JP7358347B2 - 組み換えＥ．ｃｏｌｉアスパラギナーゼの製造のための方法 - Google Patents

組み換えＥ．ｃｏｌｉアスパラギナーゼの製造のための方法 Download PDF

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JP7358347B2
JP7358347B2 JP2020523340A JP2020523340A JP7358347B2 JP 7358347 B2 JP7358347 B2 JP 7358347B2 JP 2020523340 A JP2020523340 A JP 2020523340A JP 2020523340 A JP2020523340 A JP 2020523340A JP 7358347 B2 JP7358347 B2 JP 7358347B2
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asparaginase
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ラッセル・ジェイ・コールマン
トーベン・ブルック
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フェネックス インク．
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01001—Asparaginase (3.5.1.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/39—Pseudomonas fluorescens
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2020523340A 2017-10-27 2018-10-17 組み換えＥ．ｃｏｌｉアスパラギナーゼの製造のための方法 Active JP7358347B2 (ja)

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Application Number Priority Date Filing Date Title
US201762578302P 2017-10-27 2017-10-27
US62/578,302 2017-10-27
PCT/US2018/056375 WO2019083794A1 (en) 2017-10-27 2018-10-17 PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI

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JP2021500892A JP2021500892A (ja) 2021-01-14
JP2021500892A5 JP2021500892A5 (https=) 2021-11-18
JP7358347B2 true JP7358347B2 (ja) 2023-10-10

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US (2) US10662433B2 (https=)
EP (1) EP3701036A4 (https=)
JP (1) JP7358347B2 (https=)
CN (1) CN111278979B (https=)
AU (1) AU2018354068C1 (https=)
SG (1) SG11202003614WA (https=)
WO (1) WO2019083794A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019083794A1 (en) * 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI
JP7784804B2 (ja) * 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
GB201912020D0 (en) * 2019-08-21 2019-10-02 Porton Biopharma Ltd Therapeutic Conjugate
JP2022553399A (ja) * 2019-10-25 2022-12-22 ジャズ ファーマシューティカルズ アイルランド リミテッド 組換えｌ－アスパラギナーゼ
CN110904085B (zh) * 2019-12-26 2021-04-16 常州千红生化制药股份有限公司 一种发酵法制备门冬酰胺酶的方法
CN118440925B (zh) * 2023-03-14 2024-12-24 医图生科(苏州)生命科学技术有限公司 一种低免疫原性门冬酰胺酶突变体及其制备方法和应用
WO2025157811A1 (fr) * 2024-01-23 2025-07-31 Asfalia Biologics Promoteurs inductibles par des molecules pharmacologiques

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008507294A (ja) 2004-07-26 2008-03-13 ダウ グローバル テクノロジーズ インコーポレイティド 菌株遺伝子操作による改善されたタンパク質発現のための方法
JP2009542219A (ja) 2006-06-30 2009-12-03 エンゾン ファーマスーティカルズ インコーポレイテッド Ｌ−アスパラギナーゼｉｉを産生する組換え宿主
JP2010517532A (ja) 2007-01-31 2010-05-27 ダウ グローバル テクノロジーズ インコーポレイティド 発現上昇のための細菌リーダー配列
JP2010524506A (ja) 2007-04-27 2010-07-22 ダウ グローバル テクノロジーズ インコーポレイティド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および／または質が改善されている特定の株を同定する方法
JP2013529064A (ja) 2010-03-30 2013-07-18 フェニックス インク． 組み換え型毒素タンパク質の高レベルの発現

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
GB8519753D0 (en) * 1985-08-06 1985-09-11 Health Lab Service Board Production of 1-asparaginase
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
GB9017002D0 (en) 1990-08-02 1990-09-19 Health Lab Service Board Improved method for the purification of erwina l-asparaginase
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
CA2545610C (en) 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Auxotrophic pseudomonas fluorescens bacteria for recombinant protein expression
AU2004317306B2 (en) 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
EP2021489A2 (en) 2006-05-30 2009-02-11 Dow Global Technologies Inc. Codon optimization method
WO2008128975A1 (en) 2007-04-20 2008-10-30 Dsm Ip Assets B.V. Novel asparaginases and uses thereof
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2011003633A1 (en) 2009-07-06 2011-01-13 Alize Pharma Ii Pegylated l-asparaginase
CN103060299B (zh) * 2011-10-19 2017-05-03 北京安百胜生物科技有限公司 活性提高的l‑天冬酰胺酶变体
BR102014000585B1 (pt) 2014-01-10 2021-01-12 Coppe/Ufrj-Instituto Alberto Luiz Coimbra De Pós-Graduação l-asparaginase recombinante de zymomonas
KR102092225B1 (ko) 2014-04-30 2020-03-23 주식회사 엘지화학 고효율 분비능을 가지는 단백질 분비 인자 및 이를 포함하는 발현 벡터
JP6817939B2 (ja) 2014-12-01 2021-01-20 フェネックス インク． ペプチド産生のための融合パートナー
JP7784804B2 (ja) * 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
WO2019083794A1 (en) * 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008507294A (ja) 2004-07-26 2008-03-13 ダウ グローバル テクノロジーズ インコーポレイティド 菌株遺伝子操作による改善されたタンパク質発現のための方法
JP2009542219A (ja) 2006-06-30 2009-12-03 エンゾン ファーマスーティカルズ インコーポレイテッド Ｌ−アスパラギナーゼｉｉを産生する組換え宿主
JP2010517532A (ja) 2007-01-31 2010-05-27 ダウ グローバル テクノロジーズ インコーポレイティド 発現上昇のための細菌リーダー配列
JP2010524506A (ja) 2007-04-27 2010-07-22 ダウ グローバル テクノロジーズ インコーポレイティド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および／または質が改善されている特定の株を同定する方法
JP2013529064A (ja) 2010-03-30 2013-07-18 フェニックス インク． 組み換え型毒素タンパク質の高レベルの発現

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FEMS Microbiology Letters，1999年，vol.178，p.327-335
Protein expression and purification，2012年，Vol.81，p.157-165

Also Published As

Publication number Publication date
EP3701036A4 (en) 2021-09-01
CN111278979B (zh) 2024-05-31
US11046964B2 (en) 2021-06-29
AU2018354068A1 (en) 2020-06-11
SG11202003614WA (en) 2020-05-28
AU2018354068B9 (en) 2022-11-24
EP3701036A1 (en) 2020-09-02
AU2018354068B2 (en) 2022-11-10
JP2021500892A (ja) 2021-01-14
CN111278979A (zh) 2020-06-12
AU2018354068C1 (en) 2023-03-23
US10662433B2 (en) 2020-05-26
US20190127743A1 (en) 2019-05-02
WO2019083794A1 (en) 2019-05-02
US20200248193A1 (en) 2020-08-06

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