JP6726482B2 - Rooting method and rooting equipment - Google Patents

Description

The present invention relates to a rooting method and rooting equipment.

Cutting is a method of rooting in the rooting bed using artificially cut plant tissue (cutting) to produce one independent plant body, and mass-proliferates genetically uniform seedlings. An excellent way to

Propagation by cuttings is simple, suitable for producing a large amount of seedlings at one time, and it is considered to be a commercially advantageous method because a large amount of individuals having excellent traits can be propagated. However, many plant species and varieties are difficult to root by cuttings. As a method for solving such a problem, for example, the method described in Patent Document 1 has been proposed.

In Patent Document 1, a rooting bed moistened with a liquid medium containing nitrogen, phosphorus, and potassium as essential elements and containing no carbon source is prepared in a culture container capable of supplying carbon dioxide, and inserted into this. A method is disclosed in which the ear is inserted and cultured, and the rooting from the ear is performed while controlling the carbon dioxide concentration in the culture container.

Japanese Patent No. 3861542

According to the method described in Patent Document 1, the rooting rate of cuttings can be increased, but an advanced culture equipment or the like for supplying carbon dioxide gas to a culture container is required, and the equipment is widely used for deployment. There was a demand for simplification.

It is an object of the present invention to provide a rooting method that can be applied to plants that have difficulty in rooting and that can simplify equipment.

As a result of diligent studies to solve the above problems, the present inventors absorbed at least a part of light having a wavelength range of 400 nm or less among the light irradiated to the cuttings in the step of rooting from the cuttings. Radiation of light in the wavelength range of 550 nm to 700 nm, or absorption of at least part of the light in the wavelength range of 700 nm to 1000 nm among the light radiated to the cuttings, not only plants with easy rooting, It has been found that the rooting rate can be improved even in plants that have been conventionally difficult to root, and that equipment can be simplified. The present invention is based on this novel finding.

The present invention provides the following [1] to [7].
[1] In the step of rooting from the cuttings, among the light radiated to the cuttings, at least a part of light having a wavelength range of 400 nm or less is absorbed to emit light having a wavelength range of 550 nm to 700 nm, or A rooting method for causing rooting from the cuttings by absorbing at least a part of light having a wavelength range of 700 nm to 1000 nm among the lights irradiated to the cuttings.
[2] In the step of rooting from cuttings, among the light radiated to the cuttings, a light exchange material that absorbs at least a part of light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 nm to 700 nm, Or, of the light radiated to the cuttings, at least a part of the cuttings is covered with a covering material that includes a light absorbing material that absorbs at least a part of the light in the wavelength range of 700 nm to 1000 nm, and emitted from the cuttings. The rooting method according to the above [1], which comprises rooting.
[3] The rooting method according to the above [2], wherein the cuttings are covered with a covering material and a light shielding material arranged so as to overlap the covering material.
[4] The rooting method according to [2] or [3] above, which covers the upper and lateral sides of the cuttings with a covering material.
[5] The rooting method according to any one of [1] to [4] above, wherein the cuttings are cuttings of a plant selected from cherry, apple, eucalyptus, cedar and pine.
[6] A rooting equipment for rooting from cuttings, comprising a covering material for covering at least a part of the cuttings, wherein the covering material has a wavelength range of 400 nm or less in light irradiated to the cuttings. A light conversion material that absorbs at least a part of light and emits light in a wavelength range of 550 nm to 700 nm, or a light absorption that absorbs at least a part of light in a wavelength range of 700 nm to 1000 nm among lights irradiated to cuttings. Rooting equipment with wood.
[7] The rooting equipment according to the above [6], which comprises a light-shielding material that covers the cuttings together with the covering material.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a rooting method that can be applied to a plant in which rooting is difficult and that can simplify equipment.

FIG. 1 is a cross-sectional view of the rooting equipment used in the examples. FIG. 2 is a perspective view of the rooting equipment used in the examples.

The plant to which the present invention is applicable is not particularly limited, for example, eucalyptus, cedar, pine, acacia, bayberry, kunugi, grape, apple, cherry, rose, camellia, wood plants such as plum, chrysanthemum and carnation etc. Herbaceous plants and the like. INDUSTRIAL APPLICABILITY The present invention can be applied to plants that have been conventionally difficult to root and plants that do not. From the viewpoint that roots can be rooted even in plants that have been difficult to root by conventional methods, cherry, apple, eucalyptus, cedar, and pine are preferable as plants to which the present invention is applied.

