JP6951896B2 - How to produce plant cuttings - Google Patents

Description

The present invention relates to a method for producing plant cuttings.

Cuttings are a method of rooting in the rooting bed using artificially cut plant tissue to produce one independent plant, which is an excellent method for mass growth of genetically uniform seedlings. Is. In addition, it is easy to grow by cuttings, it is suitable for producing a large amount of seedlings at one time, and it is a commercially advantageous method because it can grow a large number of individuals with excellent traits.

Patent Document 1 describes that in the production of cuttings, irrigation is performed from the bottom surface of the soil in which the cuttings are inserted to supply water, and irrigation is performed by immersion. The reason for adopting irrigation is that when trying to avoid over-humidity with overhead irrigation, water does not spread evenly to the hilling, the variation in water content between the hilling parts tends to increase, and the hilling tends to dry. Is described.

Non-Patent Document 1 describes that air root cutting is performed to prevent looping of seedlings.

JP-A-2015-27262

Koji New Year et al. "Effects of air root cutting treatment on the bottom of the pot on the root system growth of seedlings" Nissoku Kogyo Shimbun 37 (1), 143-166 (2011)

In the method of Patent Document 1, since irrigation is performed by immersion, the water environment varies, which may reduce the rooting rate. Further, by irrigating by immersion, the work of supplying water to each immersion container may become complicated. Further, in the method of Patent Document 1, looping occurs because air root cutting is not performed, and there is a possibility that survival after planting is reduced due to this.

An object of the present invention is to provide a method for producing plant cuttings with improved rooting rate and survival after planting.

The present invention provides the following [1] to [11].
[1] A rooting culture step in which a medium into which cuttings are inserted is installed so that at least a part of the medium is in contact with a water-absorbing member, irrigation is performed through the water-absorbing member, and rooting is performed from the cuttings.
How to produce plant cuttings, including.
[2] In the rooting culture step, the medium is stored in a culture vessel having an opening at least substantially at the bottom, and the culture vessel is placed on the water-absorbing member so that the medium is in contact with the water-absorbing member through the opening. The method of [1], which is a step of performing irrigation.
[3] Further, a medium in which the cuttings after rooting are inserted is installed so that at least a part of the medium is in contact with the air layer, and the cuttings are raised to obtain cuttings, [1] or Method of [2].
[4] The seedling raising step is a step in which the medium is stored in a container having an opening at least substantially at the bottom, the medium is installed so that the opening is in contact with the air layer, and the cuttings are raised to obtain cuttings. , [3] method.
[5] The method according to any one of [1] to [4], wherein the water-absorbent member is in the form of a sheet or a mat.
[6] The method according to any one of [1] to [5], wherein the water-absorbent member is made of a non-woven fabric.
[7] The method according to any one of [1] to [6], wherein the waterproof member is installed at least below the water-absorbent member.
[8] The method according to any one of [1] to [7], wherein the air layer is a void in contact with substantially the bottom surface of the medium.
[9] The method according to any one of [1] to [8], wherein the rooting culture step is carried out for 2 weeks to 10 months.
[10] The method according to any one of [1] to [9], wherein the plant is a plant of the genus Cryptomeria, a plant of the genus Hinoki, a plant of the family Pinaceae or a plant of the genus Eucalyptus.
[11] Medium for inserting cuttings,
Culture container for storing the medium in which the cuttings are inserted,
A kit for raising seedlings of plant cuttings for carrying out any of the methods [1] to [10] for pre-rooting cuttings, including a water-absorbing member and a tray, stand or container.

According to the present invention, the quality of cuttings can be improved. Specifically, by using the method of the present invention, the water environment can be made uniform, and thus the rooting rate can be improved. Furthermore, the method of the present invention can reduce the complexity of work as compared with irrigation by immersion.

The present invention provides a method for producing plant cuttings, which includes a rooting culture step and a seedling raising step.

[Rooting culture process]
The rooting culture step is a step in which a medium in which cuttings are inserted is installed so that at least a part of the medium is in contact with the water-absorbing member, irrigation is performed through the water-absorbing member, and rooting is performed from the cuttings. be.

