CN113853962A - Method for cultivating evodia rutaecarpa seedlings - Google Patents
Method for cultivating evodia rutaecarpa seedlings Download PDFInfo
- Publication number
- CN113853962A CN113853962A CN202111213621.4A CN202111213621A CN113853962A CN 113853962 A CN113853962 A CN 113853962A CN 202111213621 A CN202111213621 A CN 202111213621A CN 113853962 A CN113853962 A CN 113853962A
- Authority
- CN
- China
- Prior art keywords
- cutting
- evodia rutaecarpa
- cultivating
- seedlings
- months
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001078983 Tetradium ruticarpum Species 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 42
- 238000005520 cutting process Methods 0.000 claims abstract description 178
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 50
- 238000002791 soaking Methods 0.000 claims abstract description 29
- 239000003617 indole-3-acetic acid Substances 0.000 claims abstract description 25
- 230000008569 process Effects 0.000 claims abstract description 9
- 238000011282 treatment Methods 0.000 claims description 19
- 238000012258 culturing Methods 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 52
- 241000196324 Embryophyta Species 0.000 abstract description 17
- 230000012010 growth Effects 0.000 abstract description 11
- 239000002689 soil Substances 0.000 description 18
- 230000000694 effects Effects 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 240000002234 Allium sativum Species 0.000 description 7
- 235000004611 garlic Nutrition 0.000 description 7
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 239000004576 sand Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000011284 combination treatment Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 208000004998 Abdominal Pain Diseases 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 239000002985 plastic film Substances 0.000 description 4
- 229920006255 plastic film Polymers 0.000 description 4
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 206010047700 Vomiting Diseases 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000002002 slurry Substances 0.000 description 3
- 238000011269 treatment regimen Methods 0.000 description 3
- 230000008673 vomiting Effects 0.000 description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 2
- 241000209020 Cornus Species 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000001914 calming effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000556 factor analysis Methods 0.000 description 2
- 231100000869 headache Toxicity 0.000 description 2
- 239000003864 humus Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 235000019362 perlite Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 238000009333 weeding Methods 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 206010000060 Abdominal distension Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 240000000425 Chaenomeles speciosa Species 0.000 description 1
- 235000005078 Chaenomeles speciosa Nutrition 0.000 description 1
- 241001312741 Gekko swinhonis Species 0.000 description 1
- 241000231392 Gymnosiphon Species 0.000 description 1
- 206010019909 Hernia Diseases 0.000 description 1
- 206010049077 Hernia pain Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010067171 Regurgitation Diseases 0.000 description 1
- 206010038776 Retching Diseases 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 206010047601 Vitamin B1 deficiency Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000002894 beriberi Diseases 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- KBDSLGBFQAGHBE-MSGMIQHVSA-N limonin Chemical compound C=1([C@H]2[C@]3(C)CC[C@H]4[C@@]([C@@]53O[C@@H]5C(=O)O2)(C)C(=O)C[C@@H]2[C@]34COC(=O)C[C@@H]3OC2(C)C)C=COC=1 KBDSLGBFQAGHBE-MSGMIQHVSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000002362 mulch Substances 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 208000011726 slow pulse Diseases 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0256—Ground coverings
- A01G13/0268—Mats or sheets, e.g. nets or fabrics
- A01G13/0275—Films
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for cultivating evodia rutaecarpa seedlings, which comprises the steps of cutting slips in a seedbed and cultivating the cuttings; soaking the cutting end of the cutting slip in an indoleacetic acid solution, wherein the indoleacetic acid solution contains 150-250 ppm of indoleacetic acid, and the soaking time is 10-20 hours; in the cultivation process, the seedbed is covered with a mulching film. The inventor finds that if the cutting slips can survive for more than 8 months after cutting, the subsequent growth of the plants is relatively stable, so that 5 to 8 months after cutting is a key period for improving the overall survival condition of the evodia rutaecarpa seedlings, and the key period for improving the survival rate of the cutting propagation evodia rutaecarpa seedlings is to ensure that the cutting slips can survive in the key period. The inventor further finds that the death of the cutting slips in the period of 5 months to 8 months after cutting can be effectively reduced by properly soaking the cutting ends of the cutting slips with indoleacetic acid with proper concentration and covering mulching films for cultivation after cutting, so that the survival rate is improved.
Description
Technical Field
The invention belongs to the technical field of medicinal material planting, and relates to a method for cultivating evodia rutaecarpa seedlings.