The cuttings may be at least a part of the plant, such as green branches (current branch), mature branches (branches grown before the previous year), shoots such as apical buds, axillary buds, leaves, cotyledons, hypocotyls. Are exemplified. The cuttings in the case of woody plants are usually green branches or mature branches, and the cuttings in the case of herbaceous plants are usually leaves or shoots, but are not limited thereto.

From the viewpoint that it is expected to form adventitious roots, shoots may be used as cuttings. The shoot refers to all tissues having rooting ability. Examples of the tissue include branches, stems, apical buds, axillary buds, adventitious buds, leaves, cotyledons, hypocotyls, adventitious embryos, shoot primordia, and the like. The origin of the shoot is not particularly limited, and it may be a tissue obtained from a plant individual growing in a greenhouse or outdoors, or a cultured tissue obtained by a tissue culture method, or a part of a natural plant body. May be an organization. The shoots can be efficiently obtained from the mother plant of the cuttings or the multiple shoots. Among them, cuttings (cuttings obtained from the mother plant), a polyblast obtained by aseptically culturing an organ collected from the mother plant, or a foliage obtained by aseptically growing the organ. It is preferable to have.

The multibud body can be induced by cutting off tissues such as apical buds and axillary buds from a plant for which a clone seedling is to be produced by applying the present invention and culturing the tissue. In order to form the multiblasts by aseptically culturing an organ collected from the mother plant, the method and conditions described in JP-A-8-228621 can be used. The method and conditions are as follows. First, tissues such as apical buds and axillary buds are collected from a plant as a material, and the collected tissues have an effective chlorine amount of about 0.5% to about 4% sodium hypochlorite aqueous solution or an effective chlorine amount of about 5%. The surface is sterilized by immersing it in an aqueous solution of about 15% hydrogen peroxide for about 10 minutes to about 20 minutes. Then, this is washed with sterilized water, the seeds are inserted into a solid medium to open the buds, and the foliage that has been elongated is subcultured in a medium having the same composition to form a multibud body. When a eucalyptus or acacia tissue (for example, axillary bud) is used, the solid medium is 1 to 5% by weight of sucrose, and benzyladenine (hereinafter abbreviated as BA) as a plant hormone is about 0.02 mg/L or more. Murashige Skoog (hereinafter abbreviated as MS) medium containing 1 mg/L or less, gellan gum of about 0.2% by weight or more and about 0.3% by weight or agar of about 0.5% by weight or more and about 1% by weight or less, or MS. It is preferable to use a modified MS medium in which the ammonium nitrate component and the potassium nitrate component of the medium are halved. Shoots are actively elongated from the thus formed multiblasts. The multiblasts themselves can be maintained and proliferated by appropriately dividing and culturing in a medium having the same composition as the medium used for forming the multiblasts.

In the step of rooting from cuttings, the carbon dioxide concentration in the cultivation environment is usually 300 ppm to 1500 ppm, preferably 500 ppm to 1500 ppm, more preferably 750 ppm to 1250 ppm, and further preferably 900 ppm to 1000 ppm. More preferable. The supply amount of carbon dioxide gas can be controlled by using equipment such as an artificial weather device, a culture vessel having a carbon dioxide permeable membrane in its opening, and the like.

The humidity of the cultivation environment in the step of rooting from cuttings is preferably 60% Rh or more. The humidity can be appropriately adjusted depending on the target plant, and is usually preferably 80% Rh or higher, more preferably 90% Rh or higher. When the humidity is within the above range, rooting from plants can be promoted. The upper limit of humidity is not particularly limited and may be 100% Rh or less. In the case of a plant in which mold easily grows, it is more preferably 80% Rh or less, further preferably 70% Rh or more.

In the step of rooting from the cuttings, a rooting medium is usually used. In the present invention, the rooting medium means a medium used for rooting from cuttings.

The rooting medium may be either a liquid medium or a solid medium. Inorganic components, carbon sources, vitamins, amino acids and plant hormones, fertilizers, and other components compatible with plants can be added to the rooting medium.