(Cutting)
The cuttings may be the cuttings of the plant for which cutting seedlings are to be obtained. The type of plant is not particularly limited. Plants can be classified into woody plants and herbaceous plants, but this plant can be applied to any of these, preferably applied to woody plants, and has inferior rooting ability to herbaceous plants. More preferably, it is applied to woody plants. As woody plants, Sugi (Cryptomeria) plants (Cryptomeria japonica, etc.), Hinoki (Chamaecyparis) plants (Hinoki (Chamaecyparis obtusa), etc.), Pine pine (Pine pine) plants (Pinus sunbergii, etc.), Lalix plants (Lalix kaempferi, Lalix gmelini, etc.), Fir (Abies) plants (Abies, etc.) Eucalyptus plants, Prunus plants (Prunus spp., Prunus mume, Prunus tomentosa, etc.), Mangifera plants (Mangifera, etc.) Aquacia plants, Myrica plants, Quercus plants (Quercus actusima), Vitis plants, Malus plants, Rose plants, Tsubaki plants (Rosa) Camellia plants (such as Camellia sinensis), Jacaranda plants (such as Jacaranda mimosifolia), crocodile plants (Persea) plants (Avocado, Persia america), etc. (Pyrus serotina Rehder, Pyrus pyrifolia, etc.), Santalum plants (Byakudan (sandalwood; Santarum album), etc.) are exemplified. Of these, for cedar, cypress, pine (black pine, larch, gmelin, fir, etc.), eucalyptus, cherry, mango, avocado, acacia, bayberry, kunugi, grape, apple, rose, camellia, tea, plum, tomentosa, jakaranta, etc. When applied, the effects of the present invention can be more exerted. Among them, Sugi genus plants, Hinoki genus plants, Matsu family plants (Pine genus plants, Karamatsu genus plants, Fir genus plants, etc.), Eucalyptus plants, Tsubaki genus plants, Mango genus plants, Crocodile genus plants are preferable, and Sugi genus plants, Hinoki The genus plant, the pine genus plant, the karamatsu genus plant, the fir genus plant, and the eucalyptus genus plant are more preferable, and the sugi genus plant, the hinoki genus plant, the pine genus plant, the karamatsu genus plant, and the fir genus plant are further preferable.

The cuttings may be at least a part of the plant, and branches such as green branches (current year branches) and mature branches (branches that grew before the previous year); shoots such as apical buds and axillary buds; leaves, cotyledons; hypocotyls, etc. Is illustrated. The cuttings in the case of woody plants are usually green or mature branches, and the cuttings in the case of herbaceous plants are usually leaves or buds, but are not limited thereto.

Shoots may be used as cuttings from the viewpoint that they are expected to form adventitious roots. Shoot refers to all tissues that have rooting ability. Examples of the tissue include branches, stems, apical buds, axillary buds, adventitious buds, leaves, cotyledons, hypocotyls, adventitious embryos, shoot primordia and the like. The origin of the shoot is not particularly limited, and it may be a tissue obtained from an individual plant growing in a greenhouse or outdoors, a cultured tissue obtained by a tissue culture method, or a part of a natural plant body. It may be an organization of. Shoots can be efficiently obtained from the mother plant of cuttings or polyblasts. Of these, cuttings (cuttings obtained from the mother plant) are preferable.

