JP7325925B2 - Production method of the mother tree - Google Patents

Description

TECHNICAL FIELD The present invention relates to a method for producing a mother tree for harvesting.

A scion garden is a managed garden in which mother trees are planted in rows and cultivated for the purpose of mass-producing cuttings for clonal propagation of cuttings of forest trees and fruit trees. In order to promote the growth of the mother tree and obtain a large number of cuttings, it is known to increase the branch length and the number of branches by applying fertilizer until just before the ear collection.

On the other hand, the way the mother tree is harvested varies depending on the species and region, but it is mainly tailored to low base (round cutting/flat cutting), middle base (round cutting/flat cutting), and high base (round cutting/cylindrical type). It is known that sprouts can be obtained by In particular, cedar harvesting orchards are mainly built on high ground, and in warm regions such as Kyushu, they are cut in a circle, and in cold regions such as Tohoku, they are made cylindrical to avoid the effects of accumulated snow. Sprouting shoots at each ear collection are characterized by being longer (usually 30 to 40 cm) in round cuttings and shorter (usually 5 to 25 cm) in cylindrical preparations.

Nobuaki Takeda "- Series Seed (Spike) Orchard Management (4)-Management of the Spike Orchard" Forest Tree Breeding Information No. 1 (2008) Noboru Ota "Actual Cutting Business in the Tohoku Region (2)" Forest Tree Breeding in Tohoku Edited by Tohoku Forest Tree Breeding Center No. 34 (1971)

However, if the mother tree is fertilized until just before ear collection, the growth of the branches of the mother tree can be promoted, while the shoot apical meristem grows by repeating cell division, which softens the apical bud. Therefore, in the case of forest seedlings, in which seedlings are produced by apical cuttings, there is a problem that scions suitable for cuttings cannot be obtained.

On the other hand, for the methods of Non-Patent Documents 1 and 2, the reason why round cutting is performed in a warm region is that it is mainstream to insert cuttings of 30 to 40 cm as they are, but they are collected from one mother tree. There was a limit to the number of cuttings that could produce ears. In addition, even if a 30 to 40 cm round-cut rough ear is subdivided into 10 to 20 cm, the number of cuttings that can be used as cuttings is limited due to the problem of branching. On the other hand, a cylindrical mother tree can produce a relatively large number of sprouts that are difficult to branch out, but the branch length is shorter than that of the close-cropped mother tree, and rooting is low due to the smaller leaf area.

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for producing a mother tree from which a large number of cuttings with a good rooting rate can be obtained.

The present invention provides the following [1] to [7].
[1] The mother tree should be fertilized at least once in the year before the scheduled date of ear collection, and the period from the last fertilization to the insertion before the scheduled date of ear collection should be 2 months or more and 9 months. A method of producing a scion mother tree, comprising:
[2] The production method according to [1], wherein the number of times of fertilization is two or more, and the interval between each fertilization is at least one month.
[3] The production method according to [1] or [2], wherein the scheduled ear collection date is from October to April.
[4] The production method according to any one of [1] to [3], further comprising making the shape of the mother tree for harvesting into a cylindrical shape on a hill.
[5] The scion mother tree according to any one of [1] to [4], wherein the scion mother tree is a plant species selected from plants of the genus Cedar, plants of the genus Pinus, plants of the genus Hinoki, and plants of the genus Eucalyptus. production method.
[6] To obtain cuttings by collecting ears from the mother tree produced by the production method according to any one of [1] to [5] on or near the scheduled date for collecting ears, and rooting the cuttings. and raising the rooted cuttings.
[7] The method according to [6], wherein the size of the collected cuttings is 40 cm or less.

ADVANTAGE OF THE INVENTION According to this invention, the production method of the cutting mother tree which can obtain a large number of cuttings with a favorable rooting rate is provided. A large number of cutting seedlings can be obtained by rooting and growing the cuttings obtained from such a mother tree.