Examples of plant hormones include auxin and cytokinin. Examples of auxins include naphthaleneacetic acid (NAA), indoleacetic acid (IAA), p-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2,4D), indolebutyric acid (IBA) and derivatives thereof. .. The auxin may be one kind or a combination of two or more kinds. Examples of cytokinins include benzyladenine (BA), kinetin, zeatin, and their derivatives. The cytokinin may be one kind or a combination of two or more kinds. The plant hormone may be either one of auxin and cytokinin, or a combination of auxin and cytokinin. As a plant hormone, IBA is preferable from the viewpoint of promoting rooting.

The concentration of the plant hormone in the rooting medium is preferably 0.001 mg/l to 10 mg/l when one plant hormone is used, and more preferably 0.01 mg/l to 10 mg/l. preferable. When there are two or more plant hormones, the amount is preferably 0.001 mg/l to 10 mg/l, and more preferably 0.01 mg/l to 10 mg/l.

As the rooting medium, a medium known as a plant tissue culture medium, to which silver ions and/or an antioxidant, a carbon source, or plant hormones are appropriately added, may be used. Examples of known medium for plant tissue culture include MS medium, Rinsmeier-Skoog medium, White medium, Gamborg's B-5 medium, Niche niche medium and the like.

The method of inserting the cuttings into the rooting medium may be appropriately selected depending on the type of medium, culture conditions and the like. When the rooting medium is a solid medium, the roots of the ears may be directly inserted into the rooting medium and cultured. On the other hand, in the case where the rooting medium is a liquid medium, for example, the soil for cuttings described below may be moistened with the rooting medium, and then the base of the cuttings may be inserted and cultured. In addition, it is also preferable to improve the rooting rate by adding a physical stimulus such as scratching the base of the cuttings when the rooting medium is inserted. The base of the cuttings means an area where the roots are formed (opposite to the end where the leaves are formed) at one end of the cuttings. In the case of using a multibud as the cuttings, the base is a region having a cut surface for dividing the multibud. The size (size, shape, etc.) of the scratch on the base of the cuttings is not particularly limited. For example, when a multibud body is used as the cuttings, it is preferable to scratch the base of the cuttings (the above-mentioned cut surface) so as to form a cross when viewed from the front. When making scratches, tools such as scissors and knives can be used.

The soil for the cutting bed is preferably one that can be held with the cuttings pointing to it during the cultivation. When a liquid rooting medium is used for cultivation, it is usually used after infiltrating the medium. Therefore, the medium is preferably one that can be infiltrated with a liquid, and above all, one that can be substantially uniformly moistened with a liquid medium. As the soil for the bed, any conventionally used soil may be used without any particular limitation. Natural soil such as sand and red tama soil for artificial beds; artificial soil such as rice husk charcoal, coconut fiber, vermiculite, perlite, peat moss and glass beads; foamed phenolic resin, porous molded products such as rock wool, etc. Can be illustrated. Of these, Akadama soil is preferred. A bed can be prepared by placing the medium in a culture vessel. When the rooting medium is a solid medium, the rooting bed can be prepared by directly putting the solid medium in a culture container.

In the present invention, it is preferable to use a culture container for containing the rooting medium. As the culture vessel, a conventionally used culture vessel can be used and is not particularly limited. For example, a cell tray, a nursery pot, etc. are illustrated. The culture container may be a closed type or an open type.

When a branch is used as the cuttings, a closed culture container may be used as the culture container. This facilitates placing the cuttings under high humidity, so that the transpiration effect of the leaves attached to the branches can be suppressed, and the conventional partial leaf excision treatment can be omitted, which is preferable.

The culture container may be a container capable of supplying carbon dioxide gas into the container. An example of such a culture container is a container having an opening covered with a carbon dioxide permeable membrane. By using a container having an opening covered with a carbon dioxide permeable membrane, the humidity of the culture environment can be easily adjusted. The shape of the opening is not particularly limited. The material of the carbon dioxide permeable membrane is not particularly limited, and examples thereof include polytetrafluoroethylene. The pore size of the membrane is not particularly limited, and examples thereof include a membrane having a pore size of about 0.1 μm or more and about 1 μm or less.

In the present invention, the temperature of the rooting medium is usually 20°C to 30°C, preferably 22°C to 26°C.

