JP7389020B2 - 幹細胞からインビトロで誘導されたnkx6.1およびc-ペプチド共発現細胞の濃縮 - Google Patents
幹細胞からインビトロで誘導されたnkx6.1およびc-ペプチド共発現細胞の濃縮 Download PDFInfo
- Publication number
- JP7389020B2 JP7389020B2 JP2020512652A JP2020512652A JP7389020B2 JP 7389020 B2 JP7389020 B2 JP 7389020B2 JP 2020512652 A JP2020512652 A JP 2020512652A JP 2020512652 A JP2020512652 A JP 2020512652A JP 7389020 B2 JP7389020 B2 JP 7389020B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- endocrine
- peptide
- cryopreserved
- expressing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000004027 cell Anatomy 0.000 title claims description 398
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 title claims description 189
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 title claims description 189
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 title claims description 189
- VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 title claims description 151
- 108010075254 C-Peptide Proteins 0.000 title claims description 148
- 210000000130 stem cell Anatomy 0.000 title claims description 92
- 238000000338 in vitro Methods 0.000 title description 38
- 238000000034 method Methods 0.000 claims description 183
- 210000003890 endocrine cell Anatomy 0.000 claims description 136
- 101100518002 Danio rerio nkx2.2a gene Proteins 0.000 claims description 66
- 108700014808 Homeobox Protein Nkx-2.2 Proteins 0.000 claims description 66
- 102100027886 Homeobox protein Nkx-2.2 Human genes 0.000 claims description 66
- 101100460496 Homo sapiens NKX2-2 gene Proteins 0.000 claims description 66
- 230000002124 endocrine Effects 0.000 claims description 52
- 239000012595 freezing medium Substances 0.000 claims description 26
- 238000010257 thawing Methods 0.000 claims description 25
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 15
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 8
- 238000005138 cryopreservation Methods 0.000 description 50
- 238000010494 dissociation reaction Methods 0.000 description 40
- 230000005593 dissociations Effects 0.000 description 40
- 238000002054 transplantation Methods 0.000 description 38
- 230000014509 gene expression Effects 0.000 description 30
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 29
- 239000008103 glucose Substances 0.000 description 29
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 28
- 206010012601 diabetes mellitus Diseases 0.000 description 21
- 230000004069 differentiation Effects 0.000 description 19
- 239000000203 mixture Substances 0.000 description 18
- 210000002660 insulin-secreting cell Anatomy 0.000 description 17
- 102000004190 Enzymes Human genes 0.000 description 14
- 108090000790 Enzymes Proteins 0.000 description 14
- 102000004877 Insulin Human genes 0.000 description 14
- 108090001061 Insulin Proteins 0.000 description 14
- 229940088598 enzyme Drugs 0.000 description 14
- 229940125396 insulin Drugs 0.000 description 14
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 12
- 210000001900 endoderm Anatomy 0.000 description 10
- 230000002255 enzymatic effect Effects 0.000 description 10
- 102000035195 Peptidases Human genes 0.000 description 9
- 108091005804 Peptidases Proteins 0.000 description 9
- 239000004365 Protease Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 241000699670 Mus sp. Species 0.000 description 8
- 238000004220 aggregation Methods 0.000 description 8
- 238000005538 encapsulation Methods 0.000 description 8
- 238000001727 in vivo Methods 0.000 description 8
- 230000009996 pancreatic endocrine effect Effects 0.000 description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 239000003814 drug Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 102000029816 Collagenase Human genes 0.000 description 6
- 108060005980 Collagenase Proteins 0.000 description 6
- 239000012980 RPMI-1640 medium Substances 0.000 description 6
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 6
- 239000002577 cryoprotective agent Substances 0.000 description 6