Background
The fructus evodiae is dry near-mature fruit of Rutaceae plant fructus evodiae (Evodia rutaecarpa), Gekko Swinhonis (Euodia rutaecarpa var. officinalis) or Evodia rutaecarpa (Euodia rutaecarpa var. bodinieri). Is spherical or slightly pentagonal oblate spherical, and the diameter is 2 mm-5 mm; the surface is dark yellow green to brown, coarse, and has a plurality of punctiform convex or concave oil spots; the top end of the fruit is provided with a pentagram-shaped crack, and the base part of the fruit is left with fruit stalks with yellow fuzz; hard and crisp, 5 chambers of ovary can be seen in the cross section, and 1 faint yellow seed is arranged in each chamber; strong fragrance, pungent and bitter taste.
The fructus evodiae is a commonly used traditional Chinese medicine with the effects of warming the middle-jiao, pungent, bitter and hot in taste, entering liver, spleen, stomach and kidney meridians, and has the effects of dispelling cold, relieving pain, calming adverse-rising energy, stopping vomiting, supporting yang and stopping diarrhea. The Chinese pharmacopoeia records that the medicine has the effects of dispelling cold, relieving pain, calming the adverse-rising energy, arresting vomiting, supporting yang and stopping diarrhea, and is mainly used for treating jueyin headache, cold hernia and abdominal pain, cold dampness and beriberi, abdominal pain during menstruation, abdominal distension and pain, vomiting and acid regurgitation and morning diarrhea. The "Shangshi Lun" (treatise on Cold-induced diseases) records Evodia rutaecarpa decoction: it is combined with Sheng Jiang and ren Shen to treat Jueyin headache, retching and spitting, white tongue coating and slow pulse. Qi-directing decoction from Yi Fang Jian Yi: it is combined with Xiao Hui Xiang, Chuan Lian Zi and mu Xiang to treat hernia of cold and abdominal pain. Jiming san from Zhu Shi Zhu Ji Yi Fang of Classification: it is combined with mu Gua, Su Ye and Bing Lang to treat cold-damp arthralgia or uprush into abdomen.
In view of the medicinal value, the evodia rutaecarpa has been artificially cultivated and propagated by the main methods including root cutting propagation, tillering propagation, branch cutting propagation and seed propagation. Wherein, the branch and cut propagation mainly comprises: before the early spring plant germinates, 1-2 years of robust branches without diseases and insect pests are selected, spring shoots growing in the years are preferably cut into small sections of about 20cm, each section is provided with 2-3 buds, the upper end cut is 2cm away from bud eyes, and the lower end cross section is oblique. Before cuttage, the base of the cutting is soaked in 300ppm of naphthylacetic acid or indoleacetic acid for 14 hours, or soaked in 100ppm of 2,4-D for 24 hours, so that rooting is facilitated, and the survival rate is improved. During cuttage, the cutting branches are obliquely captured on a seedbed according to the row spacing of 25-30 cm and the plant spacing of 10-13 cm, the soil penetration depth is about 13 cm, and then the soil is covered and compacted. Watering and shading after the cutting, and keeping the soil moist and the relative humidity of the ambient air to be about 83 percent. After one week, new leaves of the shoot tips grow out by successive cutting, and at the moment, a proper amount of nitrogen fertilizer can be sprayed for top dressing of the leaf surfaces. Rooting after 1-2 months, and sprouting new branches from overground buds. The height of the seedlings in the current year can reach 0.6-1 m, and the seedlings can be transplanted in the next year.
Compared with other artificial breeding, the branch cutting breeding has obvious advantages, including: the propagation is fast, the damage to the mother plant is small, the result is early, and therefore the method is widely applied. For example:
a method for culturing the seedlings of evodia rutaecarpa adopts a branch cutting propagation method (also called a branch cutting method) in the asexual propagation of the evodia rutaecarpa and comprises the following steps: a. selecting fertile and loose soil with good drainage for a cutting seedbed, deeply ploughing for 25-30 cm, finely raking and leveling, and making a 150 cm-wide high furrow, wherein the furrow width is 30cm, and the depth is 15cm for later use; b. selecting a strong single plant evodia rutaecarpa which has vigorous growth vigor for 4-5 years, developed root system and no plant diseases and insect pests as a parent plant between 11 months and 2 months of the next year in the current year, taking annual lignified branches, cutting the annual lignified branches into cutting slips with the length of 20-25 cm, keeping 3-4 buds of each cutting slip, cutting the upper end of each cutting slip flat, cutting the lower end of each cutting slip into an inclined plane of 45 degrees, and bundling 50 cutting slips; c. soaking a 1-2 cm cut for 12 hours by adding 1g of rooting agent into 5kg of water, taking out, airing and cutting; or using 500 × 10-6 rooting powder or 500 × 10 rooting powder-6Quickly dipping a 1-2 cm lower cut with a rooting agent solution such as naphthylacetic acid and the like, taking out, airing and cutting; d. the method comprises the following steps of (1) selecting a hole to insert a branch according to the row spacing of 10cm multiplied by 10cm on a cloudy day, leaving 1 bud at the upper end of the branch to expose a soil surface (reverse insertion is forbidden), compacting a matrix after cuttage, watering thoroughly, watering at any time to avoid soil drying, and watering not too much to avoid branch rot; e. when the sprout sprouts 3-4 months later, the shed is built to shade (the shed is 50cm high, and is uncovered at night and in cloudy days) and is not removed until 7 months later, and when the seedling is 20-25 cm high, the seedling is applied thinlyManaging thin human and animal excreta and weeding; f. the amount of cuttings is 6 ten thousand for each mu (677m2), and the cuttings are transplanted and planted after the leaves fall in winter in the first 11 months and before the evodia germinates in the next 3 months, and the cuttings are planted in early spring best.