[Collecting mother tree]
The plant species of the mother tree for collecting ears is not particularly limited, and may be either a woody plant or a herbaceous plant. Examples of woody plants include Cryptomeria plants (Cryptomeria japonica, etc.), Chamaecyparis plants (Chamaecyparis obtusa, etc.), Pinaceae plants (Pinus plants) (Pinus thunbergii, etc.), Larix plants (Larix kaempferi, Larix gmelinii, etc.), Abies plants (Abies sachalinensis, etc.), etc.), Eucalyptus ( Eucalyptus) plants, Prunus plants (Prunus spp., Prunus mume, Prunus tomentosa, etc.), Mangifera plants (Mangifera indica, etc.), Acacia genus ( Acacia) plants, Myrica plants, Quercus plants (Quercus acutissima), Vitis plants, Malus plants, Rosa plants, Camellia ( Camellia plants (such as Camellia sinensis), Jacaranda plants (such as Jacaranda mimosifolia), Persea plants (such as avocado (Persea americana)), Pyrus plants (pear (Pyrus serotina Rehder, Pyrus pyrifolia), etc.), sandalwood genus (Santalum) plants (sandalwood (Santalum album), etc.), cedar plants, cypress plants, Pinaceae plants (Pine plants, Larix plants, plants of the genus Fir, etc.), plants of the genus Eucalyptus, plants of the genus Camellia, plants of the genus Mango, and plants of the genus Alligator pear are preferred, and plants of the genus Cedar, cypress, pine, larch, fir, and eucalyptus are preferred. More preferred are plants belonging to the genus Cedar, plants belonging to the genus Pinus, plants belonging to the genus Hinoki, and plants belonging to the genus Eucalyptus. Examples of plant species include cedar, cypress, pine (black pine, larch, pine, Sakhalin fir, etc.), eucalyptus, cherry, mango, avocado, acacia, bayberry, sawtooth oak, grape, apple, rose, camellia, tea, plum, todomatsu, Jacaranda is mentioned.

The mother tree from which the ear is collected may be an individual propagated by asexual reproduction such as a cutting or a graft, or an individual propagated from a seed such as a seedling. The age of the mother tree from which the ears are collected is not particularly limited, but is preferably 10 years or less. Although the lower limit is not particularly limited, it is usually one year or longer.

[Fertilization frequency]
In the present invention, the number of times of fertilizing the mother tree for ear collection is at least one year before the scheduled ear collection date (if ear collection was performed in the previous year, usually between the previous year's ear collection and the scheduled ear collection date). times, preferably two times or more. Although the upper limit is not particularly limited, it is usually 5 times or less, preferably 4 times or less, and more preferably 3 times or less. As a result, the growth of the mother tree from which the ears are collected can be promoted. When fertilization is performed twice or more, the interval between each fertilization is preferably at least one month, preferably 1.5 months or more. Although the upper limit is not particularly limited, it is usually within 3 months, preferably within 2.5 months.

[Timing of final fertilization]
In the present invention, the period from the last application of fertilizer to the mother tree before the scheduled date of harvesting until the cutting is 2 months or more, preferably 3 months or more, and more preferably 4 months or more. As a result, a sufficient fertilization stop period can be taken, so that the growth of the apical bud of the mother tree for collection can be stopped, and a large number of hard ears suitable for cuttings can be obtained. Seedlings obtained from cuttings may also grow better after cuttings (eg, grow straighter). The upper limit of the period from the last fertilization to planting is preferably 9 months or less, 8 months or less, or 7 months or less, more preferably 6 months or less, and even more preferably 5 months or less. As a result, a sufficient fertilization period can be taken, and the growth of the mother tree for collection can be promoted.

[Fertilizer given to the mother tree]
In the present invention, the fertilizer to be applied to the mother tree for harvesting is not particularly limited, but a fast-acting fertilizer is preferable, an inorganic fertilizer or an organic fertilizer is more preferable, and a chemical fertilizer is even more preferable.

The ingredients contained in the fertilizer are not particularly limited, but include, for example, inorganic ingredients, silver ions, antioxidants, carbon sources, vitamins, amino acids, ingredients that can be sources of nutrients for plants such as plant hormones. The form of the fertilizer is not particularly limited, and may be solid (eg, powder, granules) or liquid (eg, liquid fertilizer).