The polyblast can be derived by cutting tissues such as apical buds and axillary buds from a plant for which a cloned seedling is to be produced by applying the present invention, and tissue culturing the tissues. The polyblast can be formed by aseptically culturing an organ collected from the mother plant according to the method and conditions described in JP-A-8-228621. The method and conditions are as follows. First, tissues such as apical buds and axillary buds are collected from the plant as a material, and the collected tissues have an aqueous solution of sodium hypochlorite having an effective chlorine content of about 0.5% to about 4% or an effective chlorine content of about 5% to. The surface is sterilized by immersing it in an aqueous solution of hydrogen peroxide of about 15% for about 10 minutes to about 20 minutes. Then, this is washed with sterile water, inserted into a solid medium to open the buds, and the elongated foliage is subcultured in a medium having the same composition to form polyblasts. When a tissue of the genus Eucalyptus or the genus Acacia (for example, axillary bud) is used, the solid medium contains 1 to 5% by weight of sucrose, and benzyladenine (hereinafter abbreviated as BA) as a plant hormone is about 0.02 mg / L or more. Murashige sucrose (hereinafter abbreviated as MS) medium containing 1 mg / L or less, about 0.2% by weight or more and about 0.3% by weight of gerangum, or about 0.5% by weight or more and about 1% by weight or less of acacia, or MS. It is preferable to use a modified MS medium in which the ammonium nitrate component and the potassium nitrate component of the medium are halved. Shoots actively grow from the polyblasts formed in this way. The polyblast itself can be maintained and proliferated by appropriately dividing and culturing in a medium having the same composition as the medium used for polyblast formation.

(Culture medium)
The medium is not particularly limited as long as the cuttings are supported and have water absorption and air permeability. For example, the medium may include a support. Thereby, the cuttings can be supported in the rooting medium and the rooting culture can be efficiently carried out. The support is not particularly limited as long as the cuttings can be held in a supported state during the rooting culture step, and a conventional support can be used. As the support, for example, natural soil such as sand, soil (eg, Akadama soil) (preferably Akadama soil); artificial soil such as paddy husk charcoal, coconut fiber, vermiculite, perlite, peat moss, glass beads; , Porous molded products such as rock wool; solidifying agents (eg, agar or gellan gum) and the like. The support may be any one that does not interfere with the contact between the cuttings and the medium, and the support may contain at least a part of the medium.

The medium may contain fertilizer components. Examples of fertilizer components include components that can be sources of plant nutrients such as inorganic components, silver ions, antioxidants, carbon sources, vitamins, amino acids, and plant hormones. The form of the fertilizer component is not particularly limited, and may be either a solid substance (eg, powder, granule) or a liquid (eg, liquid fertilizer).

Examples of the inorganic component include elements such as nitrogen, phosphorus, potassium, sulfur, calcium, magnesium, iron, manganese, zinc, boron, molybdenum, chlorine, iodine and cobalt, and inorganic salts containing these elements. Examples of the inorganic salt include potassium nitrate, ammonium nitrate, ammonium chloride, sodium nitrate, potassium monohydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, ferric sulfate, manganese sulfate, and zinc sulfate. , Copper sulfate, sodium sulfate, calcium chloride, magnesium chloride, boric acid, molybdenum trioxide, sodium molybdate, potassium iodide, cobalt chloride and the like, and hydrates thereof. As the inorganic component, one type may be selected from the above specific examples, or two or more types may be used in combination. The medium used in the present invention preferably contains nitrogen, phosphorus and potassium as essential elements. Therefore, among the specific examples of these inorganic components, nitrogen, phosphorus, potassium, an inorganic salt containing nitrogen, an inorganic salt containing phosphorus, and an inorganic salt containing potassium are preferable, and an inorganic salt containing nitrogen, phosphorus, potassium, and nitrogen is preferable. More preferred. In the case of one kind, the inorganic component is preferably added so as to be about 1 μM to about 100 mM, and more preferably added so as to be about 0.1 μM to about 100 mM. In the case of a combination of two or more kinds, it is preferable to add them so as to be about 0.1 μM to about 100 mM, and more preferably to add them so as to be about 1 μM to about 100 mM.

Examples of the silver ion include silver compounds (silver ion source) such as silver _{thiosulfate (STS, AgS 4} O _{6) and silver nitrate, and STS is preferable.} STS takes the form of silver thiosulfate ions in the medium and is presumed to be negatively charged, which can contribute to the promotion of healthy root rooting and elongation. The concentration of silver ions added to the medium depends on the type of silver ion source and other culture conditions, but the concentration of the silver ion source is preferably about 0.5 μM or more and about 6 μM or less, and about 2 μM or more and about 6 μM or less. More preferred.