The present invention, in the step of rooting from the cuttings, among the light radiated to the cuttings, absorbs at least a part of the light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 nm to 700 nm, or Of the light with which the cuttings are irradiated, at least a part of light having a wavelength range of 700 nm to 1000 nm is absorbed.

In a preferred embodiment of the present invention, in the step of rooting from the cuttings, among the light radiated to the cuttings, at least a part of the light having a wavelength range of 400 nm or less is absorbed to give light having a wavelength range of 550 nm to 700 nm. At least a part of the cuttings is covered with a light exchanging material that emits light or a covering material that includes a light absorbing material that absorbs at least a part of the light having a wavelength range of 700 nm to 1000 nm among the lights irradiated to the cuttings. And root it from the cuttings. In this aspect, of the light radiated to the cuttings, a light conversion material that absorbs at least a part of light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 nm to 700 nm, or is radiated to cuttings. It is preferable to use a rooting facility provided with a coating material having a light absorbing material that absorbs at least a part of light having a wavelength range of 700 nm to 1000 nm among light.

The light exchange material emits light by irradiation with natural light and/or artificial light, absorbs at least a part of light having a wavelength range of 400 nm or less, and emits light having a wavelength range of 550 nm to 700 nm. is there. By absorbing light in the wavelength range of 400 nm or less and emitting light in the wavelength range of 550 nm to 700 nm, the effect of ultraviolet rays is reduced, and the light that promotes rooting is radiated to the cuttings and the rooting is promoted. Conceivable. The light exchange material preferably absorbs 70% or more of light having a wavelength range of 400 nm or less, and more preferably 80% or more. Further, the light conversion material preferably emits light in the wavelength range of 610 nm to 700 nm from the viewpoint of promoting rooting.

From the viewpoint of preventing the temperature of the cultivation environment for rooting the cuttings and the temperature of the medium from rising, it is preferable that the light conversion material shields light in the wavelength range of 400 nm to 600 nm, and the light in the wavelength range is used. Those that shield 30% or more of light are more preferable, and those that shield 40% or more of light in the wavelength range are still more preferable.

From the viewpoint of preventing the harmful effect of ultraviolet rays in the wavelength range on the cuttings, the light conversion material has an attenuation rate of 5.5 to 12.0% in the wavelength range of 280 nm to 320 nm (UV-B). In addition, it is more preferable that the attenuation factor in the wavelength range of 250 nm to 280 nm (UV-C) is 17.5 to 28.0%.

As the light conversion material, for example, a dye that absorbs light in the wavelength range of 400 nm or less and emits light in the wavelength range of 550 nm to 700 nm, polyester, nylon, polycarbonate, poly(meth)acrylate, polyolefin, polychlorinated material. A film-like or sheet-like resin containing a thermoplastic resin such as vinyl and a translucent resin can be used. In this case, the content ratio of the fluorescent dye to the thermoplastic resin may be 0.001 to 0.03% by mass. When the light exchange material is in the form of a film or sheet, the thickness thereof is not limited, but is preferably 50 μm to 700 μm, more preferably 100 μm to 500 μm.

The light absorbing material is not particularly limited as long as it absorbs at least a part of light in the wavelength range of 700 nm to 1000 nm. It is considered that absorption of light in the wavelength range of 700 nm to 1000 nm suppresses the temperature rise of the cultivation environment and the temperature rise of the culture medium, and promotes rooting. As the light absorbing material, one that shields light in the wavelength range of 900 nm to 1000 nm is preferable.

In the present invention, as the light absorbing material, a material that transmits 60% to 80% of visible light is preferable.

As the light absorbing material, for example, a light absorbing layer containing particles having a function of absorbing light in a wavelength range of 700 nm to 1000 nm, such as a sulfur compound and a copper compound, is laminated on at least one surface of a base material made of a transparent resin. The resulting film or sheet can be used. When the light absorbing material is in the form of a film or sheet, its thickness is preferably 10 to 200 μm.

The configuration of the covering material is not particularly limited as long as it can cover at least a part of the cuttings in the step of rooting from the cuttings, for example, a bag-shaped, film-shaped, sheet-shaped or net-shaped light exchange material or light. Examples thereof include those having an absorber, those having a film-shaped, sheet-shaped or net-shaped light exchange material or light absorption material, and a support that supports the light exchange material or the light absorption material. The covering material is a laminated body of a thin film light exchange material or a light absorbing material and a plastic film or the like, and the laminated body has a bag shape, a film shape, a sheet shape or a net shape. Alternatively, the structure may include the laminated body and a support that supports the laminated body.

