JP7453788B2 - 抗体製造の間のピンク色の形成を制御する方法 - Google Patents

抗体製造の間のピンク色の形成を制御する方法 Download PDF

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JP7453788B2
JP7453788B2 JP2019561819A JP2019561819A JP7453788B2 JP 7453788 B2 JP7453788 B2 JP 7453788B2 JP 2019561819 A JP2019561819 A JP 2019561819A JP 2019561819 A JP2019561819 A JP 2019561819A JP 7453788 B2 JP7453788 B2 JP 7453788B2
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antibody
cbl
mab
hydrogen peroxide
collection
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JP2020519265A (ja
JP2020519265A5 (https=
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チェン・ドゥ
アメヤ・ウメシュ・ボルワンカル
ジュン・チョン・イー
タン・ジジュン
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Bristol Myers Squibb Co
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Bristol Myers Squibb Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2019561819A 2017-05-09 2018-05-08 抗体製造の間のピンク色の形成を制御する方法 Active JP7453788B2 (ja)

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JP2023145991A JP2023182593A (ja) 2017-05-09 2023-09-08 抗体製造の間のピンク色の形成を制御する方法

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US201762503615P 2017-05-09 2017-05-09
US62/503,615 2017-05-09
PCT/US2018/031542 WO2018208743A1 (en) 2017-05-09 2018-05-08 Method of controlling the formation of pink color during antibody manufacturing

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JP2020519265A JP2020519265A (ja) 2020-07-02
JP2020519265A5 JP2020519265A5 (https=) 2021-05-13
JP7453788B2 true JP7453788B2 (ja) 2024-03-21

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JP2023145991A Withdrawn JP2023182593A (ja) 2017-05-09 2023-09-08 抗体製造の間のピンク色の形成を制御する方法

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US (1) US12241107B2 (https=)
EP (1) EP3621989B1 (https=)
JP (2) JP7453788B2 (https=)
KR (1) KR102664829B1 (https=)
CN (1) CN110831964A (https=)
ES (1) ES2951287T3 (https=)
WO (1) WO2018208743A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7621284B2 (ja) 2020-01-31 2025-01-24 中外製薬株式会社 着色を抑制したポリペプチドを含む組成物の製造方法
KR20250069604A (ko) * 2022-09-15 2025-05-19 사노피 단백질 환원 민감성의 결정 방법

Citations (1)

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Publication number Priority date Publication date Assignee Title
JP2010533192A (ja) 2007-07-09 2010-10-21 ジェネンテック， インコーポレイテッド ポリペプチドの組換え生産中におけるジスルフィド結合還元の防止

Family Cites Families (7)

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JP2689458B2 (ja) * 1988-02-09 1997-12-10 武田薬品工業株式会社 粒状ないし粉末状のビタミンｂ▲下１▼▲下２▼類含有組成物
JP2734709B2 (ja) * 1990-01-05 1998-04-02 東レ株式会社 インクジェット染色用布帛およびそれを用いてなるインクジェット染色法
SE525083C2 (sv) * 2003-04-23 2004-11-23 Kemira Kemi Ab Sätt att behandla rötslam
AU2005310305A1 (en) * 2004-10-22 2006-06-08 Amgen Inc. Methods for refolding polypeptides
EP1971488A2 (en) * 2005-12-05 2008-09-24 Lifeline Cell Technology Cell culture medium container assembly
CA2765220A1 (en) 2009-07-14 2011-01-20 Biogen Idec Ma Inc. Methods for inhibiting yellow color and peroxide formation in a composition
KR20160088930A (ko) 2013-12-04 2016-07-26 이뮤노젠 아이엔씨 항체 생산용 조성물 및 항체 생성 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010533192A (ja) 2007-07-09 2010-10-21 ジェネンテック， インコーポレイテッド ポリペプチドの組換え生産中におけるジスルフィド結合還元の防止

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Hydroxocobalamin association during cell culture results in pink therapeutic proteins，mAbs，2013年，Volume 5, Issue 6，pp. 974-981
Red colored IgG4 caused by vitamin B12 from cell culture media combined with disulfide reduction at harvest，mAbs，2014年，Volume 6, Issue 3，pp. 679-688

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ES2951287T3 (es) 2023-10-19
WO2018208743A9 (en) 2019-12-05
EP3621989B1 (en) 2023-06-28
US12241107B2 (en) 2025-03-04
WO2018208743A1 (en) 2018-11-15
JP2023182593A (ja) 2023-12-26
JP2020519265A (ja) 2020-07-02
EP3621989A1 (en) 2020-03-18
CN110831964A (zh) 2020-02-21
KR102664829B1 (ko) 2024-05-09
US20230103511A1 (en) 2023-04-06
KR20200004355A (ko) 2020-01-13

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