A method for raising evodia rutaecarpa seedlings by cutting comprises the following steps: (1) cutting annual strong and disease and pest-free evodia stems into cuttings with the length of 25cm and 2-3 bud eyes; (2) soaking for 6-10 hours by using phytohormone; (3) a ditch with the depth of 18cm is cut on the finished sand bed by a small shovel, the processed cuttings are obliquely placed into the ditch according to the plant spacing of 6cm, the cuttings and the sand surface form 40 degrees, sand is covered, the depth of the cuttings in the sand is 2/3 of the depth of the cuttings, and the row spacing is 13 cm; (4) drenching the water for 2 times every day after the cutting to keep the moisture of the matrix at 55-60%; (5) using a sunshade net to construct a shed to shade and avoid direct sunlight, wherein the temperature in the shed is 26-30 ℃; (6) and continuously uncovering the sunshade net after the cuttings germinate and root, and transplanting the cuttings after the cuttings are cultivated for 10-12 months.
A dogwood hardwood cutting rapid propagation seedling raising method comprises the following steps: (1) selecting a cutting: in the middle and late ten days of 5 months, 1-2-year-old robust branches with 3-4 buds are cut from a dogwood tree, two ends of the branches are cut into horseshoe shapes, the horseshoe-shaped cuts are 3-4 cm away from the buds, and the branches are wrapped by preservative films; (2) preparing garlic sterilization slurry: 1-1.2 kg of garlic: 2-3 kg of yellow mud: mixing 12-15 kg of water, fully beating garlic into mashed garlic, fully dissolving the mashed garlic in the water, adding yellow mud, and fully stirring to obtain garlic sterilized slurry for later use; (3) and (3) cutting treatment: respectively inserting the two ends of the cutting in the step (1) into the garlic sterilizing slurry for 5min, and covering the two ends of the cutting with preservative films for later use; (4) preparing a seedbed: the seedbed matrix adopts humus soil, fine sand, vermiculite and perlite, and comprises the following components in percentage by weight: 30% of humus soil, 30% of fine sand, 30% of vermiculite and 10% of perlite, wherein the thickness of the seedbed matrix is 25-30 cm; (5) cuttage: fixing the cutting into a U shape by using a rubber band, inserting the cutting and the rubber band into a seedbed for 8-10 cm, keeping the temperature of the seedbed at 20-25 ℃, inserting two ends of the cutting into soil, enabling a bud to be close to the soil surface and enter the soil for 1-2 cm, ridging and compacting, reserving 2-3 buds on the ground, covering an agricultural film after sufficient water is poured, and removing the film after the buds on the ground surface germinate for 1-2 cm; (6) and (3) water and fertilizer management: when 2-3 buds grow on each cutting, cutting off a U-shaped branch to enable each end to grow a plant, watering once after cutting, spraying 0.5% of monopotassium phosphate after the cut is healed after 3-5 days, and dressing 0.5% of urea once after the autumn comes into the cutting seedling; and in the last ten days of 3 months in the next year, the seedlings are lifted, transplanted and fixedly planted, and the fixedly planted seedlings and the mother branches are buried in the soil together, so that the survival rate is ensured.
However, the survival rate of the evodia rutaecarpa seedlings is very low by adopting the traditional cutting propagation method. In actual production, the survival rate of the evodia rutaecarpa cutting seedlings is in a low level of 30%, and the low survival rate of cutting becomes a main reason for limiting mass propagation and industrial large-scale production of plants. Therefore, a cutting propagation method with high survival rate of the evodia rutaecarpa seedlings is urgently needed to be researched, which has great significance for planting the evodia rutaecarpa.
Disclosure of Invention
Based on the background technology, the invention mainly aims to provide the method for cultivating the evodia rutaecarpa seedlings, and the survival rate of the cuttings is high when the method is adopted for cultivating the evodia rutaecarpa seedlings.