The manner in which the cuttings are covered with the covering material is not particularly limited. For example, a film-shaped, sheet-shaped or net-shaped light exchanging material or light absorbing material, may cover the cuttings by spreading on the outside or inside of the vinyl house that houses the culture container into which the cuttings are inserted, A film-shaped, sheet-shaped or net-shaped light exchanging material or light absorbing material may be covered on the dome-shaped support to cover the spikes, or inside the bag-shaped light exchanging material or light absorbing material. The cuttings may be covered by accommodating the cuttings together with the culture vessel.

The effect of the present invention is exhibited if the covering material covers the cuttings in the rooting step, but it is obtained by absorbing light in the wavelength range of 400 nm or less and emitting light in the wavelength range of 550 nm to 700 nm. From the viewpoint of making the effect, or the effect obtained by absorbing light in the wavelength range of 700 nm to 1000 nm, more effective, the covering material is preferably one covering the upper side and the side of the cutting, It is more preferable to cover the entire irradiation region of the light radiated to the cuttings.

Further, from the viewpoint of further promoting rooting from the cuttings, the cuttings can be covered with a light-shielding material made of a light-shielding material together with the covering material. The light-shielding material makes it possible to efficiently adjust the inside of the culture container to a light condition and a temperature environment suitable for rooting, for example, during the period from spring to summer when the light intensity is high. The effect of the light-shielding material is exhibited if a part of the cuttings is covered with the light-shielding material, but it is preferable that the entire area of the irradiation region of the light irradiated on the cuttings is covered. The light-shielding material preferably has a light-shielding rate of 30% or more and 70% or less, and more preferably 40% or more and 60% or less. As the light-shielding material, ginseng or a light-shielding sheet for agriculture can be used.

The temperature of the cultivation environment in the step of rooting from the cuttings is preferably 20° C. or higher, more preferably 22° C. or higher, and further preferably 23° C. or higher. The upper limit of the temperature of the cultivation environment is preferably 30° C. or lower, more preferably 28° C. or lower, and further preferably 26° C. or lower.

The time to execute the step of rooting from cuttings is not particularly limited, but it is easy to adjust the cultivation temperature and the medium temperature to an appropriate temperature, and from the viewpoint that the facility can be simplified, in Japan April to September is preferred. In the present invention, a light exchange material that absorbs at least a part of light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 nm to 700 nm, or light that absorbs at least a part of light having a wavelength range of 700 nm to 1000 nm. Since the cuttings are covered with a covering material equipped with an absorbent material, the temperature of the cultivation environment rises excessively even if the rooting process is performed in an outdoor greenhouse such as a greenhouse when the amount of ultraviolet rays is high and the outside air temperature is high. Can be suppressed.

Although the period of the step of rooting from cuttings varies depending on the plant species, it is usually 2 weeks to 3 months, preferably 4 weeks to 2 months. The step of rooting from the cuttings may be continued until rooting is observed from the cuttings.

The cycle of repeating the light period and the dark period of irradiation is not particularly limited, but it is preferable that the light period is 14 to 18 hours per 24 hours/the dark period is 6 to 10 hours.

In the step of rooting from cuttings, the distribution of light period and dark period is usually 1:1 to 3:1 and preferably 3:2 to 5:2.

The present invention is described below with reference to examples, but the present invention is not limited thereto.

