CN113330111A - 病毒的制造 - Google Patents

病毒的制造 Download PDF

Info

Publication number
CN113330111A
CN113330111A CN201980089784.0A CN201980089784A CN113330111A CN 113330111 A CN113330111 A CN 113330111A CN 201980089784 A CN201980089784 A CN 201980089784A CN 113330111 A CN113330111 A CN 113330111A
Authority
CN
China
Prior art keywords
virus
culture
pfu
recombinant oncolytic
bioreactor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980089784.0A
Other languages
English (en)
Chinese (zh)
Inventor
保罗·安德鲁·赖特
凯蒂·瓦克
张明瑞
杰基·瓦瑟尔
苏珊·妮可·霍利
奥古斯特·艾伦·伦肖
朱广宇
米歇尔·凯瑟琳·文德尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliwell Immunotherapy
Original Assignee
Western Oncolytics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Western Oncolytics Ltd filed Critical Western Oncolytics Ltd
Publication of CN113330111A publication Critical patent/CN113330111A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980089784.0A 2018-11-21 2019-11-21 病毒的制造 Pending CN113330111A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862770577P 2018-11-21 2018-11-21
US62/770,577 2018-11-21
PCT/US2019/062643 WO2020106975A1 (en) 2018-11-21 2019-11-21 Manufacture of virus

Publications (1)

Publication Number Publication Date
CN113330111A true CN113330111A (zh) 2021-08-31

Family

ID=70774620

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980089784.0A Pending CN113330111A (zh) 2018-11-21 2019-11-21 病毒的制造

Country Status (8)

Country Link
US (1) US20210277363A1 (https=)
EP (1) EP3884044A4 (https=)
JP (1) JP7625519B2 (https=)
KR (1) KR20210093965A (https=)
CN (1) CN113330111A (https=)
AU (1) AU2019384164A1 (https=)
CA (1) CA3120129A1 (https=)
WO (1) WO2020106975A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4222250A1 (en) * 2020-10-02 2023-08-09 Genelux Corporation Bioreactor production of virus from adherent cells
CN121219392A (zh) * 2023-05-31 2025-12-26 康宁股份有限公司 用于提高固定床生物反应器的细胞收集效率的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1922308A (zh) * 2004-02-23 2007-02-28 克鲁塞尔荷兰公司 病毒纯化方法
CN101058800A (zh) * 2002-07-09 2007-10-24 巴克斯特国际有限公司 用于细胞培养的无动物蛋白质培养基
US20080286850A1 (en) * 2006-09-15 2008-11-20 Medimmune Vaccines, Inc. Mdck cell lines supporting viral growth to high titers and bioreactor process using the same
CN101466720A (zh) * 2006-06-12 2009-06-24 赛托斯生物技术公司 向rna噬菌体的病毒样颗粒内包装寡核苷酸的方法
US20140162342A1 (en) * 2011-08-05 2014-06-12 Jennerex, Inc. Methods and compositions for production of vaccina virus
US10851350B1 (en) * 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0981601B1 (en) 1997-01-31 2006-09-06 Schering Corporation Methods for cultivating cells and propagating viruses
JP2010526546A (ja) 2007-05-14 2010-08-05 バヴァリアン・ノルディック・アクティーゼルスカブ ワクシニアウイルス系及び組換えワクシニアウイルス系ワクチンの精製
US8003363B2 (en) * 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
US8785173B2 (en) * 2008-09-24 2014-07-22 Medimmune, Llc Methods for purification of viruses