The purpose of the invention can be realized by the following technical scheme:
the invention provides a method for cultivating evodia rutaecarpa seedlings, which comprises the steps of cutting slips on a seedbed and cultivating the cuttings;
the cutting end of the cutting slip is soaked in an indoleacetic acid solution for cutting, the indoleacetic acid solution contains 150 ppm-250 ppm of indoleacetic acid, and the soaking time is 15 h-20 h;
in the process of culturing, the seedbed is covered with a mulching film.
In one embodiment, the diameter of the cutting slip is 0.8 cm-2 cm.
In one embodiment, the indoleacetic acid solution contains 180ppm to 215ppm indoleacetic acid, the soaking time is 15 hours to 18 hours, and the diameter of the cutting shoot is 0.8cm to 1.5 cm.
In one embodiment, the length of the cuttage end immersed in the indoleacetic acid solution is 8-12 cm during the immersion process.
In one embodiment, the time for covering the plastic film is 6 months to 8 months.
In one embodiment, the length of the cutting is 15 cm-20 cm.
In one embodiment, the number of the bud points on the cutting is more than 2.
In one embodiment, the upper cutting opening of the cutting slip is at an angle of 40-50 degrees, and the upper cutting opening of the cutting slip is flush.
In one embodiment, the distance between the upper cut of the cutting and the bud point on the adjacent cutting is 1 cm-2 cm, and the distance between the lower cut of the cutting and the bud point on the adjacent cutting is 1 cm-2 cm.
In one embodiment, the seedbed is shaded during the cultivation.
In one embodiment, the shading treatment comprises the erection of a shading net.
In one embodiment, the cutting shoot is taken from 1.5-2.5 years of age of evodia rutaecarpa.
Compared with the prior art, the invention has the following beneficial effects:
in the long-term research on the cutting propagation of the evodia rutaecarpa seedlings, the inventor finds that the traditional cutting propagation method may show a situation of high survival rate in a short period after cutting, but the situation is not maintained, and the situation that the survival rate is rapidly reduced easily occurs in a later period (for example, in a period from 5 months to 8 months after cutting of cutting slips), so that the overall survival rate of the cutting propagation is low. If the survival time of the cutting slips after cutting exceeds 8 months, the subsequent growth of the plants is stable, therefore, 5 to 8 months after cutting is a key period for improving the overall survival condition of the evodia rutaecarpa seedlings, and the key period for improving the survival rate of the evodia rutaecarpa seedlings after cutting is to ensure that the cutting slips can survive in the key period. In contrast, the inventor finds that the death of the cutting slips in the period of 5 months to 8 months after cutting can be effectively reduced by properly soaking the cutting ends of the cutting slips with indoleacetic acid with proper concentration and covering mulching films after cutting for cultivation, so that the survival rate is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a graph showing a comparison of the survival of seedlings covered or not covered with a plastic film in different treatment protocols as measured on day 26 of 5 months 2020 in accordance with an embodiment of the present invention;
FIG. 2 is a graph showing a comparison of the survival rate of seedlings covered or not covered with plastic film in different treatment schemes of 8/26/2020 according to an embodiment of the present invention;
FIG. 3 is a graph showing a comparison of the survival rate of seedlings covered or not covered with plastic film in different treatment schemes of the present invention measured at 26/11/2020;
FIG. 4 is a graph comparing the effect of length of IAA soak on seedling survival as measured in various treatment regimens at 26/5/2020 for example of the present invention;
FIG. 5 is a graph comparing the effect of length of IAA soak on seedling survival as measured in various treatment regimens at 26/8/2020 for example of the present invention;
FIG. 6 is a graph comparing the effect of length of IAA soak on seedling survival as measured in various treatment regimens on day 26 of 11 months 2020 for examples of the invention.
In fig. 1, 2 and 3: 1 is 200ppmIAA + the thick branch is soaked for 1 h; 2 is 200ppmIAA + fine branch soaking for 1 h; 3 is 'rooting powder + thick branch'; 4 is 200ppmIAA + thick branch soaking for 16 h; 5 is 200ppmIAA + fine branch soaking for 16 h; 6 is '50 ppmIAA + soaking for 0.5 h'; and 7 is blank processing.
Detailed Description
In order to facilitate an understanding of the present invention, the present invention will be described in more detail below. It should be understood, however, that the present invention may be embodied in many different forms and should not be construed as being limited to the embodiments or examples set forth herein. Rather, these embodiments or examples are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments or examples only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of two or more of the associated listed items, including any and all combinations of two or more of the associated listed items, or all of the associated listed items.