(Example 1)
From the current year's branch of Yoshino cherry tree, a branch to be a cutting was collected, and this branch was cut 2 cm below the bottom leaf to adjust the cutting. Using a cell tray as a culture vessel, Akadama small grain soil (manufactured by Yanshima Shoji Co., Ltd.) was filled and inserted to adjust the floor. The basal portion (cutting portion) of the cuttings prepared as described above was diluted 4-fold with Oxyberon (registered trademark) liquid (Bayer Crop Science Co., Ltd., plant hormone containing IBA, concentration of plant hormone was 1 mg/l. ) Was soaked for 10 seconds, and the cuttings up to 1.5 cm from the base were placed on the cutting bed and placed in an outdoor rooting facility and cultured for 4 weeks. The only light source was sunlight. The average temperature inside the rooting equipment was 20°C, and the average outside temperature was 20°C. Water was used as the medium. As rooting equipment, in order from the outside (outside air side), a light-shielding material (manufactured by Daio Kasei Co., Ltd., gauze cloth, light-shielding rate 70%) and a covering material (sheet-shaped light absorbing material, Mitsubishi Plastics Agridream Co., Ltd.) Manufactured by "Megacool (registered trademark)", and a dome-shaped support was used to fabricate a tunnel-shaped one (details will be described later). The light exchanging material was arranged so as to cover the upper and lateral sides of the cuttings. The cuttings after culturing were visually observed, and if roots were confirmed, it was judged that rooting had occurred. Table 1 shows the number of cuttings (number of cuttings) in each test plot, the number of roots per root, the rooting rate, and the survival rate after 4 weeks.

(Example 2)
Instead of the sheet-shaped light absorbing material, a sheet-shaped light exchange material (manufactured by Material Science Co., Ltd., "Scarlet (registered trademark)") was used in the same manner as in Example 1 except that the covering material was used. The cuttings were cultured for 4 weeks.

(Comparative Example 1)
The cuttings were cultured for 4 weeks in the same manner as in Example 1 except that the covering material was not used.

Regarding "Megacool" used as the light absorbing material, it was confirmed by the following method that at least a part of light having a wavelength range of 700 nm to 1000 nm was absorbed among the irradiated light. Using a self-recording spectrophotometer (manufactured by Shimadzu Corporation), the total light transmittance of the light applied to "Megacool" was measured. As a result, the transmittance of light having a wavelength of 700 nm was 65.1%, the transmittance of light having a wavelength of 850 nm was 50.3%, and the transmittance of light having a wavelength of 1000 nm was 71.2%.

Moreover, it was confirmed that the "scarlet" used as the light exchange material absorbs at least a part of light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 nm to 700 nm by the following method. Using a self-recording spectrophotometer (manufactured by Shimadzu Corporation), the total light transmittance of the light with which the "Scarlet" was irradiated was measured. As a result, the transmittance of light of wavelength 300 nm is 12.0%, the transmittance of light of wavelength 400 nm is 60.5%, the transmittance of light of wavelength 550 nm is 74.3%, and the transmittance of light of wavelength 650 nm is The transmittance of light having a wavelength of 103.0% and 700 nm was 92%.

The rooting equipment used in the examples will be described. The rooting equipment 1 has a tunnel shape, in which cuttings 6 inserted into a floor 5 are placed. The floor 5 is placed on the ground 7. The rooting equipment 1 includes a covering material 2 (a light converting material or a light absorbing material) that covers the entire upper and lateral sides of the cuttings 5, and a light shielding material 4 that is arranged on the covering material 2. .. The covering material 2 is put on a dome-shaped support body 3. The support 3 includes a semicircular portion and a rod-shaped portion to which the ends of the semicircular portion are joined (see FIG. 2 ). In FIG. 2, the left and right ends of the rooting equipment 1 are open, but when the rooting method of the embodiment is executed, the openings are covered with the covering material 2 and the light shielding material 4.

As shown in Table 1, the rooting rate and survival rate of Examples 1 and 2 were higher than those of Comparative Example 1. From these results, it was found that the present invention can improve the rooting rate and survival rate of cuttings. Further, according to the present invention, a light exchange material that absorbs at least a part of light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 to 700 nm, or at least a part of light having a wavelength range of 700 nm to 1000 nm. It was found that the cutting can be rooted from the cuttings by covering the cuttings with a covering material having a light-absorbing material, which simplifies the equipment.

(Example 3)
As cuttings, in place of Yoshino cherry tree, a branch to be cut from the current year branch of Kent (apple) was collected, and cuttings prepared by cutting the branch 2 cm below the bottom leaf were used. The cuttings were cultured in the same manner as in Example 1 except that the culture period was 8 weeks and the number of cuttings was 38. Table 2 shows the number of cuttings (number of cuttings) of each test plot, the number of roots per each test plot after 8 weeks, the rooting rate, and the survival rate. The average temperature inside the rooting equipment was 21°C, and the outside air temperature was 21°C on average.