Examples of inorganic components include elements such as nitrogen, phosphorus, potassium, sulfur, calcium, magnesium, iron, manganese, zinc, boron, molybdenum, chlorine, iodine, and cobalt, and inorganic salts containing these. Examples of the inorganic salt include potassium nitrate, ammonium nitrate, ammonium chloride, sodium nitrate, potassium monohydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, potassium sulfate, ammonium sulfate, magnesium sulfate, ferrous sulfate, ferric sulfate, Manganese sulfate, zinc sulfate, copper sulfate, sodium sulfate, calcium chloride, magnesium chloride, boric acid, molybdenum trioxide, sodium molybdate, potassium iodide, cobalt chloride, and hydrates thereof.

Antioxidants include, for example, ascorbic acid and sulfites, with ascorbic acid being preferred. Ascorbic acid has low persistence in culture media, and thus can suppress environmental pollution.

Examples of carbon sources include compounds such as carbohydrates such as sucrose and derivatives thereof; organic acids such as fatty acids; primary alcohols such as ethanol.

Examples of vitamins include biotin, thiamine (vitamin B1), pyridoxine (vitamin B4), pyridoxal, pyridoxamine, calcium pantothenate, inositol, nicotinic acid, nicotinamide and/or riboflavin (vitamin B2).

Amino acids include, for example, glycine, alanine, glutamic acid, cysteine, phenylalanine and/or lysine.

[Fertilization of the mother tree for collecting ears]
The fertilization method is not particularly limited, and fertilization conditions suitable for the fertilizer to be used may be set. Examples thereof include a method of spreading, coating, or spraying an appropriate amount of fertilizer on the support of the scion mother tree and/or the scion mother tree. The fertilization method may be different for each fertilization frequency, or may be the same.

[Scheduled ear harvesting date]
The expected harvesting date is not particularly limited, but examples include months when the average temperature is 20° C. or less, preferably October to April, more preferably November to March. When the mother tree for harvesting ears is produced in Japan, it is more preferable to set the scheduled harvesting date within the above range. The actual ear collection date may be a day between 10 days before and after the scheduled ear collection date, but is preferably the same day as the scheduled ear collection date.

[Tree shape, pruning]
In the present invention, it is preferable that the tree form of the mother tree for harvesting on the scheduled date of harvesting is a cylindrical shape on a hill. As a result, a large number of cuttings can be obtained from one mother tree from which the cuttings have been collected, and the rate of rooting from the cuttings can also be increased. The tree shape means the height of the rootstock and the shape of the tree trunk, and the hill-cylindrical shape means that the tree height is high and the trunk is cylindrical. The size of the hill cylindrical shape is not particularly limited, and it varies depending on the plant species and the age of the tree, and although it is difficult to specify uniformly, an example is as follows. The height of the rootstock is preferably 1.2-2.3 m, more preferably 1.5-2 m. The crown size is preferably 0.4 to 0.9 m in diameter, more preferably 0.5 to 0.8 m.

The timing of tailoring is not particularly limited, but it is usually before the scheduled ear collection date (if ear collection was performed in the previous year, usually after the previous year's ear collection date), and preferably before the final fertilization. The period before the first fertilization is preferable within one year before the scheduled harvesting date.

Examples of methods for making a tree mold into a cylindrical shape on a hill include trunk cutting and branch pruning. The height of the tree can be adjusted by cutting the trunk. Pruning of branches can also produce more sprouts. Trunk cutting is preferably carried out so that the tree height after cutting is usually 2.5 m or less, preferably 2 m or less. Although the lower limit is not particularly limited, it is preferably 0.9 m or more, more preferably 1 m or more. Branches to be pruned are preferably branches expected to generate sprouting branches, and such branches include, for example, green branches (current-year branches) and mature branches (branches grown before the previous year). The branch size after pruning is preferably less than 20 cm, more preferably 17 cm or less, and even more preferably 15 cm or less. Although the lower limit is not particularly limited, it is preferably 3 cm or more, more preferably 4 cm or more, and even more preferably 5 cm or more.

