2 JP7629847 B2 JP 7629847B2
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stranded
sequence
nucleic acid
oligonucleotide
strand
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JP2021531778A5 (https=
JPWO2020021272A5 (https=
JP2021531778A (ja
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ジョン ランブレ ヘンリー
ロイド デイビッド
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Sense Biodetection Ltd
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JP2021503732A 2018-07-25 2019-07-25 核酸検出方法 Active JP7629847B2 (ja)

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GBGB1812149.1A GB201812149D0 (en) 2018-07-25 2018-07-25 Nucleic acid detection method
GB1812149.1 2018-07-25
PCT/GB2019/052089 WO2020021272A1 (en) 2018-07-25 2019-07-25 Nucleic acid detection method

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JP2021531778A JP2021531778A (ja) 2021-11-25
JP2021531778A5 JP2021531778A5 (https=) 2022-04-07
JPWO2020021272A5 JPWO2020021272A5 (https=) 2022-04-07
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US (4) US20210246487A1 (https=)
EP (2) EP4647507A3 (https=)
JP (2) JP7629847B2 (https=)
KR (1) KR20210038541A (https=)
CN (1) CN112469833A (https=)
AU (2) AU2019311340B2 (https=)
BR (1) BR112021000574A2 (https=)
CA (1) CA3107388A1 (https=)
ES (1) ES3040542T3 (https=)
GB (2) GB201812149D0 (https=)
MX (1) MX2021000946A (https=)
WO (1) WO2020021272A1 (https=)
ZA (1) ZA202007778B (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
US20210040571A1 (en) * 2018-07-25 2021-02-11 Sense Biodetection Limited Nucleic acid detection method
GB201812149D0 (en) * 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method
EP4196787A1 (en) 2020-08-11 2023-06-21 Zoetis Services LLC Lateral flow device for detecting sars-cov-2 antibodies in human and animal samples
JP2023539299A (ja) 2020-08-26 2023-09-13 センス バイオディテクション リミテッド デバイス
WO2022043697A1 (en) 2020-08-26 2022-03-03 Sense Biodetection Limited Devices
CN113624980B (zh) * 2021-08-09 2023-06-13 四川大学华西医院 基于识别诱导的恒温扩增技术对蛋白质进行检测的方法及试剂盒
CN114107510B (zh) * 2021-12-10 2023-10-03 湖南工程学院 基于dna三链介导构建多维dna酶矩阵的超灵敏循环核酸检测体系、试剂盒和方法
CN117568446A (zh) * 2022-08-08 2024-02-20 台达电子国际（新加坡）私人有限公司 检测病毒感染性的样本前处理试剂盒及方法
CN116162693B (zh) * 2022-08-12 2025-08-01 浙江大学 一种检测阿尔茨海默病致病基因突变和apoe基因型的引物组合及试剂盒
CN115747322B (zh) * 2022-08-12 2025-08-01 浙江大学 一种恒温多重扩增检测肝豆状核变性atp7b基因高频突变位点基因分型的检测方法
WO2024072805A1 (en) * 2022-09-26 2024-04-04 Lau, Johnson Yiu-Nam Compositions, systems, and methods for detection of ovarian cancer
WO2024080776A1 (ko) * 2022-10-14 2024-04-18 고려대학교 산학협력단 비색 검출을 위한 황 함유 프로브 및 핵산 내 황 도입을 통한 효소 활성 가속화 방법
CN116536400A (zh) * 2023-04-06 2023-08-04 广州迪澳基因科技有限公司 一种高特异性等温核酸扩增方法
CN117046441B (zh) * 2023-08-14 2024-04-02 中国科学院生态环境研究中心 磁性氧化石墨烯粒子、制备方法及其应用
WO2025255157A1 (en) * 2024-06-04 2025-12-11 President And Fellows Of Harvard College Systems, methods, and compositions for protein screening
WO2026015653A2 (en) 2024-07-09 2026-01-15 Sherlock Biosciences, Inc. Power-free fluidic device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005537002A (ja) 2002-09-02 2005-12-08 パムジーン ベー．ベー． 新規な一体化したマイクロアレイ分析

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US6136533A (en) 1997-07-03 2000-10-24 Id Biomedical Additives for use in cycling probe reactions
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US20100279295A1 (en) 2009-03-18 2010-11-04 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
UA116205C2 (uk) 2012-04-09 2018-02-26 Енвіролоджикс Інк. Спосіб кількісного визначення послідовності нуклеїнової кислоти в зразку
US10590474B2 (en) * 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
EP3825411A1 (en) * 2014-06-18 2021-05-26 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
US10450562B2 (en) 2014-09-09 2019-10-22 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
US10337072B2 (en) * 2015-01-08 2019-07-02 The Board Of Trustees Of The University Of Illinois Copy number detection and methods
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
WO2018114674A1 (de) * 2016-12-23 2018-06-28 Albert-Ludwigs-Universität Freiburg Zweiteilige mediatorsonde
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201812149D0 (en) * 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005537002A (ja) 2002-09-02 2005-12-08 パムジーン ベー．ベー． 新規な一体化したマイクロアレイ分析

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Analyst, 2015, Vol.140, pp.7540-7549，https://doi.org/10.1039/C5AN01632K
Christoph Kessler (ed.)，Nonradioactive analysis ofbiomolecules- 2nd ed.，2000, Springer-Verlag Berlin Heidelberg, Chapter 29,pp.356-366，ISBN 978-3-540-64601-3, ISBN 978-3-642-57206-7 (eBook), DOI10.1007/978-3-642-57206-7
Molecular BioSystems, 2014, Vol.10, pp.970-1003，DOI: 10.1039/c3mb70304e

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