(Example 4)
The same as Example 3 except that a sheet-shaped light exchanging material (manufactured by Material Science, “Scarlet”) was used as the covering material instead of the sheet-shaped light absorbing material, and the number of cuttings was 104. Then, the cuttings were cultured for 8 weeks.

(Comparative example 2)
The cuttings were cultured for 8 weeks in the same manner as in Example 3 except that the covering material was not used and the number of cuttings was 104.

As shown in Table 2, the rooting rate and survival rate of Examples 3 and 4 were higher than that of Comparative Example 2. From this result, it was found that according to the present invention, the rooting rate and survival rate of cuttings can be improved even in Kent (apple) which is less rooted than Yoshino cherry. Further, according to the present invention, a light exchange material that absorbs at least a part of light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 nm to 700 nm, or at least a part of light having a wavelength range of 700 nm to 1000 nm. It was found that the cutting can be rooted from the cuttings by covering the cuttings with a covering material having a light-absorbing material, which simplifies the equipment.

(Example 5)
As cuttings, instead of Yoshino cherry tree, cuttings from the current year's branch of Albita (eucalyptus) were collected, and cuttings prepared by cutting the branches 2 cm below the bottom leaf were used. The cuttings were cultured in the same manner as in Example 1 except that the culture period was 8 weeks and the number of cuttings was 20. Table 3 shows the number of cuttings (number of cuttings) in each test plot, the number of roots per test plot after 8 weeks, the rooting rate, and the survival rate.

(Example 6)
The cuttings were cultured for 8 weeks in the same manner as in Example 5 except that a sheet-shaped light exchange material (manufactured by Material Science, "Scarlet") was used as the covering material instead of the sheet-shaped light absorber.

(Comparative example 3)
The cuttings were cultured for 8 weeks in the same manner as in Example 5 except that the covering material was not used.

As shown in Table 3, the rooting rate and survival rate of Examples 5 and 6 were higher than those of Comparative Example 3. From this result, it was found that according to the present invention, the rooting rate and survival rate of cuttings can be improved even in arbita (Eucalyptus), which has conventionally been difficult to root. Further, according to the present invention, a light exchange material that absorbs at least a part of light having a wavelength range of 400 nm or less and emits light having a wavelength range of 550 nm to 700 nm, or at least a part of light having a wavelength range of 700 nm to 1000 nm. It was found that the cutting can be rooted from the cuttings by covering the cuttings with a covering material having a light-absorbing material, which simplifies the equipment.

1: Rooting equipment 2: Covering material 3: Support material 4: Shading material 5: Cutting floor 6: Cutting ear 7: Ground

Claims (7)

In the process of rooting from cuttings,
Of the natural light that is radiated to the cuttings, it absorbs at least part of the light in the wavelength range of 400 nm or less and emits it as light in the wavelength range of 550 nm to 700 nm, and
A rooting method of causing rooting from the cuttings by absorbing at least a part of light having a wavelength range of 700 nm to 1000 nm among natural light radiated to the cuttings. In the process of rooting from cuttings,
Of the natural light that is radiated to the cuttings, it absorbs at least part of the light in the wavelength range of 400 nm or less, emits light in the wavelength range of 550 nm to 700 nm , and absorbs at least part of the light in the wavelength range of 700 nm to 1000 nm. The rooting method according to claim 1, wherein at least a part of the cuttings is covered with a covering material having a light absorbing material, and the cuttings are rooted. The rooting method according to claim 2 , wherein the cuttings are covered with a covering material and a light-shielding material arranged so as to overlap the covering material. The rooting method according to claim 2 or 3 , wherein the cutting material is covered on the upper side and the lateral side of the cuttings. The rooting method according to any one of claims 1 to 5 , wherein the cuttings are cuttings of a plant selected from cherry, apple, eucalyptus, cedar and pine. Rooting equipment for rooting from cuttings,
A covering material covering at least a part of the cuttings is provided,
Of the natural light with which the cuttings are irradiated, the covering material absorbs at least a part of light having a wavelength range of 400 nm or less, emits light having a wavelength range of 550 nm to 700 nm , and at least light having a wavelength range of 700 nm to 1000 nm. Rooting equipment that has a light absorbing material that absorbs part of it. The rooting equipment according to claim 6 , further comprising a light-shielding material that covers the cuttings together with the covering material.

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