Examples of the antioxidant include ascorbic acid and sulfites, and ascorbic acid is preferable. Since ascorbic acid has low persistence in the medium, environmental pollution can be suppressed. The concentration of the antioxidant added to the medium is preferably about 5 mg / l or more and about 200 mg / l or less, and more preferably about 20 mg / l or more and about 100 mg / l or less.

As the carbon source, compounds such as carbohydrates such as sucrose and derivatives thereof; organic acids such as fatty acids; and primary alcohols such as ethanol can be used. As the carbon source, one type may be selected from the above specific examples, or two or more types may be used in combination. The carbon source is preferably added to the medium in an amount of about 1 g / l to about 100 g / l, and more preferably to an amount of about 10 g / l to about 100 g / l. However, when the cultivation is carried out while supplying carbon dioxide gas, the medium does not need to contain a carbon source and is preferably not contained. Organic compounds that can be a carbon source such as sucrose also serve as a carbon source for microorganisms. Therefore, when using a medium containing these, it is necessary to cultivate in a sterile environment, but a medium that does not contain a carbon source is used. This enables cultivation in a non-sterile environment.

As vitamins, for example, biotin, thiamine (vitamin B1), pyridoxine (vitamin B4), pyridoxal, pyridoxamine, calcium pantothenate, inositol, nicotinic acid, nicotinamide and / or riboflavin (vitamin B2) and the like should be used. Can be done. As the vitamins, one type may be selected from the above specific examples, or two or more types may be used in combination. In the case of one type of vitamins, it is preferable to add them to the medium so as to be about 0.01 mg / l to about 200 mg / l, and to add them so as to be about 0.02 mg / l to about 100 mg / l. Is more preferable. In the case of a combination of two or more, it is preferable to add them to the medium so as to be about 0.01 mg / l to about 150 mg / l, and to add them so as to be about 0.02 mg / l to about 100 mg / l. Is more preferable.

As the amino acids, for example, glycine, alanine, glutamic acid, cysteine, phenylalanine and / or lysine can be used. As the amino acids, one type may be selected from the above specific examples, or two or more types may be used in combination. Amino acids are preferably added to the medium in an amount of about 0.1 mg / l to about 1000 mg / l, and in the case of a combination of two or more, each is about 0.2 mg / l to about 1000 mg in the medium. It is preferable to add it so as to be / l.
Examples of plant hormones include rooting promoters such as auxin and cytokinin. Examples of auxins include naphthalene acetic acid (NAA), indoleacetic acid (IAA), p-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2,4D), indolebutyric acid (IBA) and derivatives thereof. One or more or a combination of two or more selected from the above can be used. Examples of cytokinins include benzyladenine (BA), kinetin, zeatin and derivatives thereof, and one or more or a combination of two or more selected from these can be used. The plant hormone is preferably auxin or a combination of auxin and cytokinin.

The concentration of the plant hormone in the medium is preferably 0.001 mg / l to 10 mg / l, more preferably 0.01 mg / l to 10 mg / l when one type of plant hormone is used. When there are two or more plant hormones, the amount is preferably 0.001 mg / l to 10 mg / l, and more preferably 0.01 mg / l to 10 mg / l, respectively. The method of adding the plant hormone is not particularly limited, and it may be added according to the instruction manual of a commercially available product. Examples thereof include a method of applying the plant hormone as it is to the base of cuttings and a method of adding it to a medium.

The medium may be continuously used without being replaced through the method of the present invention, may be replaced in the middle, and is preferably continued to be used without being replaced through the method of the present invention from the viewpoint of convenience of work. The medium may be supplemented with fertilizer components during the process of the present invention.

(Water absorbing member)
The water-absorbent member used in the rooting culture step may be any water-absorbent member, and its shape is not particularly limited, and examples thereof include a mat-like shape and a sheet-like shape. Examples of the water-absorbent member include processed products such as sponges, non-woven fabrics, woven fabrics, and papers (for example, polyester non-woven fabrics) made of a water-absorbent material or a material containing a water-absorbent component. The water-absorbent member may contain any component other than water-absorbent, such as an antibacterial agent, if necessary. Examples of the bottom water supply mat include those described in Japanese Patent Application Laid-Open No. 2013-100269.