- 229950007919 egtazic acid Drugs 0.000 description 6
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 6
- 239000003112 inhibitor Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000011435 rock Substances 0.000 description 6
- 241000124008 Mammalia Species 0.000 description 5
- 229960002424 collagenase Drugs 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000000684 flow cytometry Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002243 precursor Substances 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 108010011459 Exenatide Proteins 0.000 description 4
- 102000051325 Glucagon Human genes 0.000 description 4
- 108060003199 Glucagon Proteins 0.000 description 4
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 4
- 210000002459 blastocyst Anatomy 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- JUFFVKRROAPVBI-PVOYSMBESA-N chembl1210015 Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)N[C@H]1[C@@H]([C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@]3(O[C@@H](C[C@H](O)[C@H](O)CO)[C@H](NC(C)=O)[C@@H](O)C3)C(O)=O)O2)O)[C@@H](CO)O1)NC(C)=O)C(=O)NCC(=O)NCC(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CO)C(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 JUFFVKRROAPVBI-PVOYSMBESA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 229960001519 exenatide Drugs 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 4
- 210000001654 germ layer Anatomy 0.000 description 4
- 229960004666 glucagon Drugs 0.000 description 4
- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 description 4
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 4
- 210000001778 pluripotent stem cell Anatomy 0.000 description 4
- 230000003248 secreting effect Effects 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 102000016387 Pancreatic elastase Human genes 0.000 description 3
- 108010067372 Pancreatic elastase Proteins 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 210000003981 ectoderm Anatomy 0.000 description 3
- 210000002308 embryonic cell Anatomy 0.000 description 3
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 230000010412 perfusion Effects 0.000 description 3
- 230000004043 responsiveness Effects 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 2
- DWJXYEABWRJFSP-XOBRGWDASA-N DAPT Chemical compound N([C@@H](C)C(=O)N[C@H](C(=O)OC(C)(C)C)C=1C=CC=CC=1)C(=O)CC1=CC(F)=CC(F)=C1 DWJXYEABWRJFSP-XOBRGWDASA-N 0.000 description 2
- 101800003838 Epidermal growth factor Proteins 0.000 description 2
- 102400001368 Epidermal growth factor Human genes 0.000 description 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 2
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 2
- 108090000385 Fibroblast growth factor 7 Proteins 0.000 description 2
- 102000003972 Fibroblast growth factor 7 Human genes 0.000 description 2
- 102000012004 Ghrelin Human genes 0.000 description 2
- 101800001586 Ghrelin Proteins 0.000 description 2
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 2
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 2
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 2
- 101000998020 Homo sapiens Keratin, type I cytoskeletal 18 Proteins 0.000 description 2
- 101000975496 Homo sapiens Keratin, type II cytoskeletal 8 Proteins 0.000 description 2
- 101710123134 Ice-binding protein Proteins 0.000 description 2
- 101710082837 Ice-structuring protein Proteins 0.000 description 2
- 206010022489 Insulin Resistance Diseases 0.000 description 2
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 2
- 102100033421 Keratin, type I cytoskeletal 18 Human genes 0.000 description 2
- 102100023972 Keratin, type II cytoskeletal 8 Human genes 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108010025020 Nerve Growth Factor Proteins 0.000 description 2
- 102000015336 Nerve Growth Factor Human genes 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 description 2
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 2
