JP7625519B2 - ウイルスの製造 - Google Patents

ウイルスの製造 Download PDF

Info

Publication number
JP7625519B2
JP7625519B2 JP2021528362A JP2021528362A JP7625519B2 JP 7625519 B2 JP7625519 B2 JP 7625519B2 JP 2021528362 A JP2021528362 A JP 2021528362A JP 2021528362 A JP2021528362 A JP 2021528362A JP 7625519 B2 JP7625519 B2 JP 7625519B2
Authority
JP
Japan
Prior art keywords
virus
hours
pfu
cell
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021528362A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022508156A5 (https=
JP2022508156A (ja
Inventor
ポール アンドリュー ライト，
ケイティ ワック，
ミンルイ ジャン，
ジャッキー ヴァスール，
スーザン ニコル ホリー，
オーガスト アレン レンショー，
グワンユー ジュー，
ミシェル キャサリン ヴェンデル，
Original Assignee
カリヴィル イムノセラピューティクス， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by カリヴィル イムノセラピューティクス， インコーポレイテッド filed Critical カリヴィル イムノセラピューティクス， インコーポレイテッド
Publication of JP2022508156A publication Critical patent/JP2022508156A/ja
Publication of JP2022508156A5 publication Critical patent/JP2022508156A5/ja
Application granted granted Critical
Publication of JP7625519B2 publication Critical patent/JP7625519B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021528362A 2018-11-21 2019-11-21 ウイルスの製造 Active JP7625519B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862770577P 2018-11-21 2018-11-21
US62/770,577 2018-11-21
PCT/US2019/062643 WO2020106975A1 (en) 2018-11-21 2019-11-21 Manufacture of virus

Publications (3)

Publication Number Publication Date
JP2022508156A JP2022508156A (ja) 2022-01-19
JP2022508156A5 JP2022508156A5 (https=) 2022-11-22
JP7625519B2 true JP7625519B2 (ja) 2025-02-03

Family

ID=70774620

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021528362A Active JP7625519B2 (ja) 2018-11-21 2019-11-21 ウイルスの製造

Country Status (8)

Country Link
US (1) US20210277363A1 (https=)
EP (1) EP3884044A4 (https=)
JP (1) JP7625519B2 (https=)
KR (1) KR20210093965A (https=)
CN (1) CN113330111A (https=)
AU (1) AU2019384164A1 (https=)
CA (1) CA3120129A1 (https=)
WO (1) WO2020106975A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4222250A1 (en) * 2020-10-02 2023-08-09 Genelux Corporation Bioreactor production of virus from adherent cells
CN121219392A (zh) * 2023-05-31 2025-12-26 康宁股份有限公司 用于提高固定床生物反应器的细胞收集效率的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008067720A (ja) 1997-01-31 2008-03-27 Schering Corp 細胞培養およびウイルス増殖のための方法
JP2010526546A (ja) 2007-05-14 2010-08-05 バヴァリアン・ノルディック・アクティーゼルスカブ ワクシニアウイルス系及び組換えワクシニアウイルス系ワクチンの精製
JP2014521353A (ja) 2011-08-05 2014-08-28 ジェンネレックス インコーポレイティッド ワクシニアウィルスの生成のための方法および組成物

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2287288B1 (en) * 2002-07-09 2012-11-07 Baxter International Inc. Animal protein free media for cultivation of cells
US20070207461A1 (en) * 2004-02-23 2007-09-06 Crucell Holland B.V. Virus Purification Methods
US8541559B2 (en) * 2006-06-12 2013-09-24 Cytos Biotechnology Ag Process for producing aggregated oligonucleotides
EP2615167A1 (en) * 2006-09-15 2013-07-17 MedImmune, LLC Method for eliminating DNA contaminants from viral preparations
US8003363B2 (en) * 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
US8785173B2 (en) * 2008-09-24 2014-07-22 Medimmune, Llc Methods for purification of viruses
US10851350B1 (en) * 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008067720A (ja) 1997-01-31 2008-03-27 Schering Corp 細胞培養およびウイルス増殖のための方法
JP2010526546A (ja) 2007-05-14 2010-08-05 バヴァリアン・ノルディック・アクティーゼルスカブ ワクシニアウイルス系及び組換えワクシニアウイルス系ワクチンの精製
JP2014521353A (ja) 2011-08-05 2014-08-28 ジェンネレックス インコーポレイティッド ワクシニアウィルスの生成のための方法および組成物

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BMC Proceedings，2013, Vol.7, Suppl.6, #P60, pp.1-3
Journal of Virological Methods，2017, Vol.243, pp.68-73

Also Published As

Publication number Publication date
CA3120129A1 (en) 2020-05-28
EP3884044A1 (en) 2021-09-29
CN113330111A (zh) 2021-08-31
US20210277363A1 (en) 2021-09-09
KR20210093965A (ko) 2021-07-28
JP2022508156A (ja) 2022-01-19
WO2020106975A1 (en) 2020-05-28
AU2019384164A1 (en) 2021-06-03
EP3884044A4 (en) 2022-08-31

Similar Documents

Publication Publication Date Title
JP7685543B2 (ja) 細胞培養物を処理する方法およびシステム
JP6385559B2 (ja) 細胞培養体からのポリオウイルスの精製プロセス
JP7625519B2 (ja) ウイルスの製造
US20250230419A1 (en) Methods of purifying an enveloped virus
CN104027800A (zh) 一种人用狂犬病疫苗的制备方法
KR102245547B1 (ko) 고밀도의 미정제 세포 배양 수거물의 정화를 위한 방법
JP2025118611A (ja) 接着細胞からのウイルスのバイオリアクター生成
US20140309408A1 (en) Method for purifying virus-like particles (vlp)
CN113151197A (zh) 一种Vero细胞培养流感病毒的纯化方法
HK40058237A (en) Manufacture of virus
CN113825835B (zh) 使用渗滤方法有效去除杂质
CN112469821B (zh) 一种制备重组人凝血因子ⅷ的方法
Vergauwen et al. Filtration Principles and Techniques for Bioprocessing of Viral Vector-Based Therapeutics
CN105316296A (zh) 一种纯化腺病毒颗粒的方法
CN117660373A (zh) 一种慢病毒大规模纯化方法及其应用

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221114

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20221114

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20231019

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20231025

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20240115

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240227

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240528

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20240821

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20240905

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20240905

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241028

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20241225

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250122

R150 Certificate of patent or registration of utility model

Ref document number: 7625519

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150