(Waterproof member)
It is preferable that a waterproof member is installed at least below the water-absorbent member. As a result, leakage of water from the water-absorbent member can be suppressed, and the cuttings can be efficiently irrigated. The waterproof member may be any member having waterproofness, and its shape is not particularly limited, and examples thereof include a mat shape and a sheet shape. Examples of the waterproof member include a vinyl sheet and a film.

(Position of medium and water-absorbent member)
The medium into which the cuttings are inserted is installed so that at least a part of the medium is in contact with the water-absorbing member. As a result, irrigation in the rooting culture step can be performed via the water-absorbing member. By performing bottom irrigation through the water-absorbing member in this way, the water environment of the cuttings can be maintained appropriately and constant as compared with the conventional bottom irrigation performed by immersing the medium in water and overhead irrigation. , The over-humidity state can be suppressed, the occurrence of diseases can be suppressed, and a good rooting rate can be obtained. In addition, when rooting of a plurality of cuttings at the same time, not only a good rooting rate can be obtained, but also the growth rate such as the rooting time can be made uniform, so that the multiple cuttings can be collectively used in the seedling raising process. It will be possible to migrate.
The portion of the medium in contact with the water-absorbent member may be one or two or more, and preferably includes at least a part of the substantially bottom portion of the medium, and preferably at least a part of the substantially bottom portion of the medium.

(Culture container)
In the rooting culture step, the medium into which the cuttings are inserted is preferably stored in a culture vessel. As a result, it becomes easy to adjust the portion where the medium and the water-absorbing member are in contact with each other, and the transition to the seedling raising step after the rooting culture step can be smoothly performed without complicated work. The culture vessel preferably has an opening at least substantially at the bottom. As a result, bottom irrigation of the medium via the water-absorbing member can be efficiently performed. The position of the opening may be at least a part of the bottom portion (bottom surface and side surface close to the bottom surface). The shape and shape of the opening are not particularly limited, but may be, for example, two or more holes scattered, or a net having one or two or more on the bottom surface. The culture vessel is a container that can store the medium in which one strain of cuttings is inserted, or a unit for inserting the medium in which one strain of cuttings is inserted is connected or divided into the units, and a plurality of cuttings are inserted as a whole. A culture container into which the ears can be inserted is preferable. These containers may be held in the tray as long as the tray does not interfere with the contact point between the medium and the bottom water supply mat.

The culture container is not particularly limited as long as it holds a medium (soil or the like) and has an opening at least substantially at the bottom. For example, a container (eg, a container described in JP-A-2017-079706). , Multi-cavity container (JFA-150, JFA-300), cell tray, nursery pot, planter, and bat (box-shaped container with net-like opening on the bottom or side. Multiple cuttings are inserted together. It may be.). The material of the culture container is also not particularly limited, and examples thereof include resin, glass, and wood.

(Insert)
The method of inserting the cuttings into the medium may be appropriately selected depending on the type of the medium, the culture conditions, and the like. It is also preferable to apply a physical stimulus such as scratching the base of the cutting when it is inserted into the medium in order to improve the rooting rate. The base of the cutting means the region at one end of the cutting where the root is formed (opposite to the end where the leaf is formed). When a polyblast is used as the cutting, the base is a region having a cut surface when the polyblast is divided. The size (size, shape, etc.) of the wound on the base of the cutting is not particularly limited. For example, when a polyblast is used as the cutting, it is preferable to scratch the base of the cutting (the above-mentioned cut surface) so as to form a cross when viewed from the front. When scratching, tools such as scissors and knives can be used.

(Irrigation)
In the rooting culture step, the cuttings are irrigated via a water-absorbing member. That is, water is supplied to the water-absorbent member, and water is supplied to the cuttings through the portion where the medium and the water-absorbent member are in contact with each other. It is preferable that water is supplied to the water-absorbent member so that the medium is moistened and / or the water-absorbent member is in a state of uniformly absorbing water. As a result, the water environment of the medium can be maintained appropriately, constantly and uniformly. The irrigation operation may be performed by either manual irrigation or an automatic irrigation system.

