JP7364333B2 - 変異体ポア - Google Patents

変異体ポア Download PDF

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Publication number
JP7364333B2
JP7364333B2 JP2018552853A JP2018552853A JP7364333B2 JP 7364333 B2 JP7364333 B2 JP 7364333B2 JP 2018552853 A JP2018552853 A JP 2018552853A JP 2018552853 A JP2018552853 A JP 2018552853A JP 7364333 B2 JP7364333 B2 JP 7364333B2
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Japan
Prior art keywords
pore
variant
monomer
seq
lysenin
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Japanese (ja)
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JP2019520035A5 (enExample
JP2019520035A (ja
Inventor
ジャヤシンゲ，ラクマル
ブルース，マーク
マクニール，ルーク
イクバール ネイサニ，ラミッツ
ラジ シング，プラティク
ロジャー ウッド，ネイル
ロバート ヤング，ステファン
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オックスフォード ナノポール テクノロジーズ ピーエルシー
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Priority claimed from GBGB1608274.5A external-priority patent/GB201608274D0/en
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Publication of JP2019520035A publication Critical patent/JP2019520035A/ja
Publication of JP2019520035A5 publication Critical patent/JP2019520035A5/ja
Priority to JP2022041806A priority Critical patent/JP7555987B2/ja
Application granted granted Critical
Publication of JP7364333B2 publication Critical patent/JP7364333B2/ja
Priority to JP2024095022A priority patent/JP2024138262A/ja
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nanotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Peptides Or Proteins (AREA)
JP2018552853A 2016-04-06 2017-04-06 変異体ポア Active JP7364333B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022041806A JP7555987B2 (ja) 2016-04-06 2022-03-16 変異体ポア
JP2024095022A JP2024138262A (ja) 2016-04-06 2024-06-12 変異体ポア

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1605899.2 2016-04-06
GB201605899 2016-04-06
GBGB1608274.5A GB201608274D0 (en) 2016-05-11 2016-05-11 Mutant pore
GB1608274.5 2016-05-11
PCT/GB2017/050961 WO2017174990A1 (en) 2016-04-06 2017-04-06 Mutant pore

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022041806A Division JP7555987B2 (ja) 2016-04-06 2022-03-16 変異体ポア

Publications (3)

Publication Number Publication Date
JP2019520035A JP2019520035A (ja) 2019-07-18
JP2019520035A5 JP2019520035A5 (enExample) 2020-05-14
JP7364333B2 true JP7364333B2 (ja) 2023-10-18

Family

ID=58672620

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2018552853A Active JP7364333B2 (ja) 2016-04-06 2017-04-06 変異体ポア
JP2022041806A Active JP7555987B2 (ja) 2016-04-06 2022-03-16 変異体ポア
JP2024095022A Pending JP2024138262A (ja) 2016-04-06 2024-06-12 変異体ポア

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2022041806A Active JP7555987B2 (ja) 2016-04-06 2022-03-16 変異体ポア
JP2024095022A Pending JP2024138262A (ja) 2016-04-06 2024-06-12 変異体ポア

Country Status (8)

Country Link
US (3) US11104709B2 (enExample)
EP (3) EP4122949B1 (enExample)
JP (3) JP7364333B2 (enExample)
KR (1) KR102472805B1 (enExample)
CN (6) CN116514944A (enExample)
AU (1) AU2017246690B2 (enExample)
CA (2) CA3212147A1 (enExample)
WO (1) WO2017174990A1 (enExample)

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EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
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EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
JP7364333B2 (ja) * 2016-04-06 2023-10-18 オックスフォード ナノポール テクノロジーズ ピーエルシー 変異体ポア
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB202015993D0 (en) * 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
CN113896776B (zh) * 2021-10-12 2024-02-06 成都齐碳科技有限公司 孔蛋白单体的突变体、蛋白孔及其应用
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores

Citations (1)

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Publication number Priority date Publication date Assignee Title
JP2015514128A (ja) 2012-04-10 2015-05-18 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア

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