JP7530554B2 - 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法 - Google Patents

3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法 Download PDF

Info

Publication number
JP7530554B2
JP7530554B2 JP2020546523A JP2020546523A JP7530554B2 JP 7530554 B2 JP7530554 B2 JP 7530554B2 JP 2020546523 A JP2020546523 A JP 2020546523A JP 2020546523 A JP2020546523 A JP 2020546523A JP 7530554 B2 JP7530554 B2 JP 7530554B2
Authority
JP
Japan
Prior art keywords
coa
acid
seq
genetically modified
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020546523A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2020230718A1 (https=
JPWO2020230718A5 (https=
Inventor
匡平 磯部
健司 河村
勝成 山田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Publication of JPWO2020230718A1 publication Critical patent/JPWO2020230718A1/ja
Publication of JPWO2020230718A5 publication Critical patent/JPWO2020230718A5/ja
Application granted granted Critical
Publication of JP7530554B2 publication Critical patent/JP7530554B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42Hydroxy-carboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/32Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44Polycarboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/011573-Hydroxybutyryl-CoA dehydrogenase (1.1.1.157)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185Escherichia
    • C12R2001/19Escherichia coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/425Serratia

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020546523A 2019-05-10 2020-05-08 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法 Active JP7530554B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019089770 2019-05-10
JP2019089770 2019-05-10
PCT/JP2020/018663 WO2020230718A1 (ja) 2019-05-10 2020-05-08 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Publications (3)

Publication Number Publication Date
JPWO2020230718A1 JPWO2020230718A1 (https=) 2020-11-19
JPWO2020230718A5 JPWO2020230718A5 (https=) 2023-05-08
JP7530554B2 true JP7530554B2 (ja) 2024-08-08

Family

ID=73290002

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020546523A Active JP7530554B2 (ja) 2019-05-10 2020-05-08 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Country Status (5)

Country Link
US (1) US20220213514A1 (https=)
EP (1) EP3967762A4 (https=)
JP (1) JP7530554B2 (https=)
CN (1) CN113785069A (https=)
WO (1) WO2020230718A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12275979B2 (en) * 2017-11-30 2025-04-15 Toray Industries, Inc. Gene-modified microorganism for producing 3-hydroxyadipic acid, alpha-hydromuconic acid, and/or adipic acid, and production method for said chemical products
US20230392112A1 (en) * 2020-11-11 2023-12-07 Toray Industries, Inc. Genetically modified microorganism for producing 3-hydroxyadipic acid and/or alpha-hydromuconic acid, and method for producing chemical product
US20250382643A1 (en) 2022-02-15 2025-12-18 Toray Industries, Inc. GENETICALLY MODIFIED MICROORGANISM FOR PRODUCING 3-HYDROXYADIPIC ACID AND/OR Alpha-HYDROXYADIPIC ACID, AND METHOD FOR PRODUCING CHEMICAL PRODUCT
CN115287238B (zh) * 2022-09-09 2023-04-14 哈尔滨工业大学 一种粘质沙雷氏菌株及其应用
CN121057821A (zh) * 2023-05-23 2025-12-02 东丽株式会社 用于生产3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的基因修饰微生物以及该化学品的制造方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011515111A (ja) 2008-03-27 2011-05-19 ジェノマティカ, インコーポレイテッド アジピン酸および他の化合物を生成するための微生物
JP2013535203A (ja) 2010-07-26 2013-09-12 ジェノマティカ・インコーポレイテッド 芳香族、2,4−ペンタジエノエートおよび1,3−ブタジエンを生合成するための微生物および方法
WO2017033965A1 (ja) 2015-08-24 2017-03-02 国立研究開発法人理化学研究所 芳香族化合物及びその誘導体の製造方法
JP2017184746A (ja) 2012-01-30 2017-10-12 ミリアント・コーポレイションMyriant Corporation 遺伝子操作された微生物からのムコン酸の生成
WO2017209103A1 (ja) 2016-05-31 2017-12-07 東レ株式会社 α-ヒドロムコン酸の製造方法
WO2017209102A1 (ja) 2016-05-31 2017-12-07 東レ株式会社 3-ヒドロキシアジピン酸の製造方法
JP2018500911A (ja) 2014-12-23 2018-01-18 ジェノマティカ, インコーポレイテッド ジアミンの製造および処理方法
WO2019022083A1 (ja) 2017-07-24 2019-01-31 国立研究開発法人理化学研究所 デカルボキシラーゼ、及びそれを用いた不飽和炭化水素化合物の製造方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100630836B1 (ko) 2005-04-08 2006-10-02 한국과학기술원 인-실리코 분석을 통한 균주 개량방법
DK2427544T3 (da) 2009-05-07 2019-10-07 Genomatica Inc Mikroorganismer og fremgangsmåder til biosyntese af adipat, hexamethylendiamin og 6-aminokapronsyre
WO2011017560A1 (en) 2009-08-05 2011-02-10 Genomatica, Inc. Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
US9187772B2 (en) * 2010-09-01 2015-11-17 University Of Florida Research Foundation, Inc. L-malate production by metabolically engineered escherichia coli
TWI716752B (zh) * 2012-12-17 2021-01-21 美商奇諾麥提卡公司 用於增加甲醇存在下之還原當量可利用性及用於製造與其相關己二酸、6-胺基己酸、己二胺或己內醯胺之微生物及方法
CN107614692B (zh) 2015-06-10 2022-04-19 东丽株式会社 α-氢化己二烯二酸的制造方法
EP3309258B1 (en) 2015-06-10 2020-06-03 Toray Industries, Inc. Method for producing 3-hydroxyadipic acid
EP3388525B1 (en) 2015-12-11 2020-09-16 Toray Industries, Inc. Method for producing 3-oxoadipic acid by fermentation
US12275979B2 (en) * 2017-11-30 2025-04-15 Toray Industries, Inc. Gene-modified microorganism for producing 3-hydroxyadipic acid, alpha-hydromuconic acid, and/or adipic acid, and production method for said chemical products