The culture period of the rooting culture step varies depending on the plant species, but is usually 2 weeks to 10 months, preferably 4 weeks to 6 months, and particularly preferably 2 months to 6 months. The rooting culture step may be continued until rooting is observed from the cuttings.

[Seedling raising process]
The seedling raising process is a process of raising seedlings after rooting to obtain cuttings.

(Culture medium)
In the seedling raising step, the medium into which the cuttings after rooting are inserted is installed either so that at least a part of the medium is in contact with the air layer or the medium is not in contact with the air layer. Also, preferably, at least a part of the medium is placed in contact with the air layer. When at least a part of the medium is in contact with the air layer, looping due to air root cutting can be suppressed after rooting of the cuttings, and cuttings with improved survival after planting can be provided. Thereby, the efficiency of cutting seedling production can be improved. The medium may be different from the medium used in the rooting culture step or the same medium, but the same medium is preferable, and the medium used in the rooting culture step is more preferably used as it is in the seedling raising step. .. The example of the medium is the same as the example of the medium described in the rooting culture step.

(container)
In the seedling raising step, the medium is preferably stored in a container. This facilitates the adjustment of the portion where the medium and the air layer are in contact with each other. The container may be a container different from the culture container used in the rooting culture step or the same container, but the same container is preferable, and the culture container used in the rooting culture step may be used as it is in the seedling raising step. More preferred. As a result, the transition to the seedling raising process after rooting culture can be smoothly performed by omitting complicated work. The example of the container is the same as the example of the culture container described in the rooting culture step.

(Position of medium and air layer)
The air layer may be provided so as to be in contact with a part of the medium. The portion of the medium in contact with the air layer may be one or two or more, and preferably contains at least a part of the substantially bottom part of the medium, and preferably at least a part of the substantially bottom part of the medium. The portion of the medium that is in contact with the air layer preferably contains at least the same portion as a part of the medium that was in contact with the water-absorbing member in the rooting culture step, and more preferably the same portion.

(Air layer)
The air layer is provided in contact with a portion of the medium, preferably below substantially the bottom of the medium. The size of the air layer is not particularly limited, but is usually 1 cm or more in the vertical direction from the substantially bottom of the medium, preferably 2 cm or more, and more preferably 3 cm or more. The upper limit is not particularly limited, but can be set as appropriate according to the equipment for raising seedlings. The method of providing the air layer is not particularly limited, but for example, the medium or the container containing the medium is installed on a tray, a stand, a pedestal, or the like, and the container and the ground, or the water-absorbent member used in the rooting culture step. A method of providing a gap between the two is mentioned.

The culture period of the seedling raising process can be appropriately set according to the plant species, the climatic conditions of the transplanting / planting site, and the like.

[Other general conditions]
In the rooting culture step and the seedling raising step, the conditions other than those described above are not particularly limited as long as the cuttings can be rooted and raised. The conditions may be the same or different in the rooting culture step and the seedling raising step. It is difficult to unconditionally define the conditions depending on the type, site, condition, medium type, etc. of the cuttings, but an example will be described below.

(Location of rooting and raising seedlings)
The rooting and raising of seedlings may be in a closed space (eg, in a greenhouse, in a carbon dioxide culture room, in a greenhouse, indoors) or in an open space (eg, outdoors), but a closed space is preferred. This makes it easy to adjust conditions such as temperature and humidity.

(Temperature of rooting and raising seedlings)
The temperature in the environment for rooting and raising seedlings is not particularly limited as long as the conditions for rooting and raising seedlings of cuttings are possible, but is preferably 20 to 40 ° C., for example.

(PH of medium)
The pH of the medium is preferably 4 to 8, more preferably about pH 4 (for example, pH 4 to 6). This makes it possible to suppress the growth of germs and the like. The pH of the rooting medium and the seedling raising medium may be different or may be the same.