The method of planting the mother tree for collecting ears is not particularly limited, but when growing a plurality of mother trees for collecting ears, it is preferable to plant them in rows. The planting interval is preferably 1 to 2 m x 1 to 2 m. As a result, it is possible to easily tailor the tree form of the mother tree from which the ears are collected.

The support for growing the harvested mother tree may be any material that is commonly used for growing plants, and examples thereof include natural soil such as sand and soil (eg, Akadama soil). Other examples of supports include artificial soil such as rice husk charcoal, coconut fiber, vermiculite, perlite, peat moss, glass beads; porous moldings such as foamed phenolic resin, rock wool; ), and at least one selected from these may be used instead of natural soil or used together with natural soil. The support may be stored in a container. As the container, a conventional container may be used.

[Other conditions for production of the mother tree for harvesting]
Other conditions (eg, temperature, humidity, and light) for the production of the mother tree for harvesting can be appropriately set depending on the plant species, and may be natural conditions or may be artificially controlled. The place of growth is also not particularly limited, and may be either a closed space (eg, inside a vinyl house, a room with artificial sunlight, a greenhouse, indoors) or an open space (eg, outdoors).

[Cutting]
In the present invention, cuttings are obtained from the mother tree from which the ears are collected. The cuttings to be collected may be at least a part of the plant body of the mother tree from which the ears are collected. For example, branches such as green branches and mature branches; exemplified. Cuttings of woody plants generally preferably contain at least green shoots or mature shoots. Cuttings of herbaceous plants are generally preferred to contain leaves or shoots. On the other hand, the cuttings preferably contain at least shoots. This can facilitate adventitious root formation. Shoot refers to a tissue capable of rooting, for example, branches, stems, sprouts, apical buds, axillary buds, adventitious buds, leaves, cotyledons, hypocotyls, adventitious embryos, shoot primordia, and those derived from these specific examples. (JP-A-8-228621), and germination is preferred. In the present invention, branches containing at least one shoot (preferably sprout) can be efficiently obtained.

The size of the cuttings to be collected is preferably 40 cm or less, more preferably 35 cm or less, still more preferably 30 cm or less, and even more preferably 25 cm or less. There is no particular lower limit, but it is usually 1 cm or more or 2 cm or more, preferably 3 cm or more, more preferably 4 cm or more, and still more preferably 5 cm or more. In the present invention, even small cuttings can exhibit a good rooting rate.

[Rooting from cuttings]
In the present invention, rooting from cuttings may be carried out by a conventional method. For example, there is a method of inserting cuttings into a support and rooting them. The support may contain additives used as necessary, and may be stored in the culture vessel. The timing of insertion may be at the same time as ear collection, or at an appropriate time after ear collection (for example, within 6 months, 5 months, 4 months, 3 months, or 2 months after ear collection). (within a month) is acceptable, but it is preferable to do so at the same time as ear collection. When inserting at an appropriate time after ear collection, it is preferable to refrigerate the cuttings (for example, 4° C. or lower) until the time of inserting. Thereby, the rooting ability of cuttings can be maintained.

[Support and additive for rooting]
The support only needs to be able to support (hold) the cuttings and the additives used as necessary, has water absorption and air permeability, and can efficiently absorb the additives into the cuttings. Any conventional support can be used. Examples of the support are the same as those of the support that can be used for the growth of the mother tree, and are preferably a combination of natural soil and artificial soil. Akadama soil is preferable as the natural soil. Peat moss is preferred as the artificial soil.

The additive may be any additive used during rooting of cuttings, such as fertilizers (e.g., inorganic components, silver ions, antioxidants, carbon sources, vitamins, amino acids, plant hormones), Rooting promoters other than the above (eg, agents described in documents such as WO 2011/136285, JP-A-2012-232907, JP-A-2013-95664) and the like. The form of each component is not particularly limited, and may be solid (eg, powder, granule) or liquid (eg, liquid fertilizer). In addition, the components constituting the additive may be mixed, absorbed or sprayed on the support, or may be directly spread, coated or sprayed on at least part of the cuttings.