The invention provides a method for cultivating evodia rutaecarpa seedlings, which comprises the steps of cutting slips on a seedbed and cultivating the cuttings;
the cutting end of the cutting slip is soaked in an indoleacetic acid solution for cutting, the indoleacetic acid solution contains 150 ppm-250 ppm (including but not limited to the following concentration or any range between the following concentrations: 150ppm, 170ppm, 190ppm, 200ppm, 215ppm, 225ppm and 250ppm) of indoleacetic acid, and the soaking time is 15 h-20 h (including but not limited to the following time or any range between the following times: 15h, 16h, 18h and 20 h);
in the process of culturing, the seedbed is covered with a mulching film.
In the long-term research on the cutting propagation of the evodia rutaecarpa seedlings, the inventor finds that the traditional cutting propagation method may show a situation of high survival rate in a short period after cutting, but the situation is not maintained, and the situation that the survival rate is rapidly reduced easily occurs in a later period (for example, in a period from 5 months to 8 months after cutting of cutting slips), so that the overall survival rate of the cutting propagation is low. If the survival time of the cutting slips after cutting exceeds 8 months, the subsequent growth of the plants is stable, therefore, 5 to 8 months after cutting is a key period for improving the overall survival condition of the evodia rutaecarpa seedlings, and the key period for improving the survival rate of the evodia rutaecarpa seedlings after cutting is to ensure that the cutting slips can survive in the key period. In contrast, the inventor finds that the death of the cutting slips in the period of 5 months to 8 months after cutting can be effectively reduced by properly soaking the cutting ends of the cutting slips with indoleacetic acid with proper concentration and covering mulching films after cutting for cultivation, so that the survival rate is improved. Meanwhile, the growth of weeds in the seedbed can be effectively reduced by the mulching of the mulching film, and the effect is very obvious compared with the effect of the mulching film which is not covered. The measure saves a large amount of labor cost for later weeding.
In the cultivation process, the seedbed is required to be covered with the mulching film, the mulching film can be covered before cuttage, and the mulching film can also be covered after cuttage.
The diameter of the cutting is not particularly limited in the method of the present invention, and the method is applicable to cutting, for example, the diameter of the cutting is 0.8cm to 2 cm.
In one example, the indoleacetic acid solution contains 180ppm to 215ppm indoleacetic acid, the soaking time is 15 hours to 18 hours, and the diameter of the cutting slips is 0.8cm to 1.5 cm.
In one example, the length of the skewer end immersed in the indoleacetic acid solution during the soaking process is 8cm to 12cm, including but not limited to the following length values or a range between these length values: 8cm, 9cm, 10cm, 11cm and 12 cm.
The mulching film, namely the ground covering film, is usually a transparent or black PE film, also has green and silver films, is used for ground covering, and has the functions of improving the soil temperature, keeping the soil moisture, maintaining the soil structure, preventing pests from attacking crops and diseases and the like caused by certain microorganisms, and promoting the growth of plants.
In one example, the mulch film is a black PE film.
The specific length of the cutting shoot is not particularly limited in the invention, and the cutting shoot can be suitable for cutting, for example, the length of the cutting shoot can be controlled to be 15 cm-20 cm, including but not limited to the following length values or ranges between the lengths: 15cm, 16cm, 17cm, 18cm, 19cm, 20 cm.
The number of the shoot points carried by the cutting shoot is not particularly limited, and the number of the shoot points is suitable for cutting, for example, the number of the shoot points on the cutting shoot can be controlled to be more than 2 (preferably 2 to 3), including but not limited to 2, 3, 4 and 5.
The cut of the cutting slip is not particularly limited, and the cutting slip is suitable for cutting, the upper cut of the cutting slip can be at an angle of 40-50 degrees (including but not limited to 40 degrees, 41 degrees, 42 degrees, 43 degrees, 44 degrees, 45 degrees, 46 degrees, 47 degrees, 48 degrees, 49 degrees and 50 degrees), and the upper cut of the cutting slip can be flush.
The distance between the cut on the cutting and the adjacent bud is not particularly limited, and the distance suitable for cutting can be kept, the distance between the upper cut of the cutting and the adjacent bud on the cutting can be controlled to be 1 cm-2 cm (including but not limited to 1cm, 1.5cm, 2cm or the range between the values), and the distance between the lower cut of the cutting and the adjacent bud on the cutting can be controlled to be 1 cm-2 cm (including but not limited to 1cm, 1.5cm, 2cm or the range between the values).
In one example, the seedbed is shaded during the cultivation. The specific mode of the shading treatment is not particularly limited, the shading treatment can be directly carried out on the seedbeds one by one, the unified shading treatment can also be carried out on the nurseries where the seedbeds are located, the shading treatment can be realized by adopting a mode of setting up a black shading net, and other optional feasible modes can also be adopted.