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011515111A (ja) 2008-03-27 2011-05-19 ジェノマティカ, インコーポレイテッド アジピン酸および他の化合物を生成するための微生物
JP2013535203A (ja) 2010-07-26 2013-09-12 ジェノマティカ・インコーポレイテッド 芳香族、2,4−ペンタジエノエートおよび1,3−ブタジエンを生合成するための微生物および方法
JP2017184746A (ja) 2012-01-30 2017-10-12 ミリアント・コーポレイションMyriant Corporation 遺伝子操作された微生物からのムコン酸の生成
JP2018500911A (ja) 2014-12-23 2018-01-18 ジェノマティカ, インコーポレイテッド ジアミンの製造および処理方法
WO2017033965A1 (ja) 2015-08-24 2017-03-02 国立研究開発法人理化学研究所 芳香族化合物及びその誘導体の製造方法
WO2017209103A1 (ja) 2016-05-31 2017-12-07 東レ株式会社 α-ヒドロムコン酸の製造方法
WO2017209102A1 (ja) 2016-05-31 2017-12-07 東レ株式会社 3-ヒドロキシアジピン酸の製造方法
WO2019022083A1 (ja) 2017-07-24 2019-01-31 国立研究開発法人理化学研究所 デカルボキシラーゼ、及びそれを用いた不飽和炭化水素化合物の製造方法

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Database GenBank [online],Accession No. ABV40935.1,3-hydroxybutyryl-CoA dehydrogenase [Serratia proteamaculans 568],2014年01月28日,インターネット <URL: https://www.ncbi.nlm.gov/protein/ABV40935.1>,[検索日 2019.02.06]
Database GenBank [online],Accession No. AGP43956.1,3-hydroxybutyryl-CoA dehydrogenase [Serratia plymuthica S13],2014年01月30日,インターネット <URL: https://www.ncbi.nlm.gov/protein/AGP43956.1>,[検索日 2019.02.06]
Database GenBank [online],Accession No. ALL37524.1,3-hydroxybutyryl-CoA dehydrogenase [Serratia marcescens],2017年02月28日,インターネット <URL: https://www.ncbi.nlm.nih.gov/protein/943365850>,[検索日 2019.02.06]
Database GenBank [online],Accession No. AMG98270.1,3-hydroxybutyryl-CoA dehydrogenase [Serratia liquwfaciens],2016年02月11日,インターネット <URL: https://www.ncbi. nlm.gov/protein/991935067?sat=4&satkey=158110186>,[検索日 2019.02.06]
Database GenBank [online],Accession No. ASM11476.1,3-hydroxybutyryl-CoA dehydrogenase [Serratia marcescens],2017年07月18日,インターネット <URL: https://www.ncbi.nlm.gov/protein/ASM11476.1>,[検索日 2019.02.06]
Database GenBank [online],Accession No. KFF90258.1,3-hydroxybutyryl-CoA dehydrogenase [Serratia nematodiphila DZ0503SBS1],2014年08月07日,インターネット <URL: https://www.ncbi.nlm.gov/protein/KFF90258.1>,[検索日 2019.02.06]
Database GenBank [online],Accession No. SMB31239.1,3-hydroxybutyryl-CoA dehydrogenase [Serratia proteamaculans],2017年04月14日,インターネット <URL: https://www.ncbi.nlm.gov/protein/SMB31239.1>,[検索日 2019.02.06]

Also Published As

Publication number Publication date
EP3967762A4 (en) 2023-07-19
JPWO2020230718A1 (https=) 2020-11-19
US20220213514A1 (en) 2022-07-07
CN113785069A (zh) 2021-12-10
WO2020230718A1 (ja) 2020-11-19
EP3967762A1 (en) 2022-03-16

Similar Documents

Publication Publication Date Title
JP7331691B2 (ja) 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP7530554B2 (ja) 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
US11078503B2 (en) Method for producing 3-hydroxyadipic acid
CN108467848A (zh) 可利用木糖的棒状杆菌微生物和利用其产生l-赖氨酸的方法
JP7646119B2 (ja) 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP7784060B2 (ja) 3-ヒドロキシアジピン酸および/またはα-ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
US11248207B2 (en) Method for producing alpha-hydromuconic acid
US20230392112A1 (en) Genetically modified microorganism for producing 3-hydroxyadipic acid and/or alpha-hydromuconic acid, and method for producing chemical product
US20250215461A1 (en) Genetically modified microorganism for producing 3-hydroxyadipic acid and/or 3-oxoadipic acid, and production method for said chemical products
WO2023157816A1 (ja) 3-ヒドロキシアジピン酸および/またはα-ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP7769868B2 (ja) 遺伝子改変微生物および有機酸の製造方法
KR20250137042A (ko) 증대된 갈산(gallic acid) 생산능을 갖는 재조합 에스케리키아속 미생물 및 이를 이용한 갈산의 생산방법
WO2025063258A1 (ja) α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP2024090463A (ja) 3-ヒドロキシアジピン酸および/またはα-ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
JP2023144366A (ja) 3-ヒドロキシアジピン酸および/またはα-ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法
WO2022181654A1 (ja) 変異型アシル-CoAヒドロラーゼ
WO2024242124A1 (ja) 3-ヒドロキシアジピン酸、α-ヒドロムコン酸および/またはアジピン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230425

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230425

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240322

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240520

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20240627

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20240710

R150 Certificate of patent or registration of utility model

Ref document number: 7530554

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150