The additives may be mixed with each other or at least partially separately contained in the support, or may be added to a known medium for plant tissue culture or an aqueous solvent (e.g., water) and added to the support. It may be included or applied directly to the cuttings (preferably at the base). Plant tissue culture media include, for example, MS medium, Linsmeyer-Skoog medium, White medium, Gamborg's B-5 medium, and Nitsch-Nich medium. Among them, MS medium and Gumborg's B-5 medium are preferable. These media can be used by appropriately diluting them as necessary. The medium may be either a liquid medium or a solid medium, but a liquid medium is preferable in terms of working efficiency and less damage to roots during transplantation. The liquid medium can be used as it is after preparing by mixing the medium composition. The solid medium can be used by mixing the medium composition in the same manner as the liquid medium and solidifying it with a solidifying agent (eg, agar, gellan gum) at the same time or after preparation. The amount of the solidifying agent to be added can be appropriately set according to conditions such as the type of solidifying agent and the composition of the medium. The amount of agar added to the medium is preferably 0.5 to 1% by weight. The amount of gellan gum added to the medium is preferably 0.2 to 0.3% by weight.

Examples of the inorganic component are the same as those of the fertilizer of the mother tree for harvesting. The inorganic component may be one kind alone or a combination of two or more kinds, and may contain at least one selected from nitrogen, phosphorus, potassium, inorganic salts containing nitrogen, inorganic salts containing phosphorus, and inorganic salts containing potassium. preferable. When one type of inorganic component is included in the above-described known medium, the amount in the medium is preferably 0.1 μM to 100 mM, more preferably 1 μM to 100 mM. In the case of a combination of two or more, the amount in the medium is preferably 0.1 μM to 100 mM, more preferably 1 μM to 100 mM.

Examples of silver ions include silver compounds (silver ion sources) such as silver thiosulfate (STS, AgS ₄ O ₆ ) and silver nitrate, which may be used alone or in combination of two or more, with STS being preferred. STS takes the form of silver thiosulfate ions in the medium and is presumed to be negatively charged, which can contribute to promoting healthy rooting and elongation. When silver ions are contained in the medium described above, the amount of the silver ion source in the medium is preferably 0.5 μM to 6 μM, more preferably 2 μM to 6 μM.

Examples of the antioxidant are the same as those of the fertilizer for the mother tree for harvesting, and may be used alone or in combination of two or more. When an antioxidant is contained in the medium, its amount is preferably 5 mg/l to 200 mg/l, more preferably 20 mg/l to 100 mg/l.

Examples of the carbon source are the same as those of the fertilizer for the mother tree for harvesting, and may be of one type alone or a combination of two or more types. When the carbon source is contained in the medium, the amount is preferably 1 g/l to 100 g/l, more preferably 10 g/l to 100 g/l. When rooting culture is performed by supplying carbon dioxide gas, the rooting medium does not have to contain a carbon source, and preferably does not contain a carbon source. Organic compounds such as sucrose that can be a carbon source also serve as a carbon source for microorganisms, so when using a rooting medium supplemented with these, it is necessary to cultivate in a sterile environment, but carbon dioxide gas is supplied for rooting. By culturing, the addition of a carbon source to the rooting medium can be omitted, and cultivation under a non-sterile environment becomes possible.

Examples of the vitamins are the same as the examples of the vitamins of the fertilizer for the seedling mother tree, and they may be of one kind alone or in combination of two or more kinds. When one vitamin is added to the above medium, the amount is preferably 0.01 mg/l to 200 mg/l, more preferably 0.02 mg/l to 100 mg/l. When two or more types are added in combination, the amount of each is preferably 0.01 mg/l to 150 mg/l, more preferably 0.02 mg/l to 100 mg/l.