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 101710107540 Type-2 ice-structuring protein Proteins 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 230000011748 cell maturation Effects 0.000 description 2
- 230000008614 cellular interaction Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000004186 co-expression Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 102000038379 digestive enzymes Human genes 0.000 description 2
- 108091007734 digestive enzymes Proteins 0.000 description 2
- 238000011977 dual antiplatelet therapy Methods 0.000 description 2
- 229940116977 epidermal growth factor Drugs 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 210000004153 islets of langerhan Anatomy 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 210000003716 mesoderm Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012120 mounting media Substances 0.000 description 2
- 229940053128 nerve growth factor Drugs 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 1
- LUZOFMGZMUZSSK-LRDDRELGSA-N (-)-indolactam V Chemical compound C1[C@@H](CO)NC(=O)[C@H](C(C)C)N(C)C2=CC=CC3=C2C1=CN3 LUZOFMGZMUZSSK-LRDDRELGSA-N 0.000 description 1
- ABKJCDILEUEJSH-MHWRWJLKSA-N 2-[(e)-(6-carboxyhexanoylhydrazinylidene)methyl]benzoic acid Chemical compound OC(=O)CCCCCC(=O)N\N=C\C1=CC=CC=C1C(O)=O ABKJCDILEUEJSH-MHWRWJLKSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- OMKHWTRUYNAGFG-IEBDPFPHSA-N 3-deazaneplanocin a Chemical compound C1=NC=2C(N)=NC=CC=2N1[C@@H]1C=C(CO)[C@@H](O)[C@H]1O OMKHWTRUYNAGFG-IEBDPFPHSA-N 0.000 description 1
- 102000018918 Activin Receptors Human genes 0.000 description 1
- 108010052946 Activin Receptors Proteins 0.000 description 1
- 210000002237 B-cell of pancreatic islet Anatomy 0.000 description 1
- OBMZMSLWNNWEJA-XNCRXQDQSA-N C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 Chemical compound C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 OBMZMSLWNNWEJA-XNCRXQDQSA-N 0.000 description 1
- 102100039997 Gastric inhibitory polypeptide receptor Human genes 0.000 description 1
- 108010086246 Glucagon-Like Peptide-1 Receptor Proteins 0.000 description 1
- 101800000224 Glucagon-like peptide 1 Proteins 0.000 description 1
- DTHNMHAUYICORS-KTKZVXAJSA-N Glucagon-like peptide 1 Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1N=CNC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 DTHNMHAUYICORS-KTKZVXAJSA-N 0.000 description 1
- 102100032882 Glucagon-like peptide 1 receptor Human genes 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 101000886866 Homo sapiens Gastric inhibitory polypeptide receptor Proteins 0.000 description 1
- 101000984042 Homo sapiens Protein lin-28 homolog A Proteins 0.000 description 1
- 101000687905 Homo sapiens Transcription factor SOX-2 Proteins 0.000 description 1
- LUZOFMGZMUZSSK-UHFFFAOYSA-N Indolactam-V Natural products C1C(CO)NC(=O)C(C(C)C)N(C)C2=CC=CC3=C2C1=CN3 LUZOFMGZMUZSSK-UHFFFAOYSA-N 0.000 description 1
- 229940094910 Insulin receptor antagonist Drugs 0.000 description 1
- 102000036770 Islet Amyloid Polypeptide Human genes 0.000 description 1
- 108010041872 Islet Amyloid Polypeptide Proteins 0.000 description 1
- 101710144033 Pancreas/duodenum homeobox protein 1 Proteins 0.000 description 1
- 102000052651 Pancreatic hormone Human genes 0.000 description 1
- 101800001268 Pancreatic hormone Proteins 0.000 description 1
- 101710176384 Peptide 1 Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 102100040918 Pro-glucagon Human genes 0.000 description 1
- 102100025460 Protein lin-28 homolog A Human genes 0.000 description 1
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 description 1
- 238000011579 SCID mouse model Methods 0.000 description 1
- UAKWLVYMKBWHMX-UHFFFAOYSA-N SU4312 Chemical compound C1=CC(N(C)C)=CC=C1C=C1C2=CC=CC=C2NC1=O UAKWLVYMKBWHMX-UHFFFAOYSA-N 0.000 description 1
- 206010043276 Teratoma Diseases 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 102100024270 Transcription factor SOX-2 Human genes 0.000 description 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 1