The present invention is not particularly limited to the tree age of the stock plant of the cutting shoot, and may be applied to the cutting of the cutting shoot, and for example, 1.5 to 2.5 years of fructus evodiae may be selected as the stock plant, including but not limited to 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4 and 2.5 years.
Examples
1. Experimental materials and methods
1.1 time and place of experiment
All the cuttings obtained in the experiment are planted in a standardized planting base for the medicinal evodia rutaecarpa in Stone town of plateau of Lechang, Guangdong province in 12 and 26 days in 2019, and black shading nets are erected in nursery gardens during the whole experiment period for shading treatment.
1.2 materials of the experiment
Selection of materials: the cutting materials of the evodia rutaecarpa (E.rutaearpa) used in the experiment are all from healthy plants of 2 ages, and robust branches which are vigorous in growth, full in development and free of diseases and insect pests are selected. Collected in a standardized planting base of the medicinal evodia fruit in the plateau of Lechang, Guangdong province.
Cutting the cutting slips: selecting cut branches, cutting the branches according to two sections of a base part and an upper (tip) part, wherein the length of a cutting shoot is 15 cm-20 cm, more than 2 points with buds are cut, the cut at the upper end is cut horizontally, the cut at the lower end is cut obliquely at an angle of about 45 degrees, the cut is smooth, the cut at the upper end and the cut at the lower end are 1 cm-2 cm away from the buds, and every 50 branches are bundled into one bundle. The cutting is mainly selected from two types, namely a thick branch and a thin branch, wherein the thick branch is the cutting with the diameter of 0.8 cm-2 cm, and the thin branch is the cutting with the diameter of less than 0.8 cm.
Soaking treatment: the bundle is taken as a unit, the soaking depth is about 10cm below the cutting, so that all the cuttings are ensured to be soaked uniformly and sufficiently, and the soaking time is determined according to the design of an experimental scheme.
1.3 design of the experiment
According to the actual planting condition of the evodia rutaecarpa, selecting factors such as the thickness of branches, the type of a rooting agent, the concentration of the rooting agent, the soaking time, the mulching film coverage and the like for investigation, and designing 14 treatment schemes in total, wherein the specific experimental scheme is shown in table 1.
And counting survival data and the like regularly after cuttage, wherein the emergence rate of the fructus evodiae is calculated according to the emergence number of 5 months after planting, then counting survival number every 3 months, and inspecting the survival rate to one age.
TABLE 1 different treatment protocols for Evodia rutaecarpa cuttage
2. Results and analysis of the experiments
2.1 survival statistics
The survival number of the evodia rutaecarpa seedlings is counted three times respectively at 26 days 5 and 26 days 8 and 26 days 11 and 26 days 2020, and the corresponding survival rate is obtained after calculation, which is shown in table 2.
As can be seen from the table, the survival rate of the evodia rutaecarpa has a large reduction range from 5 months to 8 months, the survival rate of the evodia rutaecarpa has little difference from 8 months to 11 months, the growth of the evodia rutaecarpa is basically stable after 8 months of cuttage, the death rate is low, and the control of the growth environment needs to be particularly paid attention in the seedling stage of several months before the survival rate is low, and the like.
TABLE 2 survival rate of cuttings of Evodia rutaecarpa treated differently
2.2 optimum conditions
From table 2, it can be seen that treatment combination 5: the emergence rate and the survival rate of the combination of 200ppm IAA, thick branch soaking for 16h and mulching film are far higher than those of other combinations, the germination rate is up to 98% at the age of 5 months, the survival rate still reaches 70% at the age of nearly one month, and the survival rate is far more than 30% of the average survival rate at present, so that the combination is the best condition.
2.3 Single factor analysis
Further performing single factor analysis, the fact that whether the film is covered or not and the IAA soaking time are main influence factors of the survival rate of the cutting propagation of the evodia rutaecarpa is found.
With the combination of table 2, the combination of different treatments was paired in groups with the mulching as a single factor, and the transformation resulted in fig. 1, 2, and 3, from which it can be seen that the survival rate of the treatment combination with mulching was significantly higher than that of the treatment combination without mulching under the same other conditions. Therefore, the field cuttage survival rate of the evodia rutaecarpa is greatly influenced by the mulching.
With the combination of table 2, the IAA soaking treatment time is taken as a single factor, different treatment combinations are subjected to group pairing, and transformation is carried out to obtain fig. 4, fig. 5 and fig. 6, and it can be seen from the figures that under the condition of consistent other conditions, the influence of the IAA soaking treatment time on the survival rate is not greatly different in the first 6 months of the growth of the cutting seedlings, but when the cutting seedlings grow to the 8 th month, the survival rate of the soaking treatment for 16h is obviously higher than that of the soaking treatment for 1h, and the vitality is stronger.