Examples of the amino acids are the same as those of the fertilizer for the seedling mother tree, and they may be used alone or in combination of two or more. Amino acids include, for example, glycine, alanine, glutamic acid, cysteine, phenylalanine and/or lysine. When one type of amino acid is added to the above medium, the amount is preferably 0.1 mg/l to 1000 mg/l, and when two or more types are added in combination, the amount of each in the medium is 0.2 mg. /l to 1000 mg/l are preferred.

Plant hormones include, for example, rooting promoters such as auxins and cytokinins. Examples of auxin include naphthaleneacetic acid (NAA), indoleacetic acid (IAA), p-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2,4D), indolebutyric acid (IBA) and derivatives thereof. , one of these, or a combination of two or more. Cytokinins include, for example, benzyladenine (BA), kinetin, zeatin and derivatives thereof, and may be one or a combination of two or more thereof. Plant hormones may be one or a combination of two or more, and preferably include auxin or a combination of auxin and cytokinin.

When a plant hormone is added to one type of medium, the amount is preferably 0.001 mg/l to 10 mg/l, more preferably 0.01 mg/l to 10 mg/l. When two or more types are added in combination, the amount of each is preferably 0.001 mg/l to 10 mg/l, more preferably 0.01 mg/l to 10 mg/l. The method of adding the plant hormone may be according to the instructions of the commercial product. For example, plant hormone powder (eg, auxin) is directly applied to the base of the cutting (preferably the base to which physical stimulation is applied) before inserting. Examples include a coating method and a method of adding to a support.

The timing of addition of the rooting medium is not particularly limited, and includes, for example, at the start of rooting culture and during the culture. The method of addition depends on the aspect of the component, but includes, for example, spraying, wetting, and spraying. The number of additions is also not particularly limited, and may be only once (at the start of culture) or twice or more (at the start and during culture). In addition, the components constituting the rooting medium may be added all at once, may be added separately, or may be appropriately replaced or replenished along the way.

[Culture container for rooting]
By storing the support in the culture container, it is possible to smoothly raise seedlings of cuttings after rooting. The culture vessel preferably has a water passage (mesh, pores). Thereby, it can be used for bottom irrigation. For example, containers (eg, containers described in JP-A-2017-079706, multi-cavity containers (JFA-150, JFA-300), etc.), cell trays, seedling pots, planters, and bats (net-like on the bottom or side) A box-shaped container having an opening may be used, and it may be a type of culture container in which one cutting is planted in one container, or a type of culture container in which two or more cuttings are planted in one container. The material of is not particularly limited, and examples thereof include resin, glass, and wood.

[Inserting cuttings]
The method of attaching the cuttings to the support may be appropriately selected depending on conditions such as the type of support, the environment, and the type of cuttings. For example, there is a method of inserting a part of the cutting including the base (for example, 2 cm to 5 cm from the base) into the support. The base of the cutting means one end of the cutting where the roots are formed (opposite to the end where the leaves are formed). The base of the multi-bud is the region with the cutting surface for dividing the multi-bud. When inserting, a physical stimulus may be applied to the cutting (eg, scratching the base). Thereby, the rooting rate can be improved. The size (eg, size, shape) of the scratch on the base is not particularly limited. For example, a cross-shaped wound can be made on the base (the above-mentioned cut surface) of the multi-bud, which is a cutting. Examples of tools for scratching include scissors and knives. It is preferable to cut off the leaves of the portion of the base of the cutting that will be inserted into the support.

[Irrigation]
As a watering method for rooting, for example, either overhead watering or bottom watering may be used, but bottom watering is preferable. As a method of irrigating the bottom surface, for example, a method of immersing a culture vessel (equipped with a water passage) containing a support to which cuttings are inserted is immersed in water. The amount of irrigation is not particularly limited as long as the cuttings are substantially moistened. In the rooting culture step, the cuttings are watered through the water absorbent member. That is, water is supplied to the water-absorbing member, and water is supplied to the cuttings through the portion where the culture medium and the water-absorbing member are in contact with each other. Water is preferably supplied to the water-absorbing member so that the culture medium is moistened and/or the water-absorbing member uniformly absorbs water. As a result, the moisture environment of the culture medium can be kept moderate, constant and uniform. The irrigation work may be performed by either manual irrigation or automatic irrigation equipment.