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101058800A (zh) * 2002-07-09 2007-10-24 巴克斯特国际有限公司 用于细胞培养的无动物蛋白质培养基
CN1922308A (zh) * 2004-02-23 2007-02-28 克鲁塞尔荷兰公司 病毒纯化方法
CN101466720A (zh) * 2006-06-12 2009-06-24 赛托斯生物技术公司 向rna噬菌体的病毒样颗粒内包装寡核苷酸的方法
US20080286850A1 (en) * 2006-09-15 2008-11-20 Medimmune Vaccines, Inc. Mdck cell lines supporting viral growth to high titers and bioreactor process using the same
US20140162342A1 (en) * 2011-08-05 2014-06-12 Jennerex, Inc. Methods and compositions for production of vaccina virus
US10851350B1 (en) * 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Benzonase Nuclease高活力全能核酸酶", pages 2, Retrieved from the Internet <URL:https://www.biomart.cn/news/78/79/2834461.htm> *
MAFALDA G. MOLEIRINHO等: "Clinical-grade Oncolytic Adenovirus Purification Using Polysorbate 20 as an Alternative for Cell Lysis", CURRENT GENE THERAPY, 31 December 2018 (2018-12-31), pages 366 - 374 *
ZONG SHENG GUO等: "Rapid Generation of Multiple Loci-Engineered Marker-free Poxvirus and Characterization of a Clinical-Grade Oncolytic Vaccinia Virus", MOLECULAR THERAPY: METHODS & CLINICAL DEVELOPMENT, 31 December 2017 (2017-12-31), pages 120 *

Also Published As

Publication number Publication date
JP7625519B2 (ja) 2025-02-03
CA3120129A1 (en) 2020-05-28
EP3884044A1 (en) 2021-09-29
US20210277363A1 (en) 2021-09-09
KR20210093965A (ko) 2021-07-28
JP2022508156A (ja) 2022-01-19
WO2020106975A1 (en) 2020-05-28
AU2019384164A1 (en) 2021-06-03
EP3884044A4 (en) 2022-08-31

Similar Documents

Publication Publication Date Title
JP7008746B2 (ja) 細胞培養物を処理する方法およびシステム
US11629339B2 (en) Aseptic purification process for viruses
JP6385559B2 (ja) 細胞培養体からのポリオウイルスの精製プロセス
HK1248763A1 (en) Aseptic purification process for viruses
CN113330111A (zh) 病毒的制造
CN104027797B (zh) 一种白喉疫苗的制备方法
CN118974245A (zh) 纯化包膜病毒的方法
CN104027800A (zh) 一种人用狂犬病疫苗的制备方法
JP2025118611A (ja) 接着細胞からのウイルスのバイオリアクター生成
US20210268405A1 (en) Method for producing recombinant adenovirus
US20260085294A1 (en) Process for producing a purified rhabdovirus from cell culture
HK40058237A (en) Manufacture of virus
JP2022508156A5 (https=)
CN113825835B (zh) 使用渗滤方法有效去除杂质
CN112469821B (zh) 一种制备重组人凝血因子ⅷ的方法
EP2603234B1 (en) Method for reducing dna impurities in viral compositions
CN121699879A (zh) 重组脊髓灰质炎病毒制备方法
CN105316296A (zh) 一种纯化腺病毒颗粒的方法
CN114272366A (zh) 一种制备人用乙型脑炎灭活疫苗的方法及疫苗
EA049917B1 (ru) Способ получения очищенного рабдовируса из клеточной культуры
HK1237812A1 (en) Process for the purification of poliovirus from cell cultures

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40058237

Country of ref document: HK

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20220601

Address after: Pennsylvania, America

Applicant after: Caliwell immunotherapy

Address before: Pennsylvania, America

Applicant before: Western oncolytic cell Co.,Ltd.

CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Paul Andrew Wright

Inventor after: Katie Walker

Inventor after: Zhang Mingrui

Inventor after: Jackie Wassel

Inventor after: Susan Nicole Holly

Inventor after: August Allen Renshaw

Inventor after: Zhu Guangyu

Inventor after: Michelle Catherine Wendell

Inventor before: Paul Andrew Wright

Inventor before: Katie Walker

Inventor before: Zhang Mingrui

Inventor before: Jackie Wassel

Inventor before: Susan Nicole Holly

Inventor before: August Allen Renshaw

Inventor before: Zhu Guangyu

Inventor before: Michelle Catherine Wendell

WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20210831