No regular connection is found between the thickness of branches and other single factors and the survival rate of the evodia rutaecarpa seedlings.
In conclusion, the optimal condition of the treatment combination 5 can be clearly verified by analyzing from the perspective of single factor, and the optimal levels of the two main influencing factors are superposed together, so that the survival rate of the cutting propagation of the evodia rutaecarpa can be well improved.
3. Summary and discussion
By exploring a plurality of factors, the invention gropes the optimal cutting propagation conditions of the evodia rutaecarpa as follows: 200ppmIAA, the thick branches are soaked for 16h and the mulching film is adopted, and the propagation survival rate is greatly influenced by the mulching of the mulching film and the soaking time of the IAA. The cuttage propagation technology of the evodia rutaecarpa seedlings is a good supplement, has certain necessity in the scientific theory, and has certain guiding significance in the actual planting of the evodia rutaecarpa base.
The mulching of the mulching film in modern agricultural production is widely applied, and the mulching film has the advantages that firstly, the moisture of the soil is kept, and the temperature of the soil is kept in winter, so that the effect of resisting cold winter is achieved, secondly, the growth of weeds can be reduced, and the weeds are prevented from competing for nutrients required by the growth of seedlings. The fructus evodiae is shrub, and the nutritional ingredients required by seedling emergence at the early stage come from the cutting ears, so that the nutrition is sufficient. Therefore, for evodia rutaecarpa, the greatest advantage of mulching is the maintenance of soil moisture and temperature.
The above-mentioned embodiments only express several embodiments of the present invention, so as to understand the technical solutions of the present invention specifically and in detail, but not to be understood as the limitation of the protection scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. It should be understood that the technical solutions provided by the present invention, which are obtained by logical analysis, reasoning or limited experiments, are within the scope of the present invention as set forth in the appended claims. Therefore, the protection scope of the present invention should be subject to the content of the appended claims, and the description and the drawings can be used for explaining the content of the claims.
Claims (10)
1. A method for cultivating evodia rutaecarpa seedlings is characterized by comprising the steps of cutting slips in a seedbed and cultivating the cuttings;
the cutting end of the cutting slip is soaked in an indoleacetic acid solution for cutting, the indoleacetic acid solution contains 150 ppm-250 ppm of indoleacetic acid, and the soaking time is 15 h-20 h;
in the process of culturing, the seedbed is covered with a mulching film.
2. The method for cultivating evodia rutaecarpa seedlings according to claim 1, wherein the diameter of the cutting is 0.8cm to 2 cm.
3. The method for cultivating evodia rutaecarpa seedlings according to claim 1 or 2, wherein the indoleacetic acid solution contains 180ppm to 215ppm indoleacetic acid, the soaking time is 15h to 18h, and the diameter of the cutting shoot is 0.8cm to 1.5 cm.
4. The method for cultivating evodia rutaecarpa seedlings according to claim 1 or 2, wherein the length of the cuttage end immersed in the indoleacetic acid solution during the immersion process is 8cm to 12 cm.
5. The method for cultivating evodia rutaecarpa seedlings according to claim 1 or 2, wherein the mulching time is 6 to 8 months.
6. The method for cultivating evodia rutaecarpa seedlings according to claim 1 or 2, wherein the length of the cutting is 15cm to 20 cm.
7. The method for cultivating evodia rutaecarpa seedlings according to claim 1 or 2, wherein the number of bud points on the cutting is more than 2.
8. The method for cultivating fructus evodiae seedlings according to claim 1 or 2, wherein the upper cut of the cutting is at an angle of 40-50 degrees and is level.
9. The method for cultivating evodia rutaecarpa seedlings according to claim 1 or 2, wherein the distance between the upper cut of the cutting and the bud point on the adjacent cutting is 1cm to 2cm, and the distance between the lower cut of the cutting and the bud point on the adjacent cutting is 1cm to 2 cm.