[Rooting culture period]
The rooting culture period for rooting the cuttings varies depending on the plant species, but may be continued at least until rooting is observed, and preferably until the roots are well established. It is usually 2 weeks to 10 months, preferably 4 weeks to 8 months, more preferably 2 months to 8 months.

[Other conditions for rooting]
Conditions for rooting other than those mentioned above (e.g., temperature, light, carbon dioxide gas concentration, humidity) can be appropriately determined according to the plant species, part, size, type of additives, etc. of the cuttings, and are generally defined. difficult to do. More preferably, the temperature is, for example, 23-28°C. The light applied to the cuttings may be natural light or light whose light intensity is artificially adjusted. Methods for artificial adjustment include, for example, adjustment of light intensity, adjustment of wavelength components, and light shielding. The light intensity (photosynthetically effective photon flux density) is preferably 10 μmol/m ² /s to 1000 μmol/m ² /s, more preferably 50 μmol/m ² /s to 500 μmol/m ² /s. The light to be irradiated is preferably light containing a wavelength component of 650 nm to 670 nm and a wavelength component of 450 nm to 470 nm, and the ratio between the two is preferably 9:1 to 7:3, more preferably 9:1 to 8:2. is. It is preferable to shield from light. The light shielding rate is preferably 30-70%, more preferably 40-60%.

The carbon dioxide gas concentration during rooting is usually 300 to 2000 ppm, preferably 800 to 1500 ppm. The carbon dioxide concentration is adjusted to the above range by using a culture vessel equipped with a carbon dioxide permeable membrane (for example, placed in a facility such as an artificial climate vessel). be done.

Humidity is preferably 80% or higher, more preferably 85% or higher. This can promote rooting from the plant. There is no particular upper limit. It is preferable to install the culture vessel in a greenhouse. This can facilitate control of conditions such as humidity and temperature.

[Raising seedlings of rooted cuttings]
In the present invention, seedlings are raised after the cuttings are rooted. Raising seedlings may be performed by transplanting from a culture container to a seedling-raising container, a seedling field (use soil: for example, the above-described natural soil), or the like, or may be performed directly in the culture container.

[Fertilization when raising seedlings]
The fertilization method is not particularly limited, and fertilization conditions (fertilization interval, fertilization amount, fertilization method) suitable for the fertilizer to be used may be set. Examples of fertilizer components are the same as those of the fertilizer given to the mother tree.

Conditions for raising seedlings (eg, temperature, humidity, light irradiation, irrigation conditions) can be determined as appropriate. The conditions may be the same as those for rooting culture, or different conditions may be used. When the seedlings have grown to a certain size (for example, 30 cm or more or 35 cm or more), seedlings can be obtained for purposes such as afforestation.

EXAMPLES The present invention will be described with reference to the following examples, but the present invention is not limited thereto.

Example 1
Japanese cedar forest seedlings (1 year old, derived from cuttings) were planted in rows on October 5, 2012 (planting interval 1.5 m×1.5 m). It was grown until February 2015, pruned, and made into a “cylindrical plateau” as a mother tree for harvesting. That is, the trunk was cut off at a height of 2 m, and the branches generating sprouts were pruned to 5 to 15 cm. On May 6 and August 2, 2015, fertilization (fast-acting fertilizer: manufacturer name Akagi Engei, product name chemical fertilizer 8-8-8, each fertilizer amount 50 g / mother tree) was applied to the mother tree. Ta. A 20-cm apical branch was collected from the mother tree in October, three months after the last fertilization, and all the leaves in the lower 5-cm range were cut to prepare cuttings. A cell tray was used as a culture vessel, and Akadama granulated soil (manufactured by Yanashima Shoji Co., Ltd.) and peat moss (manufactured by Toho Co., Ltd.) were mixed in a one-to-one ratio and filled to prepare a cutting bed. Powder of Luton (registered trademark) (manufactured by Ishihara Biosciences Co., Ltd., white powder containing plant hormone NAA, concentration of NAA: 0.4%) was applied to the base (cut portion) of the cutting prepared as described above. After applying 5 to 10 mg, the cuttings were inserted into the cutting bed up to 4 to 5 cm from the base. The plants were placed in a greenhouse and rooted for 8 months until June of the following year. Water was used as the medium. The cuttings after culturing were observed with the naked eye, and if roots were confirmed, it was determined that the cuttings were rooted. After completion of rooting culture, the seedlings were raised outdoors for 4 months. Chemical fertilizer (liquid fertilizer) was sprayed once a week during seedling raising. If the seedlings were alive after the seedling-raising period and the seedling height exceeded 40 cm, it was determined that the seedlings were obtained.