- 102100030742 Transforming growth factor beta-1 proprotein Human genes 0.000 description 1
- FALILNHGILFDLC-UHFFFAOYSA-N [2-hydroxy-4-(4-morpholinyl)phenyl]-phenylmethanone Chemical compound OC1=CC(N2CCOCC2)=CC=C1C(=O)C1=CC=CC=C1 FALILNHGILFDLC-UHFFFAOYSA-N 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
- PLOPBXQQPZYQFA-AXPWDRQUSA-N amlintide Chemical compound C([C@@H](C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H]1NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CCCCN)CSSC1)[C@@H](C)O)C(C)C)C1=CC=CC=C1 PLOPBXQQPZYQFA-AXPWDRQUSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- -1 difluorophenylacetyl Chemical group 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000000081 effect on glucose Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000004205 endocrine pancreatic function Effects 0.000 description 1
- 238000004146 energy storage Methods 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 210000000646 extraembryonic cell Anatomy 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 238000011990 functional testing Methods 0.000 description 1
- 230000004190 glucose uptake Effects 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000002991 immunohistochemical analysis Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 238000007410 oral glucose tolerance test Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000004025 pancreas hormone Substances 0.000 description 1
- 210000003577 pancreatic endocrine progenitor Anatomy 0.000 description 1
- 229940032957 pancreatic hormone Drugs 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000013094 purity test Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000012372 quality testing Methods 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 229930002330 retinoic acid Natural products 0.000 description 1
- 239000003590 rho kinase inhibitor Substances 0.000 description 1
- 238000009781 safety test method Methods 0.000 description 1
- 210000002955 secretory cell Anatomy 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 230000000920 spermatogeneic effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 229960001727 tretinoin Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
- C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/39—Pancreas; Islets of Langerhans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2523/00—Culture process characterised by temperature
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Diabetes (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
- Nutrition Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Emergency Medicine (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP17190412 | 2017-09-11 | ||
EP17190412.1 | 2017-09-11 | ||
PCT/EP2018/074390 WO2019048690A1 (en) | 2017-09-11 | 2018-09-11 | ENRICHMENT OF CELLS COEXPRESSING NKX6.1 AND PEPTIDE C, IN VITRO DERIVATIVES FROM STEM CELLS |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2020532978A JP2020532978A (ja) | 2020-11-19 |
JP7389020B2 true JP7389020B2 (ja) | 2023-11-29 |
Family
ID=59846503
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020512652A Active JP7389020B2 (ja) | 2017-09-11 | 2018-09-11 | 幹細胞からインビトロで誘導されたnkx6.1およびc-ペプチド共発現細胞の濃縮 |
Country Status (16)
Country | Link |
---|---|
US (1) | US20200199540A1 (pt) |
EP (1) | EP3681992A1 (pt) |
JP (1) | JP7389020B2 (pt) |
KR (1) | KR20200051664A (pt) |
CN (1) | CN111108190B (pt) |
AU (1) | AU2018330499A1 (pt) |
BR (1) | BR112020004428A2 (pt) |
CA (1) | CA3074910A1 (pt) |
CO (1) | CO2020003122A2 (pt) |
IL (1) | IL272734A (pt) |
MA (1) | MA50279A (pt) |
MX (1) | MX2020002421A (pt) |
RU (1) | RU2020111055A (pt) |
SA (1) | SA520411466B1 (pt) |
SG (1) | SG11202001906PA (pt) |
WO (1) | WO2019048690A1 (pt) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20220177849A1 (en) | 2019-04-08 | 2022-06-09 | Novo Nordisk A/S | Generation of pancreatic endoderm from stem cell derived definitive endoderm |