(Amount of light)
The light irradiation conditions are not particularly limited, and sunlight may be used or artificial light may be used. The light intensity is not particularly limited, photosynthetic expressed as photon flux density is preferably not more than about 10μmol / m ² / s or more to about 1000 micro mol / m ² / s, about 50μmol / m ² / s or more to about 500 [mu] mol / More preferably, it is m ^{2 / s or less.}

The light wavelength is not particularly limited, but it is preferable to carry out the irradiation under irradiation of light containing a wavelength component of about 650 nm or more and about 670 nm or less and a wavelength component of about 450 nm or more and about 470 nm or less in a ratio of 9: 1 to 7: 3. It is more preferable to carry out the irradiation under irradiation of light containing these wavelength components at a ratio of 9: 1 to 8: 2. By irradiating light containing such a wavelength component, rooting from a plant can be further promoted.

In the rooting culture step, it is preferable to perform shading. The shading rate is preferably 30% or more and 70% or less, and more preferably 40% or more and 60% or less.

(Carbon dioxide concentration)
The carbon dioxide gas in the rooting and raising seedling environment is usually preferably supplied in an amount of 300 ppm or more and 2000 ppm or less, preferably 800 ppm or more and 1500 ppm or less. The amount of carbon dioxide gas supplied can be controlled by using equipment such as an artificial meteorological instrument or a culture vessel having a carbon dioxide permeable membrane at the opening.

Humidity can be adjusted according to conditions such as the type of plant, but is usually 50% or more, preferably 60% or more. As a result, rooting from plants can be promoted and seedlings can be raised efficiently. There is no particular limit on the upper limit.

[Kit for raising seedlings of plant cuttings]
The method of the present invention comprises a medium for inserting cuttings, a culture vessel for storing the medium into which cuttings have been inserted, a water-absorbing member, and a kit for raising seedlings of plant cuttings, which includes a tray, a stand or a stand. , Can be preferably carried out. Examples of the medium, the culture vessel, the water-absorbent member, the tray, the stand, and the gantry are the same as those described in the above-mentioned method of the present invention. The kit of the present invention may or may not further include pre-rooted cuttings. The kit of the present invention may further include a waterproof member. Further, as the medium and the culture vessel are preferably used in both steps as described in the method of the present invention, it is not necessary to prepare them for each step, but they may be prepared for each step. good.

Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited thereto.

[Example 1]
(Preparation of cutting bed)
As a culture container, a single cavity type culture container (NP container, JP-A-2017-079706, with an opening on the bottom surface) held in a cell tray was used. As a medium, Akatama small-grain soil (manufactured by Yasushima Shoji Co., Ltd.) and peat moss (manufactured by Toho Co., Ltd.) are mixed 1: 1. "(Manufactured by Otsuka Agrio Techno Co., Ltd.)) was mixed at a ratio of 5 g / L to prepare a medium. The prepared medium was filled in a culture container to prepare a bed. The prepared cutting bed was irrigated and the medium was sufficiently moistened to prepare a cutting bed to be used for cutting.

(Preparation and cutting of cuttings)
A 20 cm apical bud branch to be a cutting was collected from a perennial branch of Sugi (Tanoaka; Cryptomeria japonica), and all the leaves in the lower 5 cm range were cut to prepare cuttings. A powder of Luton (registered trademark) (manufactured by Ishihara Biosciences Co., Ltd., white powder containing the plant hormone α-naphthylacetamide (NAA), NAA concentration 0.4%) at the base (cut portion) of the prepared cuttings. After applying 5 to 10 mg of the cuttings, the cuttings were inserted up to 4 to 5 cm from the base and inserted into the floor.