10. The method for cultivating evodia rutaecarpa seedlings according to claim 1 or 2, wherein in the cultivation process, the seedbed is shaded; optionally, the shading treatment mode comprises setting up a shading net;
optionally, the cutting slips are taken from 1.5-2.5 years of fructus evodiae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111213621.4A CN113853962A (en) | 2021-10-19 | 2021-10-19 | Method for cultivating evodia rutaecarpa seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111213621.4A CN113853962A (en) | 2021-10-19 | 2021-10-19 | Method for cultivating evodia rutaecarpa seedlings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113853962A true CN113853962A (en) | 2021-12-31 |
Family
ID=79000334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111213621.4A Pending CN113853962A (en) | 2021-10-19 | 2021-10-19 | Method for cultivating evodia rutaecarpa seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113853962A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145700A (en) * | 2014-08-28 | 2014-11-19 | 广州白云山中一药业有限公司 | Method for planting radix trichosanthis medicine |
| JP2014226096A (en) * | 2013-05-23 | 2014-12-08 | 日本製紙株式会社 | Method for producing plant cutting seedling |
| CN106171420A (en) * | 2016-07-14 | 2016-12-07 | 安徽省石台县七井山食品有限公司 | A kind of Fructus Corni cuttage rapid seedling cultivation method |
| JP2017153393A (en) * | 2016-02-29 | 2017-09-07 | 日本製紙株式会社 | Rooting method and rooting equipment |
| CN109429881A (en) * | 2018-12-26 | 2019-03-08 | 四川省新禾成中药材有限公司 | Evodia rutaecarpa imitated wild breeding implantation methods |
-
2021
- 2021-10-19 CN CN202111213621.4A patent/CN113853962A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014226096A (en) * | 2013-05-23 | 2014-12-08 | 日本製紙株式会社 | Method for producing plant cutting seedling |
| CN104145700A (en) * | 2014-08-28 | 2014-11-19 | 广州白云山中一药业有限公司 | Method for planting radix trichosanthis medicine |
| JP2017153393A (en) * | 2016-02-29 | 2017-09-07 | 日本製紙株式会社 | Rooting method and rooting equipment |
| CN106171420A (en) * | 2016-07-14 | 2016-12-07 | 安徽省石台县七井山食品有限公司 | A kind of Fructus Corni cuttage rapid seedling cultivation method |
| CN109429881A (en) * | 2018-12-26 | 2019-03-08 | 四川省新禾成中药材有限公司 | Evodia rutaecarpa imitated wild breeding implantation methods |
Non-Patent Citations (4)
| Title |
|---|
| 李继华: "《扦插的原理与应用》", 31 December 1987, 上海科学技术出版社, pages: 79 - 80 * |
| 杨再学等: "速效生根灵浸泡时间对吴茱萸扦插出苗的影响", 《山地农业生物学报》, no. 4, 28 August 2004 (2004-08-28), pages 313 - 314 * |
| 章绍尧等: "《浙江植物志 总论》", 31 December 1993, 浙江科学技术出版社, pages: 120 * |
| 郑玉光等: "全国中医药行业高等教育"十三五"规划教材 中药学专业实验指导", 中国中医药出版社, pages: 350 - 351 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103070001B (en) | Method for safely planting okra at high yield and high quality | |
| CN104396526A (en) | Pseudo-ginseng planting method | |
| CN101536656A (en) | Method of culturing of seedling and standardized planting protection of seeds of shinyleaf yellowhorn | |
| CN113348884B (en) | Malus asiatica semi-hard branch cutting seedling raising method | |
| CN104718963A (en) | Tuber breeding and culturing method of paris polyphylla | |
| CN105230300A (en) | Planting method for melons | |
| CN107896863A (en) | A kind of breeding method of capsicum | |
| CN100577000C (en) | Seawater soilless nutrient solution cultivation method for fresh water vegetables | |
| CN105393736A (en) | Planting method for semen cassia | |
| CN105850286A (en) | Asparagus planting method | |
| CN106856922B (en) | Cutting propagation method of Chinese lobelia | |
| CN115104495A (en) | High-yield high-efficiency pesticide-application-reduction cultivation method for facility asparagus bean | |
| CN107278421A (en) | A kind of seed growing method of P. delavayi | |
| CN108934679B (en) | Root-cutting seedling raising and seedling tube raising method for aralia elata seem | |
| CN100388876C (en) | Five-finger fig root standarded and industrilized seed breeding planting method | |
| CN104206186B (en) | A kind of engrafting method of Sassafras tsumu Hemsl | |
| CN111201932A (en) | High-yield cultivation method for dogwood | |
| CN110313371B (en) | Method for cultivating plateau coronarium | |
| CN107466768A (en) | The method that sealwort nursery carries with cape jasmine set | |
| CN107567995A (en) | A kind of implantation methods of interplanting corn ginger | |
| CN113853962A (en) | Method for cultivating evodia rutaecarpa seedlings | |
| CN1286017A (en) | High-yield cultivating method for Aralia mandshurica | |
| CN104488471A (en) | Honeysuckle planting method | |
| CN108243864A (en) | A kind of fructus citri wilsonii planting technique | |
| CN111492887B (en) | Low-latitude plateau domestication and cultivation method for miracle fruit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211231 |
|
| RJ01 | Rejection of invention patent application after publication |