Example 2
The procedure was carried out in the same manner as in Example 1, except that fertilization was performed in May and July, cuttings were collected in November, and rooting culture was carried out for 7 months until June of the following year.

Example 3
The procedure was carried out in the same manner as in Example 1 except that fertilization was performed in May, July and September, cuttings were collected in January of the following year, and rooting culture was carried out for 5 months until June.

Example 4
The same procedure as in Example 1 was carried out, except that fertilization was performed in May, July and September, cuttings were collected in March of the following year, and rooting culture was carried out for three months until June.

Example 5
The same procedure as in Example 1 was carried out, except that fertilization was performed in May, July and September, cuttings were collected in April of the following year, and rooting culture was carried out for two months until June.

Example 6
The procedure was carried out in the same manner as in Example 1, except that fertilization was performed in May and July, cuttings were collected in April of the following year, and rooting culture was carried out for two months until June.

Example 7
The fertilization was carried out in May and July, and the shape of the mother tree for harvesting was tailored to “close-cutting on high ground” (that is, the planting interval was 2 m × 2 m, and the tree height was 1.5 m. The procedure was carried out in the same manner as in Example 1, except that the upper branches were pruned to 20-30 cm, the middle branches were pruned to 30-50 cm, and the lower branches were pruned to 50-60 cm) and the cutting size was 40 cm.

Comparative example 1
The procedure was carried out in the same manner as in Example 1, except that fertilization was performed only in May, cuttings were collected in March of the following year, and rooting culture was carried out for three months until June.

Comparative example 2
It was carried out in the same manner as in Example 1, except that fertilization was performed in May, July and September.

From the results in Table 1, compared with Comparative Examples 1 and 2 in which the ears were collected 10 months or 1 month after the last fertilization, the ears were collected and inserted 2 to 9 months after the last fertilization. In Examples, it can be seen that a good rooting rate can be obtained. This result indicates that the present invention can produce a mother tree for obtaining cuttings with a good rooting rate.

Claims (4)

Applying fertilizer containing a fast-acting fertilizer to the mother tree for harvesting two or three times in one year before the scheduled date of harvesting from the mother tree,
The fertilization suspension period from the last fertilization before the scheduled harvesting date to the transplanting should be 4 months or more and 6 months or less,
The interval between each fertilization is 1.5 to 3 months,
Before the first fertilization within one year before the scheduled date of ear collection , cut the mother tree so that the tree height after cutting is 0.9 m or more and 2.5 m or less, and the size of the branch after pruning is including pruning to a height of 3 cm or more and less than 20 cm,
The mother tree for collecting ears is cedar,
The scheduled harvesting date is a month when the average temperature is 20°C or less.
Production method of the mother tree for harvesting. The production method according to claim 1, wherein the interval between each fertilization is 2-3 months. 3. The production method according to claim 1 or 2, wherein the scheduled ear collection date is from October to April. To obtain cuttings by collecting ears from the mother tree produced by the production method according to any one of claims 1 to 3 on or near the scheduled date for collecting ears, and the size of the cuttings shall be 25 cm or less. , rooting the cuttings, and raising the rooted cuttings.