EP4061929A1 (en) | 2019-11-22 | 2022-09-28 | Novo Nordisk A/S | Spin-aggregated neural microspheres and the application thereof |
WO2023203208A1 (en) | 2022-04-21 | 2023-10-26 | Evotec International Gmbh | New cell populations and means and methods for their differentiation and preservation |
WO2024008810A1 (en) | 2022-07-06 | 2024-01-11 | Novo Nordisk A/S | Differentiation of stem cells to pancreatic endocrine cells |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016504037A (ja) | 2012-12-31 | 2016-02-12 | ヤンセン バイオテツク,インコーポレーテツド | 膵内分泌細胞への分化のためのヒト多能性細胞の懸濁及びクラスタリング |
JP2016513972A (ja) | 2013-03-14 | 2016-05-19 | ヴィアサイト インコーポレイテッド | 多能性幹細胞の膵臓内胚葉細胞(PEC)および内分泌細胞へのinvitro分化 |
WO2017144695A1 (en) | 2016-02-24 | 2017-08-31 | Novo Nordisk A/S | Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2018638C (en) * | 1990-06-08 | 2002-10-01 | Wah Jun Tze | Banking of pancreatic endocrine cells for transplantation |
JP2005512593A (ja) | 2001-12-28 | 2005-05-12 | セルアーティス アーベー | 多能性のヒト胚盤胞由来幹細胞株の樹立方法 |
GB2445338A (en) | 2005-10-07 | 2008-07-02 | Cellartis Ab | A method for obtaining a xeno-free HBS cell line |
US7695965B2 (en) * | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
US9080145B2 (en) * | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
EP2356227B1 (en) * | 2008-11-14 | 2018-03-28 | Viacyte, Inc. | Encapsulation of pancreatic cells derived from human pluripotent stem cells |
RU2607380C2 (ru) * | 2010-03-01 | 2017-01-10 | Янссен Байотек, Инк. | Способы очистки клеток, производных от плюрипотентных стволовых клеток |
CN108220224A (zh) | 2011-06-21 | 2018-06-29 | 诺沃—诺迪斯克有限公司 | 自多潜能干细胞有效诱导定形内胚层 |
EP2893000B1 (en) | 2012-09-03 | 2019-04-10 | Novo Nordisk A/S | Generation of pancreatic endoderm from pluripotent stem cells using small molecules |
WO2014138671A2 (en) * | 2013-03-08 | 2014-09-12 | Viacyte, Inc. | Cryopreservation, hibernation and room temperature storage of encapulated pancreatic endoderm cell aggregates |
CN105683361B (zh) * | 2013-08-30 | 2021-04-02 | 诺和诺德股份有限公司 | 使用小分子从人多能干细胞产生内分泌祖细胞 |
JP2017112835A (ja) * | 2014-04-17 | 2017-06-29 | 東京エレクトロン株式会社 | 多能性幹細胞の凍結保存方法および凍結保存システム |
WO2017094001A1 (en) * | 2015-11-30 | 2017-06-08 | Kadimastem Ltd | Methods for differentiating and purifying pancreatic endocrine cells |
-
2018
- 2018-09-11 BR BR112020004428-8A patent/BR112020004428A2/pt unknown
- 2018-09-11 MX MX2020002421A patent/MX2020002421A/es unknown
- 2018-09-11 MA MA050279A patent/MA50279A/fr unknown
- 2018-09-11 AU AU2018330499A patent/AU2018330499A1/en active Pending
- 2018-09-11 CA CA3074910A patent/CA3074910A1/en active Pending
- 2018-09-11 SG SG11202001906PA patent/SG11202001906PA/en unknown
- 2018-09-11 WO PCT/EP2018/074390 patent/WO2019048690A1/en active Application Filing
- 2018-09-11 KR KR1020207008866A patent/KR20200051664A/ko not_active Application Discontinuation
- 2018-09-11 US US16/645,840 patent/US20200199540A1/en active Pending
- 2018-09-11 RU RU2020111055A patent/RU2020111055A/ru unknown
- 2018-09-11 EP EP18769975.6A patent/EP3681992A1/en active Pending
- 2018-09-11 JP JP2020512652A patent/JP7389020B2/ja active Active
- 2018-09-11 CN CN201880058738.XA patent/CN111108190B/zh active Active
-
2020
- 2020-02-18 IL IL272734A patent/IL272734A/en unknown
- 2020-03-03 SA SA520411466A patent/SA520411466B1/ar unknown
- 2020-03-16 CO CONC2020/0003122A patent/CO2020003122A2/es unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016504037A (ja) | 2012-12-31 | 2016-02-12 | ヤンセン バイオテツク,インコーポレーテツド | 膵内分泌細胞への分化のためのヒト多能性細胞の懸濁及びクラスタリング |
JP2016513972A (ja) | 2013-03-14 | 2016-05-19 | ヴィアサイト インコーポレイテッド | 多能性幹細胞の膵臓内胚葉細胞(PEC)および内分泌細胞へのinvitro分化 |
WO2017144695A1 (en) | 2016-02-24 | 2017-08-31 | Novo Nordisk A/S | Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors |
Non-Patent Citations (1)
Title |
---|
Stem Cells Translational Medicine,2015年,Vol.4,pp.1214-1222 |
Also Published As
Publication number | Publication date |
---|---|
MX2020002421A (es) | 2020-07-13 |