(Rooting culture process)
Rooting culture was performed in a greenhouse. A vinyl sheet was laid on the ground of the greenhouse for waterproofing. A bottom water supply mat (trade name: Unitika Love Mat U, polyester long fiber non-woven fabric, thickness 2.2 mm) was installed on the vinyl sheet. On the bottom water supply mat, a cell tray holding the culture container in which Sugi was inserted was installed so that the bottom surface of the container directly touched the water supply mat. The irrigation was not carried out directly on the plant body and the container, but was carried out by irrigating the bottom water supply mat until the bottom water supply mat was sufficiently wet. The medium was moistened by irrigation using a bottom water supply mat. The irrigation work was performed by either manual irrigation or an automatic irrigation system. Irrigation was carried out for about 5 months when rooting was confirmed.

(Seedling raising process)
After rooting culture, seedlings were continuously raised in a greenhouse for 9 months. In the seedling raising process, the bottom water supply mat and the vinyl sheet were not installed, the container was moved from the cell tray to the pedestal, and an air layer of about 3 cm was provided between the ground and the container. By providing the air layer, when the roots grow after rooting in the container, the roots stop growing when they reach the air layer from the bottom of the container, and the phenomenon of air root cutting occurs. In the main seedling raising step, irrigation was performed by overhead irrigation of the plant.

[Example 2]
In the seedling raising step, the same procedure as in Example 1 was carried out including the rooting step, except that the container was not moved to the gantry and was subsequently irrigated using the bottom water supply mat so that the soil inside the container was moistened. ..

[Comparative Example 1]
As an irrigation method for the rooting step, the container is constantly immersed in a container filled with water, and each time the water in the container becomes low, the container is manually irrigated, except that the seedling raising step is included in the same manner as in Example 1. carried out.

[Comparative Example 2]
As an irrigation method in the rooting step, the container was constantly immersed in a container filled with water, and each time the water in the container became low, the container was manually irrigated. Further, in the seedling raising process, the container was not relocated to the gantry, and the soil in the container was continuously irrigated by using the water supply mat for the bottom surface. Other than those, it was carried out in the same manner as in Example 1.

[Comparative Example 3]
As a method of irrigating the rooting step, the same procedure as in Example 1 was carried out including the seedling raising step except that the plant was directly irrigated overhead.

[Comparative Example 4]
As a method of irrigation in the rooting process, the plants were directly irrigated overhead. Further, in the seedling raising process, the container was not relocated to the gantry, and the soil in the container was continuously irrigated by using the water supply mat for the bottom surface. Other than those, it was carried out in the same manner as in Example 1.

[Evaluation items and results]
In Examples and Comparative Examples, the labor of irrigation, rooting rate, and survival after planting were evaluated. The results are shown in Table 1.

From the results shown in Table 1, it was shown that the rooting culture and seedling raising under the conditions of Example 1 or 2 had an effect on the labor of irrigation and the rooting rate as compared with Comparative Examples 1 to 4. .. Furthermore, it was shown that rooting culture and seedling raising under the conditions of Example 1 have an effect on survival after planting in addition to labor and rooting rate of irrigation as compared with Comparative Examples 1 to 4. rice field.

Claims (6)

The medium into which the cuttings of the woody plant are inserted is stored in a culture vessel having an opening at least substantially at the bottom, and the culture vessel is water-absorbent so that a part of the medium is in contact with the water-absorbing member through the opening. A rooting culture step of placing on a member, irrigating through a water-absorbing member, and rooting from cuttings, and
The medium into which the cuttings were inserted after rooting was stored in a container having an opening at least at the bottom, and the medium was placed so that the bottom of the medium was in contact with the air layer through the opening, and overhead irrigation was performed. A method for producing cuttings of a plant, which includes a seedling raising step of raising seedlings of cuttings to obtain cuttings. The method according to claim 1 , wherein the water-absorbent member is in the form of a sheet or a mat. The method according to claim 1 or 2 , wherein the water-absorbent member is made of a non-woven fabric. The method according to any one of claims 1 to 3 , wherein a waterproof member is installed at least below the water-absorbent member. The method according to any one of claims 1 to 4 , wherein the rooting culture step is carried out for 2 weeks to 10 months. The method according to any one of claims 1 to 5 , wherein the woody plant is a plant of the genus Sugi, a plant of the genus False cypress, a plant of the family Pinaceae, a plant of the genus Eucalyptus or a plant of the genus Camellia.