CO2020003122A2 (es) | 2020-06-19 |
US20200199540A1 (en) | 2020-06-25 |
CA3074910A1 (en) | 2019-03-14 |
RU2020111055A (ru) | 2021-09-17 |
CN111108190B (zh) | 2024-06-21 |
EP3681992A1 (en) | 2020-07-22 |
MA50279A (fr) | 2020-07-22 |
WO2019048690A1 (en) | 2019-03-14 |
SA520411466B1 (ar) | 2024-03-10 |
JP2020532978A (ja) | 2020-11-19 |
BR112020004428A2 (pt) | 2020-09-08 |
RU2020111055A3 (pt) | 2022-04-22 |
IL272734A (en) | 2020-04-30 |
CN111108190A (zh) | 2020-05-05 |
SG11202001906PA (en) | 2020-04-29 |
AU2018330499A1 (en) | 2020-04-09 |
KR20200051664A (ko) | 2020-05-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP7389020B2 (ja) | 幹細胞からインビトロで誘導されたnkx6.1およびc-ペプチド共発現細胞の濃縮 | |
Chen et al. | Current progress in stem cell therapy for type 1 diabetes mellitus | |
EP3420073B1 (en) | Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors | |
EP2981605B1 (en) | Methods and compositions for culturing endoderm progenitor cells in suspension | |
Fiorina et al. | Immunological applications of stem cells in type 1 diabetes | |
CA2666789C (en) | Embryonic-like stem cells derived from adult human peripheral blood and methods of use | |
Gao et al. | In vitro cultivation of islet-like cell clusters from human umbilical cord blood-derived mesenchymal stem cells | |
AU2006203990B2 (en) | Regeneration of pancreatic islets by amniotic fluid stem cell therapy | |
JP6647864B2 (ja) | 赤血球の産生 | |
JP2012040025A (ja) | ヒト胚幹細胞由来の膵島細胞 | |
Santana et al. | Insulin‐producing cells derived from stem cells: recent progress and future directions | |
Márquez-Aguirre et al. | Development of the endocrine pancreas and novel strategies for β-cell mass restoration and diabetes therapy | |
JP2024045311A (ja) | 島細胞製造組成物および使用方法 | |
Rattananinsruang et al. | Establishment of insulin-producing cells from human embryonic stem cells underhypoxic condition for cell based therapy | |
Tsao et al. | Establishment of propagable epithelial cell lines from normal adult rat pancreas | |
JP2024045609A (ja) | RNAでの幹細胞分化による膵臓β細胞の誘導 | |
Cao et al. | Alleviation of streptozotocin-induced diabetes in nude mice by stem cells derived from human first trimester umbilical cord | |
Roche et al. | Generation of new islets from stem cells | |
Şuşman et al. | Human placenta–stem cell source for obtaining pancreatic progenitors | |
McGaugh | Elucidating the Role of FGF and RA Signaling During Pancreatic Differentiation Using hPSC | |
Kaufman et al. | Embryonicmaintainedlines, of yet human retain stem indefinitely embryonic (ES) their cells ability in are stem culture pluripotent to cells form as provides undifferentiated any cells cell a that new type. can model The cell | |
Espinha | Bioprocess engineering of induced pluripotent stem cells for application in cell therapy and pre-clinical research | |
Odorico et al. | Modeling islet development through embryonic stem cell differentiation | |
Shahjalal | Pancreatic differentiation of human iPS cells using a defined and completely xeno-free culture system | |
Lumelsky | Pancreatic Differentiation of Pluripotent Stem Cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20200513 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20200515 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20210816 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20220623 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20220711 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20221005 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20221206 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230106 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230403 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20230622 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230823 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20231023 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20231116 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7389020 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |